AiPD: Autoinhibited Protein Database

Q9Y3E7

Gene name	CHMP3 (CGI149, NEDF, VPS24)
Protein name	Charged multivesicular body protein 3
Names	Chromatin-modifying protein 3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24
Species	Homo sapiens (Human)
KEGG Pathway	hsa:51652
EC number
Protein Class	CHARGED MULTIVESICULAR BODY PROTEIN (PTHR10476)

Descriptions

CHMP3 is a core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The autoinhibition of CHMP3 is regulated through the intramolecular interactions between N-terminal basic domain and C-terminal acidic domain. The binding of the endosome-associated ubiquitin isopeptidase (AMSH) to the acidic half relieves the autoinhibition of CHMP3. CHMP3 can be induced to block HIV-1 release (Anti-HIV-1 budding activity) by coexpressing AMSH or by truncating the acidic domain.

Autoinhibitory domains (AIDs)

Target domain	1-150 (N-terminal domain)
Relief mechanism	Partner binding
Assay	Deletion assay, Mutagenesis experiment, Structural analysis

AID

151-222 (C-terminal autoinhibitory region)

Target domain

1-150 (N-terminal domain)

Relief mechanism

Partner binding (Binding of AMSH to the acidic half of CHMP3)

Assay

Deletion assay, Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Zamborlini A et al. (2006) "Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding", Proceedings of the National Academy of Sciences of the United States of America, 103, 19140-5

Bajorek M et al. (2009) "Structural basis for ESCRT-III protein autoinhibition", Nature structural & molecular biology, 16, 754-62

Autoinhibited structure

Activated structure

7 structures for Q9Y3E7

Entry ID	Method	Resolution	Chain	Position	Source
2GD5	X-ray	280 A	A/B/C/D	9-183	PDB
2XZE	X-ray	175 A	Q/R	183-222	PDB
3FRT	X-ray	400 A	A/B	8-222	PDB
3FRV	X-ray	370 A	A	1-150	PDB
7ZCG	EM	330 A	A	11-169	PDB
7ZCH	EM	360 A	A	11-169	PDB
AF-Q9Y3E7-F1	Predicted				AlphaFoldDB

