Q9U8Q4

EV
Gene name
Protein name
Pierisin
Names
EC 2.4.2.- , NAD--DNA ADP-ribosyltransferase , Pierisin-1
Species
Pieris rapae (Small white butterfly) (Artogeia rapae)
KEGG Pathway
EC number
2.4.2.-: Pentosyltransferases
Protein Class

Descriptions

Pierisin-1 is an ADP-ribosyltransferase that induces apoptosis of various types of cancer cell lines such as HeLa and TMK-1 cells. Segments 245-256 of the linker region mask the putative DNA-binding region and inhibit DNA binding, affecting ADP-ribosylation and apoptosis. This segment can also interact with the PN loop and induce a β4’ strand to form a β’-sheet, stabilizing the PN loop and relieving the autoinhibition of Pierisin-1.

Autoinhibitory domains (AIDs)

245-256 (Segment of linker region) EV

Target domain

1-233 (Catalytic domain)

Relief mechanism

Others

Assay

Structural analysis, Deletion assay, Mutagenesis experiment

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

6 structures for Q9U8Q4

Entry ID Method Resolution Chain Position Source
5H6J X-ray 190 A A/B 1-233 PDB
5H6K X-ray 180 A A/B 1-233 PDB
5H6L X-ray 210 A A/B 1-233 PDB
5H6M X-ray 190 A A/B 1-233 PDB
5H6N X-ray 180 A A/B/C/D 1-267 PDB
AF-Q9U8Q4-F1 Predicted AlphaFoldDB

No variants for Q9U8Q4

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9U8Q4

No associated diseases with Q9U8Q4

4 regional properties for Q9U8Q4

Type Name Position InterPro Accession
domain Ricin B, lectin domain 267 - 408 IPR000772-1
domain Ricin B, lectin domain 418 - 560 IPR000772-2
domain Ricin B, lectin domain 569 - 707 IPR000772-3
domain Ricin B, lectin domain 716 - 848 IPR000772-4

Functions

Description
EC Number 2.4.2.- Pentosyltransferases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

No GO annotations of cellular component

Name Definition
No GO annotations for cellular component

3 GO annotations of molecular function

Name Definition
2'-deoxyguanosine DNA ADP-ribosyltransferase activity Catalysis of the transfer of the ADP-ribose group of NAD+ to the amino group at N2 of 2'-deoxyguanosine to yield N2-(alpha-ADP-ribos-1-yl)-2'-deoxyguanosine and its beta form.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
nucleotidyltransferase activity Catalysis of the transfer of a nucleotidyl group to a reactant.

2 GO annotations of biological process

Name Definition
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
DNA ADP-ribosylation The covalent attachment of an ADP-ribosyl group to a residue in double-stranded DNA.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MADRQPYMTN GIQAAVVEWI RALDLEIISL LLSRAWPMAL LATSELRWRP TVLTDTDNVV
70 80 90 100 110 120
RLDRRQRLVR WDRRPPNEIF LDGFVPIVTR ENPDWEETDL YGFAKNNHPS IFVSTTKTQR
130 140 150 160 170 180
NKKKYVWTPR NANRGIVYQY EIYAPGGVDV NDSFSDASPW PNQMEVAFPG GIQNIYIRSA
190 200 210 220 230 240
RELHNGRIQR IWINPNFLDP GDLEPIVSSS RTPQVIWRMN HPDGGHRDQR SERSASSYDD
250 260 270 280 290 300
LMYGGTGNVQ EDTFGDEPNN PKPIAAGEFM IESIKDKNSF LDLSKNVNGG VIHSNLYSGG
310 320 330 340 350 360
DNQIWVFSYD DNKKAYRIQS YQNSYLYLSW DSNASSKEMI LRGYTNSGSN NQYWQIEQTG
370 380 390 400 410 420
KNYRLRNLLN LDMIITAQDK PSAFGGKEVI VNTEISNSNT KISQEWKMIP FDFRPIIDGD
430 440 450 460 470 480
YNIFNVDLSN QVVDFSNQPD LLVHGHIFCD NENQTWHFTY NSTYHAYKIW SGRKSNLLLT
490 500 510 520 530 540
WDSNAASKEM VVRAYTESRS KNQYWRIEQT GSKSYKVRNL ENSSMILGLT RVSTPYGGLN
550 560 570 580 590 600
LMVEDDSDGH SDLHSDWDIK PIFYQDIPDG DYNIFNDNFP NIAIDFTNQE GSLIHGHNFC
610 620 630 640 650 660
SNNNQKWSFV FDGKRKAYRI KSGVRSNLWL SWDSNASSKE MVLRAYTESG SSNQYWRLDE
670 680 690 700 710 720
ANDGSYRIRN LQDYYKLIAL TNKNTPYGGK ELIVSDNKES GNTWYLKKLG EVPLPNRKFR
730 740 750 760 770 780
IATKLNYKKV IDSSTSYNLI ITHDLNFASS IWELVYDSSK KAYNIYSSDI NNLGWIYQNK
790 800 810 820 830 840
NFFVKLGNID GPDHGDLRYF WTIEYSMQTG CYLIRSLHDP ANAVGYTDSE SVITDTSTYS
DNQLFHFILM