AiPD: Autoinhibited Protein Database

Q9Y4X5

Gene name	ARIH1
Protein name	E3 ubiquitin-protein ligase ARIH1
Names	H7-AP2, HHARI, Monocyte protein 6, MOP-6, Protein ariadne-1 homolog, ARI-1, UbcH7-binding protein, UbcM4-interacting protein, Ubiquitin-conjugating enzyme E2-binding protein 1
Species	Homo sapiens (Human)
KEGG Pathway	hsa:25820
EC number	2.3.2.31: Aminoacyltransferases
Protein Class	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING (PTHR11685)

Descriptions

E3 ubiquitin-protein ligase ARIH1 is an E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3, and acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates. ARIH1 is autoinhibited by the ariadne domain, which masks the second RING-type zinc finger that contains the active site and inhibits the E3 activity. The inhibition is relieved upon binding to neddylated cullin-RING ubiquitin ligase complexes, which activate the E3 ligase activity of ARIH1.

Autoinhibitory domains (AIDs)

Target domain	276-347 (RING2 domain)
Relief mechanism	Partner binding
Assay	Deletion assay, Mutagenesis experiment, Structural analysis

AID

408-557 (Ariadne domain)

Target domain

276-347 (RING2 domain)

Relief mechanism

Partner binding (Binding of Ariadne-binding partner proteins to Ariadne domain)

Assay

Deletion assay, Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Duda DM et al. (2013) "Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism", Structure (London, England : 1993), 21, 1030-41

Kelsall IR et al. (2013) "TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes", The EMBO journal, 32, 2848-60

Autoinhibited structure

Activated structure

11 structures for Q9Y4X5

Entry ID	Method	Resolution	Chain	Position	Source
1WD2	NMR	-	A	336-394	PDB
2M9Y	NMR	-	A	325-396	PDB
4KBL	X-ray	330 A	A/B	1-557	PDB
4KC9	X-ray	360 A	A	1-557	PDB
5TTE	X-ray	350 A	B	1-557	PDB
5UDH	X-ray	324 A	A/B	90-557	PDB
7B5L	EM	380 A	H	1-557	PDB
7B5M	EM	391 A	H	1-557	PDB
7B5N	EM	360 A	H	1-557	PDB
7B5S	EM	360 A	H	1-557	PDB
AF-Q9Y4X5-F1	Predicted				AlphaFoldDB

