Q9Z330

SS
Gene name
Dnmt1
Protein name
DNA (cytosine-5)-methyltransferase 1
Names
Dnmt1, DNA MTase RnoIP, M.RnoIP, DNA methyltransferase I, MCMT
Species
Rattus norvegicus (Rat)
KEGG Pathway
EC number
2.1.1.37: Methyltransferases
Protein Class

Descriptions

The eukaryotic maintenance DNA methyltransferase DNMT1 is multimodular protein composed of a replication foci-targeting domain (RFD), a DBA-binding CXXC domain, a pair of bromo-adjacent homology (BAH) domains, and a C-terminal catalytic domain, and mediates maintenance of genomic methylation patterns. In a study with mouse DNMT1, the CXXC may specifically bind to unmethylated CpG dinucleotide and position the CXXC-BAH1 linker between the DNA and the active site of DNMT1, preventing de novo methylation. Therefore, unmethylated CpG dinucleotides are occluded from the active site to ensure that only hemimethylated CpG dinucleotides undergo methylation.

Autoinhibitory domains (AIDs)

700-734 (CXXC-BAH1 linker) SS

Target domain

1136-1595 (Methyltransferase domain)

Relief mechanism

Others

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9Z330

Entry ID Method Resolution Chain Position Source
AF-Q9Z330-F1 Predicted AlphaFoldDB

No variants for Q9Z330

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9Z330

No associated diseases with Q9Z330

4 regional properties for Q9Z330

Type Name Position InterPro Accession
domain Zinc finger, CXXC-type 650 - 696 IPR002857
domain DMAP1-binding domain 16 - 109 IPR010506
active_site DNA methylase, C-5 cytosine-specific, active site 1223 - 1235 IPR018117
conserved_site DNA methylase, C-5 cytosine-specific, conserved site 1578 - 1596 IPR031303

Functions

Description
EC Number 2.1.1.37 Methyltransferases
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
heterochromatin A compact and highly condensed form of chromatin that is refractory to transcription.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
pericentric heterochromatin Heterochromatin that is located adjacent to the CENP-A rich centromere 'central core' and characterized by methylated H3 histone at lysine 9 (H3K9me2/H3K9me3).
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
replication fork The Y-shaped region of a replicating DNA molecule, resulting from the separation of the DNA strands and in which the synthesis of new strands takes place. Also includes associated protein complexes.

13 GO annotations of molecular function

Name Definition
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
DNA (cytosine-5-)-methyltransferase activity Catalysis of the reaction: S-adenosyl-L-methionine + DNA containing cytosine = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA-methyltransferase activity Catalysis of the transfer of a methyl group to a DNA molecule.
estrogen receptor binding Binding to a nuclear estrogen receptor.
histone deacetylase binding Binding to histone deacetylase.
methyl-CpG binding Binding to a methylated cytosine/guanine dinucleotide.
methyltransferase activity Catalysis of the transfer of a methyl group to an acceptor molecule.
promoter-specific chromatin binding Binding to a section of chromatin that is associated with gene promoter sequences of DNA.
protein domain specific binding Binding to a specific domain of a protein.
RNA binding Binding to an RNA molecule or a portion thereof.
S-adenosylmethionine-dependent methyltransferase activity Catalysis of the transfer of a methyl group from S-adenosyl-L-methionine to a substrate.
zinc ion binding Binding to a zinc ion (Zn).

