Q9SJG9

SS
Gene name
MPK20 (At2g42880, F7D19.12)
Protein name
Mitogen-activated protein kinase 20
Names
AtMPK20 , MAP kinase 20 , EC 2.7.11.24
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT2G42880
EC number
2.7.11.24: Protein-serine/threonine kinases
Protein Class

Descriptions

MAPK1 encodes Mitogen-activated protein kinase 1, and is involved in diverse cellular responses by phosphorylating cytoplasmic and nuclear proteins. Unlike other MAP kinases, MAPK1 activates via dual phosphorylation of Thr-186 and Tyr-193 within the activation lip, and mutations at Q105 alone were sufficient for autoactivation. Additionally, in the inactive form, L76, Q10 and I87 form a structural unit that restrains intramolecular autophosphorylation by preventing interactions between activation lip residues and the catalytic base (D149) needed for phosphoryl transfer. Mutations at L76, I87 and additionally S153 increased basal specific activity via autophosphorylation of Thr-186 and Tyr-193.

Autoinhibitory domains (AIDs)

74-153 (Autoinhibitory region) SS

Target domain

25-316 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

168-194 (Activation loop from InterPro)

Target domain

25-316 (Protein kinase domain)

Relief mechanism

Assay

168-194 (Activation loop from InterPro)

Target domain

25-316 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q9SJG9

Entry ID Method Resolution Chain Position Source
AF-Q9SJG9-F1 Predicted AlphaFoldDB

30 variants for Q9SJG9

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH13622433 28 Q>R No 1000Genomes
tmp_2_17843433_T_A 43 I>L No 1000Genomes
tmp_2_17843289_T_C 91 M>V No 1000Genomes
tmp_2_17842296_A_T 245 D>E No 1000Genomes
tmp_2_17842295_G_T 246 L>M No 1000Genomes
ENSVATH07916422 270 M>L No 1000Genomes
ENSVATH05706717 280 Q>L No 1000Genomes
ENSVATH00271992 283 P>S No 1000Genomes
ENSVATH14610272 306 T>A No 1000Genomes
tmp_2_17841683_C_A 368 M>I No 1000Genomes
ENSVATH05706713 373 K>E No 1000Genomes
tmp_2_17841515_T_C 398 K>E No 1000Genomes
tmp_2_17841508_C_T 400 G>D No 1000Genomes
ENSVATH13622411 400 G>S No 1000Genomes
tmp_2_17841219_A_T 420 S>T No 1000Genomes
tmp_2_17841212_G_C 422 A>G No 1000Genomes
tmp_2_17841206_G_C 424 A>G No 1000Genomes
ENSVATH05706711 426 G>V No 1000Genomes
tmp_2_17841146_T_C 444 Q>R No 1000Genomes
ENSVATH01982154 449 N>K No 1000Genomes
ENSVATH05706710 455 A>V No 1000Genomes
ENSVATH13622410 469 M>I No 1000Genomes
tmp_2_17840918_C_T 492 R>Q No 1000Genomes
ENSVATH14610267 514 R>I No 1000Genomes
ENSVATH13622409 516 T>S No 1000Genomes
ENSVATH05706706 517 T>I No 1000Genomes
ENSVATH05706705 551 T>R No 1000Genomes
tmp_2_17840720_T_C 558 N>S No 1000Genomes
ENSVATH05706704 572 A>T No 1000Genomes
tmp_2_17840658_C_T 579 A>T No 1000Genomes

No associated diseases with Q9SJG9

3 regional properties for Q9SJG9

Type Name Position InterPro Accession
domain Protein kinase domain 20 - 270 IPR000719
binding_site Protein kinase, ATP binding site 26 - 49 IPR017441
domain Serine/threonine kinase 25, catalytic domain 15 - 291 IPR035060

Functions

Description
EC Number 2.7.11.24 Protein-serine/threonine kinases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

3 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
MAP kinase activity Catalysis of the reaction
protein serine kinase activity Catalysis of the reactions

