Q9N2V6

SS
Gene name
gpa-16 (spn-1, Y95B8A.5)
Protein name
Guanine nucleotide-binding protein alpha-16 subunit
Names
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_Y95B8A.5
EC number
Protein Class

Descriptions

The Gα subunits of heterotrimeric G proteins are GTP hydrolyases which, upon receptor activation, bind to GTP and regulate effector molecules. Gα-catalyzed hydrolysis of GTP to GDP releases the Gα-GDP product complex from effector and allows its sequestration by inhibitory Gβγ subunits. In Gia1-GppNHp complex, Gln204 of Gia1 interacts with nucleophilic water (Wnuc), participating directly in the Gia1-GTP ground state but must reorient to stabilize the transition state. RGS4, a member of the RGS family of G protein stimulatory factors, accelerates hydrolysis of GTP by Gia1 by destabilizing the ground-state conformation of Gln204. Insertion of Asn128 of RGS4 into the active site enhances catalysis by aiding in binding, orienting, and polarizing Wnuc in the pre-transition state complex. Asn128 of RGS4 displaces the side chain of Gln204 from its anti-catalytic position in the ground state, freeing it to participate in stabilization of the transition state.

Autoinhibitory domains (AIDs)

204-205 (Gln204) SS

Target domain

179-352 (P-loop containing nucleoside triphosphate hydrolase domain)

Relief mechanism

Partner binding

Assay

62-177 (α-H domain) SS

Target domain

184-351 (Ras-like domain)

Relief mechanism

Ligand binding, Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9N2V6

Entry ID Method Resolution Chain Position Source
AF-Q9N2V6-F1 Predicted AlphaFoldDB

No variants for Q9N2V6

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9N2V6

No associated diseases with Q9N2V6

3 regional properties for Q9N2V6

Type Name Position InterPro Accession
domain Protein kinase domain 444 - 794 IPR000719
active_site Serine/threonine-protein kinase, active site 575 - 587 IPR008271
binding_site Protein kinase, ATP binding site 450 - 473 IPR017441

Functions

Description
EC Number
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
heterotrimeric G-protein complex Any of a family of heterotrimeric GTP-binding and hydrolyzing proteins; they belong to a superfamily of GTPases that includes monomeric proteins such as EF-Tu and RAS. Heterotrimeric G-proteins consist of three subunits; the alpha subunit contains the guanine nucleotide binding site and possesses GTPase activity; the beta and gamma subunits are tightly associated and function as a beta-gamma heterodimer; extrinsic plasma membrane proteins (cytoplasmic face) that function as a complex to transduce signals from G protein-coupled receptors to an effector protein.

6 GO annotations of molecular function

Name Definition
G protein-coupled receptor binding Binding to a G protein-coupled receptor.
G-protein beta/gamma-subunit complex binding Binding to a complex of G-protein beta/gamma subunits.
GDP binding Binding to GDP, guanosine 5'-diphosphate.
GTP binding Binding to GTP, guanosine triphosphate.
GTPase activity Catalysis of the reaction
metal ion binding Binding to a metal ion.

4 GO annotations of biological process

Name Definition
adenylate cyclase-modulating G protein-coupled receptor signaling pathway A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation or inhibition of adenylyl cyclase activity and a subsequent change in the intracellular concentration of cyclic AMP (cAMP).
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
embryonic axis specification The establishment, maintenance and elaboration of a pattern along a line or a point in an embryo.
establishment of mitotic spindle orientation A cell cycle process that sets the alignment of mitotic spindle relative to other cellular structures.

