Q9WVE9

SS
Gene name
Itsn1 (Ehsh1, Itsn)
Protein name
Intersectin-1
Names
EH domain and SH3 domain regulator of endocytosis 1
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:29491
EC number
Protein Class

Descriptions

Autoinhibitory domains (AIDs)

718-737 (N-terminal linker of SH3A) SS

Target domain

738-795 (SH3A domain)

Relief mechanism

Assay

796-832 (C-terminal linker of SH3A) SS

Target domain

738-795 (SH3A domain)

Relief mechanism

PTM

Assay

1150-1202 (SH3E domain) SS

Target domain

1230-1416 (DH domain)

Relief mechanism

Partner binding, Ligand binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for Q9WVE9

Entry ID Method Resolution Chain Position Source
3HS9 X-ray 215 A P 844-855 PDB
AF-Q9WVE9-F1 Predicted AlphaFoldDB

2 variants for Q9WVE9

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3322045015 1426 E>G No EVA
rs3321999848 1427 W>G No EVA

No associated diseases with Q9WVE9

10 regional properties for Q9WVE9

Type Name Position InterPro Accession
domain SNF2, N-terminal 186 - 465 IPR000330
domain SANT/Myb domain 856 - 905 IPR001005-1
domain SANT/Myb domain 958 - 1022 IPR001005-2
domain Helicase, C-terminal domain-like 487 - 653 IPR001650
domain Helicase superfamily 1/2, ATP-binding domain 179 - 371 IPR014001
domain ISWI, HAND domain 758 - 856 IPR015194
domain SLIDE domain 913 - 1026 IPR015195
domain SANT domain 855 - 907 IPR017884
domain SMARCA1, N-terminal 168 - 400 IPR044755
domain SNF2/RAD5-like, C-terminal helicase domain 479 - 623 IPR049730

Functions

Description
EC Number
Subcellular Localization
  • Endomembrane system
  • Synapse, synaptosome
  • Cell projection, lamellipodium
  • Cell membrane
  • Membrane, clathrin-coated pit
  • Recycling endosome
  • Endosome
  • Cytoplasmic vesicle
  • Colocalizes with SGIP1 at the plasma membrane in structures corresponding most probably to clathrin-coated pits
  • Colocalizes with RAB13 on cytoplasmic vesicles that are most likely recycling endosomes
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

17 GO annotations of cellular component

Name Definition
apical dendrite A dendrite that emerges near the apical pole of a neuron. In bipolar neurons, apical dendrites are located on the opposite side of the soma from the axon.
calyx of Held The terminal specialization of a calyciferous axon which forms large synapses in the mammalian auditory central nervous system.
clathrin-coated pit A part of the endomembrane system in the form of an invagination of a membrane upon which a clathrin coat forms, and that can be converted by vesicle budding into a clathrin-coated vesicle. Coated pits form on the plasma membrane, where they are involved in receptor-mediated selective transport of many proteins and other macromolecules across the cell membrane, in the trans-Golgi network, and on some endosomes.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
dendritic spine A small, membranous protrusion from a dendrite that forms a postsynaptic compartment, typically receiving input from a single presynapse. They function as partially isolated biochemical and an electrical compartments. Spine morphology is variable:they can be thin, stubby, mushroom, or branched, with a continuum of intermediate morphologies. They typically terminate in a bulb shape, linked to the dendritic shaft by a restriction. Spine remodeling is though to be involved in synaptic plasticity.
endocytic vesicle A membrane-bounded intracellular vesicle formed by invagination of the plasma membrane around an extracellular substance. Endocytic vesicles fuse with early endosomes to deliver the cargo for further sorting.
glutamatergic synapse A synapse that uses glutamate as a neurotransmitter.
intracellular vesicle Any vesicle that is part of the intracellular region.
lamellipodium A thin sheetlike process extended by the leading edge of a migrating cell or extending cell process; contains a dense meshwork of actin filaments.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
nuclear envelope The double lipid bilayer enclosing the nucleus and separating its contents from the rest of the cytoplasm; includes the intermembrane space, a gap of width 20-40 nm (also called the perinuclear space).
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
postsynaptic actin cytoskeleton The actin cytoskeleton that is part of a postsynapse.
presynaptic endocytic zone A specialized region of the plasma membrane and underlying cytoplasm which surround the the active zone, into which synaptic vesicle membranes are recycled following exocytosis. It is especially enriched in endocytic proteins following intense activity.
presynaptic membrane A specialized area of membrane of the axon terminal that faces the plasma membrane of the neuron or muscle fiber with which the axon terminal establishes a synaptic junction; many synaptic junctions exhibit structural presynaptic characteristics, such as conical, electron-dense internal protrusions, that distinguish it from the remainder of the axon plasma membrane.
recycling endosome An organelle consisting of a network of tubules that functions in targeting molecules, such as receptors transporters and lipids, to the plasma membrane.
terminal bouton Terminal inflated portion of the axon, containing the specialized apparatus necessary to release neurotransmitters. The axon terminus is considered to be the whole region of thickening and the terminal bouton is a specialized region of it.