1 GO annotations of biological process

Name Definition
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

89 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P41808 SMK1 Sporulation-specific mitogen-activated protein kinase SMK1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q00772 SLT2 Mitogen-activated protein kinase SLT2/MPK1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q3T0N5 MAPK13 Mitogen-activated protein kinase 13 Bos taurus (Bovine) SS
A5PKJ4 MAPK7 Mitogen-activated protein kinase 7 Bos taurus (Bovine) SS
P46196 MAPK1 Mitogen-activated protein kinase 1 Bos taurus (Bovine) SS
Q5F3W3 MAPK6 Mitogen-activated protein kinase 6 Gallus gallus (Chicken) SS
Q95NE7 MAPK14 Mitogen-activated protein kinase 14 Pan troglodytes (Chimpanzee) SS
Q9N272 MAPK13 Mitogen-activated protein kinase 13 Pan troglodytes (Chimpanzee) SS
Q9W354 Erk7 Extracellular signal-regulated kinase 7 Drosophila melanogaster (Fruit fly) SS
O61443 p38b Mitogen-activated protein kinase p38b Drosophila melanogaster (Fruit fly) SS
O62618 p38a Mitogen-activated protein kinase p38a Drosophila melanogaster (Fruit fly) SS
P83100 p38c Putative mitogen-activated protein kinase 14C Drosophila melanogaster (Fruit fly) SS
P40417 rl Mitogen-activated protein kinase ERK-A Drosophila melanogaster (Fruit fly) SS
O15264 MAPK13 Mitogen-activated protein kinase 13 Homo sapiens (Human) SS
P53778 MAPK12 Mitogen-activated protein kinase 12 Homo sapiens (Human) SS
Q16539 MAPK14 Mitogen-activated protein kinase 14 Homo sapiens (Human) SS
Q16659 MAPK6 Mitogen-activated protein kinase 6 Homo sapiens (Human) SS
Q15759 MAPK11 Mitogen-activated protein kinase 11 Homo sapiens (Human) SS
Q8TD08 MAPK15 Mitogen-activated protein kinase 15 Homo sapiens (Human) SS
P31152 MAPK4 Mitogen-activated protein kinase 4 Homo sapiens (Human) SS
P27361 MAPK3 Mitogen-activated protein kinase 3 Homo sapiens (Human) SS
P28482 MAPK1 Mitogen-activated protein kinase 1 Homo sapiens (Human) EV
Q9UBE8 NLK Serine/threonine-protein kinase NLK Homo sapiens (Human) SS
Q13164 MAPK7 Mitogen-activated protein kinase 7 Homo sapiens (Human) SS
Q9Z1B7 Mapk13 Mitogen-activated protein kinase 13 Mus musculus (Mouse) SS
P47811 Mapk14 Mitogen-activated protein kinase 14 Mus musculus (Mouse) SS
Q61532 Mapk6 Mitogen-activated protein kinase 6 Mus musculus (Mouse) SS
Q9WUI1 Mapk11 Mitogen-activated protein kinase 11 Mus musculus (Mouse) SS
O08911 Mapk12 Mitogen-activated protein kinase 12 Mus musculus (Mouse) SS
Q80Y86 Mapk15 Mitogen-activated protein kinase 15 Mus musculus (Mouse) SS
Q6P5G0 Mapk4 Mitogen-activated protein kinase 4 Mus musculus (Mouse) SS
P63085 Mapk1 Mitogen-activated protein kinase 1 Mus musculus (Mouse) SS
Q63844 Mapk3 Mitogen-activated protein kinase 3 Mus musculus (Mouse) SS
Q9WVS8 Mapk7 Mitogen-activated protein kinase 7 Mus musculus (Mouse) SS
