Q9WVE8
Gene name |
Pacsin2 |
Protein name |
Protein kinase C and casein kinase substrate in neurons protein 2 |
Names |
Syndapin-2, Syndapin-II, SdpII |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:23970 |
EC number |
|
Protein Class |
PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1 (PTHR23065) |
Descriptions
PACSIN2 (Protein kinase C and casein kinase substrate in neurons protein 2) mediates the shape formation of caveolae and consists of an F-BAR domain and an SH3 domain. The membrane tubulation activity of the F-BAR domain is autoinhibited in full-length PACSIN2, and caveolin-1 directly interacts with the F-BAR domain of PACSIN2 to release its autoinhibition, allowing membrane tubulation. PACSIN2 also recruits dynamin-2 to caveolin-1 spots for caveolae endocytosis.
Autoinhibitory domains (AIDs)
Target domain |
18-275 (The F-BAR, FES-CIP4 Homology and Bin/Amphiphysin/Rvs, domain of Protein kinase C and Casein kinase Substrate in Neurons 2, PACSIN2) |
Relief mechanism |
Partner binding |
Assay |
Deletion assay, Mutagenesis experiment, Structural analysis |
Accessory elements
No accessory elements
References
- Shimada A et al. (2010) "Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II", FEBS letters, 584, 1111-8
- Senju Y et al. (2011) "Essential role of PACSIN2/syndapin-II in caveolae membrane sculpting", Journal of cell science, 124, 2032-40
- Plomann M et al. (2010) "A hinge in the distal end of the PACSIN 2 F-BAR domain may contribute to membrane-curvature sensing", Journal of molecular biology, 400, 129-36
Autoinhibited structure
Activated structure
2 structures for Q9WVE8
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 3LLL | X-ray | 330 A | A/B | 16-302 | PDB |
| AF-Q9WVE8-F1 | Predicted | AlphaFoldDB |
3 variants for Q9WVE8
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs246234022 | 84 | I>M | No | Ensembl | |
| rs215097224 | 84 | I>V | No | Ensembl | |
| rs238617837 | 273 | S>N | No | Ensembl |
No associated diseases with Q9WVE8
Functions
| Description | ||
|---|---|---|
| EC Number | ||
| Subcellular Localization |
|
|
| PANTHER Family | PTHR23065 | PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1 |
| PANTHER Subfamily | PTHR23065:SF14 | PROTEIN KINASE C AND CASEIN KINASE SUBSTRATE IN NEURONS PROTEIN 2 |
| PANTHER Protein Class |
cytoskeletal protein
actin or actin-binding cytoskeletal protein |
|
| PANTHER Pathway Category | No pathway information available | |
14 GO annotations of cellular component
| Name | Definition |
|---|---|
| caveola | A membrane raft that forms small pit, depression, or invagination that communicates with the outside of a cell and extends inward, indenting the cytoplasm and the cell membrane. Examples include flask-shaped invaginations of the plasma membrane in adipocytes associated with caveolin proteins, and minute pits or incuppings of the cell membrane formed during pinocytosis. Caveolae may be pinched off to form free vesicles within the cytoplasm. |
| cell-cell junction | A cell junction that forms a connection between two or more cells of an organism; excludes direct cytoplasmic intercellular bridges, such as ring canals in insects. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytoskeleton | A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| early endosome | A membrane-bounded organelle that receives incoming material from primary endocytic vesicles that have been generated by clathrin-dependent and clathrin-independent endocytosis; vesicles fuse with the early endosome to deliver cargo for sorting into recycling or degradation pathways. |
| endosome | A vacuole to which materials ingested by endocytosis are delivered. |
| extrinsic component of membrane | The component of a membrane consisting of gene products and protein complexes that are loosely bound to one of its surfaces, but not integrated into the hydrophobic region. |
| glutamatergic synapse | A synapse that uses glutamate as a neurotransmitter. |
| intracellular membrane-bounded organelle | Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane. |
| nuclear speck | A discrete extra-nucleolar subnuclear domain, 20-50 in number, in which splicing factors are seen to be localized by immunofluorescence microscopy. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| recycling endosome membrane | The lipid bilayer surrounding a recycling endosome. |
| ruffle membrane | The portion of the plasma membrane surrounding a ruffle. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| cytoskeletal protein binding | Binding to a protein component of a cytoskeleton (actin, microtubule, or intermediate filament cytoskeleton). |
| identical protein binding | Binding to an identical protein or proteins. |
| lipid binding | Binding to a lipid. |
