Q9VJE5

SS
Gene name
CLIP-190 (CG5020)
Protein name
Restin homolog
Names
Cytoplasmic linker protein 190 , Microtubule-binding protein 190 , d-CLIP-190
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG5020
EC number
Protein Class

Descriptions

Cytoplasmic linker protein (CLIP-170 or CLIP1) is a prototype of the plus end-tracking proteins that regulate microtubule dynamics. The CAP-Gly-2 domain possesses a basic groove and directly binds the EExEEY/F motif of the C-terminal acidic-tail ends of α-tubulin. The C-terminal zinc knuckle domains of CLIP-170 bind the basic groove to inhibit the binding to the acidic tails of α-tubulin. Once CLIP-170 binds microtubule, the released zinc knuckle domain may serve to recruit dynein to the plus end by interacting with the dynactin subunit p150Glued and LIS1.

Autoinhibitory domains (AIDs)

1621-1684 (C-terminal zinc knuckle domains) SS

Target domain

125-307 (CAP-Gly domain)

Relief mechanism

Others

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9VJE5

Entry ID Method Resolution Chain Position Source
AF-Q9VJE5-F1 Predicted AlphaFoldDB

No variants for Q9VJE5

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9VJE5

No associated diseases with Q9VJE5

4 regional properties for Q9VJE5

Type Name Position InterPro Accession
domain CAP Gly-rich domain 125 - 190 IPR000938-1
domain CAP Gly-rich domain 239 - 307 IPR000938-2
domain CLIP1, zinc knuckle 1621 - 1638 IPR032108-1
domain CLIP1, zinc knuckle 1668 - 1684 IPR032108-2

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Cytoplasm, cytoskeleton
  • Golgi apparatus
  • Cytoplasm, cytoskeleton, microtubule organizing center
  • Cytoplasm, perinuclear region
  • Microtubule-associated (PubMed:9472041)
  • Lva-CLIP-190 complexes are found at the Golgi (PubMed:11076973)
  • In the fat body, localizes to a perinuclear non-centrosomal microtubule-organizing centers (ncMTOCs) (PubMed:32066907)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

12 GO annotations of cellular component

Name Definition
axon The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter.
axonal growth cone The migrating motile tip of a growing nerve cell axon.
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
fusome A large intracellular spectrin-rich structure that has been found in insect germline cells and mammalian hematopoietic cells. The fusome is an elongated, branched structure, formed from the spherical spectrosome organelle.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
microtubule associated complex Any multimeric complex connected to a microtubule.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
microtubule plus-end The growing (plus) end of a microtubule. In vitro, microtubules polymerize more quickly at the plus end than at the minus end. In vivo, microtubule growth occurs only at the plus end, and the plus end switches between periods of growth and shortening, a behavior known as dynamic instability.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
spindle microtubule Any microtubule that is part of a mitotic or meiotic spindle; anchored at one spindle pole.

4 GO annotations of molecular function

Name Definition
actin binding Binding to monomeric or multimeric forms of actin, including actin filaments.
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule plus-end binding Binding to the plus end of a microtubule.
myosin VI heavy chain binding Binding to a heavy chain of a myosin VI complex.

2 GO annotations of biological process

Name Definition
cellularization The separation of a multi-nucleate cell or syncytium into individual cells. An example of this is found in Drosophila melanogaster embryo development.
cytoplasmic microtubule organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of structures formed of microtubules and associated proteins in the cytoplasm of a cell.

