AiPD: Autoinhibited Protein Database

Gene name	UBC32 (At3g17000, K14A17.7)
Protein name	Ubiquitin-conjugating enzyme E2 32
Names	E2 ubiquitin-conjugating enzyme 32, Ubiquitin carrier protein 32
Species	Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway	ath:AT3G17000
EC number	2.3.2.23: Aminoacyltransferases
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

1-50 (Predicted disordered autoinhibitory region) PR

1-50 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q9LSP7-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Diseaes Association	Provenance
tmp_3_5797990_T_G	69	D>E	No	1000Genomes
ENSVATH05847670	139	R>K	No	1000Genomes
ENSVATH05847671	149	T>S	No	1000Genomes
tmp_3_5798486_C_A	156	P>T	No	1000Genomes
tmp_3_5798496_A_G	159	Q>R	No	1000Genomes
tmp_3_5799075_C_T	180	L>F	No	1000Genomes
ENSVATH05847683	181	P>L	No	1000Genomes
ENSVATH00328246	187	A>V	No	1000Genomes
ENSVATH05847685	200	P>A	No	1000Genomes
ENSVATH10732254	223	I>V	No	1000Genomes
ENSVATH05847686	236	S>N	No	1000Genomes
tmp_3_5799265_C_T	243	P>L	No	1000Genomes
ENSVATH00328247	244	L>I	No	1000Genomes
ENSVATH00328248	250	V>A	No	1000Genomes
ENSVATH05847687	255	V>A	No	1000Genomes
ENSVATH05847687	255	V>E	No	1000Genomes
tmp_3_5799320_G_A	261	M>I	No	1000Genomes
ENSVATH05847688	261	M>V	No	1000Genomes
ENSVATH00328249	262	A>T	No	1000Genomes
tmp_3_5799342_C_A	269	P>T	No	1000Genomes
ENSVATH13933787	270	V>I	No	1000Genomes
tmp_3_5799367_G_T	277	W>L	No	1000Genomes
ENSVATH05847691	302	T>A	No	1000Genomes
tmp_3_5799444_G_A	303	G>R	No	1000Genomes

Type	Name	Position	InterPro Accession
domain	Ubiquitin-conjugating enzyme E2	11 - 166	IPR000608

		Description
EC Number	2.3.2.23	Aminoacyltransferases
Subcellular Localization	Membrane ; Single-pass membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
endoplasmic reticulum	The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
integral component of membrane	The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
membrane	A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm	Cytoplasm situated near, or occurring around, the nucleus.

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ubiquitin conjugating enzyme activity	Isoenergetic transfer of ubiquitin from one protein to another via the reaction X-ubiquitin + Y -> Y-ubiquitin + X, where both the X-ubiquitin and Y-ubiquitin linkages are thioester bonds between the C-terminal glycine of ubiquitin and a sulfhydryl side group of a cysteine residue.
ubiquitin-protein transferase activity	Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.

Name	Definition
cellular response to water deprivation	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of water.
negative regulation of stomatal opening	Any process that stops, prevents or reduces the frequency, rate or extent of stomatal opening.
protein polyubiquitination	Addition of multiple ubiquitin groups to a protein, forming a ubiquitin chain.
ubiquitin-dependent protein catabolic process	The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q9Y385	UBE2J1	Ubiquitin-conjugating enzyme E2 J1	Homo sapiens (Human)	PR
Q9JJZ4	Ube2j1	Ubiquitin-conjugating enzyme E2 J1	Mus musculus (Mouse)	PR
Q9C8X7	UBC31	Probable ubiquitin-conjugating enzyme E2 31	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9SLE4	UBC29	Ubiquitin-conjugating enzyme E2 29	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9FF66	UBC22	Ubiquitin-conjugating enzyme E2 22	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9LJD7	COP10	Constitutive photomorphogenesis protein 10	Arabidopsis thaliana (Mouse-ear cress)	PR