AiPD: Autoinhibited Protein Database

Q9UIK4

Gene name	DAPK2
Protein name	Death-associated protein kinase 2
Names	DAP kinase 2, DAP-kinase-related protein 1, DRP-1
Species	Homo sapiens (Human)
KEGG Pathway	hsa:23604
EC number	2.7.11.1: Protein-serine/threonine kinases
Protein Class	SERINE/THREONINE-PROTEIN KINASE 17 (PTHR24342)

Descriptions

DAPK2 (also named DRP-1), Death-associated protein kinase 2, is a Ca2+/calmodulin (CaM)-regulated Ser/Thr kinase that functions as a positive mediator of programmed cell death. DAPK2 has a DAP kinase homology region, which contains the catalytic domain (protein kinase domain) and the CaM-regulatory region, and the remaining C-terminal part, which displays no homology to any known protein and is required for self-dimerization. When binding to CaM, DAPK2 phosphorylates an exogenous substrate, the myosin light chain, in a Ca2+/CaM-dependent manner. The truncated protein, deletion of the CaM-regulatory domain, was converted into a constitutively active kinase. The death-promoting effects of DAPK2 depend on the functionality of the catalytic domain and on the presence of the C-terminal tail, yet further deletion of the CaM-regulatory domain abrogated the requirement for the C-terminal tail. Furthermore, the autophosphorylation of the Ser318 (Q9UIK4-1) within CaM-regulatory domain restrains the ability of DAPK2 to induce apoptosis and the homodimerization at basal state.

Autoinhibitory domains (AIDs)

Target domain	23-285 (Protein kinase domain)
Relief mechanism	Partner binding, PTM
Assay	Deletion assay, Mutagenesis experiment, Structural analysis

AID

288-320 (CaM-regulatory segment)

Target domain

23-285 (Protein kinase domain)

Relief mechanism

Partner binding (CaM binding), PTM (Dephosphorylation of Ser318 in Q9UIK4-1)

Assay

Deletion assay, Mutagenesis experiment, Structural analysis

Accessory elements

170-192 (Activation loop from InterPro)

Target domain	23-285 (Protein kinase domain)
Relief mechanism
Assay

References

Patel AK et al. (2011) "Structure of the dimeric autoinhibited conformation of DAPK2, a pro-apoptotic protein kinase", Journal of molecular biology, 409, 369-83

Shohat G et al. (2002) "The DAP-kinase family of proteins: study of a novel group of calcium-regulated death-promoting kinases", Biochimica et biophysica acta, 1600, 45-50

Shani G et al. (2001) "Autophosphorylation restrains the apoptotic activity of DRP-1 kinase by controlling dimerization and calmodulin binding", The EMBO journal, 20, 1099-113

Inbal B et al. (2000) "Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis", Molecular and cellular biology, 20, 1044-54

Simon B et al. (2016) "Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites", Structure (London, England : 1993), 24, 851-61

Horvath M et al. (2021) "14-3-3 proteins inactivate DAPK2 by promoting its dimerization and protecting key regulatory phosphosites", Communications biology, 4, 986

Autoinhibited structure

Activated structure

12 structures for Q9UIK4

Entry ID	Method	Resolution	Chain	Position	Source
1WMK	X-ray	360 A	A/B/C/D/E/F/G/H	11-330	PDB
1WRZ	X-ray	200 A	B	312-330	PDB
1Z9X	X-ray	393 A	A/B/C	11-330	PDB
1ZUZ	X-ray	191 A	B	312-330	PDB
1ZWS	X-ray	290 A	A/B/C/D/E/F/G/H	11-297	PDB
2A27	X-ray	300 A	A/B/C/D/E/F/G/H	11-330	PDB
2A2A	X-ray	147 A	A/B/C/D	11-330	PDB
2CKE	X-ray	280 A	A/B/C/D	11-330	PDB
6PAW	X-ray	295 A	A/B/E/F	12-330	PDB
7A6R	X-ray	270 A	E/F/G/L	364-370	PDB
7A6Y	X-ray	250 A	J/K/L/M	364-370	PDB
AF-Q9UIK4-F1	Predicted				AlphaFoldDB

