Q9ZRD6

SS
Gene name
YKT61 (At5g58060, K21L19.5, K21L19_40)
Protein name
VAMP-like protein YKT61
Names
AtYKT61 , Geranylgeranylated protein 1 , AtGP1
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT5G58060
EC number
Protein Class

Descriptions

Most SNAREs are permanently anchored to membranes, but the dually lipidated SNARE Ykt6 is found both on intracellular membranes and in the cytoplasm. The SNARE core is autoinhibited by the N-terminal longin domain. <br>The dynamics of Ykt6 are believed to be governed by the reversible palmitoylation of the protein, which cycles Ykt6 between intracellular membranes and the cytoplasm. Palmitoylation of Ykt6 increases the partition coefficient of Ykt6 to membranes, thereby shifting some populations of the protein from the cytosol to cellular membranes. Palmitoylation-mediated membrane insertion will further shift the conformational equilibrium of Ykt6 to the open state. The specific membrane localization and the amount of membrane-associated Ykt6 are predicted to be affected by its reversible palmitoylation machinery in cells, as the majority of Ykt6 exists in the unpalmitoylated cytosolic form

Autoinhibitory domains (AIDs)

7-128 (Longin domain) SS

Target domain

139-199 (v-SNARE coiled-coil homology)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9ZRD6

Entry ID Method Resolution Chain Position Source
AF-Q9ZRD6-F1 Predicted AlphaFoldDB

2 variants for Q9ZRD6

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH00740205 45 V>F No 1000Genomes
tmp_5_23500108_T_G 194 S>A No 1000Genomes

No associated diseases with Q9ZRD6

11 regional properties for Q9ZRD6

Type Name Position InterPro Accession
domain Ephrin receptor ligand binding domain 19 - 201 IPR001090
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 620 - 878 IPR001245
conserved_site Tyrosine-protein kinase, receptor class V, conserved site 177 - 197 IPR001426-1
conserved_site Tyrosine-protein kinase, receptor class V, conserved site 240 - 260 IPR001426-2
domain Sterile alpha motif domain 908 - 975 IPR001660
domain Fibronectin type III 322 - 528 IPR003961
active_site Tyrosine-protein kinase, active site 740 - 752 IPR008266
binding_site Protein kinase, ATP binding site 625 - 651 IPR017441
domain Tyrosine-protein kinase, catalytic domain 619 - 878 IPR020635
domain Ephrin type-B receptor 1, ligand binding domain 20 - 195 IPR034231
domain EphB1, SAM domain 908 - 975 IPR042819

Functions

Description
EC Number
Subcellular Localization
  • Cell membrane ; Lipid-anchor ; Cytoplasmic side
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
SNARE complex A protein complex involved in membrane fusion; a stable ternary complex consisting of a four-helix bundle, usually formed from one R-SNARE and three Q-SNAREs with an ionic layer sandwiched between hydrophobic layers. One well-characterized example is the neuronal SNARE complex formed of synaptobrevin 2, syntaxin 1a, and SNAP-25.

1 GO annotations of molecular function

Name Definition
SNAP receptor activity Acting as a marker to identify a membrane and interacting selectively with one or more SNAREs on another membrane to mediate membrane fusion.

3 GO annotations of biological process

Name Definition
endoplasmic reticulum to Golgi vesicle-mediated transport The directed movement of substances from the endoplasmic reticulum (ER) to the Golgi, mediated by COP II vesicles. Small COP II coated vesicles form from the ER and then fuse directly with the cis-Golgi. Larger structures are transported along microtubules to the cis-Golgi.
protein transport The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
vesicle fusion Fusion of the membrane of a transport vesicle with its target membrane.

8 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P36015 YKT6 Synaptobrevin homolog YKT6 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EV
Q3T000 YKT6 Synaptobrevin homolog YKT6 Bos taurus (Bovine) SS
O15498 YKT6 Synaptobrevin homolog YKT6 Homo sapiens (Human) SS
Q9CQW1 Ykt6 Synaptobrevin homolog YKT6 Mus musculus (Mouse) SS
Q5EGY4 Ykt6 Synaptobrevin homolog YKT6 Rattus norvegicus (Rat) EV
Q9LVM9 YKT62 VAMP-like protein YKT62 Arabidopsis thaliana (Mouse-ear cress) PR
Q6P816 ykt6 Synaptobrevin homolog YKT6 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
Q7ZUN8 ykt6 Synaptobrevin homolog YKT6 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MKITALLVLK CAPEASDPVI LSNASDVSHF GYFQRSSVKE FVVFVGRTVA SRTPPSQRQS
70 80 90 100 110 120
VQHEEYKVHA YNRNGLCAVG FMDDHYPVRS AFSLLNQVLD EYQKSFGESW RSAKEDSNQP
130 140 150 160 170 180
WPYLTEALNK FQDPAEADKL LKIQRELDET KIILHKTIDS VLARGEKLDS LVEKSSDLSM
190
ASQMFYKQAK KTNSCCTIL