167 variants for Q9Y4X5

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
VAR_082646 CA273260089 rs1052050835 RCV001291053	15	E>Q	probable disease-associated variant found in patient with an acute aortic dissection and ascending aortic aneurysm Aortic aneurysm [UniProt, ClinVar]	Yes	ClinGen ClinVar UniProt TOPMed dbSNP
rs2063786716 RCV001291431	44	E>G	Aortic aneurysm [ClinVar]	Yes	ClinVar dbSNP
RCV001291430 rs746505361 CA7645516	171	R>*	Aortic aneurysm [ClinVar]	Yes	ClinGen ClinVar ExAC dbSNP gnomAD
rs751344394 CA7645402	7	Y>C		No	ClinGen ExAC TOPMed gnomAD
CA393235936 rs1302485230	7	Y>H		No	ClinGen TOPMed
rs1361234355 CA393235990	8	N>H		No	ClinGen TOPMed
rs781240234 CA7645404	8	N>I		No	ClinGen ExAC TOPMed gnomAD
rs1477796553 CA393236038	9	Y>C		No	ClinGen TOPMed
CA393236051 rs1479803832	10	E>Q		No	ClinGen gnomAD
rs1195230372 CA393236099	11	F>L		No	ClinGen TOPMed
rs769816706 CA7645406	12	D>E		No	ClinGen ExAC TOPMed gnomAD
rs1428522519 CA393236106	12	D>N		No	ClinGen TOPMed
rs779981165 CA7645407	15	E>A		No	ClinGen ExAC gnomAD
rs779981165 CA393236205	15	E>G		No	ClinGen ExAC gnomAD
rs890255198 CA273260090	18	S>C		No	ClinGen gnomAD
rs890255198 CA393236268	18	S>G		No	ClinGen gnomAD
rs1321793234 CA393236299	19	E>K		No	ClinGen gnomAD
rs868117311 CA273260091	21	D>E		No	ClinGen Ensembl
CA7645411 rs768692029	22	S>G		No	ClinGen ExAC gnomAD
rs772456417 CA7645415	25	E>D		No	ClinGen ExAC gnomAD
rs1327083729 CA393236373	25	E>Q		No	ClinGen TOPMed
CA7645416 rs773605993	28	E>D		No	ClinGen ExAC gnomAD
rs1348781230 CA393236418	28	E>G		No	ClinGen gnomAD
CA7645418 rs761158526	29	D>E		No	ClinGen ExAC gnomAD
CA7645417 rs761158526	29	D>E		No	ClinGen ExAC gnomAD
CA7645419 rs752084483	30	E>K		No	ClinGen ExAC TOPMed gnomAD
rs1490087109 CA393236491	32	D>E		No	ClinGen gnomAD
CA393236505 rs1182181311	33	D>E		No	ClinGen gnomAD
CA393236554 rs1253333685	36	D>E		No	ClinGen gnomAD
CA7645421 rs763608232	37	D>G		No	ClinGen ExAC gnomAD
rs751293008 CA7645423	39	T>I		No	ClinGen ExAC gnomAD
CA7645422 rs751293008	39	T>N		No	ClinGen ExAC gnomAD
rs946176693 CA273260094	41	D>N		No	ClinGen TOPMed
rs994449992 CA273260095	45	V>A		No	ClinGen gnomAD
CA7645427 rs780042095	47	L>V		No	ClinGen ExAC TOPMed gnomAD
rs1430641905 CA393236752	49	E>*		No	ClinGen gnomAD
CA7645428 rs749268378	49	E>D		No	ClinGen ExAC gnomAD
CA393236754 rs1595845215	49	E>G		No	ClinGen Ensembl
rs768801971 CA7645429	50	P>S		No	ClinGen ExAC gnomAD
CA273260096 rs1042181947	51	G>W		No	ClinGen TOPMed
rs1312645720 CA393236805	53	G>S		No	ClinGen gnomAD
rs1358544322 CA393236815	54	V>I		No	ClinGen gnomAD
CA393236833 rs1239114198	55	G>A		No	ClinGen TOPMed gnomAD
CA393236840 rs1184730148	56	G>R		No	ClinGen TOPMed
rs1000583730 CA273260097	57	E>Q		No	ClinGen TOPMed
CA7645433 rs773561251	58	R>W		No	ClinGen ExAC gnomAD
rs1177781521 CA393236885	59	D>N		No	ClinGen TOPMed
CA393236943 rs1426082091	64	G>R		No	ClinGen gnomAD
rs777220733 CA7645436	66	T>M		No	ClinGen ExAC gnomAD
CA393236976 rs1450537917	69	G>D		No	ClinGen TOPMed gnomAD
rs1013967251 CA273260099	71	G>S		No	ClinGen Ensembl
CA393236992 rs1264967111	72	S>C		No	ClinGen TOPMed
CA393236999 rs774057607	73	A>P		No	ClinGen ExAC gnomAD