335 variants for Q9UIK4

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA392811558 rs1221746789	4	A>S		No	ClinGen gnomAD
CA7607334 rs745683631	6	M>I		No	ClinGen ExAC gnomAD
rs1567292867 CA392811525	6	M>K		No	ClinGen Ensembl
rs138812766 CA7607335	6	M>V		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs868708473 CA272154091	7	R>K		No	ClinGen Ensembl
rs779065304 CA7607333	9	P>S		No	ClinGen ExAC gnomAD
rs753893356 CA7607331	10	N>D		No	ClinGen ExAC gnomAD
CA7607332 rs753893356	10	N>Y		No	ClinGen ExAC gnomAD
CA7607330 rs143249575	11	M>I		No	ClinGen ESP ExAC
CA7607329 rs373328408	13	P>A		No	ClinGen ESP ExAC gnomAD
CA7607328 rs753196708	18	K>E		No	ClinGen ExAC gnomAD
rs1350630497 CA392811248	19	V>L		No	ClinGen gnomAD
CA7607327 rs768163831	21	D>A		No	ClinGen ExAC gnomAD
CA392811151 rs764926615	24	D>E		No	ClinGen ExAC TOPMed gnomAD
rs1384502271 CA392811123	26	G>*		No	ClinGen TOPMed gnomAD
CA7607322 rs776201652	26	G>A		No	ClinGen ExAC gnomAD
rs1384502271 CA392811129	26	G>R		No	ClinGen TOPMed gnomAD
CA272153978 rs547052927	28	E>G		No	ClinGen Ensembl
rs1218839314 CA392811073	29	L>P		No	ClinGen TOPMed
rs1412716184 CA392811055	31	S>I		No	ClinGen gnomAD
rs777820396 CA7607295	33	Q>*		No	ClinGen ExAC TOPMed gnomAD
CA272154591 rs547035765	34	F>S		No	ClinGen TOPMed
rs1219849942 CA392818454	35	A>T		No	ClinGen gnomAD
CA7607292 rs138347092	36	I>M		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7607293 rs748183558	36	I>V		No	ClinGen ExAC gnomAD
CA7607290 rs770946548	37	V>M		No	ClinGen ExAC TOPMed gnomAD
CA392818368 rs1290297325	39	K>*		No	ClinGen gnomAD
rs145891572 CA7607288	41	R>Q		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7607289 rs779268346	41	R>W		No	ClinGen ExAC TOPMed gnomAD
CA392818324 rs1190337657	42	E>A		No	ClinGen TOPMed
CA392818327 rs1301021605	42	E>K		No	ClinGen TOPMed gnomAD
CA272154552 rs922726909	44	S>N		No	ClinGen TOPMed
rs201545659 CA7607287	45	T>M		No	ClinGen 1000Genomes ExAC gnomAD
rs755773376 CA7607285	46	G>R		No	ClinGen ExAC gnomAD
rs200254684 CA7607284	49	Y>F		No	ClinGen 1000Genomes ExAC gnomAD
rs759435234 CA7607282	52	K>E		No	ClinGen ExAC gnomAD
CA7607280 rs766375326	53	F>S		No	ClinGen ExAC TOPMed gnomAD
CA392818231 rs1489707225	55	K>T		No	ClinGen gnomAD
rs1233092511 CA392818222	56	K>R		No	ClinGen gnomAD
rs773075712 CA272154487	57	R>G		No	ClinGen ExAC TOPMed gnomAD
CA7607277 rs8037614	57	R>Q		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs773075712 CA7607278	57	R>W		No	ClinGen ExAC TOPMed gnomAD
CA392818211 rs1595852061	58	Q>R		No	ClinGen Ensembl
CA392818207 rs1344771489	59	S>G		No	ClinGen gnomAD
rs1595852038 CA392818202	59	S>R		No	ClinGen Ensembl
rs56047843 CA392818200	60	R>G		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs145090475 CA7607274	60	R>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
RCV000962994 rs145090475 CA7607273	60	R>Q		No	ClinGen ClinVar 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
VAR_040436 rs56047843 CA7607275	60	R>W		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA7607270 rs193279006	61	A>G		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA7607271 rs193279006	61	A>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA392818186 rs1390407384	62	S>N		No	ClinGen gnomAD
rs200808263 CA392818177	63	R>G		No	ClinGen ExAC TOPMed gnomAD
CA272154426 rs922516659	63	R>Q		No	ClinGen TOPMed gnomAD
CA7607268 rs200808263	63	R>W		No	ClinGen ExAC TOPMed gnomAD
rs752384008 CA7607267	64	R>C		No	ClinGen ExAC TOPMed gnomAD
rs752384008 CA272154418	64	R>G		No	ClinGen ExAC TOPMed gnomAD
rs767183532 CA7607266	64	R>H		No	ClinGen ExAC TOPMed gnomAD
CA7607264 rs140788025	65	G>S		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1186320632 CA392818144	66	V>M		No	ClinGen gnomAD
rs117564318 COSM1678526 CA7607261	68	R>L	haematopoietic_and_lymphoid_tissue [Cosmic]	No	ClinGen cosmic curated 1000Genomes ESP ExAC TOPMed gnomAD
rs117564318 CA392818116	68	R>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
COSM1678527 rs117564318 CA7607260 RCV000958691	68	R>Q	kidney [Cosmic]	No	ClinGen cosmic curated ClinVar 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA7607262 rs766172088	68	R>W		No	ClinGen ExAC TOPMed gnomAD
CA7607258 rs762104677	69	E>K		No	ClinGen ExAC TOPMed gnomAD
CA7607257 rs754998016	70	E>D		No	ClinGen ExAC TOPMed gnomAD
rs754998016 CA392818085	70	E>D		No	ClinGen ExAC TOPMed gnomAD
rs769030125 CA7607256	71	I>T		No	ClinGen ExAC gnomAD
rs1262740918 CA392818082	71	I>V		No	ClinGen TOPMed gnomAD
CA7607254 rs572424184	72	E>K		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA7607250 rs373888721	73	R>L		No	ClinGen ExAC TOPMed gnomAD
CA7607251 rs373888721	73	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA7607252 rs553905161	73	R>W		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA392818039 rs1399354123	74	E>D		No	ClinGen TOPMed gnomAD
rs1025758946 CA272154287	75	V>A		No	ClinGen Ensembl
rs751495460 CA272154303	75	V>M		No	ClinGen Ensembl
CA392818020 rs1173834868	76	S>N		No	ClinGen gnomAD
rs1467988620 CA392818013	77	I>L		No	ClinGen gnomAD
rs371887575 CA7607248	77	I>T		No	ClinGen ESP ExAC TOPMed gnomAD