| phosphatidic acid binding | Binding to phosphatidic acid, any of a class of glycerol phosphate in which both the remaining hydroxyl groups of the glycerol moiety are esterified with fatty acids. |
| phospholipid binding | Binding to a phospholipid, a class of lipids containing phosphoric acid as a mono- or diester. |
11 GO annotations of biological process
| Name | Definition |
|---|---|
| actin cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins. |
| caveola assembly | The aggregation, arrangement and bonding together of a set of components to form a caveola. A caveola is a plasma membrane raft that forms a small pit, depression, or invagination that communicates with the outside of a cell and extends inward, indenting the cytoplasm and the cell membrane. |
| caveolin-mediated endocytosis | An endocytosis process that begins when material is taken up into plasma membrane caveolae, which then pinch off to form endocytic caveolar carriers. |
| cell projection morphogenesis | The process in which the anatomical structures of a cell projection are generated and organized. |
| cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures. |
| modulation of chemical synaptic transmission | Any process that modulates the frequency or amplitude of synaptic transmission, the process of communication from a neuron to a target (neuron, muscle, or secretory cell) across a synapse. Amplitude, in this case, refers to the change in postsynaptic membrane potential due to a single instance of synaptic transmission. |
| negative regulation of endocytosis | Any process that stops, prevents, or reduces the frequency, rate or extent of endocytosis. |
| plasma membrane tubulation | A membrane tubulation process occurring in a plasma membrane. |
| protein localization to endosome | A process in which a protein is transported to, or maintained in, a location within an endosome. |
| regulation of endocytosis | Any process that modulates the frequency, rate or extent of endocytosis. |
| signal transduction | The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. |
15 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P25623 | SYP1 | Suppressor of yeast profilin deletion | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| A7MBI0 | PACSIN1 | Protein kinase C and casein kinase substrate in neurons protein 1 | Bos taurus (Bovine) | SS |
| O13154 | PACSIN2 | Protein kinase C and casein kinase substrate in neurons protein 2 | Gallus gallus (Chicken) | SS |
| Q9BY11 | PACSIN1 | Protein kinase C and casein kinase substrate in neurons protein 1 | Homo sapiens (Human) | EV |
| Q9UKS6 | PACSIN3 | Protein kinase C and casein kinase substrate in neurons protein 3 | Homo sapiens (Human) | SS |
| Q9UNF0 | PACSIN2 | Protein kinase C and casein kinase substrate in neurons protein 2 | Homo sapiens (Human) | EV |
| Q9H939 | PSTPIP2 | Proline-serine-threonine phosphatase-interacting protein 2 | Homo sapiens (Human) | PR |
| Q61644 | Pacsin1 | Protein kinase C and casein kinase substrate in neurons protein 1 | Mus musculus (Mouse) | EV |
| Q99JB8 | Pacsin3 | Protein kinase C and casein kinase II substrate protein 3 | Mus musculus (Mouse) | SS |
| P97814 | Pstpip1 | Proline-serine-threonine phosphatase-interacting protein 1 | Mus musculus (Mouse) | PR |
| Q99M15 | Pstpip2 | Proline-serine-threonine phosphatase-interacting protein 2 | Mus musculus (Mouse) | PR |
| Q60780 | Gas7 | Growth arrest-specific protein 7 | Mus musculus (Mouse) | PR |
| Q9Z0W5 | Pacsin1 | Protein kinase C and casein kinase substrate in neurons protein 1 | Rattus norvegicus (Rat) | SS |
| Q9QY17 | Pacsin2 | Protein kinase C and casein kinase substrate in neurons 2 protein | Rattus norvegicus (Rat) | SS |
| Q4V920 | pacsin1b | Protein kinase C and casein kinase substrate in neurons protein 1 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSVTYDDSVG | VEVSSDSFWE | VGNYKRTVKR | IDDGHRLCGD | LMNCLHERAR | IEKAYAQQLT |
| 70 | 80 | 90 | 100 | 110 | 120 |
| EWARRWRQLV | EKGPQYGTVE | KAWIAVMSEA | ERVSELHLEV | KASLMNEDFE | KIKNWQKEAF |
| 130 | 140 | 150 | 160 | 170 | 180 |
| HKQMMGGFKE | TKEAEDGFRK | AQKPWAKKLK | EVEAAKKAHH | TACKEEKLAI | SREANSKADP |
| 190 | 200 | 210 | 220 | 230 | 240 |
| SLNPEQLKKL | QDKIEKCKQD | VLKTKDKYEK | SLKELDQTTP | QYMENMEQVF | EQCQQFEEKR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| LRFFREVLLE | VQKHLDLSNV | ASYKTIYREL | EQSIKAADAV | EDLRWFRANH | GPGMAMNWPQ |
| 310 | 320 | 330 | 340 | 350 | 360 |
| FEEWSADLNR | TLSRREKKKA | VDGVTLTGIN | QTGDQSGQNK | PGSNLSVPSN | PAQSTQLQSS |
| 370 | 380 | 390 | 400 | 410 | 420 |
| YNPFEDEDDT | GSSISEKEDI | KAKNVSSYEK | TQTYPTDWSD | DESNNPFSST | DANGDSNPFD |
| 430 | 440 | 450 | 460 | 470 | 480 |
| EDTTSGTEVR | VRALYDYEGQ | EHDELSFKAG | DELTKIEDED | EQGWCKGRLD | SGQVGLYPAN |
| YVEAIQ |