Q9SKI2

SS
Gene name
VPS2.1 (CHMP2-1, SLP, At2g06530, T12H3.8)
Protein name
Vacuolar protein sorting-associated protein 2 homolog 1
Names
AtVPS2-1 , Charged multivesicular body protein 2 homolog 1 , ESCRT-III complex subunit VPS2 homolog 1 , SNF7-like protein
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT2G06530
EC number
Protein Class

Descriptions

CHMP2A is a core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. Removing 42 amino acids from the C-terminal of the protein unmasks a common ability to associate with endosomal membranes and assemble into large polymeric complexes, and the C-terminal sequences are also important for interaction of ESCRT-III protein with VPS4. Thus, ESCRT-III proteins cycle between a closed state and an activated open state is under control of sequences at the C-terminus and associated factors.

Autoinhibitory domains (AIDs)

182-225 (C-terminal α6 domain) SS

Target domain

2-145 (N-terminal α1-α4 domains)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9SKI2

Entry ID Method Resolution Chain Position Source
AF-Q9SKI2-F1 Predicted AlphaFoldDB

13 variants for Q9SKI2

Variant ID(s) Position Change Description Diseaes Association Provenance
47 E>Q strain: cv. Ag-0 [UniProt] No
95 K>R strain: cv. Per-1 [UniProt] No
ENSVATH05295810 121 P>S No 1000Genomes
143 V>F strain: cv. Mal [UniProt] No
ENSVATH05295809 153 E>K No 1000Genomes
182 N>S strain: cv. Bas-2 [UniProt] No
ENSVATH01673392 186 G>S strain: cv. Ct-1 [UniProt] No 1000Genomes
ENSVATH05295792 192 A>T strain: cv. Tsar [UniProt] No 1000Genomes
ENSVATH05295791 193 A>T strain: cv. Edi-0, cv. Lip-0, cv. Sie and cv. Bur-0 [UniProt] No 1000Genomes
198 V>G strain: cv. Mas [UniProt] No
198 V>I strain: cv. Dul [UniProt] No
tmp_2_2588808_C_T 204 G>R No 1000Genomes
ENSVATH05295789 208 S>G No 1000Genomes

No associated diseases with Q9SKI2

9 regional properties for Q9SKI2

Type Name Position InterPro Accession
repeat Armadillo 112 - 196 IPR000225-1
repeat Armadillo 198 - 239 IPR000225-2
repeat Armadillo 242 - 281 IPR000225-3
repeat Armadillo 283 - 323 IPR000225-4
repeat Armadillo 325 - 365 IPR000225-5
repeat Armadillo 367 - 407 IPR000225-6
repeat Armadillo 410 - 450 IPR000225-7
domain Importin-alpha, importin-beta-binding domain 1 - 102 IPR002652
repeat Atypical Arm repeat 464 - 513 IPR032413

Functions

Description
EC Number
Subcellular Localization
  • Endosome
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
ESCRT III complex A complex with membrane scission activity that plays a major role in many processes where membranes are remodelled - including endosomal transport (vesicle budding), nuclear envelope organisation (membrane closure, mitotic bridge cleavage), and cytokinesis (abscission).
late endosome A prelysosomal endocytic organelle differentiated from early endosomes by lower lumenal pH and different protein composition. Late endosomes are more spherical than early endosomes and are mostly juxtanuclear, being concentrated near the microtubule organizing center.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
multivesicular body A type of endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.

No GO annotations of molecular function

Name Definition
No GO annotations for molecular function

4 GO annotations of biological process

Name Definition
endosome transport via multivesicular body sorting pathway The directed movement of substances from endosomes to lysosomes or vacuoles by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the target compartment.
intralumenal vesicle formation The invagination of the endosome membrane and resulting formation of a vesicle within the lumen of the endosome.
late endosome to vacuole transport The directed movement of substances from late endosomes to the vacuole. In yeast, after transport to the prevacuolar compartment, endocytic content is delivered to the late endosome and on to the vacuole. This pathway is analogous to endosome to lysosome transport.
protein transport The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q5ZHN1 CHMP2A Charged multivesicular body protein 2a Gallus gallus (Chicken) SS
O43633 CHMP2A Charged multivesicular body protein 2a Homo sapiens (Human) EV
Q9DB34 Chmp2a Charged multivesicular body protein 2a Mus musculus (Mouse) SS
Q8LE58 CHMP1A ESCRT-related protein CHMP1A Arabidopsis thaliana (Mouse-ear cress) PR
Q9SSM4 CHMP1B ESCRT-related protein CHMP1B Arabidopsis thaliana (Mouse-ear cress) PR
Q6DFS6 chmp2a Charged multivesicular body protein 2a Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
Q7ZW25 chmp2a Charged multivesicular body protein 2a Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MMNSIFGKRK TPAELLRENK RMLDKSIREI ERERQGLQTQ EKKLINEIKK TAKQGQMGAV
70 80 90 100 110 120
KVMAKDLIRT RHQIEKFYKL KSQLQGVSLR IQTLKSTQAM GEAMKGVTKA MGQMNRQMNL
130 140 150 160 170 180
PSLQKIMQEF ERQNEKMEMV SEVMGDAIDD ALEGDEEEEE TEDLVSQVLD EIGIDINQEL
190 200 210 220
VNAPSGAVAV PAAKNKVVQA EATGAEDSGG IDSDLQARLD NLRKM