Q9W0V1

SS
Gene name
Pdk1 (Pk61C, CG1210)
Protein name
3-phosphoinositide-dependent protein kinase 1
Names
dPDK-1 , EC 2.7.11.1 , Serine/threonine-protein kinase 61C , dSTPK61
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG1210
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

3-Phosphoinositide-dependent protein kinase-1 (PDPK1) plays a central role in activating the protein kinase A, G, and C subfamily. In particular, PDPK1 plays an important role in regulating the Akt survival pathway. PDPK1 forms a homodimer via its PH domain, which is an autoinhibited conformation. The ATP-binding site is buried in the structure and the PIF pocket in the kinase domain of one monomer is not accessible because of its occupancy by the αG helix of the other monomer. Thus, though the PH domain in PDPK1 does not mediate intramolecular interactions, it mediates the homodimerization by PH-PH interaction and results in autoinhibition. Deletion of the PH domain increases the interaction between PDPK1 and PKB.

Autoinhibitory domains (AIDs)

676-767 (PH domain) SS

Target domain

246-571 (Protein kinase domain)

Relief mechanism

PTM, Ligand binding

Assay

Accessory elements

387-397 (Activation loop from InterPro)

Target domain

246-571 (Protein kinase domain)

Relief mechanism

Assay

467-475 (Activation loop from InterPro)

Target domain

246-571 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q9W0V1

Entry ID Method Resolution Chain Position Source
AF-Q9W0V1-F1 Predicted AlphaFoldDB

No variants for Q9W0V1

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9W0V1

No associated diseases with Q9W0V1

4 regional properties for Q9W0V1

Type Name Position InterPro Accession
domain Protein kinase domain 246 - 571 IPR000719
active_site Serine/threonine-protein kinase, active site 366 - 378 IPR008271
binding_site Protein kinase, ATP binding site 252 - 275 IPR017441
domain PDK1-type, PH domain 658 - 764 IPR033931

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic side of plasma membrane The leaflet the plasma membrane that faces the cytoplasm and any proteins embedded or anchored in it or attached to its surface.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
neuromuscular junction The junction between the axon of a motor neuron and a muscle fiber. In response to the arrival of action potentials, the presynaptic button releases molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane of the muscle fiber, leading to a change in post-synaptic potential.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

5 GO annotations of molecular function

Name Definition
3-phosphoinositide-dependent protein kinase activity Phosphatidylinositol-3-phosphate-dependent catalysis of the reaction
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein kinase activator activity Binds to and increases the activity of a protein kinase, an enzyme which phosphorylates a protein.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

16 GO annotations of biological process

Name Definition
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
cell differentiation The cellular developmental process in which a relatively unspecialized cell, e.g. embryonic or regenerative cell, acquires specialized structural and/or functional features that characterize a specific cell. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state.
cell surface receptor signaling pathway The series of molecular signals initiated by activation of a receptor on the surface of a cell. The pathway begins with binding of an extracellular ligand to a cell surface receptor, or for receptors that signal in the absence of a ligand, by ligand-withdrawal or the activity of a constitutively active receptor. The pathway ends with regulation of a downstream cellular process, e.g. transcription.
insulin receptor signaling pathway The series of molecular signals generated as a consequence of the insulin receptor binding to insulin.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
negative regulation of apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process.
negative regulation of TORC1 signaling Any process that stops, prevents or reduces the frequency, rate or extent of TORC1 signaling.
peptidyl-threonine phosphorylation The phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
phosphatidylinositol 3-kinase/protein kinase B signal transduction A series of reactions, mediated by the intracellular serine/threonine kinase protein kinase B (also called AKT), which occurs as a result of a single trigger reaction or compound.
positive regulation of cell growth Any process that activates or increases the frequency, rate, extent or direction of cell growth.
positive regulation of cell size Any process that increases cell size.
positive regulation of multicellular organism growth Any process that activates or increases the frequency, rate or extent of growth of an organism to reach its usual body size.
positive regulation of organ growth Any process that activates or increases the frequency, rate or extent of growth of an organ of an organism.
protein phosphorylation The process of introducing a phosphate group on to a protein.
spermatogenesis The developmental process by which male germ line stem cells self renew or give rise to successive cell types resulting in the development of a spermatozoa.
synaptic assembly at neuromuscular junction The assembly of a synapse at a neuromuscular junction.

