Q9UPY8

SS
Gene name
MAPRE3
Protein name
Microtubule-associated protein RP/EB family member 3
Names
EB1 protein family member 3 , EBF3 , End-binding protein 3 , EB3 , RP3
Species
Homo sapiens (Human)
KEGG Pathway
hsa:22924
EC number
Protein Class

Descriptions

EB1 including a Calponin homology (CH) domain and the C-terminal dimerization domain is a plus-end tracking protein that binds to the plus-end of microtubules (MT) and regulates the dynamics of the microtubule cytoskeleton. The CH domain is responsible for MT-binding and inhibited by the binding of the CH domain and the central region of the C-terminal domain including the FYF motif. SxIP competes with the CH domain and binds on the FYF motif of the C-terminal domain, and activates the CH domain.

Autoinhibitory domains (AIDs)

258-281 (EB1 autoinhibitory tail) SS

Target domain

1-130 (CH domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

8 structures for Q9UPY8

Entry ID Method Resolution Chain Position Source
1WYO NMR - A 1-146 PDB
3CO1 X-ray 140 A A 1-130 PDB
3JAK EM 350 A M/N 1-200 PDB
3JAL EM 350 A M/N 1-200 PDB
3JAR EM 350 A M/N 1-200 PDB
3TQ7 X-ray 230 A B 200-281 PDB
7SJ9 EM 380 A M/N 1-281 PDB
AF-Q9UPY8-F1 Predicted AlphaFoldDB

