Q9ZNT0
Gene name |
SKD1 |
Protein name |
Protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 |
Names |
AtSKD1, Protein VACUOLAR PROTEIN SORTING 4 |
Species |
Arabidopsis thaliana (Mouse-ear cress) |
KEGG Pathway |
ath:AT2G27600 |
EC number |
3.6.4.6: Acting on ATP; involved in cellular and subcellular movement |
Protein Class |
|
Descriptions
(Annotation based on sequence homology with Q9UN37)
VPS4 proteins are AAA+ ATPases required to form multivesicular bodies, release viral particles, and complete cytokinesis. VPS4 proteins act by disassembling ESCRT-III heteropolymers during or after their proposed function in membrane scission. Deleting the N-terminal MIT domain and adjacent linker from VPS4A increases both basal and liposome-enhanced ATPase activity, indicating that these elements play a role in autoinhibiting VPS4A until it encounters ESCRT-III polymers. The interactions between acidic ESCRT-III residues and sequences in VPS4A, in particular in the linker connecting the MIT and AAA+ domains, are involved in regulating the intrinsic autoinhibition of the enzyme.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q9ZNT0
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9ZNT0-F1 | Predicted | AlphaFoldDB |
3 variants for Q9ZNT0
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| tmp_2_11781230_A_T | 2 | Y>F | No | 1000Genomes | |
| tmp_2_11783384_G_C | 366 | G>A | No | 1000Genomes | |
| tmp_2_11783458_C_T | 391 | L>F | No | 1000Genomes |
No associated diseases with Q9ZNT0
3 regional properties for Q9ZNT0
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | High mobility group box domain | 137 - 207 | IPR009071-1 |
| domain | High mobility group box domain | 254 - 322 | IPR009071-2 |
| domain | High mobility group box domain | 378 - 448 | IPR009071-3 |
Functions
| Description | ||
|---|---|---|
| EC Number | 3.6.4.6 | Acting on ATP; involved in cellular and subcellular movement |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| multivesicular body | A type of endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm. |
| multivesicular body membrane | The lipid bilayer surrounding a multivesicular body. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| peroxisome | A small organelle enclosed by a single membrane, and found in most eukaryotic cells. Contains peroxidases and other enzymes involved in a variety of metabolic processes including free radical detoxification, lipid catabolism and biosynthesis, and hydrogen peroxide metabolism. |
| plasmodesma | A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. |
9 GO annotations of biological process
| Name | Definition |
|---|---|
| cell cycle | The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division. |
| endosomal transport | The directed movement of substances mediated by an endosome, a membrane-bounded organelle that carries materials enclosed in the lumen or located in the endosomal membrane. |
| endosome organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of endosomes. |
| potassium ion homeostasis | Any process involved in the maintenance of an internal steady state of potassium ions within an organism or cell. |
| protein transport | The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. |
| sodium ion homeostasis | Any process involved in the maintenance of an internal steady state of sodium ions within an organism or cell. |
| trichome branching | Any process involved in the formation of branches in plant hair cells. An example of this process is found in Arabidopsis thaliana. |
| vacuole organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a vacuole. |
| vesicle-mediated transport | A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane. |
8 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P52917 | VPS4 | Vacuolar protein sorting-associated protein 4 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q0VD48 | VPS4B | Vacuolar protein sorting-associated protein 4B | Bos taurus (Bovine) | SS |
| O75351 | VPS4B | Vacuolar protein sorting-associated protein 4B | Homo sapiens (Human) | PR |
| Q9UN37 | VPS4A | Vacuolar protein sorting-associated protein 4A | Homo sapiens (Human) | EV |
| P46467 | Vps4b | Vacuolar protein sorting-associated protein 4B | Mus musculus (Mouse) | PR |
| Q8VEJ9 | Vps4a | Vacuolar protein sorting-associated protein 4A | Mus musculus (Mouse) | PR |
| Q793F9 | Vps4a | Vacuolar protein sorting-associated protein 4A | Rattus norvegicus (Rat) | PR |
| D0FH76 | VPS4 | Vacuolar protein sorting-associated protein 4 | Bombyx mori (Silk moth) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MYSNFKEQAI | EYVKQAVHED | NAGNYNKAFP | LYMNALEYFK | THLKYEKNPK | IREAITQKFT |
| 70 | 80 | 90 | 100 | 110 | 120 |
| EYLRRAEEIR | AVLDEGGSGP | GSNGDAAVAT | RPKTKPKDGE | GGGKDGEDPE | QSKLRAGLNS |
| 130 | 140 | 150 | 160 | 170 | 180 |
| AIVREKPNIK | WSDVAGLESA | KQALQEAVIL | PVKFPQFFTG | KRRPWRAFLL | YGPPGTGKSY |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LAKAVATEAD | STFFSVSSSD | LVSKWMGESE | KLVSNLFEMA | RESAPSIIFV | DEIDSLCGTR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| GEGNESEASR | RIKTELLVQM | QGVGHNDEKV | LVLAATNTPY | ALDQAIRRRF | DKRIYIPLPE |
| 310 | 320 | 330 | 340 | 350 | 360 |
| AKARQHMFKV | HLGDTPHNLT | EPDFEYLGQK | TEGFSGSDVS | VCVKDVLFEP | VRKTQDAMFF |
| 370 | 380 | 390 | 400 | 410 | 420 |
| FKSPDGTWMP | CGPRHPGAIQ | TTMQDLATKG | LAEKIIPPPI | TRTDFEKVLA | RQRPTVSKSD |
| 430 | |||||
| LDVHERFTQE | FGEEG |