Q9QUG9
Gene name |
Rasgrp2 |
Protein name |
RAS guanyl-releasing protein 2 |
Names |
Calcium and DAG-regulated guanine nucleotide exchange factor I , CalDAG-GEFI , F25B3.3 kinase-like protein |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:19395 |
EC number |
|
Protein Class |
|
Descriptions
Autoinhibitory domains (AIDs)
Target domain |
150-388 (Ras guanine-nucleotide exchange factors catalytic domain) |
Relief mechanism |
Ligand binding, PTM |
Assay |
|
Target domain |
419-463 (EF domain) |
Relief mechanism |
Ligand binding, PTM |
Assay |
|
Accessory elements
No accessory elements
References
- Czikora A et al. (2016) "Structural Basis for the Failure of the C1 Domain of Ras Guanine Nucleotide Releasing Protein 2 (RasGRP2) to Bind Phorbol Ester with High Affinity", The Journal of biological chemistry, 291, 11133-47
- Canault M et al. (2020) "RasGRP2 Structure, Function and Genetic Variants in Platelet Pathophysiology", International journal of molecular sciences, 21,
- Iwig JS et al. (2013) "Structural analysis of autoinhibition in the Ras-specific exchange factor RasGRP1", eLife, 2, e00813
- Ren J et al. (2016) "A negative-feedback loop regulating ERK1/2 activation and mediated by RasGPR2 phosphorylation", Biochemical and biophysical research communications, 474, 193-198
Autoinhibited structure
Activated structure
1 structures for Q9QUG9
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9QUG9-F1 | Predicted | AlphaFoldDB |
40 variants for Q9QUG9
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3389514284 | 26 | D>E | No | EVA | |
| rs3389501202 | 31 | R>L | No | EVA | |
| rs3389526741 | 34 | Q>H | No | EVA | |
| rs3389536765 | 93 | N>K | No | EVA | |
| rs3389531155 | 95 | E>* | No | EVA | |
| rs3389514952 | 100 | I>V | No | EVA | |
| rs3389478196 | 102 | E>G | No | EVA | |
| rs3389514927 | 109 | Q>E | No | EVA | |
| rs3389514935 | 154 | E>K | No | EVA | |
| rs3389501188 | 163 | T>I | No | EVA | |
| rs3389533448 | 201 | N>D | No | EVA | |
| rs3389521938 | 231 | A>T | No | EVA | |
| rs3407830269 | 233 | R>K | No | EVA | |
| rs3389536796 | 299 | F>I | No | EVA | |
| rs3389514964 | 312 | V>M | No | EVA | |
| rs3389514244 | 343 | E>D | No | EVA | |
| rs3389514288 | 357 | N>S | No | EVA | |
| rs3389514300 | 359 | D>H | No | EVA | |
| rs3389501275 | 387 | R>C | No | EVA | |
| rs3389478133 | 394 | S>I | No | EVA | |
| rs3389533483 | 395 | P>L | No | EVA | |
| rs3554101415 | 396 | T>A | No | EVA | |
| rs3389514961 | 398 | C>S | No | EVA | |
| rs3389521918 | 399 | T>S | No | EVA | |
| rs3389478164 | 400 | P>L | No | EVA | |
| rs3389526755 | 443 | D>N | No | EVA | |
| rs3389536711 | 447 | S>T | No | EVA | |
| rs3389488071 | 462 | S>N | No | EVA | |
| rs3389514254 | 479 | E>D | No | EVA | |
| rs3389501186 | 518 | L>P | No | EVA | |
| rs3389501205 | 533 | G>R | No | EVA | |
| rs3389501229 | 545 | S>L | No | EVA | |
| rs3389521940 | 554 | S>G | No | EVA | |
| rs387318325 | 562 | P>S | No | EVA | |
| rs3389517048 | 569 | T>I | No | EVA | |
