Q9Z207
SS
Gene name |
Diaph3 (Diap3) |
Protein name |
Protein diaphanous homolog 3 |
Names |
Diaphanous-related formin-3, DRF3, p134mDIA2, mDIA2 |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:56419 |
EC number |
|
Protein Class |
|
Descriptions
Formins are conserved actin nucleators responsible for the assembly of diverse actin structures. The autoinhibition mechanism involves the intramolecular interaction between N-terminal DID domain and C-terminal DAD domain. Autoinhibition is relieved by GTP-bound Rho proteins, which bind to the DID and an adjacent GTPase binding (G) element, and consequently displace the DAD. The DAD domain was identified by sequence similarity with mDia1 (O08808).
Autoinhibitory domains (AIDs)
Target domain |
160-361 (DID domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Lammers M et al. (2005) "The regulation of mDia1 by autoinhibition and its release by Rho*GTP", The EMBO journal, 24, 4176-87
- Otomo T et al. (2010) "Crystal structure of the Formin mDia1 in autoinhibited conformation", PloS one, 5,
- Watanabe S et al. (2010) "Rho and anillin-dependent control of mDia2 localization and function in cytokinesis", Molecular biology of the cell, 21, 3193-204
- Otomo T et al. (2005) "Structural basis of Rho GTPase-mediated activation of the formin mDia1", Molecular cell, 18, 273-81
- Kitzing TM et al. (2007) "Positive feedback between Dia1, LARG, and RhoA regulates cell morphology and invasion", Genes & development, 21, 1478-83
- Mizuno H et al. (2018) "Helical rotation of the diaphanous-related formin mDia1 generates actin filaments resistant to cofilin", Proceedings of the National Academy of Sciences of the United States of America, 115, E5000-E5007
- Seth A et al. (2006) "Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLalpha and mDia1", The Journal of cell biology, 174, 701-13
- Lash LL et al. (2013) "Small-molecule intramimics of formin autoinhibition: a new strategy to target the cytoskeletal remodeling machinery in cancer cells", Cancer research, 73, 6793-803
- Deacon SW et al. (2008) "Chemical inhibition through conformational stabilization of Rho GTPase effectors", Handbook of experimental pharmacology, , 431-60
- Orshanskiy IA et al. (2015) "[Molecular Dynamics of N- and C-terminal Interactions during Autoinhibition and Activation of Formin mDial]", Biofizika, 60, 451-6
- Lammers M et al. (2008) "Specificity of interactions between mDia isoforms and Rho proteins", The Journal of biological chemistry, 283, 35236-46
- Copeland SJ et al. (2007) "The diaphanous inhibitory domain/diaphanous autoregulatory domain interaction is able to mediate heterodimerization between mDia1 and mDia2", The Journal of biological chemistry, 282, 30120-30
- Alberts AS (2001) "Identification of a carboxyl-terminal diaphanous-related formin homology protein autoregulatory domain", The Journal of biological chemistry, 276, 2824-30
- Maiti S et al. (2012) "Structure and activity of full-length formin mDia1", Cytoskeleton (Hoboken, N.J.), 69, 393-405
- Gould CJ et al. (2011) "The formin DAD domain plays dual roles in autoinhibition and actin nucleation", Current biology : CB, 21, 384-90
