Q9TXJ0

PR
Gene name
cmk-1
Protein name
Calcium/calmodulin-dependent protein kinase type 1
Names
CaM kinase I, CaM-KI
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_K07A9.2
EC number
2.7.11.17: Protein-serine/threonine kinases
Protein Class

Descriptions

(Annotation based on sequence homology with Q9H1R3)
Myosin light chain kinases (MLCK) are members of the family of Ca2+-calmodulin-dependent protein kinases. This enzyme contains an autoinhibitory pseudosubstrate sequence that blocks the substrate-binding site of the kinase. The autoinhibitory sequence makes an extensive network of contacts on the surface of the larger C-domain of the catalytic core extending towards the catalytic cleft with the CaM-binding sequence to block the regulatory light chain of sarcomeric myosin, but not ATP binding in the catalytic core N-domain.

Autoinhibitory domains (AIDs)

276-348 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

164-185 (Activation loop from InterPro)

Target domain

22-278 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q9TXJ0

Entry ID Method Resolution Chain Position Source
AF-Q9TXJ0-F1 Predicted AlphaFoldDB

No variants for Q9TXJ0

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9TXJ0

No associated diseases with Q9TXJ0

3 regional properties for Q9TXJ0

Type Name Position InterPro Accession
domain Protein kinase domain 22 - 278 IPR000719
active_site Serine/threonine-protein kinase, active site 140 - 152 IPR008271
binding_site Protein kinase, ATP binding site 28 - 52 IPR017441

Functions

Description
EC Number 2.7.11.17 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
  • Cytoplasm
  • Localization is regulated by temperature in thermosensory neurons (PubMed:25467978, PubMed:25467982)
  • Localizes to the nucleus upon elevated temperature shift (PubMed:25467978)
  • Translocates from cytoplasm to the nucleus of FLP thermal nociceptors during thermal adaptation (PubMed:25467982)
  • Translocates transiently from cytoplasm to the nucleus of AWC sensory neurons in response to food withdrawal
  • Prolonged starvation results in cytoplasmic enrichment (PubMed:26335407)
  • The distribution between cytoplasm and nucleus is regulated by synaptic glutamate receptor glr-1 levels in PVC neurons, accumulating in the nucleus with increasing glr-1 (PubMed:27462879)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
calmodulin-dependent protein kinase activity Calmodulin-dependent catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; and ATP + a protein threonine = ADP + protein threonine phosphate.
metal ion binding Binding to a metal ion.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.

7 GO annotations of biological process

Name Definition
neuron projection morphogenesis The process in which the anatomical structures of a neuron projection are generated and organized. A neuron projection is any process extending from a neural cell, such as axons or dendrites.
positive regulation of CREB transcription factor activity Any process that activates or increases the frequency, rate or extent of activity of the transcription factor CREB.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of neuron differentiation Any process that modulates the frequency, rate or extent of neuron differentiation.
thermosensory behavior Behavior that is dependent upon the sensation of temperature.