CA392818011 rs1467988620	77	I>V		No	ClinGen gnomAD
rs754642102 CA7607247	78	L>P		No	ClinGen ExAC gnomAD
rs1214974133 CA392817986	79	R>Q		No	ClinGen TOPMed
rs542492420 CA7607246	79	R>W		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA7607244 rs758239287	81	V>G		No	ClinGen ExAC gnomAD
CA392817964 rs1189525115	81	V>L		No	ClinGen TOPMed gnomAD
rs1189525115 CA392817967	81	V>M		No	ClinGen TOPMed gnomAD
rs565512081 CA7607243	82	L>V		No	ClinGen ExAC
rs753691119 CA7607241	84	H>P		No	ClinGen ExAC TOPMed gnomAD
rs753691119 CA7607240	84	H>R		No	ClinGen ExAC TOPMed gnomAD
CA392817893 rs1483513308	84	H>Y		No	ClinGen gnomAD
CA7607239 rs764352298	85	N>S		No	ClinGen ExAC TOPMed gnomAD
rs761014070 CA7607238	86	V>I		No	ClinGen ExAC gnomAD
CA392817798 rs1244714096	88	T>M		No	ClinGen TOPMed gnomAD
rs1335577218 CA392817764	90	H>P		No	ClinGen TOPMed gnomAD
rs1403509203 CA392817748	91	D>N		No	ClinGen TOPMed gnomAD
rs1428214628 CA392817704	92	V>I		No	ClinGen gnomAD
CA7607232 rs749599588	93	Y>*		No	ClinGen ExAC gnomAD
rs771436759 CA7607233	93	Y>C		No	ClinGen ExAC TOPMed gnomAD
CA7607231 rs778172105	94	E>D		No	ClinGen ExAC gnomAD
CA392817673 rs1457839415	94	E>K		No	ClinGen TOPMed
CA7607230 rs142526378	95	N>D		No	ClinGen ESP ExAC TOPMed gnomAD
rs201432861 CA7607229 COSM963901	96	R>H	endometrium [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA7607228 rs779409838	97	T>I		No	ClinGen ExAC gnomAD
rs62639967 CA7607225	98	D>E		No	ClinGen ExAC TOPMed gnomAD
rs62639967 CA392817634	98	D>E		No	ClinGen ExAC TOPMed gnomAD
rs148399921 CA7607227	98	D>N		No	ClinGen ExAC TOPMed gnomAD
rs749931095 CA7607226	98	D>V		No	ClinGen ExAC gnomAD
CA392817628 rs1567251119	99	V>G		No	ClinGen Ensembl
rs1275026560 CA392817633	99	V>M		No	ClinGen gnomAD
rs113989390 CA272154161	100	V>A		No	ClinGen Ensembl
CA392817627 rs1221512803	100	V>M		No	ClinGen gnomAD
CA392817609 rs1360419127	102	I>V		No	ClinGen TOPMed gnomAD
rs1285648001 CA392817579	104	E>K		No	ClinGen gnomAD
rs1567241612 CA392815243	107	S>C		No	ClinGen Ensembl
rs200251366 CA272144063	107	S>T		No	ClinGen gnomAD
rs267604283 CA272144060	108	G>R		No	ClinGen Ensembl
CA392815232 rs1464127465	109	G>E		No	ClinGen gnomAD
CA7607189 rs776872086	110	E>D		No	ClinGen ExAC gnomAD
rs190782744 CA7607190	110	E>K		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA7607188 rs144523094	112	F>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1219098505 CA392815174	113	D>E		No	ClinGen gnomAD
CA7607186 COSM1374014 rs553988389	113	D>N	large_intestine [Cosmic]	No	ClinGen cosmic curated 1000Genomes ExAC TOPMed gnomAD
CA272143984 rs982230487	115	L>P		No	ClinGen Ensembl
CA392815140 rs1276934278	116	A>D		No	ClinGen TOPMed gnomAD
CA392815138 rs1276934278	116	A>V		No	ClinGen TOPMed gnomAD
CA7607184 rs748827584	117	Q>R		No	ClinGen ExAC gnomAD
CA7607183 rs777810869	119	E>K		No	ClinGen ExAC
CA392815090 rs1595827035	120	S>A		No	ClinGen Ensembl
rs866675633 CA272143951	126	A>V		No	ClinGen TOPMed gnomAD
rs1271271008 CA392814901	131	K>R		No	ClinGen gnomAD
rs147197384 CA7607179	135	D>E		No	ClinGen ESP ExAC TOPMed gnomAD
rs534412704 CA7607178	136	G>W		No	ClinGen 1000Genomes ExAC gnomAD
rs573404934 CA272143909	137	V>L		No	ClinGen 1000Genomes gnomAD
CA272143890 rs573404934	137	V>L		No	ClinGen 1000Genomes gnomAD
CA392814758 rs1430081972	138	N>D		No	ClinGen TOPMed
CA392814708 rs1174029881	140	L>P		No	ClinGen TOPMed
CA392814673 rs555303465	142	T>A		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA7607175 rs555303465	142	T>P		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA272143871 rs1012797638	142	T>R		No	ClinGen TOPMed
rs958891636 CA272143857	143	K>E		No	ClinGen Ensembl
CA392814598 rs1451269491	144	K>E		No	ClinGen TOPMed gnomAD
rs1451269491 CA392814606	144	K>Q		No	ClinGen TOPMed gnomAD
rs750507019 CA7607174	148	F>L		No	ClinGen ExAC gnomAD
rs1049037874 CA272118583	152	P>L		No	ClinGen TOPMed
CA392808359 rs1409087544	154	N>D		No	ClinGen gnomAD
CA392808353 rs1348592797	154	N>K		No	ClinGen gnomAD
rs766120131 CA7607139	155	I>M		No	ClinGen ExAC gnomAD
CA392808347 rs967994152	155	I>S		No	ClinGen TOPMed
CA272118574 rs967994152	155	I>T		No	ClinGen TOPMed
CA7607137 rs201994664	159	D>H		No	ClinGen 1000Genomes ExAC gnomAD
rs372823645 CA272118564	160	K>N		No	ClinGen ESP
rs1257731531 CA392808302	161	N>K		No	ClinGen TOPMed
rs1414792210 CA392808305	161	N>S		No	ClinGen Ensembl
rs1484668545 CA392808294	163	P>A		No	ClinGen TOPMed
rs1162346140 CA392808287	164	I>V		No	ClinGen gnomAD
CA7607136 rs772751720	165	P>S		No	ClinGen ExAC gnomAD
rs563315259 CA392808270	166	H>Q		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1263653936 CA392808266	167	I>F		No	ClinGen TOPMed
CA7607134 rs551687423	170	I>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1472526459 CA392808233	172	F>L		No	ClinGen gnomAD
CA392808222 rs1250292910	173	G>A		No	ClinGen gnomAD
rs1184752169 CA392808219	174	L>V		No	ClinGen gnomAD
rs776392199 CA7607133	175	A>P		No	ClinGen ExAC TOPMed gnomAD
rs768587064 CA7607132	175	A>V		No	ClinGen ExAC gnomAD
CA7607130 rs186891659 COSM99056	176	H>Q	stomach [Cosmic]	No	ClinGen cosmic curated 1000Genomes ESP ExAC TOPMed gnomAD