14 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q03407 PKH1 Serine/threonine-protein kinase PKH1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
A1Z9X0 aPKC Atypical protein kinase C Drosophila melanogaster (Fruit fly) SS
A1Z7T0 Pkn Serine/threonine-protein kinase N Drosophila melanogaster (Fruit fly) SS
P83099 Pkcdelta Putative protein kinase C delta type homolog Drosophila melanogaster (Fruit fly) PR
P13677 inaC Protein kinase C, eye isozyme Drosophila melanogaster (Fruit fly) SS
P05130 Pkc53E Protein kinase C, brain isozyme Drosophila melanogaster (Fruit fly) SS
Q8INB9 Akt RAC serine/threonine-protein kinase Drosophila melanogaster (Fruit fly) SS
Q9NBK5 trc Serine/threonine-protein kinase tricornered Drosophila melanogaster (Fruit fly) SS
O15530 PDPK1 3-phosphoinositide-dependent protein kinase 1 Homo sapiens (Human) EV
Q6A1A2 PDPK2P Putative 3-phosphoinositide-dependent protein kinase 2 Homo sapiens (Human) PR
Q9Z2A0 Pdpk1 3-phosphoinositide-dependent protein kinase 1 Mus musculus (Mouse) SS
O55173 Pdpk1 3-phosphoinositide-dependent protein kinase 1 Rattus norvegicus (Rat) SS
Q9Y1J3 pdk-1 3-phosphoinositide-dependent protein kinase 1 Caenorhabditis elegans SS
Q4V3C8 PDPK2 3-phosphoinositide-dependent protein kinase 2 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MKCKSWSNKI NNYVVRKIKS IKINGTQQQL QLPGSGASGI AAAAVITVAS DCGENCSSNG
70 80 90 100 110 120
TEHQQHFNIA TTTATSATEA TMPAMAKEKA SATVSLGESN FRDINLKDLA VVVEAASRLH
130 140 150 160 170 180
HQQNVCGCGA VSSTENNNNS RYGSSKYLTN GHTSPLAAAV ASNSSSVATT PHCRMLHNCS
190 200 210 220 230 240
LQQYQNDIRQ QTEILDMLRQ QHQQGYQSQQ QQQQPQQQQE QQQQQEQSQQ QQQLQNPAPR
250 260 270 280 290 300
RSPNDFIFGR YIGEGSYSIV YLAVDIHSRR EYAIKVCEKR LILRERKQDY IKREREVMHQ
310 320 330 340 350 360
MTNVPGFVNL SCTFQDQRSL YFVMTYARKG DMLPYINRVG SFDVACTRHY AAELLLACEH
370 380 390 400 410 420
MHRRNVVHRD LKPENILLDE DMHTLIADFG SAKVMTAHER ALATEHCSEQ RRSNSDEDDE
430 440 450 460 470 480
DSDRLENEDE DFYDRDSEEL DDGDDEQQQE EMDSPRHRQR RYNRHRKASF VGTAQYVSPE
490 500 510 520 530 540
VLQNGPITPA ADLWALGCIV YQMIAGLPPF RGSNDYVIFK EILDCAVDFP QGFDKDAEDL
550 560 570 580 590 600
VRKLLRVDPR DRLGAQDEFG YYESIRAHPF FAGIDWQTLR QQTPPPIYPY LPGVSQDEDF
610 620 630 640 650 660
RSSYTVPGDL EPGLDERQIS RLLSAELGVG SSVAMPVKRS TAKNSFDLND AEKLQRLEQQ
670 680 690 700 710 720
KTDKWHVFAD GEVILKKGFV NKRKGLFARK RMLLLTTGPR LIYIDPVQMI KKGEIPWSPD
730 740 750 760 770 780
LRAEYKNFKI FFVHTPNRTY YLDDPEGYAI HWSEAIENMR KLAYGDPSST SAVSCSSGSS
790 800 810 820 830
NSLAVISNSS AASSSNSPTV KRSSPVNAPQ ASTASDNRTL GSTRTGTSPS KKTASK