CA7645439 rs774057607	73	A>S		No	ClinGen ExAC gnomAD
rs1303516615 CA393237004	74	L>V		No	ClinGen TOPMed gnomAD
CA7645442 rs559502830	76	P>L		No	ClinGen 1000Genomes TOPMed gnomAD
rs767341707 CA7645441	76	P>T		No	ClinGen ExAC gnomAD
rs1228917143 CA393237018	77	G>S		No	ClinGen gnomAD
rs755947974 CA7645446	78	G>S		No	ClinGen ExAC gnomAD
CA393237049 rs1199225308	82	G>R		No	ClinGen TOPMed gnomAD
CA393237048 rs1199225308	82	G>S		No	ClinGen TOPMed gnomAD
CA393237056 rs1465600303	83	G>C		No	ClinGen gnomAD
rs12902861 CA273260104	84	G>C		No	ClinGen Ensembl
rs753843976 CA7645456	85	G>D		No	ClinGen ExAC gnomAD
CA273260105 rs967532979	86	G>V		No	ClinGen TOPMed gnomAD
rs1417513681 CA393237078	87	G>D		No	ClinGen TOPMed
rs1297238945 CA393237076	87	G>R		No	ClinGen TOPMed
CA273260106 rs977706882	88	G>S		No	ClinGen Ensembl
rs1379429183 CA393237087	89	G>S		No	ClinGen TOPMed gnomAD
RCV002056684 RCV000455671 rs375614248	90	G>missing		No	ClinVar dbSNP
CA393237100 rs1375923364	91	P>S		No	ClinGen TOPMed
rs1464648760 CA393237106	92	G>W		No	ClinGen gnomAD
rs1405301183 CA393237114	93	H>R		No	ClinGen gnomAD
rs1481483194 CA393237120	94	E>Q		No	ClinGen TOPMed
CA273260108 rs957384868	95	Q>H		No	ClinGen TOPMed gnomAD
rs1359687603 CA393237167	100	R>C		No	ClinGen gnomAD
CA7645461 rs368784844	100	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
rs1206162417 CA393237174	101	Y>C		No	ClinGen TOPMed
rs921251553 CA273260109	102	E>K		No	ClinGen gnomAD
CA273260110 rs12903022	107	E>D		No	ClinGen Ensembl
rs1210904578 CA393237232	110	L>V		No	ClinGen gnomAD
CA393237253 rs1191965773	113	M>L		No	ClinGen gnomAD
CA273260112 rs867712471	118	R>L		No	ClinGen Ensembl
CA7645467 rs746369007	121	N>K		No	ClinGen ExAC gnomAD
CA7645469 rs770477644	122	E>D		No	ClinGen ExAC TOPMed gnomAD
rs771736875 CA7645492	130	I>L		No	ClinGen ExAC TOPMed gnomAD
CA393242616 rs771736875	130	I>V		No	ClinGen ExAC TOPMed gnomAD
CA393242655 rs1297406432	136	S>G		No	ClinGen TOPMed
rs147408829 CA273265289	139	N>S		No	ClinGen ESP TOPMed gnomAD
rs1243956076 CA393235448	154	L>V		No	ClinGen gnomAD
rs933413352 CA273268323	162	H>R		No	ClinGen TOPMed
rs769334669 CA7645515	167	S>N		No	ClinGen ExAC TOPMed gnomAD
rs769334669 CA7645514	167	S>T		No	ClinGen ExAC TOPMed gnomAD
CA7645517 rs770559164	171	R>Q		No	ClinGen ExAC gnomAD
rs148941976 CA7645518	173	R>C		No	ClinGen 1000Genomes ExAC gnomAD
rs969696237 CA273268324	173	R>H		No	ClinGen gnomAD
CA393235841 rs979829613	174	Q>H		No	ClinGen Ensembl
rs1251423210 CA393235886	176	N>S		No	ClinGen TOPMed
CA7645519 rs759288383	177	T>A		No	ClinGen ExAC gnomAD
CA7645521 rs775316269	180	S>T		No	ClinGen ExAC TOPMed gnomAD
CA7645523 rs764009252	183	D>N		No	ClinGen ExAC gnomAD
rs1000868639 CA273268326	191	L>V		No	ClinGen TOPMed
CA273268327 rs779405166	192	N>K		No	ClinGen TOPMed
CA393236276 rs1345775312	196	S>L		No	ClinGen gnomAD
CA7645537 rs769496771	197	Y>C		No	ClinGen ExAC gnomAD
rs1295878502 CA393237641	198	F>L		No	ClinGen gnomAD
CA393237676 rs1345038178	202	E>Q		No	ClinGen gnomAD
CA273269551 rs961904194	209	M>L		No	ClinGen TOPMed gnomAD
rs961904194 CA273269550	209	M>V		No	ClinGen TOPMed gnomAD
CA393237876 rs1228878159	213	S>N		No	ClinGen gnomAD