CA7607128 rs777665315 COSM963900	177	E>K	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs539034378 CA272118479	178	I>M		No	ClinGen Ensembl
CA7607127 rs779027761	180	D>N		No	ClinGen ExAC TOPMed gnomAD
rs966172470 CA392808172	181	G>A		No	ClinGen gnomAD
CA272118463 rs966172470	181	G>E		No	ClinGen gnomAD
rs757231979 CA7607126	182	V>A		No	ClinGen ExAC gnomAD
rs778355750 CA7607124	186	N>D		No	ClinGen ExAC TOPMed gnomAD
CA272118444 rs1018727274	186	N>S		No	ClinGen Ensembl
rs1567219370 CA392808099	187	I>S		No	ClinGen Ensembl
rs756699976 CA7607123	187	I>V		No	ClinGen ExAC TOPMed gnomAD
CA392808056 rs149502968	190	T>K		No	ClinGen ESP ExAC TOPMed gnomAD
CA7607122 rs149502968 COSM1249683	190	T>M	oesophagus [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA7607121 rs768038957	191	P>L		No	ClinGen ExAC TOPMed gnomAD
CA392808044 rs768038957	191	P>Q		No	ClinGen ExAC TOPMed gnomAD
rs1157727883 CA392808049	191	P>S		No	ClinGen gnomAD
CA7607119 rs749996059	192	E>K		No	ClinGen ExAC gnomAD
CA7607117 rs761163560	194	V>A		No	ClinGen ExAC gnomAD
CA7607118 rs764973476	194	V>I		No	ClinGen ExAC TOPMed gnomAD
CA7607116 rs776220153	195	A>T		No	ClinGen ExAC gnomAD
CA7607094 rs775357202	199	V>M		No	ClinGen ExAC gnomAD
rs199809638 CA392817845	201	Y>*		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA7607090 rs771042818	202	E>D		No	ClinGen ExAC gnomAD
rs774205620 CA7607092	202	E>K		No	ClinGen ExAC TOPMed gnomAD
rs774205620 CA7607091	202	E>Q		No	ClinGen ExAC TOPMed gnomAD
rs1567211836 CA392817820	203	P>L		No	ClinGen Ensembl
CA272152338 rs960715632	203	P>S		No	ClinGen TOPMed gnomAD
CA392817790 rs1168378918	205	G>V		No	ClinGen TOPMed gnomAD
CA392817787 rs773406301	206	L>M		No	ClinGen ExAC TOPMed
rs773406301 CA7607088	206	L>V		No	ClinGen ExAC TOPMed
rs908869561 CA272152308	207	E>*		No	ClinGen Ensembl
rs1464279688 CA392817761	208	A>T		No	ClinGen TOPMed
CA7607039 rs751039423	211	W>*		No	ClinGen ExAC gnomAD
CA392817698 rs1423377033	211	W>L		No	ClinGen TOPMed gnomAD
rs1453148299 CA392817599	212	S>N		No	ClinGen TOPMed gnomAD
rs1454687800 CA392817594	212	S>R		No	ClinGen TOPMed
rs750240144 CA7607036	215	V>D		No	ClinGen ExAC TOPMed gnomAD
CA7607037 rs140850258	215	V>I		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA272151439 rs1016929635	216	I>L		No	ClinGen TOPMed
rs540274954 CA272151425	216	I>M		No	ClinGen Ensembl
CA7607035 rs750903042	217	T>A		No	ClinGen ExAC gnomAD
CA7607033 rs768962834	220	L>F		No	ClinGen ExAC gnomAD
CA392817362 rs1231573978	221	L>*		No	ClinGen TOPMed gnomAD
rs1231573978 CA392817365	221	L>*		No	ClinGen TOPMed gnomAD
CA392817360 rs1258389358	221	L>F		No	ClinGen gnomAD
rs1477637467 CA392817357	222	S>G		No	ClinGen TOPMed
rs1336825569 CA392817353	222	S>N		No	ClinGen gnomAD
CA7607012 rs756347717	223	G>E		No	ClinGen ExAC gnomAD
rs200851607 CA7607013	223	G>R		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA392817337 rs1348333783	224	A>T		No	ClinGen gnomAD
CA392817327 rs1299691764	225	S>T		No	ClinGen gnomAD
rs150637317 CA7607011	228	L>P		No	ClinGen ESP ExAC TOPMed gnomAD
CA392817272 rs1320991389	230	D>N		No	ClinGen gnomAD
COSM1740163 rs865951599 CA272148405	231	T>A	haematopoietic_and_lymphoid_tissue [Cosmic]	No	ClinGen cosmic curated Ensembl
rs767623056 CA7607010	231	T>M		No	ClinGen ExAC TOPMed gnomAD
rs766877161 CA7607007	235	T>A		No	ClinGen ExAC gnomAD
rs763320373 CA7607006	236	L>R		No	ClinGen ExAC gnomAD
CA392817196 rs1567207541	237	A>G		No	ClinGen Ensembl
rs1481063507 CA392817164	240	T>R		No	ClinGen gnomAD
CA392817159 rs773511101	241	A>S		No	ClinGen ExAC TOPMed gnomAD
rs773511101 CA7607005	241	A>T		No	ClinGen ExAC TOPMed gnomAD
CA7607003 rs746683156	242	V>A		No	ClinGen ExAC gnomAD
CA7607001 rs771656924	243	S>N		No	ClinGen ExAC TOPMed gnomAD
CA7606999 rs370739509	245	D>N		No	ClinGen ESP ExAC TOPMed gnomAD
CA7606997 rs749095099	248	E>K		No	ClinGen ExAC gnomAD
CA7606995 rs756326689	249	E>*		No	ClinGen ExAC gnomAD
rs1364959220 CA392817046	250	F>L		No	ClinGen TOPMed
rs1381817567 CA392817034	251	F>V		No	ClinGen TOPMed gnomAD
CA7606993 rs114893434	252	S>I		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7606992 rs754984894	253	Q>R		No	ClinGen ExAC gnomAD
rs766858663 CA7606990	254	T>M		No	ClinGen ExAC TOPMed gnomAD
rs751652141 CA7606991	254	T>P		No	ClinGen ExAC gnomAD
CA272148336 rs1009030721	255	S>G		No	ClinGen TOPMed
CA7606985 rs374682269	256	E>D		No	ClinGen ESP ExAC TOPMed gnomAD
CA7606986 rs374682269	256	E>D		No	ClinGen ESP ExAC TOPMed gnomAD
rs765572531 CA7606987	256	E>K		No	ClinGen ExAC TOPMed gnomAD
CA7606983 rs759054798	259	K>E		No	ClinGen ExAC TOPMed gnomAD
CA7606982 rs774062023	260	D>E		No	ClinGen ExAC gnomAD
CA392816910 rs1300994606	261	F>C		No	ClinGen gnomAD
rs1235974086 CA392816901	262	I>F		No	ClinGen gnomAD
rs749221840 CA7606980 COSM1374013	263	R>Q	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA392816890 rs1296183000	263	R>W		No	ClinGen gnomAD
rs777600109 CA7606979	266	L>P		No	ClinGen ExAC gnomAD
rs920576554 CA272148300	266	L>V		No	ClinGen TOPMed
CA272148290 rs376917770	268	K>E		No	ClinGen ESP