186 variants for Q9UPY8

Variant ID(s) Position Change Description Diseaes Association Provenance
COSM1494928 2 A>T Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs746360555 3 V>I No ExAC
TOPMed
gnomAD
rs112647020 5 V>A No Ensembl
rs1667123662 5 V>M No Ensembl
TCGA novel 6 Y>C Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1667123838 8 T>P No TOPMed
rs780532608 12 S>G No ExAC
gnomAD
rs1058547 13 E>G No Ensembl
rs1058547 13 E>V No Ensembl
rs1480315869 17 R>C No gnomAD
rs1175991309 18 H>R Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
gnomAD
rs1667124477 20 M>I No gnomAD
rs1292949697 20 M>V No gnomAD
TCGA novel 23 W>* Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs1253236181 24 V>I No gnomAD
rs768144243 26 D>N Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No ExAC
NCI-TCGA
gnomAD
TCGA novel 28 L>M Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs151223078 31 N>T No ESP
ExAC
TOPMed
gnomAD
rs747545412 32 Y>C No ExAC
TOPMed
gnomAD
rs1343200551 32 Y>H No TOPMed
rs1667125310 33 T>A No Ensembl
rs936126887 35 I>T No TOPMed
COSM291041 40 S>= Variant assessed as Somatic; LOW impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1667151970 42 A>S No Ensembl
rs1335938741 43 A>D No gnomAD
rs376817083 43 A>T No ESP
ExAC
TOPMed
gnomAD
rs1403639208 46 Q>* No gnomAD
rs987173228 49 D>G No TOPMed
gnomAD
rs1454339408 50 M>V No gnomAD
rs1361502324 53 P>L No gnomAD
rs145294178 54 G>R No ESP
ExAC
TOPMed
gnomAD
rs145294178 54 G>S No ESP
ExAC
TOPMed
gnomAD
TCGA novel 55 C>W Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs972575130 57 H>L No TOPMed
rs1203593461 57 H>Y No TOPMed
gnomAD
COSM1019460 63 F>V Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1237736375 66 K>R No gnomAD
rs1667153635 68 E>G No TOPMed
rs1175651354 73 H>Q No gnomAD
rs762060504 73 H>Y No ExAC
TOPMed
gnomAD
TCGA novel 74 N>K Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1229397083 75 F>C No TOPMed
rs1427054781 75 F>L No gnomAD
rs1334524461 83 K>R No TOPMed
gnomAD
rs1344894599 86 G>C No TOPMed
gnomAD
rs146112641 86 G>D No 1000Genomes
rs1667154601 87 V>A No TOPMed
rs1192636314 92 P>S No gnomAD
rs1346532640 97 V>E No TOPMed
COSM1407327 98 K>E Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1429139201 100 K>R No gnomAD
rs1667178236 106 E>* No Ensembl
rs1408942151 107 F>C No gnomAD
rs373095267
COSM1019461
 114 F>L Variant assessed as Somatic; MODERATE impact. large_intestine endometrium [NCI-TCGA, Cosmic] No NCI-TCGA Cosmic
cosmic curated
ESP
ExAC
NCI-TCGA
TOPMed
gnomAD
rs780104460 117 A>T No ExAC
TOPMed
gnomAD
rs746905944 119 Y>C No ExAC
gnomAD
rs201632549 119 Y>H No 1000Genomes
rs1337816916 123 D>N No gnomAD
TCGA novel
COSM1407328
 125 N>S Variant assessed as Somatic; MODERATE impact. Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA Cosmic
NCI-TCGA
COSM574793
rs1401250016
 127 L>V lung Variant assessed as Somatic; MODERATE impact. [Cosmic, NCI-TCGA] No cosmic curated
NCI-TCGA
gnomAD
rs1667179104 129 A>T No TOPMed
rs377561900 129 A>V No ESP
ExAC
TOPMed
gnomAD
rs1230874000 130 R>G No gnomAD
rs749554114
COSM1019462
 130 R>Q Variant assessed as Somatic; MODERATE impact. endometrium [NCI-TCGA, Cosmic] No NCI-TCGA Cosmic
cosmic curated
ExAC
NCI-TCGA
TOPMed
gnomAD
COSM3580473
rs1230874000
 130 R>W Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
NCI-TCGA
gnomAD
TCGA novel 132 G>A Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs774310199 132 G>S No ExAC
gnomAD
rs1667179714 134 D>H No Ensembl
rs776126217 135 V>I No ExAC
gnomAD
rs761152460 136 A>V Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No ExAC
NCI-TCGA
TOPMed
gnomAD
rs1667180305 137 P>S No TOPMed
gnomAD
COSM442726 138 P>A Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs761464811 138 P>L No ExAC
TOPMed
gnomAD
rs1378583508 138 P>S No gnomAD
rs1667180603 139 P>T No Ensembl
rs1572775500 140 N>T No Ensembl
rs1157660970 142 G>D No TOPMed
gnomAD
rs764874678 145 I>N No ExAC
gnomAD
rs1667181172 147 N>S No Ensembl
rs2148229822 151 K>N No Ensembl
TCGA novel 151 K>N Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs750037330 152 L>P No ExAC
gnomAD
rs757984031 153 I>T No ExAC
TOPMed
gnomAD
rs138427247 154 G>S No ESP
ExAC
TOPMed
gnomAD
rs754926439 155 T>I No ExAC
gnomAD
rs865942631 157 V>I No Ensembl
rs1421594932 158 P>L No gnomAD
rs1667220469 159 Q>R No gnomAD
rs919202118 161 T>A No Ensembl