155 variants for Q9Y3E7

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA347565883 rs1382997162	2	G>A		No	ClinGen gnomAD
CA347565851 rs1231402215	5	G>E		No	ClinGen TOPMed
rs1286901822 CA347565857	5	G>R		No	ClinGen TOPMed gnomAD
rs1406757066 CA347565843	6	K>E		No	ClinGen gnomAD
rs1253519911 CA347565822	7	T>I		No	ClinGen gnomAD
rs774481900 CA1750268	7	T>S		No	ClinGen ExAC TOPMed gnomAD
rs1468136327 CA347565820	8	Q>E		No	ClinGen gnomAD
rs759443052 COSM286472 CA1750267	9	E>D	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs1425230936 CA347565804	9	E>K		No	ClinGen gnomAD
rs1558667032 CA347565784	10	K>E		No	ClinGen Ensembl
CA1750264 rs776458208	10	K>R		No	ClinGen ExAC gnomAD
rs1259181723 CA347565770	11	P>A		No	ClinGen gnomAD
rs770666607 CA1750263	12	P>A		No	ClinGen ExAC TOPMed gnomAD
rs770666607 CA51427674	12	P>S		No	ClinGen ExAC TOPMed gnomAD
CA347565726 rs1558667007	15	L>M		No	ClinGen Ensembl
CA1750226 rs760712170	17	N>S		No	ClinGen ExAC TOPMed gnomAD
CA347564628 rs760712170	17	N>T		No	ClinGen ExAC TOPMed gnomAD
CA347564595 rs1238312283	19	W>*		No	ClinGen gnomAD
CA347564574 rs1333848460	20	S>L		No	ClinGen gnomAD
CA347564583 rs1430538203	20	S>P		No	ClinGen TOPMed gnomAD
rs540948421 CA1750224	23	I>M		No	ClinGen ExAC TOPMed gnomAD
CA51413409 rs1019126612	26	E>A		No	ClinGen Ensembl
CA51413413 rs548843054	26	E>K		No	ClinGen 1000Genomes
CA1750223 rs761505549	30	V>I		No	ClinGen ExAC gnomAD
rs774159726 CA1750222	31	D>E		No	ClinGen ExAC TOPMed gnomAD
CA51413402 rs374031204	31	D>V		No	ClinGen ESP
rs144255258 CA1750220	35	R>K		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA1750202 rs757120612	36	D>G		No	ClinGen ExAC gnomAD
rs1158342289 CA347564357	36	D>N		No	ClinGen gnomAD
CA347563102 rs1475865871	37	I>S		No	ClinGen gnomAD
rs572378103 CA1750200	38	Q>R		No	ClinGen 1000Genomes ExAC gnomAD
rs762716183 CA1750199	39	R>T		No	ClinGen ExAC gnomAD
rs774832261 CA1750198	40	E>K		No	ClinGen ExAC TOPMed gnomAD
rs1204304017 CA347563063	43	K>E		No	ClinGen gnomAD
rs1390555044 CA347563060	43	K>R		No	ClinGen TOPMed
rs764835867 CA1750197	44	V>M		No	ClinGen ExAC TOPMed gnomAD
rs139320001 CA1750196	46	R>*		No	ClinGen ESP ExAC TOPMed gnomAD
rs139320001 CA1750195	46	R>G		No	ClinGen ESP ExAC TOPMed gnomAD
rs747374601 CA347563043	46	R>L		No	ClinGen ExAC gnomAD
CA1750193 rs747374601	46	R>P		No	ClinGen ExAC gnomAD
COSM197615 rs747374601 CA1750194	46	R>Q	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs1238197243 CA347563034	48	V>L		No	ClinGen TOPMed gnomAD
rs1339697638 CA347563023	49	K>N		No	ClinGen gnomAD
rs773687427 CA1750192	51	A>V		No	ClinGen ExAC gnomAD
rs888272079 CA51406098	52	A>T		No	ClinGen Ensembl
rs139264120 CA1750191	52	A>V		No	ClinGen ESP ExAC TOPMed gnomAD
CA347562979 rs1239678603	56	Q>R		No	ClinGen TOPMed
rs778806637 CA1750189	57	K>N		No	ClinGen ExAC gnomAD
CA347562966 rs1573263744	58	D>N		No	ClinGen Ensembl
CA347562928 rs1479872895	64	A>T		No	ClinGen TOPMed
rs1004476833 CA51406055	65	K>T		No	ClinGen TOPMed
rs745732049 CA1750187	67	M>T		No	ClinGen ExAC TOPMed gnomAD
rs377602911 CA1750186	68	I>N		No	ClinGen ExAC TOPMed gnomAD
rs1171339253 CA347562862	73	A>V		No	ClinGen TOPMed
rs897082944 CA51406027	75	S>R		No	ClinGen Ensembl
rs1172942486 CA347562840	76	K>N		No	ClinGen gnomAD
rs757322286 CA1750185	78	Y>C		No	ClinGen ExAC TOPMed gnomAD
CA51406011 rs764294745	80	S>F		No	ClinGen TOPMed gnomAD
CA347562799 rs1244810285	83	H>Y		No	ClinGen TOPMed gnomAD
CA1750181 rs752418529	84	M>L		No	ClinGen ExAC gnomAD
CA1750179 rs11548928	88	L>F		No	ClinGen ESP ExAC TOPMed gnomAD
rs911237858 CA51405990	89	M>T		No	ClinGen TOPMed gnomAD
rs753416661 CA1750178	90	G>E		No	ClinGen ExAC gnomAD
rs766860986 CA1750177	93	N>Y		No	ClinGen ExAC gnomAD
rs1212501940 CA347562713	95	L>F		No	ClinGen gnomAD
rs368950086 CA1750150	96	A>V		No	ClinGen ESP ExAC gnomAD
rs775671590 CA1750147	97	V>F		No	ClinGen ExAC gnomAD
rs770016809 CA1750146	99	R>*		No	ClinGen ExAC gnomAD
rs746864912 CA1750145	99	R>Q		No	ClinGen ExAC TOPMed gnomAD
rs1204057476 CA347561254	100	V>G		No	ClinGen gnomAD
CA1750141 rs754597514	103	S>C		No	ClinGen ExAC
rs754597514 CA1750140	103	S>F		No	ClinGen ExAC
rs754597514 CA1750142	103	S>Y		No	ClinGen ExAC
rs753507067 CA1750139	104	L>M		No	ClinGen ExAC TOPMed gnomAD
CA1750138 rs779478429	104	L>P		No	ClinGen ExAC
rs753507067 CA51393858	104	L>V		No	ClinGen ExAC TOPMed gnomAD
CA1750136 rs749965778	105	Q>P		No	ClinGen ExAC TOPMed gnomAD
CA347561224 rs1481158555	106	K>N		No	ClinGen gnomAD