100 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
A4IFM7 MYLK2 Myosin light chain kinase 2, skeletal/cardiac muscle Bos taurus (Bovine) SS
Q0V7M1 PSKH1 Serine/threonine-protein kinase H1 Bos taurus (Bovine) SS
Q0VD22 STK33 Serine/threonine-protein kinase 33 Bos taurus (Bovine) PR
O15075 DCLK1 Serine/threonine-protein kinase DCLK1 Homo sapiens (Human) EV
Q9UIK4 DAPK2 Death-associated protein kinase 2 Homo sapiens (Human) EV
O94768 STK17B Serine/threonine-protein kinase 17B Homo sapiens (Human) PR
Q86YV6 MYLK4 Myosin light chain kinase family member 4 Homo sapiens (Human) SS
Q16566 CAMK4 Calcium/calmodulin-dependent protein kinase type IV Homo sapiens (Human) SS
P11801 PSKH1 Serine/threonine-protein kinase H1 Homo sapiens (Human) SS
Q8N568 DCLK2 Serine/threonine-protein kinase DCLK2 Homo sapiens (Human) PR
Q9UEE5 STK17A Serine/threonine-protein kinase 17A Homo sapiens (Human) SS
O75962 TRIO Triple functional domain protein Homo sapiens (Human) EV
Q9H1R3 MYLK2 Myosin light chain kinase 2, skeletal/cardiac muscle Homo sapiens (Human) EV
Q32MK0 MYLK3 Myosin light chain kinase 3 Homo sapiens (Human) SS
Q8WWF8 CAPSL Calcyphosin-like protein Homo sapiens (Human) PR
P53355 DAPK1 Death-associated protein kinase 1 Homo sapiens (Human) EV
Q9BYT3 STK33 Serine/threonine-protein kinase 33 Homo sapiens (Human) PR
O43293 DAPK3 Death-associated protein kinase 3 Homo sapiens (Human) PR
Q6P2M8 PNCK Calcium/calmodulin-dependent protein kinase type 1B Homo sapiens (Human) SS
Q14012 CAMK1 Calcium/calmodulin-dependent protein kinase type 1 Homo sapiens (Human) EV
Q96NX5 CAMK1G Calcium/calmodulin-dependent protein kinase type 1G Homo sapiens (Human) SS
Q8IU85 CAMK1D Calcium/calmodulin-dependent protein kinase type 1D Homo sapiens (Human) SS
Q8NCB2 CAMKV CaM kinase-like vesicle-associated protein Homo sapiens (Human) SS
Q8BG48 Stk17b Serine/threonine-protein kinase 17B Mus musculus (Mouse) PR
Q8VCR8 Mylk2 Myosin light chain kinase 2, skeletal/cardiac muscle Mus musculus (Mouse) SS
Q6PGN3 Dclk2 Serine/threonine-protein kinase DCLK2 Mus musculus (Mouse) PR
Q3UIZ8 Mylk3 Myosin light chain kinase 3 Mus musculus (Mouse) SS
Q8VDF3 Dapk2 Death-associated protein kinase 2 Mus musculus (Mouse) EV
Q6P8Y1 Capsl Calcyphosin-like protein Mus musculus (Mouse) PR
P08414 Camk4 Calcium/calmodulin-dependent protein kinase type IV Mus musculus (Mouse) SS
Q9JLM8 Dclk1 Serine/threonine-protein kinase DCLK1 Mus musculus (Mouse) SS
Q91YA2 Pskh1 Serine/threonine-protein kinase H1 Mus musculus (Mouse) SS
Q80YE7 Dapk1 Death-associated protein kinase 1 Mus musculus (Mouse) SS
Q62407 Speg Striated muscle-specific serine/threonine-protein kinase Mus musculus (Mouse) SS
Q0KL02 Trio Triple functional domain protein Mus musculus (Mouse) SS
Q924X7 Stk33 Serine/threonine-protein kinase 33 Mus musculus (Mouse) PR
O54784 Dapk3 Death-associated protein kinase 3 Mus musculus (Mouse) PR
Q3UHL1 Camkv CaM kinase-like vesicle-associated protein Mus musculus (Mouse) SS
Q91VB2 Camk1g Calcium/calmodulin-dependent protein kinase type 1G Mus musculus (Mouse) SS
Q91YS8 Camk1 Calcium/calmodulin-dependent protein kinase type 1 Mus musculus (Mouse) SS
Q9QYK9 Pnck Calcium/calmodulin-dependent protein kinase type 1B Mus musculus (Mouse) PR
Q8BW96 Camk1d Calcium/calmodulin-dependent protein kinase type 1D Mus musculus (Mouse) SS
P20689 Mylk2 Myosin light chain kinase 2, skeletal/cardiac muscle Rattus norvegicus (Rat) SS
F1M0Z1 Trio Triple functional domain protein Rattus norvegicus (Rat) SS
Q91XS8 Stk17b Serine/threonine-protein kinase 17B Rattus norvegicus (Rat) PR
O08875 Dclk1 Serine/threonine-protein kinase DCLK1 Rattus norvegicus (Rat) PR
E9PT87 Mylk3 Myosin light chain kinase 3 Rattus norvegicus (Rat) SS
Q63638 Speg Striated muscle-specific serine/threonine-protein kinase Rattus norvegicus (Rat) SS
O88764 Dapk3 Death-associated protein kinase 3 Rattus norvegicus (Rat) PR
P13234 Camk4 Calcium/calmodulin-dependent protein kinase type IV Rattus norvegicus (Rat) SS
P97924 Kalrn Kalirin Rattus norvegicus (Rat) SS
Q5MPA9 Dclk2 Serine/threonine-protein kinase DCLK2 Rattus norvegicus (Rat) PR
O70150 Pnck Calcium/calmodulin-dependent protein kinase type 1B Rattus norvegicus (Rat) PR
Q63450 Camk1 Calcium/calmodulin-dependent protein kinase type 1 Rattus norvegicus (Rat) EV
Q7TNJ7 Camk1g Calcium/calmodulin-dependent protein kinase type 1G Rattus norvegicus (Rat) SS
Q63092 Camkv CaM kinase-like vesicle-associated protein Rattus norvegicus (Rat) SS