- Goode BL et al. (2007) "Mechanism and function of formins in the control of actin assembly", Annual review of biochemistry, 76, 593-627
- Higgs HN (2005) "Formin proteins: a domain-based approach", Trends in biochemical sciences, 30, 342-53
- Nezami A et al. (2010) "Crystal structure of a complex between amino and carboxy terminal fragments of mDia1: insights into autoinhibition of diaphanous-related formins", PloS one, 5,
- Kovar DR (2006) "Molecular details of formin-mediated actin assembly", Current opinion in cell biology, 18, 11-7
- Li F et al. (2005) "Dissecting requirements for auto-inhibition of actin nucleation by the formin, mDia1", The Journal of biological chemistry, 280, 6986-92
- Copeland JW et al. (2004) "Homo-oligomerization is essential for F-actin assembly by the formin family FH2 domain", The Journal of biological chemistry, 279, 50250-6
- Bai CX et al. (2008) "Activation of TRPP2 through mDia1-dependent voltage gating", The EMBO journal, 27, 1345-56
- Li F et al. (2003) "The mouse Formin mDia1 is a potent actin nucleation factor regulated by autoinhibition", Current biology : CB, 13, 1335-40
Autoinhibited structure
Activated structure
1 structures for Q9Z207
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9Z207-F1 | Predicted | AlphaFoldDB |
18 variants for Q9Z207
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs257101779 | 17 | R>W | No | Ensembl | |
| rs212710775 | 89 | M>I | No | Ensembl | |
| rs49858486 | 93 | P>T | No | Ensembl | |
| rs213334036 | 159 | L>I | No | Ensembl | |
| rs1132740960 | 220 | K>* | No | Ensembl | |
| rs225147211 | 315 | N>H | No | Ensembl | |
| rs48675644 | 380 | S>N | No | Ensembl | |
| rs214259276 | 403 | L>V | No | Ensembl | |
| rs45836177 | 409 | E>Q | No | Ensembl | |
| rs50936935 | 550 | G>C | No | Ensembl | |
| rs253894461 | 555 | P>S | No | Ensembl | |
| rs229587155 | 561 | L>F | No | Ensembl | |
| rs265212208 | 570 | P>L | No | Ensembl | |
| rs260632709 | 589 | P>L | No | Ensembl | |
| rs214909203 | 687 | R>K | No | Ensembl | |
| rs246429505 | 901 | V>L | No | Ensembl | |
| rs30389082 | 960 | I>V | No | Ensembl | |
| rs51372759 | 1142 | K>R | No | Ensembl |
No associated diseases with Q9Z207
5 regional properties for Q9Z207
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Formin, FH3 domain | 281 - 469 | IPR010472 |
| domain | Formin, GTPase-binding domain | 93 - 276 | IPR010473 |
| domain | Diaphanous autoregulatory (DAD) domain | 1036 - 1066 | IPR014767 |
| domain | Rho GTPase-binding/formin homology 3 (GBD/FH3) domain | 93 - 455 | IPR014768 |
| domain | Formin, FH2 domain | 615 - 1056 | IPR015425 |
Functions
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| actin binding | Binding to monomeric or multimeric forms of actin, including actin filaments. |
| small GTPase binding | Binding to a small monomeric GTPase. |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| actin cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins. |
| actin filament polymerization | Assembly of actin filaments by the addition of actin monomers to a filament. |
| cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures. |
8 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P48608 | dia | Protein diaphanous | Drosophila melanogaster (Fruit fly) | EV |
| O60879 | DIAPH2 | Protein diaphanous homolog 2 | Homo sapiens (Human) | SS |
| O60610 | DIAPH1 | Protein diaphanous homolog 1 | Homo sapiens (Human) | SS |
| Q9NSV4 | DIAPH3 | Protein diaphanous homolog 3 | Homo sapiens (Human) | SS |
| O08808 | Diaph1 | Protein diaphanous homolog 1 | Mus musculus (Mouse) | EV |
| O70566 | Diaph2 | Protein diaphanous homolog 2 | Mus musculus (Mouse) | SS |
| F1M775 | Diaph1 | Protein diaphanous homolog 1 | Rattus norvegicus (Rat) | SS |
| F1LVW7 | Diaph3 | Protein diaphanous homolog 3 | Rattus norvegicus (Rat) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MERHRARALG | RDSKSSRRKG | LQSAPPAGPY | EPGEKRPKLH | LNIRTLTDDM | LDKFASIRIP |
| 70 | 80 | 90 | 100 | 110 | 120 |
| GSKKERPPLP | HLKTVSGISD | SSSLSSETME | NNPKALPESE | VLKLFEKMME | DMNLNEDKKA |
| 130 | 140 | 150 | 160 | 170 | 180 |
| PLREKDFGIK | KEMVMQYINT | ASKTGSLRSS | RQISPQEFLH | ELKMGYTDER | LFTYLESLRV |
| 190 | 200 | 210 | 220 | 230 | 240 |
| SLTSHPVSWV | QSFGHEGLGL | LLDILEKLIN | GQIQEKVVKK | TQHKVIQCLR | ALMNTQYGLE |
| 250 | 260 | 270 | 280 | 290 | 300 |
| RIMSDKRSLS | LLAKAMDPRQ | PAMMADVVKL | LSAVCIVGEE | SILEEVLEAL | TSAGEERKID |
| 310 | 320 | 330 | 340 | 350 | 360 |
| RFFSIVEGLR | HNSVNLQVAC | MQLINALVTS | PDDLDFRLHL | RNEFMRCGLK | EILPNLKGIK |
| 370 | 380 | 390 | 400 | 410 | 420 |
| NDGLDIQLKV | FDEHKEEDLS | EFFHRLEDIR | AELDEASDVY | SMLWDTVKET | RAEGHFLSIL |
| 430 | 440 | 450 | 460 | 470 | 480 |
| QHLLLIRNDR | FIREQYFKLI | DECVSQIVLH | RDGTDPDFTY | RKRLDLDLSQ | FVDVCIDQAK |
| 490 | 500 | 510 | 520 | 530 | 540 |
| LDEWEEKASE | HCKKFEKECT | DHQETQAQLQ | KREAKINELQ | AELQAFKSQF | GALPPGTKIP |
| 550 | 560 | 570 | 580 | 590 | 600 |
| LQPSVEGEAG | PSALPPAPPA | LSGGVPPPPP | PPPPPPPPLP | GMPMPFGGPV | PPPPPLGFLG |
| 610 | 620 | 630 | 640 | 650 | 660 |
| GQSSIPLNLP | FGLKPKKEFK | PEISMRRLNW | LKIGPNEMSE | NCFWIKVNEN | KYENRDLLCK |
| 670 | 680 | 690 | 700 | 710 | 720 |
| LENTFCCQEK | EKRNTNDFDE | KKVIKKRMKE | LKFLDPKIAQ | NLSIFLSSFR | VPYEKIRTMI |
| 730 | 740 | 750 | 760 | 770 | 780 |
| LEVDETQLSE | SMIQNLIKHL | PDEEQLKSLS | QFRSDYNSLC | EPEQFAVVMS | NVKRLRPRLS |
| 790 | 800 | 810 | 820 | 830 | 840 |
| AILFKLQFEE | QVNNIKPDIM | AVSTACEEIK | KSKGFSKLLE | LVLLMGNYMN | AGSRNAQTFG |
| 850 | 860 | 870 | 880 | 890 | 900 |
| FDLSSLCKLK | DTKSADQKTT | LLHFLVDVCE | EKHADILHFV | DDLAHLDKAS | RVSVEMLEKN |
| 910 | 920 | 930 | 940 | 950 | 960 |
| VKQMGRQLQQ | LEKNLETFPP | PEDLHDKFVI | KMSSFVISAN | EQYEKLSTLL | GSMTQLYQSI |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| MGYYAVDMKK | VSVEEFFNDL | NNFRTSFMLA | LKENIKKREA | AEKEKRARIA | KERAEKERLE |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| RQQEKKRLLE | MKTEGDETGV | MDSLLEALQS | GAAFRDRRKR | TPKLKDIRQS | LSPMSQRPVL |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| KVCNHENQKM | QLTEGSRPHH | SINCNSTRTP | VAKELNYNLD | THASTGRIKA | VEKEACNAES |
| 1150 | 1160 | 1170 | |||
| NKKKEMELLG | SVAKSESVPE | VEALLARLRA | L |