CA1750135 rs768053993	106	K>Q		No	ClinGen ExAC
CA347561219 rs1309107963	107	S>N		No	ClinGen TOPMed
CA347561211 rs1239426479	108	T>K		No	ClinGen gnomAD
rs1573236753 CA347561195	110	V>G		No	ClinGen Ensembl
rs757752779 CA1750134	111	M>V		No	ClinGen ExAC TOPMed gnomAD
rs151030608 CA1750133	112	K>N		No	ClinGen ESP ExAC TOPMed gnomAD
CA1750132 rs764585197	116	S>G		No	ClinGen ExAC gnomAD
CA347561153 rs1254623189	116	S>N		No	ClinGen TOPMed
rs376633964 CA1750131	118	V>M		No	ClinGen ESP ExAC gnomAD
CA347561125 rs1216872800	120	I>T		No	ClinGen gnomAD
CA347561117 rs1316876854	121	P>L		No	ClinGen gnomAD
CA347561109 rs1282713848	122	E>D		No	ClinGen gnomAD
CA347561110 rs1282713848	122	E>D		No	ClinGen gnomAD
rs1006124257 CA51393830	124	Q>*		No	ClinGen Ensembl
rs770818481 CA51393825	126	T>A		No	ClinGen gnomAD
CA1750130 rs148511443	127	M>V		No	ClinGen ESP ExAC TOPMed gnomAD
rs540945205 CA1750129	129	E>Q		No	ClinGen 1000Genomes ExAC gnomAD
CA347560924 rs1573236614	136	K>R		No	ClinGen Ensembl
rs766405014 CA1750108	140	I>V		No	ClinGen ExAC gnomAD
rs761747904 CA1750107	141	E>D		No	ClinGen ExAC gnomAD
CA347559948 rs1462177835	144	L>S		No	ClinGen gnomAD
CA1750105 rs774273747	145	E>K		No	ClinGen ExAC gnomAD
CA347559869 rs1469334620	147	T>S		No	ClinGen TOPMed
rs1481680405 CA347559841	148	F>S		No	ClinGen gnomAD
CA1750103 rs199633699	152	D>E		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs144721613 CA1750102	153	D>N		No	ClinGen ESP ExAC TOPMed gnomAD
rs1305929078 CA347559699	154	Q>R		No	ClinGen gnomAD
CA1750101 rs367837051	155	E>K		No	ClinGen ESP ExAC TOPMed gnomAD
CA1750100 rs745536967	156	E>D		No	ClinGen ExAC TOPMed gnomAD
CA347559603 rs1205641112	158	E>A		No	ClinGen gnomAD
rs1358705124 CA347559556	160	E>A		No	ClinGen TOPMed gnomAD
rs1326612180 CA347559529	162	E>K		No	ClinGen TOPMed
rs780703545 CA1750099	163	M>V		No	ClinGen ExAC gnomAD
rs912806492 CA51392344	164	E>D		No	ClinGen Ensembl
CA1750097 rs578080094	167	R>I		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA347559437 rs578080094	167	R>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA51392316 rs201616389	169	L>I		No	ClinGen Ensembl
CA347559410 rs1298238295	171	E>G		No	ClinGen gnomAD
rs553362782 CA51392314	173	T>I		No	ClinGen 1000Genomes
CA347559192 rs932718886	176	A>D		No	ClinGen TOPMed gnomAD
CA347559195 rs1328266785	176	A>S		No	ClinGen TOPMed gnomAD
CA347559199 rs1328266785	176	A>T		No	ClinGen TOPMed gnomAD
CA51391558 rs932718886	176	A>V		No	ClinGen TOPMed gnomAD
CA347559189 rs1364842745	177	L>V		No	ClinGen TOPMed gnomAD
rs1471681654 CA347559180	178	G>D		No	ClinGen TOPMed gnomAD
rs1200503151 CA347559155	182	S>G		No	ClinGen gnomAD
CA1750077 rs370256030	185	T>P		No	ClinGen ESP ExAC TOPMed gnomAD
CA1750074 rs754290630	189	P>L		No	ClinGen ExAC gnomAD
rs1573230781 CA347559085	192	E>D		No	ClinGen Ensembl
CA1750072 rs756370546	192	E>Q		No	ClinGen ExAC TOPMed gnomAD
rs372637780 CA1750071	194	P>L		No	ClinGen ESP ExAC TOPMed gnomAD
CA1750070 rs751335215	195	G>R		No	ClinGen ExAC TOPMed gnomAD
rs762838113 CA347559064	196	A>G		No	ClinGen ExAC gnomAD
rs1472946034 CA347559067	196	A>P		No	ClinGen TOPMed
rs762838113 CA1750069	196	A>V		No	ClinGen ExAC gnomAD
rs765024225 CA1750067	197	M>K		No	ClinGen ExAC TOPMed gnomAD
CA51391521 rs896090722	197	M>L		No	ClinGen Ensembl
rs1465903669 CA347559055	198	A>T		No	ClinGen TOPMed
COSM364504 rs1376898188 CA347559030	202	D>N	lung [Cosmic]	No	ClinGen cosmic curated TOPMed
rs759236870 CA1750066	203	E>K		No	ClinGen ExAC gnomAD
rs1386110607 CA347559020	203	E>V		No	ClinGen gnomAD
CA1750065 rs776553670	204	E>K		No	ClinGen ExAC gnomAD
rs770673887 CA1750064	205	E>K		No	ClinGen ExAC gnomAD
rs770673887 CA347559007	205	E>Q		No	ClinGen ExAC gnomAD
rs760450254 CA1750063	206	E>K		No	ClinGen ExAC gnomAD
rs937688635 CA347558987	207	E>D		No	ClinGen TOPMed
rs1177172351 CA347558994	207	E>K		No	ClinGen gnomAD
CA347558993 rs1177172351	207	E>Q		No	ClinGen gnomAD
rs1573230637 CA347558968	210	L>R		No	ClinGen Ensembl
rs1208087749 CA347558945	213	M>I		No	ClinGen gnomAD
rs1326914668 CA347558942	214	Q>*		No	ClinGen gnomAD
rs1326914668 CA347558944	214	Q>K		No	ClinGen gnomAD
CA1750057 rs369026092	216	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
rs748612866 CA1750058	216	R>W		No	ClinGen ExAC TOPMed gnomAD
CA1750056 rs769276177	218	A>V		No	ClinGen ExAC gnomAD
CA347558906 rs1432424342	220	L>R		No	ClinGen TOPMed gnomAD
rs1327456483 CA347558902	221	R>H		No	ClinGen gnomAD

