Q9SJB3

SS
Gene name
AHA5 (At2g24520, F25P17.18)
Protein name
ATPase 5, plasma membrane-type
Names
EC 7.1.2.1 , Proton pump 5
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT2G24520
EC number
7.1.2.1: Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Protein Class

Descriptions

The plasma membrane H+-ATPase belongs to the superfamily of P-type ATPases. The H+-ATPase is responsible for the ATP-fuelled ejection of protons from the cell and is an important component in the generation of the trans-membrane H+ gradient in plant cells. The H+-ATPase AHA2 is controlled by an autoinhibitory C-terminal regulatory domain and activated by 14-3-3 proteins which bind to the part of the enzyme. In two regions of the C-terminus, alteration of the residues leads to increased pump activity. One region stretches from K863 to L885 and includes two residues (Q879 and R880) that are conserved between plant and fungal H+-ATPases. The other region, incorporating S904 to L919, is situated in an extension of the C-terminus unique to plant H+-ATPases.

Autoinhibitory domains (AIDs)

863-885 (Autoinhibitory domain I) SS

Target domain

309-537 (ATPase domain)

Relief mechanism

Partner binding

Assay

904-919 (Autoinhibitory domain II) SS

Target domain

309-537 (ATPase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9SJB3

Entry ID Method Resolution Chain Position Source
AF-Q9SJB3-F1 Predicted AlphaFoldDB

No variants for Q9SJB3

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9SJB3

No associated diseases with Q9SJB3

3 regional properties for Q9SJB3

Type Name Position InterPro Accession
domain Cation-transporting P-type ATPase, N-terminal 13 - 85 IPR004014
ptm P-type ATPase, phosphorylation site 329 - 335 IPR018303
domain P-type ATPase, haloacid dehalogenase domain 309 - 637 IPR044492

Functions

Description
EC Number 7.1.2.1 Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Subcellular Localization
  • Membrane; Multi-pass membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
P-type proton-exporting transporter activity Enables the transfer of protons from one side of a membrane to the other according to the reaction

2 GO annotations of biological process

Name Definition
proton export across plasma membrane The directed movement of hydrogen ions (protons) from inside a cell, across the plasma membrane and into the extracellular region.
regulation of intracellular pH Any process that modulates the internal pH of a cell, measured by the concentration of the hydrogen ion.

13 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P83970 ha1 Plasma membrane ATPase Triticum aestivum (Wheat) SS
Q7XPY2 Os04g0656100 Plasma membrane ATPase Oryza sativa subsp. japonica (Rice) SS
P20431 AHA3 ATPase 3, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P20649 AHA1 ATPase 1, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q43128 AHA10 ATPase 10, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV11 AHA11 ATPase 11, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LY32 AHA7 ATPase 7, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2A0 AHA8 ATPase 8, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SH76 AHA6 ATPase 6, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SU58 AHA4 ATPase 4, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P19456 AHA2 ATPase 2, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) EV
Q42556 AHA9 ATPase 9, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P22180 LHA1 Plasma membrane ATPase 1 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MSELDHIKNE SVDLVRIPME EVFEELKCTK QGLTANEASH RLDVFGPNKL EEKKESKLLK
70 80 90 100 110 120
FLGFMWNPLS WVMEVAALMA IALANGGGRP PDWQDFVGIV CLLLINSTIS FIEENNAGNA
130 140 150 160 170 180
AAALMAGLAP KTKVLRDNQW SEQEASILVP GDVISIKLGD IIPADARLLD GDPLKIDQSS
190 200 210 220 230 240
LTGESIPVTK NPSDEVFSGS ICKQGEIEAI VIATGVHTFF GKAAHLVDNT NQIGHFQKVL
250 260 270 280 290 300
TSIGNFCICS IALGIIVELL VMYPIQRRRY RDGIDNLLVL LIGGIPIAMP SVLSVTMATG
310 320 330 340 350 360
SHRLFQQGAI TKRMTAIEEM AGMDVLCCDK TGTLTLNKLT VDKNLVEVFA KGVGKEHVFL
370 380 390 400 410 420
LAARASRIEN QDAIDAAIVG MLADPKEARA GVREVHFFPF NPVDKRTALT YVDSDGNWHR
430 440 450 460 470 480
ASKGAPEQIL NLCNCKEDVR RKVHGVIDKF AERGLRSLAV ARQEVLEKKK DAPGGPWQLV
490 500 510 520 530 540
GLLPLFDPPR HDSAETIRRA LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSSALLGQ
550 560 570 580 590 600
VKDSSLGALP VDELIEKADG FAGVFPEHKY EIVHRLQQRN HICGMTGDGV NDAPALKKAD
610 620 630 640 650 660
IGIAVVDATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV SITIRIVFGF
670 680 690 700 710 720
MFIALIWQFD FSPFMVLIIA ILNDGTIMTI SKDRMKPSPQ PDSWKLRDIF STGVVLGGYQ
730 740 750 760 770 780
ALMTVVFFWV MKDSDFFSNY FGVRPLSQRP EQMMAALYLQ VSIISQALIF VTRSRSWSYA
790 800 810 820 830 840
ECPGLLLLGA FVIAQLVATF IAVYANWSFA RIEGAGWGWA GVIWLYSFLT YIPLDLLKFG
850 860 870 880 890 900
IRYVLSGKAW LNLLENKTAF TTKKDYGKEE REAQWAAAQR TLHGLQPAEK NNIFNEKNSY
910 920 930 940
SELSQIAEQA KRRAEVVRLR EINTLKGHVE SVVKLKGLDI DTIQQHYTV