X5M5N0

SS
Gene name
wnk-1 (C46C2.1)
Protein name
Serine/threonine-protein kinase WNK
Names
EC 2.7.11.1 , Protein kinase with no lysine 1
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_C46C2.1
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

WNK family protein kinases are large enzymes that contain the catalytic lysine in a unique position compared with all other protein kinases. The activity of the Wnk1 kinase domain is controlled by its autoinhibitory domain, which is relieved through specific mutations of key residues in the domain. Activation of Wnk1 requires autophosphorylaiton of Ser382 within its activation loop, and phosphorylation of Ser378 enhances its kinase activity.

Autoinhibitory domains (AIDs)

637-660 (Autoinhibitory region) SS

Target domain

334-596 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

482-503 (Activation loop from InterPro)

Target domain

334-596 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for X5M5N0

Entry ID Method Resolution Chain Position Source
AF-X5M5N0-F1 Predicted AlphaFoldDB

No variants for X5M5N0

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for X5M5N0

No associated diseases with X5M5N0

3 regional properties for X5M5N0

Type Name Position InterPro Accession
domain Protein kinase domain 334 - 596 IPR000719
active_site Serine/threonine-protein kinase, active site 460 - 472 IPR008271
domain Serine/threonine-protein kinase OSR1/WNK, CCT domain 622 - 686 IPR024678

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Mediates formation and localizes to cytoplasmic membraneless compartment in response to hyperosmotic stress
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
molecular condensate scaffold activity Binding and bringing together two or more macromolecules in contact, permitting those molecules to organize as a molecular condensate.
potassium channel inhibitor activity Binds to and stops, prevents, or reduces the activity of a potassium channel.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

11 GO annotations of biological process

Name Definition
cell volume homeostasis Any process involved in maintaining the steady state of a cell's volume. The cell's volume refers to the three-dimensional space occupied by a cell.
cellular hyperosmotic response Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detection of, or exposure to, a hyperosmotic environment, i.e. an environment with a higher concentration of solutes than the organism or cell.
hyperosmotic response Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detection of, or exposure to, a hyperosmotic environment, i.e. an environment with a higher concentration of solutes than the organism or cell.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
monoatomic ion homeostasis Any process involved in the maintenance of an internal steady state of monoatomic ions within an organism or cell. Monatomic ions (also called simple ions) are ions consisting of exactly one atom.
multicellular organismal-level water homeostasis A chemical homeostatic process involved in the maintenance of a steady state level of water within extracellular body fluids, such as blood, xylem or phloem, of a multicellular organism. This is distinct from maintenance of cellular homeostasis, which occurs within a cell.
negative regulation of sodium ion transport Any process that decreases the frequency, rate or extent of the directed movement of sodium ions (Na+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
non-membrane-bounded organelle assembly The aggregation, arrangement and bonding together of a set of components to form a non-membrane-bounded organelle.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
positive regulation of gene expression Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
positive regulation of potassium ion import across plasma membrane Any process that activates or increases the frequency, rate or extent of potassium ion import across the plasma membrane.