rs1286785152 CA393238218	229	I>T		No	ClinGen TOPMed
CA393238283 rs1343881622	234	H>N		No	ClinGen TOPMed
CA7645560 rs748015574	235	G>D		No	ClinGen ExAC gnomAD
CA393238472 rs1181805525	246	M>V		No	ClinGen TOPMed gnomAD
CA7645576 rs754711191	247	R>C		No	ClinGen ExAC TOPMed
CA7645577 rs778561142	247	R>H		No	ClinGen ExAC TOPMed gnomAD
rs147551270 CA7645578	251	D>E		No	ClinGen ESP ExAC TOPMed gnomAD
rs1392004426 CA393239750	251	D>N		No	ClinGen TOPMed
rs1461577237 CA393239907	262	I>V		No	ClinGen gnomAD
CA393240186 rs1405057056	270	N>S		No	ClinGen gnomAD
rs1454157851 CA393240191	271	R>Q		No	ClinGen gnomAD
rs778633164 CA7645597	272	L>R		No	ClinGen ExAC gnomAD
rs149030231 CA273270415	280	D>G		No	ClinGen ESP
rs1218416217 CA393240249	280	D>H		No	ClinGen TOPMed
rs758299803 CA7645599	283	H>Q		No	ClinGen ExAC gnomAD
rs777749802 CA7645600	288	Q>H		No	ClinGen ExAC gnomAD
CA393240354 rs1271592185	288	Q>R		No	ClinGen TOPMed
CA393240410 rs1339411218	292	A>T		No	ClinGen TOPMed
rs781125737 CA7645603	294	P>A		No	ClinGen ExAC gnomAD
CA393240576 rs1332093999	294	P>L		No	ClinGen TOPMed
rs1259128502 COSM1374415 CA393240585	296	R>C	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
rs1259128502 CA393240584	296	R>G		No	ClinGen TOPMed gnomAD
rs769846251 CA7645605	296	R>H		No	ClinGen ExAC TOPMed gnomAD
rs769846251 CA7645606	296	R>L		No	ClinGen ExAC TOPMed gnomAD
CA393240607 rs1463886403	299	C>S		No	ClinGen TOPMed
rs1417583080 CA393240617	301	R>C		No	ClinGen TOPMed
rs1188543265 CA393240620	301	R>H		No	ClinGen TOPMed
rs1320295436 CA393240801	321	K>N		No	ClinGen TOPMed
CA7645640 rs757137026	326	K>T		No	ClinGen ExAC
CA393240891 rs1595871915	333	T>P		No	ClinGen Ensembl
rs750348642 CA7645642	335	N>S		No	ClinGen ExAC TOPMed gnomAD
rs192253156 CA273271038	357	C>Y		No	ClinGen 1000Genomes
rs969550607 CA273271039	365	Q>*		No	ClinGen Ensembl
rs573902027 CA7645661	370	E>D		No	ClinGen 1000Genomes ExAC gnomAD
rs1284449604 CA393241194	373	W>R		No	ClinGen gnomAD
CA7645686 rs753804913	391	R>H		No	ClinGen ExAC gnomAD
CA7645687 rs759517486	399	A>V		No	ClinGen ExAC gnomAD
CA7645734 rs762126728	406	R>L		No	ClinGen ExAC gnomAD
CA393241459 rs762126728	406	R>Q		No	ClinGen ExAC gnomAD
CA7645735 rs767859277	409	A>E		No	ClinGen ExAC gnomAD
rs753701203 CA7645738	420	R>H		No	ClinGen ExAC gnomAD
rs777142325 CA7645741	443	E>G		No	ClinGen ExAC
CA273272166 rs113869196	459	K>R		No	ClinGen Ensembl
CA7645747 rs779944062	483	K>R		No	ClinGen ExAC gnomAD
rs768710024 CA7645749	484	K>N		No	ClinGen ExAC gnomAD
rs1203862513 CA393242169	503	E>K		No	ClinGen gnomAD
CA7645776 rs753167225	521	I>V		No	ClinGen ExAC gnomAD
rs1447655771 CA393242349	528	K>M		No	ClinGen gnomAD
COSM382806 CA393242415 rs1276382860	535	R>L	lung [Cosmic]	No	ClinGen cosmic curated gnomAD
rs770168604 CA7645801	538	V>G		No	ClinGen ExAC gnomAD
rs1370979485 CA393242436	539	L>S		No	ClinGen TOPMed
rs1218251335 CA393242453	541	Q>H		No	ClinGen gnomAD
rs1213846918 CA393242474	544	H>L		No	ClinGen TOPMed gnomAD
rs1213846918 CA393242472	544	H>R		No	ClinGen TOPMed gnomAD
CA393242495 rs1348294540	547	Y>C		No	ClinGen TOPMed
rs1245309692 CA393242551	554	Y>*		No	ClinGen gnomAD
CA393242570 rs1477923543	557	D>Y		No	ClinGen gnomAD

