AiPD: Autoinhibited Protein Database

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Autoinhibitory Domains (AIDs)

The domains or sequence segments that play a crucial role in modulating the activity of the target domain in cis.

Relief Mechanisms

The mechanisms that the autoinhibited state of the protein is relieved.
It contains the post-translational modifications (PTMs), partner binding, ligand binding, cleavage, etc.

Assays

The experimental methods to identify the AIDs when the evidence code of the autoinhibited protein is annotated with 'EV'.
It contains the deletion assay, mutagenesis experiment, split protein assay, peptide inhibitor test, structural anaylsis, etc..

Evidence Codes

The annotations based on the identification of an AID. There are three types of Evidence Codes.

Target Domains

The domains or regions regulated by AID.

Accessory Elements

The regions involved in autoinhibition but does not make direct contact with a target domain or is a regulatory region within a catalytic domain, such as an activation loop within the kinase domain.

Description

The detailed description about the autoinhibted protein.
It describes comprehensive information about autoinhibited protein such as AIDs, target domain, and relief mechanism.

Post-translational Modifications

When the specific residue(s) undergo post-translationally modification (PTM) such as phosphorylation, N-glycosylation, the activity of target domain is restored.

Partner Binding

The autoinhibition is relieved when the partner protein binds to the AID or other regions, leading to the dissociation of the interaction between AID and the target domain.

Ligand Binding

The autoinhibition is relieved by binding of a ligand such as Ca2+ to the AID or other regions.

Cleavage

The autoinhibition is relieved when the AID was cleaved from autoinhibited protein.

Others

Others denotes a relief mechanism that does not belong to any of above criteria.

Deletion Assay

The AID of the protein is identified when the deletion or truncation of a region leads to an enhanced activity of protein.

Mutagenesis Experiment

The AID of the protein is identified when the mutation of a residue leads to enhanced activity of protein. In such cases, the mutated residue or the conserved motif containing the mutated residue is assigned as the AID.

Structural Analysis

The AID(s) was/were identified by comparing the active and inactive forms or the protein, which were determined by X-ray crystallography or similar methods.

Split Protein Assay

The AID of the protein is identified by the interaction between AID and target domain was verified when these elements were split from a protein.

Peptide Inhibitor Test

The AID of the protein is identified when protein is inhibited by a peptide, which exists within the protein.

Experimentally Validated

Evidence code for the AID which is identified by experiment.
The detailed experimental methods are described in Assay category.

Annotated by sequence similarity

Evidence code for the AID which is identified by sequence comparison with AID within homologous proteins.

Predicted by cis-regPred

Evidence code for the AID which is predicted using the disordered AID prediction model (cis-regPred).