Q9NBD7

SS
Gene name
chb (CLASP, Mast, MESR7, orbit, CG32435)
Protein name
CLIP-associating protein
Names
Misexpression suppressor of ras 7 , Protein Multiple asters , Mast , Protein Orbit , Protein chromosome bows
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG32435
EC number
Protein Class

Descriptions

CLASPs, major microtubule-stabilizing factors in interphase and mitosis, prevent microtubule from switching form growth to shortening by stabilizing growing microtubule ends, and thus suppress microtubule catastrophes. CLASP2a consists of three TOG-like domains (termed TOG1, 2, and 3) and a C-terminal domain, CLIP-interacting domain (CLIP-ID) responsible for interaction with CLIP-170 and other partners. CLASP2a TOG2 is necessary and sufficient for catastrophe inhibition. The inhibition function is suppressed by the C-terminal domain CLIP-ID while the TOG1 domain or the CLIP-ID partner binding to CLIP-ID can release the autoinhibition.

Autoinhibitory domains (AIDs)

1370-1480 (C-terminal domain CLIP-ID) SS

Target domain

834-1078 (TOG2 domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for Q9NBD7

Entry ID Method Resolution Chain Position Source
4G3A X-ray 199 A A/B 1-229 PDB
AF-Q9NBD7-F1 Predicted AlphaFoldDB

No variants for Q9NBD7

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9NBD7

No associated diseases with Q9NBD7

8 regional properties for Q9NBD7

Type Name Position InterPro Accession
repeat HEAT, type 2 169 - 207 IPR021133-1
repeat HEAT, type 2 1414 - 1452 IPR021133-2
domain CLASP N-terminal domain 316 - 536 IPR024395
domain TOG domain 2 - 234 IPR034085-1
domain TOG domain 312 - 548 IPR034085-2
domain TOG domain 836 - 1072 IPR034085-3
domain TOG domain 1251 - 1477 IPR034085-4
domain XMAP215/Dis1/CLASP, TOG domain 66 - 204 IPR048491

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton
  • Nucleus
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Cytoplasm, cytoskeleton, spindle
  • Cell projection, growth cone
  • Cleavage furrow
  • Localizes to punctate cytoplasmic foci in interphase
  • Concentrates on spindle microtubules from prophase to late anaphase
  • Accumulates on the mid-part of the central spindle during telophase where it remains until cleavage
  • Subsequently appears at the periphery of the reforming karyomeres
  • Also localizes to the spindle remnant, cleavage furrows and the fusome of the 16-cell ovarian cyst
  • Localizes to the axonal growth cone within neurons
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

24 GO annotations of cellular component

Name Definition
basal cortex The region that lies just beneath the plasma membrane on the basal edge of a cell.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
chromosome, centromeric region The region of a chromosome that includes the centromeric DNA and associated proteins. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome.
cilium A specialized eukaryotic organelle that consists of a filiform extrusion of the cell surface and of some cytoplasmic parts. Each cilium is largely bounded by an extrusion of the cytoplasmic (plasma) membrane, and contains a regular longitudinal array of microtubules, anchored to a basal body.
cleavage furrow The cleavage furrow is a plasma membrane invagination at the cell division site. The cleavage furrow begins as a shallow groove and eventually deepens to divide the cytoplasm.
contractile ring A cytoskeletal structure composed of filamentous protein that forms beneath the membrane of many cells or organelles, in the plane of cell or organelle division. Ring contraction is associated with centripetal growth of the membrane that divides the cytoplasm of the two daughter cells or organelles.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic microtubule Any microtubule in the cytoplasm of a cell.
fusome A large intracellular spectrin-rich structure that has been found in insect germline cells and mammalian hematopoietic cells. The fusome is an elongated, branched structure, formed from the spherical spectrosome organelle.
germline ring canal Germline specific intercellular bridge. During cyst formation in insects, ring canals interconnect the cells of the cyst, facilitating the passage of cytoplasmic components between cells.
growth cone The migrating motile tip of a growing neuron projection, where actin accumulates, and the actin cytoskeleton is the most dynamic.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
microtubule associated complex Any multimeric complex connected to a microtubule.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
microtubule plus-end The growing (plus) end of a microtubule. In vitro, microtubules polymerize more quickly at the plus end than at the minus end. In vivo, microtubule growth occurs only at the plus end, and the plus end switches between periods of growth and shortening, a behavior known as dynamic instability.
mitotic spindle A spindle that forms as part of mitosis. Mitotic and meiotic spindles contain distinctive complements of proteins associated with microtubules.
nuclear membrane Either of the lipid bilayers that surround the nucleus and form the nuclear envelope; excludes the intermembrane space.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
polar microtubule Any of the spindle microtubules that come from each pole and overlap at the spindle midzone. This interdigitating structure consisting of antiparallel microtubules is responsible for pushing the poles of the spindle apart.
spectrosome A germline specific spherical organelle, rich in membrane skeletal proteins. Precursor to the fusome.
spindle The array of microtubules and associated molecules that forms between opposite poles of a eukaryotic cell during mitosis or meiosis and serves to move the duplicated chromosomes apart.
spindle envelope An organelle envelope that surrounds the chromosomes and the central part of the spindle apparatus during mitosis and meiosis; observed in many invertebrates. The spindle envelope consists of membrane layers, called parafusorial membranes, derived from endoplasmic reticulum membrane; in male meiosis it forms during prometaphase and persists until early in the ensuing interphase.
spindle microtubule Any microtubule that is part of a mitotic or meiotic spindle; anchored at one spindle pole.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