CA7606978 rs769438263	269	E>K		No	ClinGen ExAC gnomAD
rs1595717644 CA392816823	270	T>P		No	ClinGen Ensembl
rs755252272 CA7606975	271	R>Q		No	ClinGen ExAC gnomAD
rs34270163 VAR_040437 CA7606976	271	R>W		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA7606948 rs751205501	272	K>R		No	ClinGen ExAC TOPMed gnomAD
CA7606947 rs145658373	273	R>Q		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs957393859 CA272147255	273	R>W		No	ClinGen TOPMed gnomAD
rs1216934505 CA392816736	275	T>I		No	ClinGen TOPMed
CA7606945 rs139000467	276	I>T		No	ClinGen ESP ExAC TOPMed gnomAD
rs762314204 CA7606946	276	I>V		No	ClinGen ExAC gnomAD
CA392816727 rs1334749701	277	Q>P		No	ClinGen gnomAD
rs765081205 CA7606944	280	L>F		No	ClinGen ExAC gnomAD
CA392816697 rs1183708985	281	R>S		No	ClinGen Ensembl
CA392816696 rs761783860	282	H>N		No	ClinGen ExAC TOPMed gnomAD
rs1595714576 CA392816694	282	H>P		No	ClinGen Ensembl
CA7606943 rs761783860	282	H>Y		No	ClinGen ExAC TOPMed gnomAD
CA7606941 rs534854161	283	P>L		No	ClinGen ExAC TOPMed gnomAD
CA7606942 rs534854161	283	P>R		No	ClinGen ExAC TOPMed gnomAD
CA392816689 rs1258428061	283	P>T		No	ClinGen TOPMed gnomAD
rs1305966915 CA392816679	284	W>*		No	ClinGen gnomAD
rs775505588 CA7606939	286	T>M		No	ClinGen ExAC TOPMed gnomAD
rs368792810 CA7606873	287	P>L		No	ClinGen ESP ExAC TOPMed gnomAD
rs1458281547 CA392813067	288	V>A		No	ClinGen TOPMed gnomAD
CA7606870 rs747614906	288	V>M		No	ClinGen ExAC gnomAD
CA392813044 rs1596379988	290	N>T		No	ClinGen Ensembl
CA272138288 rs772407366	291	Q>E		No	ClinGen Ensembl
rs1278957682 CA392813008	293	A>S		No	ClinGen TOPMed
CA392813000 rs1567197503	294	M>V		No	ClinGen Ensembl
rs754938406 CA7606868	295	V>A		No	ClinGen ExAC TOPMed gnomAD
rs144968721 CA7606867	296	R>C		No	ClinGen ESP ExAC TOPMed gnomAD
rs766325954 CA7606866	296	R>H		No	ClinGen ExAC TOPMed gnomAD
rs766325954 CA272138285	296	R>L		No	ClinGen ExAC TOPMed gnomAD
CA7606865 rs370209874	298	E>K		No	ClinGen ESP ExAC TOPMed gnomAD
CA392812902 rs1273954258	302	N>H		No	ClinGen TOPMed
rs1468415003 CA392812885	303	L>P		No	ClinGen gnomAD
rs898019066 CA272138253	309	Q>P		No	ClinGen TOPMed
CA7606863 rs765387382	310	Y>F		No	ClinGen ExAC gnomAD
CA7606862 rs761897052	311	V>I		No	ClinGen ExAC gnomAD
rs776636585 CA7606861	312	R>C		No	ClinGen ExAC TOPMed gnomAD
CA7606860 rs764655277	312	R>H		No	ClinGen ExAC TOPMed gnomAD
rs1596379906 CA392812771	313	R>G		No	ClinGen Ensembl
rs150322169 CA392812757	314	R>P		No	ClinGen ESP ExAC TOPMed gnomAD
rs150322169 CA7606858	314	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
rs761122652 CA7606859	314	R>W		No	ClinGen ExAC gnomAD
rs772372955 CA7606857	315	W>*		No	ClinGen ExAC gnomAD
CA392812725 rs1168900199	316	K>R		No	ClinGen TOPMed
rs1340841459 CA392812682	317	L>F		No	ClinGen gnomAD
CA392812684 rs1340841459	317	L>I		No	ClinGen gnomAD
CA392812646 rs1412507270	320	S>N		No	ClinGen gnomAD
rs1042210268 CA272138080	321	I>F		No	ClinGen TOPMed
rs756118915 CA7606824	322	V>M		No	ClinGen ExAC TOPMed gnomAD
CA392812582 rs1169093593	326	N>S		No	ClinGen gnomAD
rs1479102715 CA392812570	327	H>R		No	ClinGen gnomAD
CA392812546 rs1192099476	329	T>I		No	ClinGen TOPMed
CA7606821 rs760077260	330	R>C		No	ClinGen ExAC TOPMed gnomAD
CA7606820 rs200630392	330	R>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs200773863 CA7606818	331	S>L		No	ClinGen ExAC TOPMed gnomAD
CA7606819 rs200773863	331	S>W		No	ClinGen ExAC TOPMed gnomAD
CA392812512 rs1256258715	333	M>T		No	ClinGen gnomAD
rs1200416756 CA392812500	334	K>R		No	ClinGen gnomAD
rs140149939 CA7606815	336	V>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs140149939 CA7606814	336	V>M		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7606812 rs558649459	337	H>Q		No	ClinGen ExAC TOPMed gnomAD
rs772100581 CA7606813	337	H>R		No	ClinGen ExAC TOPMed gnomAD
CA392812452 rs1284356028	339	R>K		No	ClinGen gnomAD
CA7606811 rs147774460	340	P>L		No	ClinGen ESP ExAC TOPMed gnomAD
rs1332837625 CA392812413	342	E>D		No	ClinGen gnomAD
rs749111392 CA7606809	342	E>K		No	ClinGen ExAC gnomAD
rs930363557 CA272137977	343	D>G		No	ClinGen gnomAD
CA392811905 rs1425868241	351	T>A		No	ClinGen gnomAD
CA7606782 rs781426710	352	E>*		No	ClinGen ExAC gnomAD
rs1482291474 CA392811868	354	D>N		No	ClinGen Ensembl
rs939539718 CA392811850	355	I>M		No	ClinGen TOPMed gnomAD
CA392811856 rs1316251049	355	I>V		No	ClinGen TOPMed gnomAD
CA7606781 rs140635820	356	A>T		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs530845034 CA7606780	357	R>G		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1485161842 CA392811798	360	A>D		No	ClinGen gnomAD
rs1567195534 CA392811791	361	L>F		No	ClinGen Ensembl
rs1211695047 CA392811781	362	H>Y		No	ClinGen gnomAD
CA392811766 rs1314460243	363	P>S		No	ClinGen TOPMed gnomAD
rs750726015 CA7606777	364	R>Q		No	ClinGen ExAC gnomAD
CA7606778 rs759014876	364	R>W		No	ClinGen ExAC gnomAD
CA392811751 rs1338843233	365	R>K		No	ClinGen gnomAD
CA7606776 rs765741864	366	R>K		No	ClinGen ExAC TOPMed gnomAD
CA7606774 rs752354265	368	S>G		No	ClinGen ExAC gnomAD
CA7606772 rs373230434	369	T>N		No	ClinGen ESP ExAC gnomAD