P70618 Mapk14 Mitogen-activated protein kinase 14 Rattus norvegicus (Rat) SS
Q9WTY9 Mapk13 Mitogen-activated protein kinase 13 Rattus norvegicus (Rat) SS
Q9Z2A6 Mapk15 Mitogen-activated protein kinase 15 Rattus norvegicus (Rat) SS
P27704 Mapk6 Mitogen-activated protein kinase 6 Rattus norvegicus (Rat) SS
Q63538 Mapk12 Mitogen-activated protein kinase 12 Rattus norvegicus (Rat) SS
Q63454 Mapk4 Mitogen-activated protein kinase 4 Rattus norvegicus (Rat) SS
P21708 Mapk3 Mitogen-activated protein kinase 3 Rattus norvegicus (Rat) SS
P63086 Mapk1 Mitogen-activated protein kinase 1 Rattus norvegicus (Rat) SS
P0C865 Mapk7 Mitogen-activated protein kinase 7 Rattus norvegicus (Rat) SS
Q5VP69 MPK16 Mitogen-activated protein kinase 16 Oryza sativa subsp. japonica (Rice) SS
Q0E459 MPK13 Mitogen-activated protein kinase 13 Oryza sativa subsp. japonica (Rice) SS
Q6Z437 MPK3 Mitogen-activated protein kinase 3 Oryza sativa subsp. japonica (Rice) SS
Q10N20 MPK5 Mitogen-activated protein kinase 5 Oryza sativa subsp. japonica (Rice) SS
Q75KK8 MPK14 Mitogen-activated protein kinase 14 Oryza sativa subsp. japonica (Rice) SS
Q6L5F7 MPK17 Mitogen-activated protein kinase 17 Oryza sativa subsp. japonica (Rice) SS
Q84UI5 MPK1 Mitogen-activated protein kinase 1 Oryza sativa subsp. japonica (Rice) SS
Q5Z859 MPK4 Mitogen-activated protein kinase 4 Oryza sativa subsp. japonica (Rice) SS
Q5Z9J0 MPK12 Mitogen-activated protein kinase 12 Oryza sativa subsp. japonica (Rice) SS
Q5J4W4 MPK2 Mitogen-activated protein kinase 2 Oryza sativa subsp. japonica (Rice) SS
Q336X9 MPK6 Mitogen-activated protein kinase 6 Oryza sativa subsp. japonica (Rice) SS
Q53N72 MPK15 Mitogen-activated protein kinase 15 Oryza sativa subsp. japonica (Rice) SS
Q5ZCI1 MPK10 Mitogen-activated protein kinase 10 Oryza sativa subsp. japonica (Rice) SS
Q5VN19 MPK11 Mitogen-activated protein kinase 11 Oryza sativa subsp. japonica (Rice) SS
Q5SN53 MPK8 Mitogen-activated protein kinase 8 Oryza sativa subsp. japonica (Rice) SS
Q67C40 MPK7 Mitogen-activated protein kinase 7 Oryza sativa subsp. japonica (Rice) SS
Q6L5D4 MPK9 Mitogen-activated protein kinase 9 Oryza sativa subsp japonica (Rice) PR
Q17446 pmk-1 Mitogen-activated protein kinase pmk-1 Caenorhabditis elegans SS
Q11179 mapk-15 Mitogen-activated protein kinase 15 Caenorhabditis elegans SS
Q8MXI4 pmk-2 Mitogen-activated protein kinase pmk-2 Caenorhabditis elegans SS
P39745 mpk-1 Mitogen-activated protein kinase mpk-1 Caenorhabditis elegans SS
O44514 pmk-3 Mitogen-activated protein kinase pmk-3 Caenorhabditis elegans PR
G5EBT1 sma-5 Mitogen-activated protein kinase sma-5 Caenorhabditis elegans SS
O23236 MPK14 Mitogen-activated protein kinase 14 Arabidopsis thaliana (Mouse-ear cress) SS
Q39021 MPK1 Mitogen-activated protein kinase 1 Arabidopsis thaliana (Mouse-ear cress) SS