2 GO annotations of molecular function

Name Definition
GTP binding Binding to GTP, guanosine triphosphate.
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.

18 GO annotations of biological process

Name Definition
attachment of mitotic spindle microtubules to kinetochore The cell cycle process in which spindle microtubules become physically associated with the proteins making up the kinetochore complex as part of mitotic metaphase plate congression.
axon guidance The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues.
cystoblast division Any of the rounds of incomplete mitosis undergone by a cystoblast to form a cyst of interconnected cells.
establishment of mitotic spindle localization The cell cycle process in which the directed movement of the mitotic spindle to a specific location in the cell occurs.
hemocyte migration The directed movement of a hemocyte within the embryo. Hemocytes are blood cells associated with a hemocoel (the cavity containing most of the major organs of the arthropod body) which are involved in defense and clotting of hemolymph, but not involved in transport of oxygen. In Drosophila, embryonic hemocytes originate from the head mesoderm as a cluster of cells. The cluster splits into two and one group of cells crosses the amnioserosa. Both populations then spread toward the middle of the embryo and then disperse evenly throughout the embryo.
mitotic cell cycle Progression through the phases of the mitotic cell cycle, the most common eukaryotic cell cycle, which canonically comprises four successive phases called G1, S, G2, and M and includes replication of the genome and the subsequent segregation of chromosomes into daughter cells. In some variant cell cycles nuclear replication or nuclear division may not be followed by cell division, or G1 and G2 phases may be absent.
mitotic sister chromatid segregation The cell cycle process in which replicated homologous chromosomes are organized and then physically separated and apportioned to two sets during the mitotic cell cycle. Each replicated chromosome, composed of two sister chromatids, aligns at the cell equator, paired with its homologous partner. One homolog of each morphologic type goes into each of the resulting chromosome sets.
mitotic spindle assembly Mitotic bipolar spindle assembly begins with spindle microtubule nucleation from the separated spindle pole body, includes spindle elongation during prometaphase, and is complete when all kinetochores are stably attached the spindle, and the spindle assembly checkpoint is satisfied.
mitotic spindle elongation The cell cycle process in which the distance is lengthened between poles of the mitotic spindle. Mitotic spindle elongation begins during mitotic prophase and ends during mitotic anaphase B.
mitotic spindle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
oocyte microtubule cytoskeleton organization Formation and maintenance of a polarized microtubule array originating from a microtubule-organizing center (MTOC) in the oocyte. An example of this is found in Drosophila melanogaster.
oogenesis The complete process of formation and maturation of an ovum or female gamete from a primordial female germ cell. Examples of this process are found in Mus musculus and Drosophila melanogaster.
ovarian fusome organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the fusome of ovarian cells, an organelle derived from the spectrosome. It anchors the mitotic spindle pole to provide orientation during cystoblast cell divisions.
positive regulation of microtubule polymerization Any process that activates or increases the frequency, rate or extent of microtubule polymerization.
regulation of mitotic centrosome separation Any process that modulates the frequency, rate or extent of the separation of duplicated centrosome components at the beginning of mitosis.
spindle assembly The aggregation, arrangement and bonding together of a set of components to form the spindle, the array of microtubules and associated molecules that serves to move duplicated chromosomes apart.
spindle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the spindle, the array of microtubules and associated molecules that forms between opposite poles of a eukaryotic cell during DNA segregation and serves to move the duplicated chromosomes apart.
stem cell population maintenance The process by which an organism or tissue maintains a population of stem cells of a single type. This can be achieved by a number of mechanisms