rs140237898 161 T>M No ESP
ExAC
TOPMed
gnomAD
rs774010624 162 S>P No ExAC
TOPMed
gnomAD
TCGA novel 165 G>D Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs975397856 168 N>H No Ensembl
rs984651965 168 N>I No TOPMed
TCGA novel 168 N>T Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs910285704 169 M>T No TOPMed
gnomAD
rs188015519 170 Q>K No 1000Genomes
rs1343140107 171 T>I No TOPMed
rs556886277 173 G>S No ExAC
gnomAD
rs752718112 174 R>Q No ExAC
TOPMed
gnomAD
rs1230161170 174 R>W Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
gnomAD
rs144585951 177 N>K No ESP
ExAC
gnomAD
rs1667221663 177 N>Y No Ensembl
rs943139821 178 V>M Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No Ensembl
NCI-TCGA
TCGA novel 179 A>P Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
COSM1407329
rs763967601
 179 A>P large_intestine [Cosmic] No cosmic curated
ExAC
TOPMed
gnomAD
rs751778500 180 P>A No ExAC
TOPMed
gnomAD
rs1165501291 180 P>H No Ensembl
rs751778500 180 P>T No ExAC
TOPMed
gnomAD
rs755091160 181 P>A No ExAC
TOPMed
gnomAD
rs781227628 181 P>H No ExAC
TOPMed
gnomAD
TCGA novel 181 P>L Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs781227628 181 P>L No ExAC
TOPMed
gnomAD
rs781227628 181 P>R No ExAC
TOPMed
gnomAD
rs755091160 181 P>S No ExAC
TOPMed
gnomAD
rs755091160 181 P>T No ExAC
TOPMed
gnomAD
COSM1407330 182 C>A Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs777328830
COSM112113
 182 C>L Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA Cosmic
NCI-TCGA
rs1215345168 182 C>S No Ensembl
TCGA novel 184 L>I Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs945064428 185 R>Q No TOPMed
gnomAD
rs1482064737 185 R>W No gnomAD
rs1667223223 186 K>Q No Ensembl
rs1286135753 188 P>S No TOPMed
rs1286135753 188 P>T No TOPMed
rs1413894866 189 P>Q No gnomAD
rs1043008915 190 S>L No TOPMed
gnomAD
rs376613062 191 A>P No ESP
ExAC
gnomAD
COSM5396775
rs771311276
 192 R>Q Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
ExAC
NCI-TCGA
TOPMed
gnomAD
rs1667224281 194 G>S No Ensembl
rs151280427 195 G>S No ESP
ExAC
TOPMed
gnomAD
rs1572776956 196 H>P No Ensembl
rs1572776956 196 H>R No Ensembl
rs1667224981 197 E>G No Ensembl
rs1274234223 202 I>L No gnomAD
rs1667225274 203 L>I No gnomAD
rs1287734269 204 E>K No TOPMed
rs1319171778 207 Q>R No TOPMed
gnomAD
rs776475662 210 V>M No ExAC
gnomAD
rs1405825332 211 D>G No TOPMed
gnomAD
rs1405825332 211 D>V No TOPMed
gnomAD
rs1667230274 213 K>Q No Ensembl
COSM3839235 215 T>I Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs14628 216 V>L No gnomAD
rs14628 216 V>M No gnomAD
rs765039617 217 D>N No ExAC
gnomAD
rs1572777214 220 E>G No Ensembl
rs1017481777 223 R>C No Ensembl
COSM1407332
rs766068888
 223 R>H Variant assessed as Somatic; MODERATE impact. large_intestine [NCI-TCGA, Cosmic] No NCI-TCGA Cosmic
cosmic curated
Ensembl
NCI-TCGA
rs766068888 223 R>L No Ensembl
rs1667230972 229 K>Q No TOPMed
gnomAD
TCGA novel
rs1667231104
 231 R>C Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
Ensembl
rs1191523736 231 R>H No gnomAD
rs1438049103 234 E>K No gnomAD
rs1160613040 236 I>L No gnomAD
COSM1306654 236 I>M Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1160613040 236 I>V No gnomAD
rs756212692 237 C>F No ExAC
gnomAD
rs747380208 239 E>D No gnomAD
rs1429195522 239 E>Q No TOPMed
gnomAD
rs751282252 242 S>C No gnomAD
rs751282252 242 S>G No gnomAD
rs1392031004 243 E>K No TOPMed
gnomAD
rs1181996605 244 N>H No gnomAD
rs754348467 245 S>T No ExAC
gnomAD
rs757786832 246 P>S No ExAC
gnomAD
rs377651319 249 S>L No Ensembl
rs376653902 252 I>T No ESP
ExAC
TOPMed
gnomAD
COSM184276
rs1027026616
 263 A>T Variant assessed as Somatic; MODERATE impact. large_intestine [NCI-TCGA, Cosmic] No cosmic curated
NCI-TCGA
TOPMed
rs765906655 263 A>V No ExAC
TOPMed
gnomAD
rs750965193 266 E>K No ExAC
gnomAD
rs1667241093 268 D>G No Ensembl
rs780447671 268 D>N No ExAC
TOPMed
gnomAD
rs1667241180 269 E>G No TOPMed
rs1667241367 270 I>T No Ensembl
rs370257698 272 E>K No ESP
ExAC
TOPMed
gnomAD
rs370257698 272 E>Q No ESP
ExAC
TOPMed
gnomAD
rs1342815990 274 Q>K No gnomAD
rs1198147907 275 Q>E No TOPMed
gnomAD
rs780573850 275 Q>H No ExAC
gnomAD
rs1667242331 276 E>K No TOPMed
gnomAD
rs1667242419 278 Q>R No TOPMed
rs149721958 280 E>D No ESP
rs747598291 280 E>K No ExAC
TOPMed
gnomAD
rs769280062 280 E>V No ExAC
TOPMed
gnomAD
rs1667242903 281 Y>* No gnomAD

