Q9Y1J3

SS
Gene name
pdk-1 (H42K12.1)
Protein name
3-phosphoinositide-dependent protein kinase 1
Names
EC 2.7.11.1 , Pdk-class protein kinase 1
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_H42K12.1
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

3-Phosphoinositide-dependent protein kinase-1 (PDPK1) plays a central role in activating the protein kinase A, G, and C subfamily. In particular, PDPK1 plays an important role in regulating the Akt survival pathway. PDPK1 forms a homodimer via its PH domain, which is an autoinhibited conformation. The ATP-binding site is buried in the structure and the PIF pocket in the kinase domain of one monomer is not accessible because of its occupancy by the αG helix of the other monomer. Thus, though the PH domain in PDPK1 does not mediate intramolecular interactions, it mediates the homodimerization by PH-PH interaction and results in autoinhibition. Deletion of the PH domain increases the interaction between PDPK1 and PKB.

Autoinhibitory domains (AIDs)

482-573 (PH domain) SS

Target domain

69-364 (Protein kinase domain)

Relief mechanism

PTM, Ligand binding

Assay

Accessory elements

214-224 (Activation loop from InterPro)

Target domain

69-364 (Protein kinase domain)

Relief mechanism

Assay

267-275 (Activation loop from InterPro)

Target domain

69-364 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q9Y1J3

Entry ID Method Resolution Chain Position Source
AF-Q9Y1J3-F1 Predicted AlphaFoldDB

No variants for Q9Y1J3

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9Y1J3

No associated diseases with Q9Y1J3

4 regional properties for Q9Y1J3

Type Name Position InterPro Accession
domain Protein kinase domain 69 - 364 IPR000719
domain Pleckstrin homology domain 480 - 569 IPR001849
active_site Serine/threonine-protein kinase, active site 193 - 205 IPR008271
domain PDK1-type, PH domain 464 - 570 IPR033931

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
axon The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

8 GO annotations of biological process

Name Definition
cellular response to metal ion Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a metal ion stimulus.
cellular response to reactive oxygen species Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a reactive oxygen species stimulus. Reactive oxygen species include singlet oxygen, superoxide, and oxygen free radicals.
dauer larval development The process whose specific outcome is the progression of the dauer larva over time, through the facultative diapause of the dauer (enduring) larval stage, with specialized traits adapted for dispersal and long-term survival, with elevated stress resistance and without feeding.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
learning or memory The acquisition and processing of information and/or the storage and retrieval of this information over time.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
regulation of chemotaxis Any process that modulates the frequency, rate or extent of the directed movement of a motile cell or organism in response to a specific chemical concentration gradient.
regulation of synaptic assembly at neuromuscular junction Any process that modulates the frequency, rate or extent of synaptic assembly at neuromuscular junctions.

13 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q03407 PKH1 Serine/threonine-protein kinase PKH1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q9W0V1 Pdk1 3-phosphoinositide-dependent protein kinase 1 Drosophila melanogaster (Fruit fly) SS
O15530 PDPK1 3-phosphoinositide-dependent protein kinase 1 Homo sapiens (Human) EV
Q6A1A2 PDPK2P Putative 3-phosphoinositide-dependent protein kinase 2 Homo sapiens (Human) PR
Q9Z2A0 Pdpk1 3-phosphoinositide-dependent protein kinase 1 Mus musculus (Mouse) SS
O55173 Pdpk1 3-phosphoinositide-dependent protein kinase 1 Rattus norvegicus (Rat) SS
Q19266 pkc-3 Protein kinase C-like 3 Caenorhabditis elegans SS
P34722 tpa-1 Protein kinase C-like 1 Caenorhabditis elegans PR
P90980 pkc-2 Protein kinase C-like 2 Caenorhabditis elegans SS
Q9XTG7 akt-2 Serine/threonine-protein kinase akt-2 Caenorhabditis elegans SS
Q17941 akt-1 Serine/threonine-protein kinase akt-1 Caenorhabditis elegans PR
Q2L6W9 sax-1 Serine/threonine-protein kinase sax-1 Caenorhabditis elegans SS
Q4V3C8 PDPK2 3-phosphoinositide-dependent protein kinase 2 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MEDLTPTNTS LDTTTTNNDT TSDREAAPTT LNLTPTASES ENSLSPVTAE DLIAKSIKEG
70 80 90 100 110 120
CPKRTSNDFM FLQSMGEGAY SQVFRCREVA TDAMFAVKVL QKSYLNRHQK MDAIIREKNI
130 140 150 160 170 180
LTYLSQECGG HPFVTQLYTH FHDQARIYFV IGLVENGDLG ESLCHFGSFD MLTSKFFASE
190 200 210 220 230 240
ILTGLQFLHD NKIVHRDMKP DNVLIQKDGH ILITDFGSAQ AFGGLQLSQE GFTDANQASS
250 260 270 280 290 300
RSSDSGSPPP TRFYSDEEVP EENTARRTTF VGTALYVSPE MLADGDVGPQ TDIWGLGCIL
310 320 330 340 350 360
FQCLAGQPPF RAVNQYHLLK RIQELDFSFP EGFPEEASEI IAKILVRDPS TRITSQELMA
370 380 390 400 410 420
HKFFENVDWV NIANIKPPVL HAYIPATFGE PEYYSNIGPV EPGLDDRALF RLMNLGNDAS
430 440 450 460 470 480
ASQPSTFRPS NVEHRGDPFV SEIAPRANSE AEKNRAARAQ KLEEQRVKNP FHIFTNNSLI
490 500 510 520 530 540
LKQGYLEKKR GLFARRRMFL LTEGPHLLYI DVPNLVLKGE VPWTPCMQVE LKNSGTFFIH
550 560 570 580 590 600
TPNRVYYLFD LEKKADEWCK AINDVRKRYS VTIEKTFNSA MRDGTFGSIY GKKKSRKEMM
610 620 630
REQKALRRKQ EKEEKKALKA EQVSKKLSMQ MDKKSP