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P38198 STU1 Protein STU1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q7Z460 CLASP1 CLIP-associating protein 1 Homo sapiens (Human) SS
O75122 CLASP2 CLIP-associating protein 2 Homo sapiens (Human) EV
Q80TV8 Clasp1 CLIP-associating protein 1 Mus musculus (Mouse) SS
Q8BRT1 Clasp2 CLIP-associating protein 2 Mus musculus (Mouse) SS
Q99JD4 Clasp2 CLIP-associating protein 2 Rattus norvegicus (Rat) SS
Q6NYW6 clasp2 CLIP-associating protein 2 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MAYRKPSDLD GFIQQMPKAD MRVKVQLAED LVTFLSDDTN SIVCTDMGFL IDGLMPWLTG
70 80 90 100 110 120
SHFKIAQKSL EAFSELIKRL GSDFNAYTAT VLPHVIDRLG DSRDTVREKA QLLLRDLMEH
130 140 150 160 170 180
RVLPPQALID KLATSCFKHK NAKVREEFLQ TIVNALHEYG TQQLSVRVYI PPVCALLGDP
190 200 210 220 230 240
TVNVREAAIQ TLVEIYKHVG DRLRPDLRRM DDVPASKLAM LEQKFDQVKQ EGLLLPSALK
250 260 270 280 290 300
NTNGNGVGLD EADNIGLRER PTRMIKRPLH SAVSSSLRPK PNVNDVTGDA GAVTMESFES
310 320 330 340 350 360
SFEVVPQLNI FHAKDMDDIY KQVLVIISDK NADWEKRVDA LKKIRALLIL SYHTQPQFVA
370 380 390 400 410 420
VQLKELSLSF VDILKEELRS QVIREACITI AYMSKTLRNK LDAFCWSILE HLINLIQNSA
430 440 450 460 470 480
KVIASASTIA LKYIIKYTHA PKLLKIYTDT LNQSKSKDIR STLCELMVLL FEEWQTKALE
490 500 510 520 530 540
RNATVLRDTL KKSIGDADCD ARRHSRYAYW AFRRHFPELA DQIYGTLDIA AQRALERERE
550 560 570 580 590 600
GGGGGGTGTG TGTAPETRRT VSRIGRTPGT LQKPTPSMRS ISAVDTAAAQ RAKVRAQYTL
610 620 630 640 650 660
YSRQRKPLGP NNSNQASMTG AAASGSLPRP RLNSNSGGTP ATTPGSVTPR PRGRAGVSQS
670 680 690 700 710 720
QPGSRSTSPS TKLRDQYGGI GNYYRGATGA IPKKASGIPR STASSRETSP TRSGGGLMKR
730 740 750 760 770 780
SMYSTGAGSR RTPERNNPVR PSAAARLLAQ SREAEHTLGV GDDGQPDYVS GDYMRSGGMR
790 800 810 820 830 840
MGRKLMGRDE SDDIDSEASS VCSERSFDSS YTRGNKSNYS LSGSHTRLDW STQRAPFDDI
850 860 870 880 890 900
ETIIQFCAST HWSERKDGLI SLTQYLADGK ELTQQQLKCV LDMFRKMFMD THTKVYSLFL
910 920 930 940 950 960
DTVTELILVH ANELHEWLFI LLTRLFNKLG TDLLNSMHSK IWKTLQVVHE YFPTQLQLKE
970 980 990 1000 1010 1020
LFRIISDSTQ TPTTKTRIAI LRFLTDLANT YCKSSDFPSD QSQACERTVL KLAQLAADQK
1030 1040 1050 1060 1070 1080
SMELRSQARS CLVALYNLNT PQMTLLLADL PKVYQDSARS CIHSHMRRQS QSCNSGANSP
1090 1100 1110 1120 1130 1140
SSSPLSSSSP KPLQSPSVGP FASLQSHHHQ LSISSTSPRS RQSSVEQELL FSSELDIQHN
1150 1160 1170 1180 1190 1200
IQKTSEEIRH CFGGQYQTAL APNGFNGHLQ YHDQGQQDSC ASLSSNSKTQ SSANTTQSNT
1210 1220 1230 1240 1250 1260
PESATMRLDN LERERTTQNA KSPTDDAKVI TVSINMAENG ELILASNLME SEVVRVALTL
1270 1280 1290 1300 1310 1320
TKDQPVELLQ TSLTNLGICI KGGNCELPNK HFRSIMRMLL NILEAEHTDV VIAGLHVLSK
1330 1340 1350 1360 1370 1380
IMRSNKMRHN WMHFLELILL KIIQCYQHSK EALRDIDSMI PRIAPSLPLD LSINIVNPVI
1390 1400 1410 1420 1430 1440
ATGEFPTNLC AIKILLEVTE HHGSEITDAH LDIVFPNLAR SADDTQSMVR KAAVFCIVKL
1450 1460 1470 1480 1490
YFVLGEEKVK PKLSVLNPSK VRLLNVYIEK QRNCISGGGS STKNSSAASS S