44 GO annotations of biological process

Name Definition
aging A developmental process that is a deterioration and loss of function over time. Aging includes loss of functions such as resistance to disease, homeostasis, and fertility, as well as wear and tear. Aging includes cellular senescence, but is more inclusive. May precede death and may succeed developmental maturation (GO:0021700).
brain development The process whose specific outcome is the progression of the brain over time, from its formation to the mature structure. Brain development begins with patterning events in the neural tube and ends with the mature structure that is the center of thought and emotion. The brain is responsible for the coordination and control of bodily activities and the interpretation of information from the senses (sight, hearing, smell, etc.).
cellular response to amino acid stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an amino acid stimulus. An amino acid is a carboxylic acids containing one or more amino groups.
cellular response to lead ion Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lead ion stimulus.
cellular response to nerve growth factor stimulus A process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nerve growth factor stimulus.
cellular response to platelet-derived growth factor stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a platelet-derived growth factor stimulus.
cellular response to transforming growth factor beta stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a transforming growth factor beta stimulus.
chromatin organization The assembly or remodeling of chromatin composed of DNA complexed with histones, other associated proteins, and sometimes RNA.
DNA hypermethylation An increase in the epigenetic methylation of cytosine and adenosine residues in DNA.
DNA methylation The covalent transfer of a methyl group to either N-6 of adenine or C-5 or N-4 of cytosine.
DNA methylation involved in embryo development The covalent transfer of a methyl group to C-5 of cytosine that contributes to the epigenetic regulation of embryonic gene expression.
DNA methylation on cytosine The covalent transfer of a methyl group to C-5 or N-4 of cytosine in a DNA molecule.
DNA methylation on cytosine within a CG sequence The covalent transfer of a methyl group to C-5 or N-4 of a cytosine located within a CG sequence in a DNA molecule.
gene silencing OBSOLETE. Any process carried out at the cellular level that results in either long-term transcriptional repression via action on chromatin structure or RNA mediated, post-transcriptional repression of gene expression.
maintenance of DNA methylation Any process involved in maintaining the methylation state of a nucleotide sequence.
negative regulation of gene expression Any process that decreases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
negative regulation of histone H3-K9 methylation Any process that stops, prevents, or reduces the frequency, rate or extent of the covalent addition of a methyl group to the lysine at position 9 of histone H3.
negative regulation of transcription by RNA polymerase II Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
negative regulation of transcription, DNA-templated Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription.
negative regulation of vascular associated smooth muscle cell apoptotic process Any process that stops, prevents or reduces the frequency, rate or extent of vascular associated smooth muscle cell apoptotic process.
negative regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching Any process that stops, prevents or reduces the frequency, rate or extent of vascular smooth muscle cell differentiation involved in phenotypic switching.
neuron differentiation The process in which a relatively unspecialized cell acquires specialized features of a neuron.
positive regulation of DNA methylation-dependent heterochromatin assembly Any process that increases the rate, frequency, or extent of DNA methylation-dependent heterochromatin formation.
positive regulation of gene expression Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
positive regulation of histone H3-K4 methylation Any process that activates or increases the frequency, rate or extent of the covalent addition of a methyl group to the lysine at position 4 of histone H3.
positive regulation of vascular associated smooth muscle cell proliferation Any process that activates or increases the frequency, rate or extent of vascular smooth muscle cell proliferation.
Ras protein signal transduction The series of molecular signals within the cell that are mediated by a member of the Ras superfamily of proteins switching to a GTP-bound active state.
regulation of cell population proliferation Any process that modulates the frequency, rate or extent of cell proliferation.
regulation of gene expression Any process that modulates the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
response to activity Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an activity stimulus.
response to caffeine Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a caffeine stimulus. Caffeine is an alkaloid found in numerous plant species, where it acts as a natural pesticide that paralyzes and kills certain insects feeding upon them.
response to estradiol Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of stimulus by estradiol, a C18 steroid hormone hydroxylated at C3 and C17 that acts as a potent estrogen.
response to ethanol Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ethanol stimulus.
response to heat Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism.
response to ionizing radiation Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a ionizing radiation stimulus. Ionizing radiation is radiation with sufficient energy to remove electrons from atoms and may arise from spontaneous decay of unstable isotopes, resulting in alpha and beta particles and gamma rays. Ionizing radiation also includes X-rays.
response to lead ion Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lead ion stimulus.
response to lipopolysaccharide Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria.
response to nutrient levels Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus reflecting the presence, absence, or concentration of nutrients.
response to organic substance Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an organic substance stimulus.
response to testosterone Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a testosterone stimulus.
response to toxic substance Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a toxic stimulus.
response to vitamin A Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a vitamin A stimulus.
response to xenobiotic stimulus Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
S-adenosylmethionine metabolic process The chemical reactions and pathways involving S-adenosylmethionine, S-(5'-adenosyl)-L-methionine, an important intermediate in one-carbon metabolism.