Q39022 MPK2 Mitogen-activated protein kinase 2 Arabidopsis thaliana (Mouse-ear cress) SS
Q39023 MPK3 Mitogen-activated protein kinase 3 Arabidopsis thaliana (Mouse-ear cress) SS
Q39024 MPK4 Mitogen-activated protein kinase 4 Arabidopsis thaliana (Mouse-ear cress) SS
Q39025 MPK5 Mitogen-activated protein kinase 5 Arabidopsis thaliana (Mouse-ear cress) SS
Q39026 MPK6 Mitogen-activated protein kinase 6 Arabidopsis thaliana (Mouse-ear cress) SS
Q39027 MPK7 Mitogen-activated protein kinase 7 Arabidopsis thaliana (Mouse-ear cress) SS
Q84M93 MPK17 Mitogen-activated protein kinase 17 Arabidopsis thaliana (Mouse-ear cress) SS
Q8W4J2 MPK16 Mitogen-activated protein kinase 16 Arabidopsis thaliana (Mouse-ear cress) SS
Q9C5C0 MPK18 Mitogen-activated protein kinase 18 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LM33 MPK8 Mitogen-activated protein kinase 8 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LUC3 MPK19 Mitogen-activated protein kinase 19 Arabidopsis thaliana (Mouse-ear cress) SS
Q9M1Z5 MPK10 Mitogen-activated protein kinase 10 Arabidopsis thaliana (Mouse-ear cress) SS
Q8GYQ5 MPK12 Mitogen-activated protein kinase 12 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV37 MPK9 Mitogen-activated protein kinase 9 Arabidopsis thaliana (Mouse-ear cress) SS
Q9C9U4 MPK15 Mitogen-activated protein kinase 15 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LMM5 MPK11 Mitogen-activated protein kinase 11 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LQQ9 MPK13 Mitogen-activated protein kinase 13 Arabidopsis thaliana (Mouse-ear cress) SS
P43294 MHK Serine/threonine-protein kinase MHK Arabidopsis thaliana (Mouse-ear cress) PR
O42376 mapk12 Mitogen-activated protein kinase 12 Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q9DGE1 mapk14b Mitogen-activated protein kinase 14B Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q9DGE2 mapk14a Mitogen-activated protein kinase 14A Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MQQDNRKKNN LEMEFFSDYG DANRFKVQEV IGKGSYGVVC SAIDTLTGEK VAIKKIHDIF
70 80 90 100 110 120
EHISDAARIL REIKLLRLLR HPDIVEIKHI MLPPSRREFK DIYVVFELME SDLHQVIKAN
130 140 150 160 170 180
DDLTREHYQF FLYQLLRALK YIHTANVYHR DLKPKNILAN ANCKLKICDF GLARVAFNDT
190 200 210 220 230 240
PTTIFWTDYV ATRWYRAPEL CGSFYSKYTP AIDIWSIGCI FAEVLMGKPL FPGKNVVHQL
250 260 270 280 290 300
DLMTDLLGTP SLDTISRVRN EKARRYLTSM RKKPPIPFAQ KFPNADPLSL KLLERLLAFD
310 320 330 340 350 360
PKDRPTAEEA LADPYFKGLA KVEREPSCQP ITKMEFEFER RKVTKEDIRE LISREILEYH
370 380 390 400 410 420
PQLLKDHMNG ADKASFLYPS AVDQFRRQFA HLEENSGKTG PVAPLERKHA SLPRSTVIHS
430 440 450 460 470 480
TAVARGGQPK LMNNTNTLNP ETTQNIPFNH ATIQAQQRNL SAAKPSTFMG PVAPFDNGRI
490 500 510 520 530 540
SRDAYDPRSF IRSTNLPFSQ QSAATVAMGK QQERRTTMEP EKQARQISQY NRYAPDVAIN
550 560 570 580 590 600
IDNNPFIMAR TGMNKAENIS DRIIIDTNLL QATAGIGVAA AAAAAAPGGS AHRKVGAVRY
GMSKMY