| rs3409011441 | 572 | R>Q | No | EVA | |
| rs3408933005 | 580 | R>G | No | EVA | |
| rs3408829784 | 581 | P>R | No | EVA | |
| rs3407830282 | 584 | R>H | No | EVA | |
| rs260219837 | 598 | S>T | No | EVA |
No associated diseases with Q9QUG9
6 regional properties for Q9QUG9
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Ras-like guanine nucleotide exchange factor, N-terminal | 3 - 126 | IPR000651 |
| domain | Ras guanine-nucleotide exchange factors catalytic domain | 150 - 388 | IPR001895 |
| domain | EF-hand domain | 426 - 487 | IPR002048 |
| domain | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain | 498 - 550 | IPR002219 |
| binding_site | EF-Hand 1, calcium-binding site | 439 - 451 | IPR018247-1 |
| binding_site | EF-Hand 1, calcium-binding site | 468 - 480 | IPR018247-2 |
Functions
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| neuron projection | A prolongation or process extending from a nerve cell, e.g. an axon or dendrite. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| ruffle membrane | The portion of the plasma membrane surrounding a ruffle. |
| synapse | The junction between an axon of one neuron and a dendrite of another neuron, a muscle fiber or a glial cell. As the axon approaches the synapse it enlarges into a specialized structure, the presynaptic terminal bouton, which contains mitochondria and synaptic vesicles. At the tip of the terminal bouton is the presynaptic membrane; facing it, and separated from it by a minute cleft (the synaptic cleft) is a specialized area of membrane on the receiving cell, known as the postsynaptic membrane. In response to the arrival of nerve impulses, the presynaptic terminal bouton secretes molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| calcium ion binding | Binding to a calcium ion (Ca2+). |
| guanyl-nucleotide exchange factor activity | Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions. |
2 GO annotations of biological process
| Name | Definition |
|---|---|
| cellular response to calcium ion | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a calcium ion stimulus. |
| Ras protein signal transduction | The series of molecular signals within the cell that are mediated by a member of the Ras superfamily of proteins switching to a GTP-bound active state. |
28 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q1LZ97 | RASGRP4 | RAS guanyl-releasing protein 4 | Bos taurus (Bovine) | SS |
| A6N9I4 | RASGRP2 | RAS guanyl-releasing protein 2 | Bos taurus (Bovine) | SS |
| A0A3S5ZPR1 | RASGRP3 | RAS guanyl releasing protein 3 | Gallus gallus (Chicken) | SS |
| P26675 | Sos | Protein son of sevenless | Drosophila melanogaster (Fruit fly) | SS |
| Q07890 | SOS2 | Son of sevenless homolog 2 | Homo sapiens (Human) | SS |
| Q5JS13 | RALGPS1 | Ras-specific guanine nucleotide-releasing factor RalGPS1 | Homo sapiens (Human) | PR |
| Q86X27 | RALGPS2 | Ras-specific guanine nucleotide-releasing factor RalGPS2 | Homo sapiens (Human) | PR |