4 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
O42184 CLIP1 CAP-Gly domain-containing linker protein 1 Gallus gallus (Chicken) SS
P30622 CLIP1 CAP-Gly domain-containing linker protein 1 Homo sapiens (Human) EV
Q922J3 Clip1 CAP-Gly domain-containing linker protein 1 Mus musculus (Mouse) SS
Q9JK25 Clip1 CAP-Gly domain-containing linker protein 1 Rattus norvegicus (Rat) EV
10 20 30 40 50 60
MSDDTSASGG TSAPFPSPVT ADPEPGATAS KLPGPIRSNI PTPATSGTGI PQPSKMKAPS
70 80 90 100 110 120
SFGSTGSVSK IGRPCCNHTT PKSGPPPREA TSMSRESDDN LSSINSAYTD NSSAVLTANT
130 140 150 160 170 180
EQFIIGQRVW LGGTRPGQIA FIGDTHFAAG EWAGVVLDEP NGKNDGCVSG KRYFQCEPKR
190 200 210 220 230 240
GIFSRLTRLT TYPLAGAQTP TSPLAKSSPD RSRTVSPTAS IRSSMLRSPG IGGKNGMAVG
250 260 270 280 290 300
DRVIVSSGFG SRPGILRYLG ETQFAPGNWC GVELDEPSGK NDGTVDDIRY FECKPKYGVF
310 320 330 340 350 360
VPIAKVSLSP SSKKTRLSRT GSRESLTSIG TMNSIATTAT SRMRMNAQQR KSSTPVKPIL
370 380 390 400 410 420
ATPKSQFSMQ DLLREKQQHV EKLMVERDLD REDAQNQALQ LQKNINELKA RIVELESALD
430 440 450 460 470 480
NERKKTEELQ CSIDEAQFCG DELNAQSQVY KEKIHDLESK ITKLVSATPS LQSILPPDLP
490 500 510 520 530 540
SDDGALQEEI AKLQEKMTIQ QKEVESRIAE QLEEEQRLRE NVKYLNEQIA TLQSELVSKD
550 560 570 580 590 600
EALEKFSLSE CGIENLRREL ELLKEENEKQ AQEAQAEFTR KLAEKSVEVL RLSSELQNLK
610 620 630 640 650 660
ATSDSLESER VNKTDECEIL QTEVRMRDEQ IRELNQQLDE VTTQLNVQKA DSSALDDMLR
670 680 690 700 710 720
LQKEGTEEKS TLLEKTEKEL VQSKEQAAKT LNDKEQLEKQ ISDLKQLAEQ EKLVREMTEN
730 740 750 760 770 780
AINQIQLEKE SIEQQLALKQ NELEDFQKKQ SESEVHLQEI KAQNTQKDFE LVESGESLKK
790 800 810 820 830 840
LQQQLEQKTL GHEKLQAALE ELKKEKETII KEKEQELQQL QSKSAESESA LKVVQVQLEQ
850 860 870 880 890 900
LQQQAAASGE EGSKTVAKLH DEISQLKSQA EETQSELKST QSNLEAKSKQ LEAANGSLEE
910 920 930 940 950 960
EAKKSGHLLE QITKLKSEVG ETQAALSSCH TDVESKTKQL EAANAALEKV NKEYAESRAE
970 980 990 1000 1010 1020
ASDLQDKVKE ITDTLHAELQ AERSSSSALH TKLSKFSDEI ATGHKELTSK ADAWSQEMLQ
1030 1040 1050 1060 1070 1080
KEKELQELRQ QLQDSQDSQT KLKAEGERKE KSFEESIKNL QEEVTKAKTE NLELSTGTQT
1090 1100 1110 1120 1130 1140
TIKDLQERLE ITNAELQHKE KMASEDAQKI ADLKTLVEAI QVANANISAT NAELSTVLEV
1150 1160 1170 1180 1190 1200
LQAEKSETNH IFELFEMEAD MNSERLIEKV TGIKEELKET HLQLDERQKK FEELEEKLKQ
1210 1220 1230 1240 1250 1260
AQQSEQKLQQ ESQTSKEKLT EIQQSLQELQ DSVKQKEELV QNLEEKVRES SSIIEAQNTK
1270 1280 1290 1300 1310 1320
LNESNVQLEN KTSCLKETQD QLLESQKKEK QLQEEAAKLS GELQQVQEAN GDIKDSLVKV
1330 1340 1350 1360 1370 1380
EELVKVLEEK LQAATSQLDA QQATNKELQE LLVKSQENEG NLQGESLAVT EKLQQLEQAN
1390 1400 1410 1420 1430 1440
GELKEALCQK ENGLKELQGK LDESNTVLES QKKSHNEIQD KLEQAQQKER TLQEETSKLA
1450 1460 1470 1480 1490 1500
EQLSQLKQAN EELQKSLQQK QLLLEKGNEF DTQLAEYQKV IDEMDDAASV KSALLEQLQN
1510 1520 1530 1540 1550 1560
RVAELETALR QANDAQKTAY LETKELRRQL ESLELEKSRE VLSLKAQMNG ASSRSGKGDE
1570 1580 1590 1600 1610 1620
VESLDIETSL AKINFLNSII ADMQQKNDAL KAKVQTLETL PMDFTKPHAF DALTKRKPAP
1630 1640 1650 1660 1670 1680
RLFCDICDEF DQHDTEDCPI QGSEDQDYST PSSESNNNEK ERKLPAPRKY CDSCEVFGHD
TSECADDETY