Q9VT57

PR
Gene name
Cdk8 (CG10572)
Protein name
Cyclin-dependent kinase 8
Names
Cell division protein kinase 8, DmCdk8, Mediator complex subunit Cdk8, Mediator of RNA polymerase II transcription subunit Cdk8
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG10572
EC number
2.7.11.22: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

317-454 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

172-198 (Activation loop from InterPro)

Target domain

21-335 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q9VT57

Entry ID Method Resolution Chain Position Source
AF-Q9VT57-F1 Predicted AlphaFoldDB

No variants for Q9VT57

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9VT57

No associated diseases with Q9VT57

3 regional properties for Q9VT57

Type Name Position InterPro Accession
domain Protein kinase domain 21 - 335 IPR000719
active_site Serine/threonine-protein kinase, active site 147 - 159 IPR008271
binding_site Protein kinase, ATP binding site 27 - 52 IPR017441

Functions

Description
EC Number 2.7.11.22 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
mediator complex A protein complex that interacts with the carboxy-terminal domain of the largest subunit of RNA polymerase II and plays an active role in transducing the signal from a transcription factor to the transcriptional machinery. The mediator complex is required for activation of transcription of most protein-coding genes, but can also act as a transcriptional corepressor. The Saccharomyces complex contains several identifiable subcomplexes: a head domain comprising Srb2, -4, and -5, Med6, -8, and -11, and Rox3 proteins; a middle domain comprising Med1, -4, and -7, Nut1 and -2, Cse2, Rgr1, Soh1, and Srb7 proteins; a tail consisting of Gal11p, Med2p, Pgd1p, and Sin4p; and a regulatory subcomplex comprising Ssn2, -3, and -8, and Srb8 proteins. Metazoan mediator complexes have similar modular structures and include homologs of yeast Srb and Med proteins.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cyclin-dependent protein serine/threonine kinase activity Cyclin-dependent catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
kinase activity Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
RNA polymerase II CTD heptapeptide repeat kinase activity Catalysis of the reaction: ATP + RNA polymerase II large subunit CTD heptapeptide repeat (YSPTSPS) = ADP + H+ + phosphorylated RNA polymerase II.

7 GO annotations of biological process

Name Definition
chaeta development The process whose specific outcome is the progression of a chaeta over time, from its formation to the mature structure. A chaeta is a sensory multicellular cuticular outgrowth of a specifically differentiated cell.
G1/S transition of mitotic cell cycle The mitotic cell cycle transition by which a cell in G1 commits to S phase. The process begins with the build up of G1 cyclin-dependent kinase (G1 CDK), resulting in the activation of transcription of G1 cyclins. The process ends with the positive feedback of the G1 cyclins on the G1 CDK which commits the cell to S phase, in which DNA replication is initiated.
imaginal disc-derived leg segmentation Division of an imaginal disc-derived leg into a series of semi-repetitive parts or segments. The Drosophila leg, for example, has nine segments, each separated from the next by a flexible joint.
positive regulation of autophagy Any process that activates, maintains or increases the rate of autophagy. Autophagy is the process in which cells digest parts of their own cytoplasm.
protein phosphorylation The process of introducing a phosphate group on to a protein.
sex comb development The process whose specific outcome is the progression of the sex comb over time, from its formation to the mature structure. The sex combs are the male specific chaetae located on the prothoracic tarsal segment of the prothoracic leg.
snRNA 3'-end processing Any process involved in forming the mature 3' end of an snRNA molecule.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P39073 SSN3 Meiotic mRNA stability protein kinase SSN3 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q9BWU1 CDK19 Cyclin-dependent kinase 19 Homo sapiens (Human) PR
P49336 CDK8 Cyclin-dependent kinase 8 Homo sapiens (Human) PR
Q8BWD8 Cdk19 Cyclin-dependent kinase 19 Mus musculus (Mouse) PR
Q8R3L8 Cdk8 Cyclin-dependent kinase 8 Mus musculus (Mouse) PR
Q6P3N6 cdk8 Cyclin-dependent kinase 8 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
Q8JH47 cdk8 Cyclin-dependent kinase 8 Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MDYDFKMKTQ IERTKVEDLF NYEGCKVGRG TYGHVYKAKW KETSDGKEYA LKQIDGTGLS
70 80 90 100 110 120
MSACREIALL RELKHQNVIT LIRVFLSHND RKVFLLIDYA EHDLWHIIKF HRAAKATKKQ
130 140 150 160 170 180
VVVPRGMVKS LLYQILDGIH YLHSNWVLHR DLKPANILVM GDGNERGRVK IADMGFARLF
190 200 210 220 230 240
NAPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED
250 260 270 280 290 300
IKTSNPYHHD QLDRIFNVMG FPQDKDWEDI KKMPEHHTLT KDFKRSTYST CSLAKYMERH
310 320 330 340 350 360
KIKPDSKAFH LLQKLLLMDP NKRITSEQAM QDQYFQEEPQ PTQDVFAGCP IPYPKREFLT
370 380 390 400 410 420
DDDQEDKSDN KRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ MNAEPNAKRV RLSGAGNQQD
430 440 450
FHHQQQQQQQ QQQQQQQQQQ QMMFNQQQNF QRFN