5 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q24K09 DNMT1 DNA (cytosine-5)-methyltransferase 1 Bos taurus (Bovine) SS
Q92072 DNMT1 DNA Gallus gallus (Chicken) SS
P26358 DNMT1 DNA (cytosine-5)-methyltransferase 1 Homo sapiens (Human) EV
P13864 Dnmt1 DNA (cytosine-5)-methyltransferase 1 Mus musculus (Mouse) EV
O49139 CMT1 Putative DNA (cytosine-5)-methyltransferase CMT1 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MPARTAPARV PALASPAGSL PDHVRRRLKD LERDGLTEKE CVKEKLNLLH EFLQTEIKSQ
70 80 90 100 110 120
LCDLETKLHK EELSEEGYLA KVKTLLNKDL CLENGTLSLT QKANGCPANG SRPTWKAEMA
130 140 150 160 170 180
DSNRSPRSRP KPRGPRRSKS DSETMIEASS SSVATRRTTR QTTITSHFKG PAKRKPKEDS
190 200 210 220 230 240
EKGNANESAA EERDQDKKRR VAGTESRASR AGESVEKPER VRPGTQLCQE EQGEQEDDRR
250 260 270 280 290 300
PRRQTRELAS RRKSREDPDR EARPGTHLDV DDDDEKDKRS SRPRSQPRDL ATKRRPKEEV
310 320 330 340 350 360
EQITPEPPEG KDEDEREEKR RKTTRKKPEP LSIPVQSRVE RKASQGKASA IPKLNPPQCP
370 380 390 400 410 420
ECGQYLDDPD LKYQQHPVDA VDEPQMLTNE ALSVFDSNSS WFETYDSSPM HKFTFFSVYC
430 440 450 460 470 480
SRGHLCPVDT GLIEKNVELY FSGVAKAIHE ENPSVEGGVN GKNLGPINQW WISGFDGGEK
490 500 510 520 530 540
ALIGFSTAFA EYFLMEPSPE YAPIFGLMQE KIYISKIVVE FLQSNPDAVY EDLINKIETT
550 560 570 580 590 600
VPPSAINVNR FTEDSLLRHA QFVVSQVESY DDAKDDDETP IFLSPCMRSL IHLAGVSLGQ
610 620 630 640 650 660
RRATRRTVIN SAKVKRKGPT KATTTKLVYQ IFDTFFSEQI EKDDKEDKEN TMKRRRCGVC
670 680 690 700 710 720
EVCQQPECGK CKACKDMVKF GGTGRSKQAC LKRRCPNLAV KEADEDEEAD DDIPELPSPK
730 740 750 760 770 780
KLHQGKKKKQ NKDRISWLGE PVKIEENRTY YWKVSIDEET LEVGDCVSVI PDDPSKPLYL
790 800 810 820 830 840
ARVTALWEDK NGQMFHAHWF CAGTDTVLGA TSDPLELFLV GECENMQLSY IHSKVKVIYR
850 860 870 880 890 900
GPSPNWAMEG GMDPEAMLPG AEDGKTYFYQ FWYSQDYARF ESPPKTQPAE DNKHKFCLSC
910 920 930 940 950 960
IRLAELRQKE MPKVLEQLEE VDGRVYCSSI TKNGVVYRLG DSVYLPPEAF TFNIKMASPM
970 980 990 1000 1010 1020
KRSKRDPVNE NPVPRDTYRK YSDYIKGSNL DAPEPYRIGR IKEIYCGKKK GGKVNEADIK
1030 1040 1050 1060 1070 1080
IRLYKFYRPE NTHKSIQATY HADINLLYWS DEEAVVDFSD VQGRCTVEYG EDLLESIQDY
1090 1100 1110 1120 1130 1140
SQGGPDRFYF LEAYNSKTKS FEDPPNHARS PGNKGKGKGK GKGKGKPQVS EPKEPEAAIK
1150 1160 1170 1180 1190 1200
LPKLRTLDVF SGCGGLTEGF HQAGISETLW AIEMWEPAAQ AFRLNNPGTT VFTEDCNVLL
1210 1220 1230 1240 1250 1260
KLVMAGEVTN SLGQRLPQKG DVEMLCGGPP CQGFSGMNRF NSRTYSKFKN SLVVSFLSYC
1270 1280 1290 1300 1310 1320
DYYRPRFFLL ENVRNFVSFR RSMVLKLTLR CLVRMGYQCT FGVLQAGQYG VAQTRRRAII
1330 1340 1350 1360 1370 1380
LAAAPGEKLP LFPEPLHVFA PRACQLSVVV DDKKFVSNIT RLSSGPFRTI TMRDTMSDLP
1390 1400 1410 1420 1430 1440
EIQNGASAPE ISYKWRATVL VPEAAARVAL PAHPQGPYPQ VHERAGGCRM RHIPLSPGSD
1450 1460 1470 1480 1490 1500
WRDLPNIQVR LRDGVITNKL RYTFHDTKNG CSSTGALRGV CSCAEGKTCD PASRQFNTLI
1510 1520 1530 1540 1550 1560
PWCLPHTGNR HNHWAGLYGR LEWDGFFSTT VTNPEPMGKQ GRVLHPEQHR VVSVRECARS
1570 1580 1590 1600 1610 1620
QGFPDTYRLF GNILDRHRQV GNAVPPPLAK AIGLEIKLCL LASAQESASA AVKGKEETTT
ED