No associated diseases with Q9UIK4

3 regional properties for Q9UIK4

Type	Name	Position	InterPro Accession
domain	Protein kinase domain	23 - 285	IPR000719
active_site	Serine/threonine-protein kinase, active site	145 - 157	IPR008271
binding_site	Protein kinase, ATP binding site	29 - 56	IPR017441

Functions

		Description
EC Number	2.7.11.1	Protein-serine/threonine kinases
Subcellular Localization	Cytoplasm Cytoplasmic vesicle, autophagosome lumen
PANTHER Family	PTHR24342	SERINE/THREONINE-PROTEIN KINASE 17
PANTHER Subfamily	PTHR24342:SF15	DEATH-ASSOCIATED PROTEIN KINASE 2
PANTHER Protein Class	non-receptor serine/threonine protein kinase protein modifying enzyme
PANTHER Pathway Category	No pathway information available

6 GO annotations of cellular component

Name	Definition
autophagosome lumen	The volume enclosed within the autophagosome double-membrane.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic vesicle	A vesicle found in the cytoplasm of a cell.
Golgi apparatus	A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
intracellular membrane-bounded organelle	Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

6 GO annotations of molecular function

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calmodulin binding	Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
identical protein binding	Binding to an identical protein or proteins.
protein serine kinase activity	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity	Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

11 GO annotations of biological process

Name	Definition
anoikis	Apoptosis triggered by inadequate or inappropriate adherence to substrate e.g. after disruption of the interactions between normal epithelial cells and the extracellular matrix.
apoptotic process	A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
intracellular signal transduction	The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
positive regulation of apoptotic process	Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
positive regulation of eosinophil chemotaxis	Any process that activates or increases the frequency, rate or extent of eosinophil chemotaxis.
positive regulation of neutrophil chemotaxis	Any process that increases the frequency, rate, or extent of neutrophil chemotaxis. Neutrophil chemotaxis is the directed movement of a neutrophil cell, the most numerous polymorphonuclear leukocyte found in the blood, in response to an external stimulus, usually an infection or wounding.
protein autophosphorylation	The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
protein phosphorylation	The process of introducing a phosphate group on to a protein.
regulation of apoptotic process	Any process that modulates the occurrence or rate of cell death by apoptotic process.
regulation of autophagy	Any process that modulates the frequency, rate or extent of autophagy. Autophagy is the process in which cells digest parts of their own cytoplasm.
regulation of intrinsic apoptotic signaling pathway	Any process that modulates the frequency, rate or extent of intrinsic apoptotic signaling pathway.