No associated diseases with Q9Y3E7

No regional properties for Q9Y3E7

Type	Name	Position	InterPro Accession
No domain, repeats, and functional sites for Q9Y3E7

Functions

		Description
EC Number
Subcellular Localization	Cytoplasm, cytosol Membrane; Lipid-anchor Endosome Late endosome membrane	Localizes to the midbody of dividing cells
PANTHER Family	PTHR10476	CHARGED MULTIVESICULAR BODY PROTEIN
PANTHER Subfamily	PTHR10476:SF1	CHARGED MULTIVESICULAR BODY PROTEIN 3
PANTHER Protein Class	membrane traffic protein
PANTHER Pathway Category	No pathway information available

15 GO annotations of cellular component

Name	Definition
amphisome membrane	Any membrane that is part of an amphisome.
autophagosome membrane	The lipid bilayer surrounding an autophagosome, a double-membrane-bounded vesicle in which endogenous cellular material is sequestered.
cytoplasmic vesicle	A vesicle found in the cytoplasm of a cell.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
ESCRT III complex	A complex with membrane scission activity that plays a major role in many processes where membranes are remodelled - including endosomal transport (vesicle budding), nuclear envelope organisation (membrane closure, mitotic bridge cleavage), and cytokinesis (abscission).
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
kinetochore	A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
kinetochore microtubule	Any of the spindle microtubules that attach to the kinetochores of chromosomes by their plus ends, and maneuver the chromosomes during mitotic or meiotic chromosome segregation.
late endosome	A prelysosomal endocytic organelle differentiated from early endosomes by lower lumenal pH and different protein composition. Late endosomes are more spherical than early endosomes and are mostly juxtanuclear, being concentrated near the microtubule organizing center.
lysosomal membrane	The lipid bilayer surrounding the lysosome and separating its contents from the cell cytoplasm.
midbody	A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
multivesicular body	A type of endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.
multivesicular body membrane	The lipid bilayer surrounding a multivesicular body.
nuclear pore	A protein complex providing a discrete opening in the nuclear envelope of a eukaryotic cell, where the inner and outer nuclear membranes are joined.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