16 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q96J92 WNK4 Serine/threonine-protein kinase WNK4 Homo sapiens (Human) SS
Q9H4A3 WNK1 Serine/threonine-protein kinase WNK1 Homo sapiens (Human) SS
Q9Y3S1 WNK2 Serine/threonine-protein kinase WNK2 Homo sapiens (Human) SS
Q9BYP7 WNK3 Serine/threonine-protein kinase WNK3 Homo sapiens (Human) SS
P83741 Wnk1 Serine/threonine-protein kinase WNK1 Mus musculus (Mouse) SS
Q80UE6 Wnk4 Serine/threonine-protein kinase WNK4 Mus musculus (Mouse) SS
Q3UH66 Wnk2 Serine/threonine-protein kinase WNK2 Mus musculus (Mouse) SS
Q80XP9 Wnk3 Serine/threonine-protein kinase WNK3 Mus musculus (Mouse) SS
Q6R2V0 WNK1 Serine/threonine-protein kinase WNK1 Sus scrofa (Pig) SS
Q7TPK6 Wnk4 Serine/threonine-protein kinase WNK4 Rattus norvegicus (Rat) SS
Q9JIH7 Wnk1 Serine/threonine-protein kinase WNK1 Rattus norvegicus (Rat) EV
Q8LST2 WNK7 Probable serine/threonine-protein kinase WNK7 Arabidopsis thaliana (Mouse-ear cress) PR
Q8RXE5 WNK10 Probable serine/threonine-protein kinase WNK10 Arabidopsis thaliana (Mouse-ear cress) PR
Q944Q0 WNK8 Serine/threonine-protein kinase WNK8 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LVL5 WNK4 Probable serine/threonine-protein kinase WNK4 Arabidopsis thaliana (Mouse-ear cress) PR
Q9STK6 WNK3 Probable serine/threonine-protein kinase WNK3 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MPDSITNGGR PPAPPSSVSS TTASTTGNFG TRRRLVNRIK KVDELHPAQE NPTMGSHWLS
70 80 90 100 110 120
EQERSRLEAV QQDWRRTRPM KFQWTSKKRP DDPTTSSPST VSISNALENS TPSLNNVSSI
130 140 150 160 170 180
TNSSSPFSLS SAATSTASAI IPFTSNVATN HPHLNHHVSR IPQAIVTGGT NGSLPPLLIS
190 200 210 220 230 240
PTSAAAATPL ISGKAGPMSP STGSPINVAA TVLQNAVSSP QHSIFDRSRL NKIPPNTSLA
250 260 270 280 290 300
SSSSPSDAAN NDKPIQQRHS ILSNVRTLTQ AMVNDGPRTL TGDDMDKMVS EEERARKEQE
310 320 330 340 350 360
KREEEEKAAR RIDVEDDFDA QEKPIDKSKN GRFLKFDEEL GRGSFKTVFR GLDTETGVAV
370 380 390 400 410 420
AWCELQESKL NKTERQRFRE EAEMLKDLQH PNIVRFYDYW ESADLCGKRK YIVLVTELMT
430 440 450 460 470 480
SGTLKMYLKR FKRINIKVLK SWCRQILKGL SFLHTRNPPV IHRDLKCDNI FITGTTGSVK
490 500 510 520 530 540
IGDLGLATLK NKSFAKSVIG TPEFMAPEMY EEMYDESVDV YAFGMCLLEM VTGEYPYSEC
550 560 570 580 590 600
MNPATIYRKV ISGVKPECFS RIPAQYPEIR EIIDRCIRVR REERSTVKQL LVDDFFTPED
610 620 630 640 650 660
LIGIRVEIKN RDADLNDLNV EIQMQLRVYD EKKRKQYRFK ENEGLQFAFD IENDSPDEVV
670 680 690 700 710 720
QQMIEQQHIP DEDTRMITKL IKDKVDAFRR DRDHRLLEIK RAKEEEERIR EEAEIKEELR
730 740 750 760 770 780
LRAEAKEKEK ERLEKERLEK KAAAAAAANP NPTPIPPTPA TPHSSAQQQP IPPPLSTQTS
790 800 810 820 830 840
AEIQQSAQQP SVPVTMIANI PAMSPTSAQP QPVLSPTSAA VPVPTTMIHV PKPSEIPVQN
850 860 870 880 890 900
VATTAAPVAA NNVPPSPAPF KTEDIQTPTL AQNTVPRTIS TDASGLVINT PASIASPSPA
910 920 930 940 950 960
PSATDVASTT APVTPAPTPT TTTDGGAAAA STTTENKEEK RKSNKRKVVM EILGCDESRN
970 980 990 1000 1010 1020
FALVSCRLDT SHKSVTFQFA PGTDKPCTIA TKLLAEDCLL KVHVHIVEAQ LGEVIQLINS
1030 1040 1050 1060 1070 1080
DGKKGVGTKL ATVLDPNSTE PPTITAVMPK DSSAATASNT KPKIEIEKTP PTRDASQEPN
1090 1100 1110 1120 1130 1140
NVQVTNVRKV SQESNAESVQ SIPRPGGIIV MSPTNQTDSA PPPTGAAAKP SRFQVTKSAD
1150 1160 1170 1180 1190 1200
PIATPISSSI STATVIPIVA ATPTNITSEP VIVQPITAQV ITHLATPSPV SHSLSSNSSP
1210 1220 1230 1240 1250 1260
SATTHSNMSS IQSTTSVPGR RFTVQPVSQA ESGISSSIST PHPEPTPAIT SCPPPVPSVP
1270 1280 1290 1300 1310 1320
PVVSNGTLNL EVAPKQTPSA TNQNVDTQHS SSTASTATLV SETPATVHVT PISVPAPVQE
1330 1340 1350 1360 1370 1380
PLVIDHHSDV LTQLDSELRK CFQVSGVSHS ASPSTVVESL TSMTPQTIPL ACQTVPASIG
1390 1400 1410 1420 1430 1440
QAPAVIAAAH AASLIPNASV PQSPSRLDAE TGLAGLHEKL EALKMEQDRR EDMGDDAIGT
1450 1460 1470 1480 1490 1500
TTTDGKDEIP IDTLKGLAEA LGKVIHADGR ETTPMPPDHP DLTDASTQQL ISPSNPDVLT
1510 1520 1530 1540 1550 1560
TMSSAVEGSA SSTMIEDIDA STSAVDASMM NSMPPGAQNS TDQIPAAMTL SMDQECAQSM
1570 1580 1590 1600 1610 1620
TSSITRNTTG TKLATFENLE TALSSTLGTH IRQPNAPSSR DETTAPMTPS FTNERIGGGG
1630 1640 1650 1660 1670 1680
GGGATSFSIG TPPSHSPFPV SECDYDLKGQ MDLESEDPEV IQMIVRHRME QHKLLEKQRV
1690 1700 1710 1720 1730 1740
EIERLRSKIR VPRATSVNPE MIGDDEADTT LTALQSALGN ASLSLPASPP PNTEIPDNEG
1750 1760 1770 1780 1790 1800
QHHCNSFRCI GDPNDNVSIV NQIKQRLGII PSSRQSVRSA TSSSPSTPPS SSSAPPKSLS
1810 1820 1830 1840
SPTKSYVSHC SLSIGYGSTA SSEQQQREPS PSATTSSFLS DPATGVIENV