No associated diseases with Q9UPY8

2 regional properties for Q9UPY8

Type Name Position InterPro Accession
domain Calponin homology domain 14 - 118 IPR001715
domain EB1, C-terminal 194 - 264 IPR004953

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton
  • Associated with the microtubule network
  • Detected at the plus end of microtubules
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

8 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
microtubule cytoskeleton The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
microtubule plus-end The growing (plus) end of a microtubule. In vitro, microtubules polymerize more quickly at the plus end than at the minus end. In vivo, microtubule growth occurs only at the plus end, and the plus end switches between periods of growth and shortening, a behavior known as dynamic instability.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
mitotic spindle astral microtubule end Any microtubule end that is part of a mitotic spindle astral microtubule.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
spindle midzone The area in the center of the spindle where the spindle microtubules from opposite poles overlap.

4 GO annotations of molecular function

Name Definition
identical protein binding Binding to an identical protein or proteins.
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule plus-end binding Binding to the plus end of a microtubule.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.

9 GO annotations of biological process

Name Definition
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
positive regulation of cyclin-dependent protein serine/threonine kinase activity Any process that activates or increases the frequency, rate or extent of CDK activity.
positive regulation of DNA-templated transcription Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription.
positive regulation of protein kinase activity Any process that activates or increases the frequency, rate or extent of protein kinase activity.
protein localization Any process in which a protein is transported to, or maintained in, a specific location.
protein localization to microtubule A process in which a protein is transported to, or maintained at, a microtubule.
regulation of microtubule polymerization Any process that modulates the frequency, rate or extent of microtubule polymerization.
regulation of microtubule polymerization or depolymerization Any process that modulates the frequency, rate or extent of microtubule polymerization or depolymerization by the addition or removal of tubulin heterodimers from a microtubule.
spindle assembly The aggregation, arrangement and bonding together of a set of components to form the spindle, the array of microtubules and associated molecules that serves to move duplicated chromosomes apart.

12 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q3ZBD9 MAPRE1 Microtubule-associated protein RP/EB family member 1 Bos taurus (Bovine) SS
Q5ZLC7 MAPRE1 Microtubule-associated protein RP/EB family member 1 Gallus gallus (Chicken) SS
Q15555 MAPRE2 Microtubule-associated protein RP/EB family member 2 Homo sapiens (Human) PR
Q15691 MAPRE1 Microtubule-associated protein RP/EB family member 1 Homo sapiens (Human) EV
Q61166 Mapre1 Microtubule-associated protein RP/EB family member 1 Mus musculus (Mouse) SS
Q8R001 Mapre2 Microtubule-associated protein RP/EB family member 2 Mus musculus (Mouse) PR
Q6PER3 Mapre3 Microtubule-associated protein RP/EB family member 3 Mus musculus (Mouse) SS
Q66HR2 Mapre1 Microtubule-associated protein RP/EB family member 1 Rattus norvegicus (Rat) SS
Q5XIT1 Mapre3 Microtubule-associated protein RP/EB family member 3 Rattus norvegicus (Rat) SS
Q9FJJ5 EB1B Microtubule-associated protein RP/EB family member 1B Arabidopsis thaliana (Mouse-ear cress) EV
Q7XJ60 EB1A Microtubule-associated protein RP/EB family member 1A Arabidopsis thaliana (Mouse-ear cress) SS
Q6P848 mapre1 Microtubule-associated protein RP/EB family member 1 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
10 20 30 40 50 60
MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM LFPGCVHLRK
70 80 90 100 110 120
VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK FQDNFEFIQW FKKFFDANYD
130 140 150 160 170 180
GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP
190 200 210 220 230 240
PCILRKNPPS ARNGGHETDA QILELNQQLV DLKLTVDGLE KERDFYFSKL RDIELICQEH
250 260 270 280
ESENSPVISG IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y