99 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
A4IFM7	MYLK2	Myosin light chain kinase 2, skeletal/cardiac muscle	Bos taurus (Bovine)	SS
Q0V7M1	PSKH1	Serine/threonine-protein kinase H1	Bos taurus (Bovine)	SS
Q0VD22	STK33	Serine/threonine-protein kinase 33	Bos taurus (Bovine)	PR
Q96NX5	CAMK1G	Calcium/calmodulin-dependent protein kinase type 1G	Homo sapiens (Human)	SS
O15075	DCLK1	Serine/threonine-protein kinase DCLK1	Homo sapiens (Human)	EV
Q14012	CAMK1	Calcium/calmodulin-dependent protein kinase type 1	Homo sapiens (Human)	EV
Q86YV6	MYLK4	Myosin light chain kinase family member 4	Homo sapiens (Human)	SS
Q16566	CAMK4	Calcium/calmodulin-dependent protein kinase type IV	Homo sapiens (Human)	SS
P11801	PSKH1	Serine/threonine-protein kinase H1	Homo sapiens (Human)	SS
Q8N568	DCLK2	Serine/threonine-protein kinase DCLK2	Homo sapiens (Human)	PR
O75962	TRIO	Triple functional domain protein	Homo sapiens (Human)	EV
Q9H1R3	MYLK2	Myosin light chain kinase 2, skeletal/cardiac muscle	Homo sapiens (Human)	EV
Q32MK0	MYLK3	Myosin light chain kinase 3	Homo sapiens (Human)	SS
Q8WWF8	CAPSL	Calcyphosin-like protein	Homo sapiens (Human)	PR
Q6P2M8	PNCK	Calcium/calmodulin-dependent protein kinase type 1B	Homo sapiens (Human)	SS
Q9BYT3	STK33	Serine/threonine-protein kinase 33	Homo sapiens (Human)	PR
Q8NCB2	CAMKV	CaM kinase-like vesicle-associated protein	Homo sapiens (Human)	SS
Q8IU85	CAMK1D	Calcium/calmodulin-dependent protein kinase type 1D	Homo sapiens (Human)	SS
P53355	DAPK1	Death-associated protein kinase 1	Homo sapiens (Human)	EV
O43293	DAPK3	Death-associated protein kinase 3	Homo sapiens (Human)	PR
O94768	STK17B	Serine/threonine-protein kinase 17B	Homo sapiens (Human)	PR
Q9UEE5	STK17A	Serine/threonine-protein kinase 17A	Homo sapiens (Human)	SS
Q9QYK9	Pnck	Calcium/calmodulin-dependent protein kinase type 1B	Mus musculus (Mouse)	PR
Q91VB2	Camk1g	Calcium/calmodulin-dependent protein kinase type 1G	Mus musculus (Mouse)	SS
Q8BG48	Stk17b	Serine/threonine-protein kinase 17B	Mus musculus (Mouse)	PR
Q8VCR8	Mylk2	Myosin light chain kinase 2, skeletal/cardiac muscle	Mus musculus (Mouse)	SS
Q6PGN3	Dclk2	Serine/threonine-protein kinase DCLK2	Mus musculus (Mouse)	PR
Q91YS8	Camk1	Calcium/calmodulin-dependent protein kinase type 1	Mus musculus (Mouse)	SS
Q3UIZ8	Mylk3	Myosin light chain kinase 3	Mus musculus (Mouse)	SS
Q6P8Y1	Capsl	Calcyphosin-like protein	Mus musculus (Mouse)	PR
Q8BW96	Camk1d	Calcium/calmodulin-dependent protein kinase type 1D	Mus musculus (Mouse)	SS
Q3UHL1	Camkv	CaM kinase-like vesicle-associated protein	Mus musculus (Mouse)	SS
P08414	Camk4	Calcium/calmodulin-dependent protein kinase type IV	Mus musculus (Mouse)	SS
Q9JLM8	Dclk1	Serine/threonine-protein kinase DCLK1	Mus musculus (Mouse)	SS
Q91YA2	Pskh1	Serine/threonine-protein kinase H1	Mus musculus (Mouse)	SS
Q80YE7	Dapk1	Death-associated protein kinase 1	Mus musculus (Mouse)	SS
Q62407	Speg	Striated muscle-specific serine/threonine-protein kinase	Mus musculus (Mouse)	SS
Q0KL02	Trio	Triple functional domain protein	Mus musculus (Mouse)	SS
Q924X7	Stk33	Serine/threonine-protein kinase 33	Mus musculus (Mouse)	PR
O54784	Dapk3	Death-associated protein kinase 3	Mus musculus (Mouse)	PR
Q8VDF3	Dapk2	Death-associated protein kinase 2	Mus musculus (Mouse)	EV
Q7TNJ7	Camk1g	Calcium/calmodulin-dependent protein kinase type 1G	Rattus norvegicus (Rat)	SS
P20689	Mylk2	Myosin light chain kinase 2, skeletal/cardiac muscle	Rattus norvegicus (Rat)	SS
F1M0Z1	Trio	Triple functional domain protein	Rattus norvegicus (Rat)	SS
Q91XS8	Stk17b	Serine/threonine-protein kinase 17B	Rattus norvegicus (Rat)	PR
O08875	Dclk1	Serine/threonine-protein kinase DCLK1	Rattus norvegicus (Rat)	PR
E9PT87	Mylk3	Myosin light chain kinase 3	Rattus norvegicus (Rat)	SS
O70150	Pnck	Calcium/calmodulin-dependent protein kinase type 1B	Rattus norvegicus (Rat)	PR
Q63092	Camkv	CaM kinase-like vesicle-associated protein	Rattus norvegicus (Rat)	SS
Q63638	Speg	Striated muscle-specific serine/threonine-protein kinase	Rattus norvegicus (Rat)	SS
O88764	Dapk3	Death-associated protein kinase 3	Rattus norvegicus (Rat)	PR
P13234	Camk4	Calcium/calmodulin-dependent protein kinase type IV	Rattus norvegicus (Rat)	SS
Q63450	Camk1	Calcium/calmodulin-dependent protein kinase type 1	Rattus norvegicus (Rat)	EV
P97924	Kalrn	Kalirin	Rattus norvegicus (Rat)	SS
Q5MPA9	Dclk2	Serine/threonine-protein kinase DCLK2	Rattus norvegicus (Rat)	PR
Q5VQQ5	CPK2	Calcium-dependent protein kinase 2	Oryza sativa subsp. japonica (Rice)	SS
