Q9N2L7

SS
Gene name
plk-2 (Y71F9B.7)
Protein name
Serine/threonine-protein kinase plk-2
Names
EC 2.7.11.21 , Polo-like kinase 2
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_Y71F9B.7
EC number
2.7.11.21: Protein-serine/threonine kinases
Protein Class

Descriptions

Polo-like kinase (Plk) acts at various stages in early and late mitosis and is involved in the regulation of centrosome maturation and spindle assembly. The C-terminal region of Plk1 regulates its kinase activity through intramolecular binding. The C-terminal region of Plk1, which contains the polo-box domain, occludes the catalytic kinase domain in the same protein and this autoinhibition is relieved by removal of the C-terminal region. In human PLK1 (Q07832), the polo-box also plays an autoinhibitory role.

Autoinhibitory domains (AIDs)

362-632 (Polo box domain) SS

Target domain

36-287 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

176-198 (Activation loop from InterPro)

Target domain

36-287 (Protein kinase domain)

Relief mechanism

Assay

176-198 (Activation loop from InterPro)

Target domain

36-287 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q9N2L7

Entry ID Method Resolution Chain Position Source
AF-Q9N2L7-F1 Predicted AlphaFoldDB

No variants for Q9N2L7

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9N2L7

No associated diseases with Q9N2L7

5 regional properties for Q9N2L7

Type Name Position InterPro Accession
domain Formin, FH3 domain 283 - 632 IPR010472
domain Formin, GTPase-binding domain 27 - 280 IPR010473
domain Diaphanous autoregulatory (DAD) domain 1049 - 1082 IPR014767
domain Rho GTPase-binding/formin homology 3 (GBD/FH3) domain 27 - 464 IPR014768
domain Formin, FH2 domain 627 - 1057 IPR015425

Functions

Description
EC Number 2.7.11.21 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
  • Cytoplasm
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Chromosome, centromere, kinetochore
  • Chromosome
  • During meiosis I, localizes to chromosome pairing centers formed at the nuclear envelope in early pachytene and then to the synaptonemal complex in mid and late pachytene (PubMed:22018921, PubMed:22018922)
  • Co-localizes at pairing centers with him-8, zim-3, zyg-12 and sun-1 and partially with plk-1 in early prophase (PubMed:22018921, PubMed:22018922)
  • Co-localizes with syp-1 in synapsed chromosomes at pachytene (PubMed:22018921)
  • Co-localizes with brc-1 in pachytene nuclei (PubMed:30383754)
  • Localizes asymmetrically in the 1-cell embryo which results in higher levels in the somatic cell AB than in the germline blastomere P1 (PubMed:18199581)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

3 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

4 GO annotations of biological process

Name Definition
meiotic cell cycle Progression through the phases of the meiotic cell cycle, in which canonically a cell replicates to produce four offspring with half the chromosomal content of the progenitor cell via two nuclear divisions.
mitotic spindle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
regulation of meiosis I Any process that modulates the rate, frequency, or extent of meiosis I, a cell cycle process comprising the steps by which a cell progresses through the first phase of meiosis, in which cells divide and homologous chromosomes are paired and segregated from each other, producing two daughter cells.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2TA25 PLK1 Serine/threonine-protein kinase PLK1 Bos taurus (Bovine) SS
O97143 SAK Serine/threonine-protein kinase PLK4 Drosophila melanogaster (Fruit fly) EV
P52304 polo Serine/threonine-protein kinase polo Drosophila melanogaster (Fruit fly) SS
P53350 PLK1 Serine/threonine-protein kinase PLK1 Homo sapiens (Human) EV
Q07832 Plk1 Serine/threonine-protein kinase PLK1 Mus musculus (Mouse) EV
Q62673 Plk1 Serine/threonine-protein kinase PLK1 Rattus norvegicus (Rat) SS
Q20845 plk-3 Serine/threonine-protein kinase plk-3 Caenorhabditis elegans PR
P34331 plk-1 Serine/threonine-protein kinase plk-1 Caenorhabditis elegans SS
P62205 plk1 Serine/threonine-protein kinase PLK1 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
10 20 30 40 50 60
MQRVQPSAAR VKSQKKEKAP PDVPDVILDG ERKTRYEKGK FLGKGGFAHC YELRNKSTGE
70 80 90 100 110 120
LFAGKVVPKA LLIKQYQRDK MAQEVQIHRN LQHRNVVKLY HFFEDKSNVY ITLELCPRRS
130 140 150 160 170 180
LMELHKRRKA VTEPEARYFT YQIVEGVLYL HNLKIVHRDL KLGNLFLNDE LQVKIGDFGL
190 200 210 220 230 240
ATTCDNDERK KTLCGTPNYI APEVLNKIGH SFEVDLWAIG CILYILLFGH PPFESKSLEE
250 260 270 280 290 300
TYSRIKNNNY VIPTSASAAA SQLIRVLLDP VPSRRPNARA VCRDHFFKSG FMPARLPVSC
310 320 330 340 350 360
LTMVPHLNDD EYAEENVSPS GTIDQRGPHQ AGRSGLSAIP AHLVSRNSER QQTHRMEAYR
370 380 390 400 410 420
QPTDCYLSNL LAQVNDLLAT PTADIDDAEA ALDSYQSPEA LPVFWISKWV DYSDKYGIGY
430 440 450 460 470 480
QLCDNSVGVL FNDNSRIMLD TAGTQLTYIE KTEKEHYFDM ESAIPSGLQK KMTLLKYFRS
490 500 510 520 530 540
YMNDHLLQAG QQVTRKVGDD LARLPTLRVW FRTKSAIVLH LSNGTVQINF FNDHIKMVLC
550 560 570 580 590 600
PLMQAVTFID ENKRMFTYKF SHLAENGCPE KFLHRIQYAK CMIQRLVEEH TKEETKHNAP
610 620 630
AANAVRLPST SSNVRLESAA DIQPAYPSSS RR