5 GO annotations of molecular function

Name Definition
calcium ion binding Binding to a calcium ion (Ca2+).
guanyl-nucleotide exchange factor activity Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions.
kinase activator activity Binds to and increases the activity of a kinase, an enzyme which catalyzes of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
molecular adaptor activity The binding activity of a molecule that brings together two or more molecules through a selective, non-covalent, often stoichiometric interaction, permitting those molecules to function in a coordinated way.
proline-rich region binding Binding to a proline-rich region, i.e. a region that contains a high proportion of proline residues, in a protein.

15 GO annotations of biological process

Name Definition
clathrin-dependent synaptic vesicle endocytosis Clathrin-dependent endocytosis of presynaptic membrane regions comprising synaptic vesicles' membrane constituents. This is a relatively slow process occurring in the range of tens of seconds.
endosomal transport The directed movement of substances mediated by an endosome, a membrane-bounded organelle that carries materials enclosed in the lumen or located in the endosomal membrane.
exocytosis A process of secretion by a cell that results in the release of intracellular molecules (e.g. hormones, matrix proteins) contained within a membrane-bounded vesicle. Exocytosis can occur either by full fusion, when the vesicle collapses into the plasma membrane, or by a kiss-and-run mechanism that involves the formation of a transient contact, a pore, between a granule (for exemple of chromaffin cells) and the plasma membrane. The latter process most of the time leads to only partial secretion of the granule content. Exocytosis begins with steps that prepare vesicles for fusion with the membrane (tethering and docking) and ends when molecules are secreted from the cell.
negative regulation of neuron apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process in neurons.
neuron apoptotic process Any apoptotic process in a neuron, the basic cellular unit of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the nervous system.
positive regulation of caveolin-mediated endocytosis Any process that activates or increases the frequency, rate or extent of caveolin-mediated endocytosis.
positive regulation of dendritic spine development Any process that increases the rate, frequency, or extent of dendritic spine development, the process whose specific outcome is the progression of the dendritic spine over time, from its formation to the mature structure.
positive regulation of growth hormone secretion Any process that increases the frequency, rate or extent of the regulated release of growth hormone from a cell.
positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction Any process that activates or increases the frequency, rate or extent of protein kinase B signaling, a series of reactions mediated by the intracellular serine/threonine kinase protein kinase B.
protein localization Any process in which a protein is transported to, or maintained in, a specific location.
protein transport The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of modification of postsynaptic actin cytoskeleton Any process that modulates the frequency, rate or extent of modification of postsynaptic actin cytoskeleton.
regulation of postsynapse organization Any process that modulates the physical form of a postsynapse.