3 GO annotations of molecular function

Name	Definition
identical protein binding	Binding to an identical protein or proteins.
phosphatidylcholine binding	Binding to a phosphatidylcholine, a glycophospholipid in which a phosphatidyl group is esterified to the hydroxyl group of choline.
ubiquitin-specific protease binding	Binding to a ubiquitin-specific protease.

26 GO annotations of biological process

Name	Definition
apoptotic process	A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
autophagosome maturation	Removal of PI3P and Atg8/LC3 after the closure of the phagophore and before the fusion with the endosome/lysosome (e.g. mammals and insects) or vacuole (yeast), and that very likely destabilizes other Atg proteins and thus enables their efficient dissociation and recycling.
autophagy	The cellular catabolic process in which cells digest parts of their own cytoplasm; allows for both recycling of macromolecular constituents under conditions of cellular stress and remodeling the intracellular structure for cell differentiation.
endosome transport via multivesicular body sorting pathway	The directed movement of substances from endosomes to lysosomes or vacuoles by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the target compartment.
late endosome to lysosome transport	The directed movement of substances from late endosome to lysosome.
late endosome to vacuole transport	The directed movement of substances from late endosomes to the vacuole. In yeast, after transport to the prevacuolar compartment, endocytic content is delivered to the late endosome and on to the vacuole. This pathway is analogous to endosome to lysosome transport.
macroautophagy	The major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane-bounded autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane-bounded structure. Autophagosomes then fuse with a lysosome (or vacuole) releasing single-membrane-bounded autophagic bodies that are then degraded within the lysosome (or vacuole). Some types of macroautophagy, e.g. pexophagy, mitophagy, involve selective targeting of the targets to be degraded.
midbody abscission	The process by which the midbody, the cytoplasmic bridge that connects the two prospective daughter cells, is severed at the end of mitotic cytokinesis, resulting in two separate daughter cells.
mitotic metaphase plate congression	The cell cycle process in which chromosomes are aligned at the metaphase plate, a plane halfway between the poles of the mitotic spindle, during mitosis.
multivesicular body assembly	The aggregation, arrangement and bonding together of a set of components to form a multivesicular body, a type of late endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.
multivesicular body sorting pathway	A vesicle-mediated transport process in which transmembrane proteins are ubiquitylated to facilitate their entry into luminal vesicles of multivesicular bodies (MVBs); upon subsequent fusion of MVBs with lysosomes or vacuoles, the cargo proteins are degraded.
multivesicular body-lysosome fusion	The organelle membrane fusion process in which the membrane of a multivesicular body fuses with a lysosome to create a hybrid organelle.
negative regulation of cell death	Any process that decreases the rate or frequency of cell death. Cell death is the specific activation or halting of processes within a cell so that its vital functions markedly cease, rather than simply deteriorating gradually over time, which culminates in cell death.
nuclear membrane reassembly	The reformation of the nuclear membranes following their breakdown in the context of a normal process.
nucleus organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the nucleus.
plasma membrane repair	The resealing of a cell plasma membrane after cellular wounding due to, for instance, mechanical stress.
protein polymerization	The process of creating protein polymers, compounds composed of a large number of component monomers; polymeric proteins may be made up of different or identical monomers. Polymerization occurs by the addition of extra monomers to an existing poly- or oligomeric protein.
protein transport	The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of centrosome duplication	Any process that modulates the frequency, rate or extent of centrosome duplication. Centrosome duplication is the replication of a centrosome, a structure comprised of a pair of centrioles and peri-centriolar material from which a microtubule spindle apparatus is organized.
regulation of early endosome to late endosome transport	Any process that modulates the frequency, rate or extent of early endosome to late endosome transport.
regulation of mitotic spindle assembly	Any process that modulates the frequency, rate or extent of mitotic spindle assembly.
suppression of viral release by host	A process in which a host organism stops, prevents or reduces the frequency, rate or extent of the release of a virus with which it is infected, from its cells.
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	The chemical reactions and pathways resulting in the breakdown of a protein or peptide covalently tagged with ubiquitin, via the multivesicular body (MVB) sorting pathway; ubiquitin-tagged proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation.
viral budding from plasma membrane	A viral budding that starts with formation of a membrane curvature in the host plasma membrane.
viral budding via host ESCRT complex	Viral budding which uses a host ESCRT protein complex, or complexes, to mediate the budding process.
viral release from host cell	The dissemination of mature viral particles from the host cell, e.g. by cell lysis or the budding of virus particles from the cell membrane.