Q8LPZ7	CPK3	Calcium-dependent protein kinase 3	Oryza sativa subsp. japonica (Rice)	SS
Q6K968	CPK6	Calcium-dependent protein kinase 6	Oryza sativa subsp. japonica (Rice)	SS
P53684	CPK7	Calcium-dependent protein kinase 7	Oryza sativa subsp japonica (Rice)	PR
Q75GE8	CPK8	Calcium-dependent protein kinase 8	Oryza sativa subsp. japonica (Rice)	SS
Q6I5I8	CPK16	Calcium-dependent protein kinase 16	Oryza sativa subsp. japonica (Rice)	SS
Q6AVM3	CCAMK	Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase	Oryza sativa subsp japonica (Rice)	PR
Q84SL0	CPK20	Calcium-dependent protein kinase 20	Oryza sativa subsp. japonica (Rice)	SS
P53682	CPK23	Calcium-dependent protein kinase 23	Oryza sativa subsp japonica (Rice)	PR
Q9TXJ0	cmk-1	Calcium/calmodulin-dependent protein kinase type 1	Caenorhabditis elegans	PR
Q95QC4	zyg-8	Serine/threonine-protein kinase zyg-8	Caenorhabditis elegans	PR
O44997	dapk-1	Death-associated protein kinase dapk-1	Caenorhabditis elegans	SS
P28583		Calcium-dependent protein kinase SK5	Glycine max (Soybean) (Glycine hispida)	PR
Q9M9V8	CPK10	Calcium-dependent protein kinase 10	Arabidopsis thaliana (Mouse-ear cress)	PR
Q39016	CPK11	Calcium-dependent protein kinase 11	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9FX86	CRK8	CDPK-related kinase 8	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9SSF8	CPK30	Calcium-dependent protein kinase 30	Arabidopsis thaliana (Mouse-ear cress)	PR
Q38872	CPK6	Calcium-dependent protein kinase 6	Arabidopsis thaliana (Mouse-ear cress)	PR
Q7XJR9	CPK16	Calcium-dependent protein kinase 16	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9SIQ7	CPK24	Calcium-dependent protein kinase 24	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9SJ61	CPK25	Calcium-dependent protein kinase 25	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9ZV15	CPK20	Calcium-dependent protein kinase 20	Arabidopsis thaliana (Mouse-ear cress)	PR
P93759	GK-1	Calcium-dependent protein kinase 14	Arabidopsis thaliana (Mouse-ear cress)	PR
Q8S8S2	LPEAT2	Lysophospholipid acyltransferase LPEAT2	Arabidopsis thaliana (Mouse-ear cress)	PR
Q38870	CPK2	Calcium-dependent protein kinase 2	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9LJL9	CRK2	CDPK-related kinase 2	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9SG12	CRK6	CDPK-related kinase 6	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9SCS2	CRK5	CDPK-related kinase 5	Arabidopsis thaliana (Mouse-ear cress)	PR
Q8W4I7	CPK13	Calcium-dependent protein kinase 13	Arabidopsis thaliana (Mouse-ear cress)	PR
Q6NLQ6	CPK32	Calcium-dependent protein kinase 32	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9ZSA4	CPK27	Calcium-dependent protein kinase 27	Arabidopsis thaliana (Mouse-ear cress)	PR
Q38869	CPK4	Calcium-dependent protein kinase 4	Arabidopsis thaliana (Mouse-ear cress)	SS
Q42479	CPK3	Calcium-dependent protein kinase 3	Arabidopsis thaliana (Mouse-ear cress)	PR
Q1PE17	CPK18	Calcium-dependent protein kinase 18	Arabidopsis thaliana (Mouse-ear cress)	SS
Q06850	CPK1	Calcium-dependent protein kinase 1	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9FMP5	CPK17	Calcium-dependent protein kinase 17	Arabidopsis thaliana (Mouse-ear cress)	PR
Q38873	CPK7	Calcium-dependent protein kinase 7	Arabidopsis thaliana (Mouse-ear cress)	PR
Q3E9C0	CPK34	Calcium-dependent protein kinase 34	Arabidopsis thaliana (Mouse-ear cress)	PR
Q42438	CPK8	Calcium-dependent protein kinase 8	Arabidopsis thaliana (Mouse-ear cress)	PR
Q42396	CPK12	Calcium-dependent protein kinase 12	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9FKW4	CPK28	Calcium-dependent protein kinase 28	Arabidopsis thaliana (Mouse-ear cress)	SS
Q1LUA6	trio	Triple functional domain protein	Danio rerio (Zebrafish) (Brachydanio rerio)	SS
A8C984	mylk3	Myosin light chain kinase 3	Danio rerio (Zebrafish) (Brachydanio rerio)	SS
Q7SY49	camkv	CaM kinase-like vesicle-associated protein	Danio rerio (Zebrafish) (Brachydanio rerio)	SS

10	20	30	40	50	60
MFQASMRSPN	MEPFKQQKVE	DFYDIGEELG	SGQFAIVKKC	REKSTGLEYA	AKFIKKRQSR
70	80	90	100	110	120
ASRRGVSREE	IEREVSILRQ	VLHHNVITLH	DVYENRTDVV	LILELVSGGE	LFDFLAQKES
130	140	150	160	170	180
LSEEEATSFI	KQILDGVNYL	HTKKIAHFDL	KPENIMLLDK	NIPIPHIKLI	DFGLAHEIED
190	200	210	220	230	240
GVEFKNIFGT	PEFVAPEIVN	YEPLGLEADM	WSIGVITYIL	LSGASPFLGD	TKQETLANIT
250	260	270	280	290	300
AVSYDFDEEF	FSQTSELAKD	FIRKLLVKET	RKRLTIQEAL	RHPWITPVDN	QQAMVRRESV
310	320	330	340	350	360
VNLENFRKQY	VRRRWKLSFS	IVSLCNHLTR	SLMKKVHLRP	DEDLRNCESD	TEEDIARRKA

LHPRRRSSTS