small GTPase mediated signal transduction The series of molecular signals in which a small monomeric GTPase relays a signal.
synaptic vesicle endocytosis A vesicle-mediated transport process, in which the synaptic vesicle membrane constituents are retrieved from the presynaptic membrane on the axon terminal after neurotransmitter secretion by exocytosis. Synaptic vesicle endocytosis can occur via clathrin-dependent and clathrin-independent mechanisms.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
E1BKY4 ITSN1 Intersectin 1 Bos taurus (Bovine) SS
A0A2I2U1K8 ITSN1 Intersectin 1 Felis catus (Cat) (Felis silvestris catus) SS
A0A2I3T257 ITSN1 Intersectin 1 Pan troglodytes (Chimpanzee) SS
Q9NZM3 ITSN2 Intersectin-2 Homo sapiens (Human) SS
Q15811 ITSN1 Intersectin-1 Homo sapiens (Human) EV SS
Q9Z0R6 Itsn2 Intersectin-2 Mus musculus (Mouse) SS
Q9Z0R4 Itsn1 Intersectin-1 Mus musculus (Mouse) EV
10 20 30 40 50 60
MAQFPTPFGG SLDIWAITVE ERAKHDQQFQ SLKPISGFIT GDQARNFFFQ SGLPQPVLAQ
70 80 90 100 110 120
IWALADMNKD GRMDQVEFSI AMKLIKLKLQ GYQLPPALPP VMKQQPAAIS SAPAFGIGGM
130 140 150 160 170 180
AGMPPLTAVA PVPMGSIPVV GMSPPLVSSV PQAAVPPLAN GAPPVIQPLP AFAHPAATLP
190 200 210 220 230 240
KSSSFSRSGP GSQLNTKLQK AQSFDVASAP AAAEWAVPQS SRLKYRQLFN SHDKTMSGHL
250 260 270 280 290 300
TGPQARTILM QSSLPQAQLA SIWNLSDIDQ DGKLTAEEFI LAMHLIDVAM SGQPLPPVLP
310 320 330 340 350 360
PEYIPPSFRR VRSGSGMSVI SSSSADQRLP EEPSSEDEQQ VEKKLPVTFE DKKRENFERG
370 380 390 400 410 420
NLELEKRRQA LLEQQRKEQE RLAQLERAEQ ERKERERQEQ ERKRQLELEK QLEKQRELER
430 440 450 460 470 480
QREEERRKEI ERREAAKREL ERQRQLEWER NRRQELLTQR NKDQEGIVVL KARRKTLEFE
490 500 510 520 530 540
LEALNDKKHQ LEGKLQDIRC RLATQRQEIE STNKSRELRI AEITHLQQQL QESQQMLGRL
550 560 570 580 590 600
IPEKQILSDQ LKQVQQNSLH RDSLLTLKRA LEAKELARQQ LREQLDEVEK ETRSKLQEID
610 620 630 640 650 660
VFNNQLKELR EIHSKQQLQK QRSIEAERLK QKEQERKSLE LEKQKEEGQR RVQERDKQWQ
670 680 690 700 710 720
EHVQQEEQQR PRKPHEEDKL KREDSVKKKE AEERAKPEVQ DKQSRLFHPH QEPAKPAQAP
730 740 750 760 770 780
WPTTEKGPLT ISAQESAKVV YYRALYPFES RSHDEITIQP GDIVMVDESQ TGEPGWLGGE
790 800 810 820 830 840
LKGKTGWFPA NYAEKIPENE IPTPAKPVTD LTSAPAPKLA LRETPAPLPV TSSEPSTTPN
850 860 870 880 890 900
NWADFSSTWP SSTNEKPETD NWDTWAAQPS LTVPSAGQLR QRSAFTPATA TGSSPSPVLG
910 920 930 940 950 960
QGEKVEGLQA QALYPWRAKK DNHLNFNKSD VITVLEQQDM WWFGEVQGQK GWFPKSYVKL
970 980 990 1000 1010 1020
ISGPVRKSTS IDTGPTEAPS SLKRVASPAA KPAIPGEEFV AMYTYESSEH GDLTFQQGDV
1030 1040 1050 1060 1070 1080
IVVTKKDGDW WTGTVGETSG VFPSNYVRLK DSEGSGTAGK TGSLGKKPEI AQVIASYTAT
1090 1100 1110 1120 1130 1140
GPEQLTLAPG QLILIRKKNP GGWWEGELQA RGKKRQIGWF PANYVKLLSP GTSKITPTEL
1150 1160 1170 1180 1190 1200
PKTAVQPAVC QVIGMYDYTA QNDDELAFSK GQIINVLSKE DPDWWKGEVS GQVGLFPSNY
1210 1220 1230 1240 1250 1260
VKLTTDMDPS QQWCSDLHLL DMLTPTERKR QGYIHELIVT EENYVNDLQL VTEIFQKPLT
1270 1280 1290 1300 1310 1320
ESELLTEKEV AMIFVNWKEL IMCNIKLLKA LRVRKKMSGE KMPVKMIGDI LSAQLPHMQP
1330 1340 1350 1360 1370 1380
YIRFCSCQLN GAALIQQKTD EAPDFKEFVK RLAMDPRCKG MPLSSFILKP MQRVTRYPLI
1390 1400 1410 1420 1430 1440
IKNILENTPE NHPDHSHLKH ALEKAEELCS QVNEGVREKE NSDRLEWIQA HVQCEGLSEQ
1450 1460 1470 1480 1490 1500
LVFNSVTNCL GPRKFLHSGK LYKAKSNKEL YGFLFNDFLL LTQITKPLGS SSTDKVFSPK
1510 1520 1530 1540 1550 1560
SNLQYKMYKT PIFLNEVLVK LPTDPSGDEP IFHISHIDRV YTLRAESINE RTAWVQKIKA
1570 1580 1590 1600 1610 1620
ASELYIETEK KKREKAYLVR SQRATGIGRL MVNVVEGIEL KPCRSHGKSN PYCEVTMGSQ
1630 1640 1650 1660 1670 1680
CHITKTIQDT LNPKWNSNCQ FFIRDLEQEV LCITVFERDQ FSPDDFLGRT EIRVADIKKD
1690 1700 1710
QGSKGPVTKC LLLHEVPTGE IVVRLDLQLF DEP