25 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q58CS7	CHMP3	Charged multivesicular body protein 3	Bos taurus (Bovine)	SS
Q9VE61	CG7694	E3 ubiquitin-protein ligase RNF181 homolog	Drosophila melanogaster (Fruit fly)	PR
Q9Y3E7	CHMP3	Charged multivesicular body protein 3	Homo sapiens (Human)	EV
O00237	RNF103	E3 ubiquitin-protein ligase RNF103	Homo sapiens (Human)	PR
Q86Y13	DZIP3	E3 ubiquitin-protein ligase DZIP3	Homo sapiens (Human)	PR
Q7LBR1	CHMP1B	Charged multivesicular body protein 1b	Homo sapiens (Human)	PR
O43633	CHMP2A	Charged multivesicular body protein 2a	Homo sapiens (Human)	EV
Q9R1W3	Rnf103	E3 ubiquitin-protein ligase RNF103	Mus musculus (Mouse)	PR
Q9CQ10	Chmp3	Charged multivesicular body protein 3	Mus musculus (Mouse)	SS
Q9EPZ8	Rnf103	E3 ubiquitin-protein ligase RNF103	Rattus norvegicus (Rat)	PR
Q8CGS4	Chmp3	Charged multivesicular body protein 3	Rattus norvegicus (Rat)	SS
Q9SI09	XERICO	Probable E3 ubiquitin-protein ligase XERICO	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9ZV51	ATL56	RING-H2 finger protein ATL56	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9SKK8	ATL22	RING-H2 finger protein ATL22	Arabidopsis thaliana (Mouse-ear cress)	PR
P0CH02	ATL21B	Putative RING-H2 finger protein ATL21B	Arabidopsis thaliana (Mouse-ear cress)	PR
P0CH01	ATL21A	Putative RING-H2 finger protein ATL21A	Arabidopsis thaliana (Mouse-ear cress)	PR
O22255	ATL64	RING-H2 finger protein ATL64	Arabidopsis thaliana (Mouse-ear cress)	PR
Q8RXX9	ATL6	E3 ubiquitin-protein ligase ATL6	Arabidopsis thaliana (Mouse-ear cress)	PR
Q8GYT9	SIS3	E3 ubiquitin-protein ligase SIS3	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9LZJ6	ATL5	RING-H2 finger protein ATL5	Arabidopsis thaliana (Mouse-ear cress)	PR
Q8GXF8	SGR9	E3 ubiquitin-protein ligase SGR9, amyloplastic	Arabidopsis thaliana (Mouse-ear cress)	PR
Q8LGA5	ATL31	E3 ubiquitin-protein ligase ATL31	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9LUZ9	ATL63	RING-H2 finger protein ATL63	Arabidopsis thaliana (Mouse-ear cress)	PR
Q5BKM3	chmp3	Charged multivesicular body protein 3	Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)	SS
Q6NY88	chmp3	Charged multivesicular body protein 3	Danio rerio (Zebrafish) (Brachydanio rerio)	SS

10	20	30	40	50	60
MGLFGKTQEK	PPKELVNEWS	LKIRKEMRVV	DRQIRDIQRE	EEKVKRSVKD	AAKKGQKDVC
70	80	90	100	110	120
IVLAKEMIRS	RKAVSKLYAS	KAHMNSVLMG	MKNQLAVLRV	AGSLQKSTEV	MKAMQSLVKI
130	140	150	160	170	180
PEIQATMREL	SKEMMKAGII	EEMLEDTFES	MDDQEEMEEE	AEMEIDRILF	EITAGALGKA
190	200	210	220
PSKVTDALPE	PEPPGAMAAS	EDEEEEEEAL	EAMQSRLATL	RS