Q6AVM3 CCAMK Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase Oryza sativa subsp japonica (Rice) PR
P53682 CPK23 Calcium-dependent protein kinase 23 Oryza sativa subsp japonica (Rice) PR
P53684 CPK7 Calcium-dependent protein kinase 7 Oryza sativa subsp japonica (Rice) PR
Q5VQQ5 CPK2 Calcium-dependent protein kinase 2 Oryza sativa subsp. japonica (Rice) SS
Q6I5I8 CPK16 Calcium-dependent protein kinase 16 Oryza sativa subsp. japonica (Rice) SS
Q6K968 CPK6 Calcium-dependent protein kinase 6 Oryza sativa subsp. japonica (Rice) SS
Q75GE8 CPK8 Calcium-dependent protein kinase 8 Oryza sativa subsp. japonica (Rice) SS
Q84SL0 CPK20 Calcium-dependent protein kinase 20 Oryza sativa subsp. japonica (Rice) SS
Q8LPZ7 CPK3 Calcium-dependent protein kinase 3 Oryza sativa subsp. japonica (Rice) SS
O44997 dapk-1 Death-associated protein kinase dapk-1 Caenorhabditis elegans SS
Q95QC4 zyg-8 Serine/threonine-protein kinase zyg-8 Caenorhabditis elegans PR
O62305 unc-43 Calcium/calmodulin-dependent protein kinase type II Caenorhabditis elegans EV
P28583 Calcium-dependent protein kinase SK5 Glycine max (Soybean) (Glycine hispida) PR
Q9FX86 CRK8 CDPK-related kinase 8 Arabidopsis thaliana (Mouse-ear cress) PR
Q8S8S2 LPEAT2 Lysophospholipid acyltransferase LPEAT2 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LJL9 CRK2 CDPK-related kinase 2 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SG12 CRK6 CDPK-related kinase 6 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SCS2 CRK5 CDPK-related kinase 5 Arabidopsis thaliana (Mouse-ear cress) PR
Q42438 CPK8 Calcium-dependent protein kinase 8 Arabidopsis thaliana (Mouse-ear cress) PR
Q06850 CPK1 Calcium-dependent protein kinase 1 Arabidopsis thaliana (Mouse-ear cress) PR
Q38869 CPK4 Calcium-dependent protein kinase 4 Arabidopsis thaliana (Mouse-ear cress) SS
Q38870 CPK2 Calcium-dependent protein kinase 2 Arabidopsis thaliana (Mouse-ear cress) PR
Q38872 CPK6 Calcium-dependent protein kinase 6 Arabidopsis thaliana (Mouse-ear cress) PR
Q39016 CPK11 Calcium-dependent protein kinase 11 Arabidopsis thaliana (Mouse-ear cress) SS
Q3E9C0 CPK34 Calcium-dependent protein kinase 34 Arabidopsis thaliana (Mouse-ear cress) PR
Q42396 CPK12 Calcium-dependent protein kinase 12 Arabidopsis thaliana (Mouse-ear cress) SS
Q42479 CPK3 Calcium-dependent protein kinase 3 Arabidopsis thaliana (Mouse-ear cress) PR
Q7XJR9 CPK16 Calcium-dependent protein kinase 16 Arabidopsis thaliana (Mouse-ear cress) PR
Q8W4I7 CPK13 Calcium-dependent protein kinase 13 Arabidopsis thaliana (Mouse-ear cress) PR
Q9FMP5 CPK17 Calcium-dependent protein kinase 17 Arabidopsis thaliana (Mouse-ear cress) PR
Q9M9V8 CPK10 Calcium-dependent protein kinase 10 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SIQ7 CPK24 Calcium-dependent protein kinase 24 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SJ61 CPK25 Calcium-dependent protein kinase 25 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SSF8 CPK30 Calcium-dependent protein kinase 30 Arabidopsis thaliana (Mouse-ear cress) PR
Q9ZSA4 CPK27 Calcium-dependent protein kinase 27 Arabidopsis thaliana (Mouse-ear cress) PR
Q9ZV15 CPK20 Calcium-dependent protein kinase 20 Arabidopsis thaliana (Mouse-ear cress) PR
P93759 GK-1 Calcium-dependent protein kinase 14 Arabidopsis thaliana (Mouse-ear cress) PR
Q1PE17 CPK18 Calcium-dependent protein kinase 18 Arabidopsis thaliana (Mouse-ear cress) SS
Q38873 CPK7 Calcium-dependent protein kinase 7 Arabidopsis thaliana (Mouse-ear cress) PR
Q6NLQ6 CPK32 Calcium-dependent protein kinase 32 Arabidopsis thaliana (Mouse-ear cress) PR
Q9FKW4 CPK28 Calcium-dependent protein kinase 28 Arabidopsis thaliana (Mouse-ear cress) SS
Q1LUA6 trio Triple functional domain protein Danio rerio (Zebrafish) (Brachydanio rerio) SS
A8C984 mylk3 Myosin light chain kinase 3 Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q7SY49 camkv CaM kinase-like vesicle-associated protein Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MPLFKRRDGS GPAPNATIRE KYDFRDVLGT GAFSKVFLAE SKSDAGQMYA VKCIDKKALK
70 80 90 100 110 120
GKEESLENEI KVLRKLRHNN IVQLFDTYDE KQFVYLVMEL VTGGELFDRI VAKGSYTEQD
130 140 150 160 170 180
ASNLIRQVLE AVGFMHDNGV VHRDLKPENL LYYNQDEDSK IMISDFGLSK TEDSGVMATA
190 200 210 220 230 240
CGTPGYVAPE VLQQKPYGKA VDVWSIGVIA YILLCGYPPF YDESDANLFA QIIKGEYEFD
250 260 270 280 290 300
APYWDQISDS AKDFITHLMC CDPEARFTCQ DALSHPWISG NTAYTHDIHG TVAVHLKKSL
310 320 330 340
AKRNWKKAYN AAAAIRQLQM LRLSSNSNRL QKQASQQQPE PPTPAFHA