1 associated diseases with Q9Y4X5

Without disease ID

6 regional properties for Q9Y4X5

Type	Name	Position	InterPro Accession
domain	Zinc finger, RING-type	186 - 234	IPR001841-1
domain	Zinc finger, RING-type	276 - 347	IPR001841-2
domain	IBR domain	256 - 317	IPR002867-1
domain	IBR domain	325 - 389	IPR002867-2
domain	TRIAD supradomain	182 - 393	IPR044066
domain	Ariadne domain	403 - 524	IPR045840

Functions

		Description
EC Number	2.3.2.31	Aminoacyltransferases
Subcellular Localization	Cytoplasm Nucleus Nucleus, Cajal body	Mainly cytoplasmic (PubMed:11278816) Present in Lewy body (PubMed:21590270)
PANTHER Family	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING
PANTHER Subfamily	PTHR11685:SF212	E3 UBIQUITIN-PROTEIN LIGASE ARIH1
PANTHER Protein Class	ubiquitin-protein ligase protein modifying enzyme
PANTHER Pathway Category	No pathway information available

7 GO annotations of cellular component

Name	Definition
Cajal body	A class of nuclear body, first seen after silver staining by Ramon y Cajal in 1903, enriched in small nuclear ribonucleoproteins, and certain general RNA polymerase II transcription factors; ultrastructurally, they appear as a tangle of coiled, electron-dense threads roughly 0.5 micrometers in diameter; involved in aspects of snRNP biogenesis; the protein coilin serves as a marker for Cajal bodies. Some argue that Cajal bodies are the sites for preassembly of transcriptosomes, unitary particles involved in transcription and processing of RNA.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
Lewy body	Cytoplasmic, spherical inclusion commonly found in damaged neurons, and composed of abnormally phosphorylated, neurofilament proteins aggregated with ubiquitin and alpha-synuclein.
nuclear body	Extra-nucleolar nuclear domains usually visualized by confocal microscopy and fluorescent antibodies to specific proteins.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
ubiquitin ligase complex	A protein complex that includes a ubiquitin-protein ligase and enables ubiquitin protein ligase activity. The complex also contains other proteins that may confer substrate specificity on the complex.

6 GO annotations of molecular function

Name	Definition
ubiquitin conjugating enzyme binding	Binding to a ubiquitin conjugating enzyme, any of the E2 proteins.
ubiquitin protein ligase activity	Catalysis of the transfer of ubiquitin to a substrate protein via the reaction X-ubiquitin + S -> X + S-ubiquitin, where X is either an E2 or E3 enzyme, the X-ubiquitin linkage is a thioester bond, and the S-ubiquitin linkage is an amide bond: an isopeptide bond between the C-terminal glycine of ubiquitin and the epsilon-amino group of lysine residues in the substrate or, in the linear extension of ubiquitin chains, a peptide bond the between the C-terminal glycine and N-terminal methionine of ubiquitin residues.
ubiquitin protein ligase binding	Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.
ubiquitin-like protein transferase activity	Catalysis of the transfer of a ubiquitin-like from one protein to another via the reaction X-ULP + Y --> Y-ULP + X, where both X-ULP and Y-ULP are covalent linkages. ULP represents a ubiquitin-like protein.
ubiquitin-protein transferase activity	Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.
zinc ion binding	Binding to a zinc ion (Zn).