58 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P0C7Q4 GNAT3 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P04696 GNAT2 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P04695 GNAT1 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P08239 GNAO1 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P63097 GNAI1 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P50147 GNAI2 Guanine nucleotide-binding protein G Gallus gallus (Chicken) SS
P50146 GNAI1 Guanine nucleotide-binding protein G Gallus gallus (Chicken) SS
P38400 GNAI2 Guanine nucleotide-binding protein G(i) subunit alpha-2 Canis lupus familiaris (Dog) (Canis familiaris) PR
P16378 Galphao G protein alpha o subunit Drosophila melanogaster (Fruit fly) SS
P25157 cta Guanine nucleotide-binding protein subunit alpha homolog Drosophila melanogaster (Fruit fly) SS
P20353 Galphai G protein alpha i subunit Drosophila melanogaster (Fruit fly) SS
P11488 GNAT1 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
P19087 GNAT2 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
P09471 GNAO1 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
Q03113 GNA12 Guanine nucleotide-binding protein subunit alpha-12 Homo sapiens (Human) SS
A8MTJ3 GNAT3 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
Q14344 GNA13 Guanine nucleotide-binding protein subunit alpha-13 Homo sapiens (Human) SS
P19086 GNAZ Guanine nucleotide-binding protein G Homo sapiens (Human) SS
P04899 GNAI2 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
P08754 GNAI3 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
P63096 GNAI1 Guanine nucleotide-binding protein G Homo sapiens (Human) EV
P27600 Gna12 Guanine nucleotide-binding protein subunit alpha-12 Mus musculus (Mouse) SS
P20612 Gnat1 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P27601 Gna13 Guanine nucleotide-binding protein subunit alpha-13 Mus musculus (Mouse) SS
Q3V3I2 Gnat3 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
O70443 Gnaz Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P50149 Gnat2 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P18872 Gnao1 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
B2RSH2 Gnai1 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P08752 Gnai2 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
Q9DC51 Gnai3 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P93564 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Solanum tuberosum (Potato) SS
Q63210 Gna12 Guanine nucleotide-binding protein subunit alpha-12 Rattus norvegicus (Rat) SS
P19627 Gnaz Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P29348 Gnat3 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P59215 Gnao1 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
Q6Q7Y5 Gna13 Guanine nucleotide-binding protein subunit alpha-13 Rattus norvegicus (Rat) SS
P04897 Gnai2 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P08753 Gnai3 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P10824 Gnai1 Guanine nucleotide-binding protein G subunit alpha-1 Rattus norvegicus (Rat) EV
Q0DJ33 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Oryza sativa subsp. japonica (Rice) SS
P28051 gpa-1 Guanine nucleotide-binding protein alpha-1 subunit Caenorhabditis elegans SS
P28052 gpa-3 Guanine nucleotide-binding protein alpha-3 subunit Caenorhabditis elegans SS
P51875 goa-1 Guanine nucleotide-binding protein G Caenorhabditis elegans SS
P91907 gpa-15 Guanine nucleotide-binding protein alpha-15 subunit Caenorhabditis elegans SS
Q18434 odr-3 Guanine nucleotide-binding protein alpha-17 subunit Caenorhabditis elegans SS
Q20907 gpa-8 Guanine nucleotide-binding protein alpha-8 subunit Caenorhabditis elegans PR
Q21917 gpa-7 Guanine nucleotide-binding protein alpha-7 subunit Caenorhabditis elegans SS
Q93743 gpa-6 Guanine nucleotide-binding protein alpha-6 subunit Caenorhabditis elegans SS
Q9BIG4 gpa-10 Guanine nucleotide-binding protein alpha-10 subunit Caenorhabditis elegans PR
Q9BIG5 gpa-4 Guanine nucleotide-binding protein alpha-4 subunit Caenorhabditis elegans SS
Q9XTB2 gpa-13 Guanine nucleotide-binding protein alpha-13 subunit Caenorhabditis elegans SS
O76584 gpa-11 Guanine nucleotide-binding protein alpha-11 subunit Caenorhabditis elegans SS
P49084 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Glycine max (Soybean) (Glycine hispida) SS
P93163 GPA2 Guanine nucleotide-binding protein alpha-2 subunit Glycine max (Soybean) (Glycine hispida) SS
O80462 XLG1 Extra-large guanine nucleotide-binding protein 1 Arabidopsis thaliana (Mouse-ear cress) PR
P18064 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Arabidopsis thaliana (Mouse-ear cress) SS
P26981 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MGCIMSQEDE AAKRRSKKID RLLKEDGENS MRTIKLLLLG AGESGKSTIL KQMRIIHDVG
70 80 90 100 110 120
YTTEERKVFR GVVYGNIILS LNAIIHAMEQ LKISFTTLDH ESDARKLLMF STTGEEDELP
130 140 150 160 170 180
EELVVLMKSV WSDSGIQKAL ERSREYQLND SAGYYLSQLD RICAPNYIPT QDDILRTRIK
190 200 210 220 230 240
TTGIVETQFV YKDRLFLVFD VGGQRSERKK WIHCFEDVTA LIFCVALSEY DMVLVEDCQT
250 260 270 280 290 300
NRMRESLKLF DSICNNKWFV ETSIILFLNK KDLFEEKIVR SPLTHCFPEY TGANNYEEAS
310 320 330 340 350
AYIQQQFEDM NKRTTGEKNQ EIYTQFTCAT DTNNIRFVFD AVTDIIIRDN LRTCGLY