| Q13905 | RAPGEF1 | Rap guanine nucleotide exchange factor 1 | Homo sapiens (Human) | PR |
| Q07889 | SOS1 | Son of sevenless homolog 1 | Homo sapiens (Human) | EV |
| Q8TDF6 | RASGRP4 | RAS guanyl-releasing protein 4 | Homo sapiens (Human) | SS |
| O95267 | RASGRP1 | RAS guanyl-releasing protein 1 | Homo sapiens (Human) | EV |
| Q8IV61 | RASGRP3 | Ras guanyl-releasing protein 3 | Homo sapiens (Human) | SS |
| Q7LDG7 | RASGRP2 | RAS guanyl-releasing protein 2 | Homo sapiens (Human) | EV SS |
| Q02384 | Sos2 | Son of sevenless homolog 2 | Mus musculus (Mouse) | SS |
| Q62245 | Sos1 | Son of sevenless homolog 1 | Mus musculus (Mouse) | SS |
| Q8VCC8 | Rapgef3 | Rap guanine nucleotide exchange factor 3 | Mus musculus (Mouse) | SS |
| Q9EQZ6 | Rapgef4 | Rap guanine nucleotide exchange factor 4 | Mus musculus (Mouse) | EV |
| Q9D300 | Rasgef1c | Ras-GEF domain-containing family member 1C | Mus musculus (Mouse) | PR |
| Q03385 | Ralgds | Ral guanine nucleotide dissociation stimulator | Mus musculus (Mouse) | PR |
| Q9Z1S3 | Rasgrp1 | RAS guanyl-releasing protein 1 | Mus musculus (Mouse) | SS |
| Q8BTM9 | Rasgrp4 | RAS guanyl-releasing protein 4 | Mus musculus (Mouse) | SS |
| Q9ERD6 | Ralgps2 | Ras-specific guanine nucleotide-releasing factor RalGPS2 | Mus musculus (Mouse) | PR |
| A2AR50 | Ralgps1 | Ras-specific guanine nucleotide-releasing factor RalGPS1 | Mus musculus (Mouse) | PR |
| Q8R5I4 | Rasgrp4 | RAS guanyl-releasing protein 4 | Rattus norvegicus (Rat) | SS |
| Q9R1K8 | Rasgrp1 | RAS guanyl-releasing protein 1 | Rattus norvegicus (Rat) | SS |
| P0C643 | Rasgrp2 | RAS guanyl-releasing protein 2 | Rattus norvegicus (Rat) | SS |
| Q9N5D3 | sos-1 | Son of sevenless homolog | Caenorhabditis elegans | EV |
| A4IJ06 | rasgrp1 | RAS guanyl-releasing protein 1 | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MTSTLDLDKG | CTVEELLRGC | IEAFDDSGKV | RDPQLVRMFL | MMHPWYIPSS | QLASKLLHFY |
| 70 | 80 | 90 | 100 | 110 | 120 |
| QQSRKDNSNS | LQMKTCHLVR | YWISAFPAEF | DLNPELAEQI | KELKALLDQE | GNRRHSSLID |
| 130 | 140 | 150 | 160 | 170 | 180 |
| IESVPTYKWK | RQVTQRNPVE | QKKRKMSLLF | DHLEPMELAE | HLTYLEYRSF | CKILFQDYHS |
| 190 | 200 | 210 | 220 | 230 | 240 |
| FVTHGCTVDN | PVLERFISLF | NSVSQWVQLM | ILSKPTATQR | ALVITHFVHV | AERLLQLQNF |
| 250 | 260 | 270 | 280 | 290 | 300 |
| NTLMAVVGGL | SHSSISRLKE | THSHVSPDTI | KLWEGLTELV | TATGNYSNYR | RRLAACVGFR |
| 310 | 320 | 330 | 340 | 350 | 360 |
| FPILGVHLKD | LVALQLALPD | WLDPGRTRLN | GAKMRQLFCI | LEELAMVTSL | RPPVQANPDL |
| 370 | 380 | 390 | 400 | 410 | 420 |
| LSLLTVSLDQ | YQTEDELYQL | SLQREPRSKS | SPTSPTSCTP | PPRPPVLEEW | TSVAKPKLDQ |
| 430 | 440 | 450 | 460 | 470 | 480 |
| ALVAEHIEKM | VESVFRNFDV | DGDGHISQEE | FQIIRGNFPY | LSAFGDLDQN | QDGCISREEM |
| 490 | 500 | 510 | 520 | 530 | 540 |
| ISYFLRSSSV | LGGRMGFVHN | FQESNSLRPV | ACRHCKALIL | GIYKQGLKCR | ACGVNCHKQC |
| 550 | 560 | 570 | 580 | 590 | 600 |
| KERLSVECRR | RAQSVSLEGS | APSPSPTHTH | HRAFSFSLPR | PGRRSSRPPE | IREEEVQSVE |
| DGVFDIHL |