4 GO annotations of biological process

Name	Definition
positive regulation of proteasomal ubiquitin-dependent protein catabolic process	Any process that activates or increases the frequency, rate or extent of the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome.
protein polyubiquitination	Addition of multiple ubiquitin groups to a protein, forming a ubiquitin chain.
protein ubiquitination	The process in which one or more ubiquitin groups are added to a protein.
ubiquitin-dependent protein catabolic process	The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein.

12 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
A2VEA3	ARIH1	E3 ubiquitin-protein ligase ARIH1	Bos taurus (Bovine)	SS
Q94981	ari-1	E3 ubiquitin-protein ligase ariadne-1	Drosophila melanogaster (Fruit fly)	SS
O76924	ari-2	Potential E3 ubiquitin-protein ligase ariadne-2	Drosophila melanogaster (Fruit fly)	SS
O60260	PRKN	E3 ubiquitin-protein ligase parkin	Homo sapiens (Human)	EV
O95376	ARIH2	E3 ubiquitin-protein ligase ARIH2	Homo sapiens (Human)	EV
Q9Z1K5	Arih1	E3 ubiquitin-protein ligase ARIH1	Mus musculus (Mouse)	SS
Q9Z1K6	Arih2	E3 ubiquitin-protein ligase ARIH2	Mus musculus (Mouse)	SS
O01965	ari-1.1	E3 ubiquitin-protein ligase ari-1.1	Caenorhabditis elegans	SS
Q22431	ari-2	Potential E3 ubiquitin-protein ligase ariadne-2	Caenorhabditis elegans	SS
B1H1E4	arih1	E3 ubiquitin-protein ligase arih1	Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)	SS
Q6NW85	arih1l	E3 ubiquitin-protein ligase arih1l	Danio rerio (Zebrafish) (Brachydanio rerio)	SS
Q6PFJ9	arih1	E3 ubiquitin-protein ligase arih1	Danio rerio (Zebrafish) (Brachydanio rerio)	SS

10	20	30	40	50	60
MDSDEGYNYE	FDEDEECSEE	DSGAEEEEDE	DDDEPDDDTL	DLGEVELVEP	GLGVGGERDG
70	80	90	100	110	120
LLCGETGGGG	GSALGPGGGG	GGGGGGGGGG	PGHEQEEDYR	YEVLTAEQIL	QHMVECIREV
130	140	150	160	170	180
NEVIQNPATI	TRILLSHFNW	DKEKLMERYF	DGNLEKLFAE	CHVINPSKKS	RTRQMNTRSS
190	200	210	220	230	240
AQDMPCQICY	LNYPNSYFTG	LECGHKFCMQ	CWSEYLTTKI	MEEGMGQTIS	CPAHGCDILV
250	260	270	280	290	300
DDNTVMRLIT	DSKVKLKYQH	LITNSFVECN	RLLKWCPAPD	CHHVVKVQYP	DAKPVRCKCG
310	320	330	340	350	360
RQFCFNCGEN	WHDPVKCKWL	KKWIKKCDDD	SETSNWIAAN	TKECPKCHVT	IEKDGGCNHM
370	380	390	400	410	420
VCRNQNCKAE	FCWVCLGPWE	PHGSAWYNCN	RYNEDDAKAA	RDAQERSRAA	LQRYLFYCNR
430	440	450	460	470	480
YMNHMQSLRF	EHKLYAQVKQ	KMEEMQQHNM	SWIEVQFLKK	AVDVLCQCRA	TLMYTYVFAF
490	500	510	520	530	540
YLKKNNQSII	FENNQADLEN	ATEVLSGYLE	RDISQDSLQD	IKQKVQDKYR	YCESRRRVLL
550
QHVHEGYEKD	LWEYIED