Q9JLM8
SS
Gene name |
Dclk1 (Dcamkl1, Dclk) |
Protein name |
Serine/threonine-protein kinase DCLK1 |
Names |
EC 2.7.11.1 , Doublecortin-like and CAM kinase-like 1 , Doublecortin-like kinase 1 |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:13175 |
EC number |
2.7.11.1: Protein-serine/threonine kinases |
Protein Class |
|
Descriptions
Doublecortin-like kinase 1 (DCLK1) is upregulated in many tumors and is a marker for tumor stem cells. DCLK1 contains the N-terminal tandem doublecortin domains and the C-terminal Ser/Thr kinase domain. The C-terminal kinase is autoinhibited by a C-terminal autoinhibitory domain), which consists of an arginine-rich region to function as a pseudosubstrate for DCLK1 by binding to the cleft between the two lobes of the kinase domain, blocking the ATP and peptide substrate-binding site and inhibiting DCLK1 kinase activity in an ATP-competitive manner. HPCAL1, a member of visinin-like-proteins (VILIPs), belongs to the neuronal calcium sensor (NCS) family, which are calcium-binding proteins involved in the regulation of neuronal Ca2+ signaling. HPCAL1 directly binds the AID of DCLK1 in a Ca2+-dependent manner to activate DCLK1 kinase activity.
Autoinhibitory domains (AIDs)
Target domain |
406-663 (Protein kinase domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
548-568 (Activation loop from InterPro)
Target domain |
406-663 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
548-568 (Activation loop from InterPro)
Target domain |
406-663 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
Autoinhibited structure
Activated structure
1 structures for Q9JLM8
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9JLM8-F1 | Predicted | AlphaFoldDB |
35 variants for Q9JLM8
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3388631933 | 31 | P>H | No | EVA | |
| rs3388625232 | 35 | H>Y | No | EVA | |
| rs30572221 | 48 | Q>H | No | EVA | |
| rs3388627755 | 50 | L>V | No | EVA | |
| rs3388627735 | 72 | I>F | No | EVA | |
| rs3388631946 | 77 | S>F | No | EVA | |
| rs3388629978 | 113 | G>V | No | EVA | |
| rs3388631110 | 124 | V>M | No | EVA | |
| rs3388627769 | 132 | G>C | No | EVA | |
| rs3388625638 | 145 | N>D | No | EVA | |
| rs3388612637 | 178 | E>K | No | EVA | |
| rs3388623781 | 200 | A>V | No | EVA | |
| rs3388633699 | 286 | K>Q | No | EVA | |
| rs3388627272 | 296 | T>I | No | EVA | |
| rs221862311 | 359 | T>I | No | EVA | |
| rs3388627216 | 364 | S>L | No | EVA | |
| rs3410929108 | 416 | N>K | No | EVA | |
| rs3388631584 | 465 | V>L | No | EVA | |
| rs3388619145 | 495 | S>P | No | EVA | |
| rs3388633056 | 522 | N>K | No | EVA | |
| rs3388631978 | 522 | N>Y | No | EVA | |
| rs3388627757 | 530 | P>S | No | EVA | |
| rs3388629198 | 543 | K>M | No | EVA | |
| rs3388629987 | 544 | S>T | No | EVA | |
| rs3392869044 | 545 | L>P | No | EVA | |
| rs3393068043 | 546 | K>N | No | EVA | |
| rs3392879265 | 548 | G>S | No | EVA | |
| rs3388629937 | 581 | G>R | No | EVA | |
| rs3388623827 | 586 | I>L | No | EVA | |
| rs3393056721 | 587 | W>* | No | EVA | |
| rs3388629209 | 608 | D>Y | No | EVA | |
| rs3388629164 | 650 | Q>H | No | EVA | |
| rs30593547 | 655 | V>M | No | EVA | |
| rs3388627697 | 700 | V>E | No | EVA | |
| rs3388619112 | 713 | F>L | No | EVA |
No associated diseases with Q9JLM8
5 regional properties for Q9JLM8
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 406 - 663 | IPR000719 |
| domain | Doublecortin domain | 52 - 143 | IPR003533-1 |
| domain | Doublecortin domain | 181 - 269 | IPR003533-2 |
| active_site | Serine/threonine-protein kinase, active site | 523 - 535 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 412 - 439 | IPR017441 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.1 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| axon | The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter. |
| growth cone | The migrating motile tip of a growing neuron projection, where actin accumulates, and the actin cytoskeleton is the most dynamic. |
| postsynaptic density | An electron dense network of proteins within and adjacent to the postsynaptic membrane of an asymmetric, neuron-neuron synapse. Its major components include neurotransmitter receptors and the proteins that spatially and functionally organize them such as anchoring and scaffolding molecules, signaling enzymes and cytoskeletal components. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| protein kinase activity | Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction |
| protein serine kinase activity | Catalysis of the reactions |
| protein serine/threonine kinase activity | Catalysis of the reactions |
13 GO annotations of biological process
| Name | Definition |
|---|---|
| axon extension | Long distance growth of a single axon process involved in cellular development. |
| axonogenesis | De novo generation of a long process of a neuron, including the terminal branched region. Refers to the morphogenesis or creation of shape or form of the developing axon, which carries efferent (outgoing) action potentials from the cell body towards target cells. |
| brain development | The process whose specific outcome is the progression of the brain over time, from its formation to the mature structure. Brain development begins with patterning events in the neural tube and ends with the mature structure that is the center of thought and emotion. The brain is responsible for the coordination and control of bodily activities and the interpretation of information from the senses (sight, hearing, smell, etc.). |
| central nervous system projection neuron axonogenesis | Generation of a long process of a CNS neuron, that carries efferent (outgoing) action potentials from the cell body towards target cells in a different central nervous system region. |
| dendrite morphogenesis | The process in which the anatomical structures of a dendrite are generated and organized. |
| forebrain development | The process whose specific outcome is the progression of the forebrain over time, from its formation to the mature structure. The forebrain is the anterior of the three primary divisions of the developing chordate brain or the corresponding part of the adult brain (in vertebrates, includes especially the cerebral hemispheres, the thalamus, and the hypothalamus and especially in higher vertebrates is the main control center for sensory and associative information processing, visceral functions, and voluntary motor functions). |
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| negative regulation of protein localization to nucleus | Any process that stops, prevents or reduces the frequency, rate or extent of protein localization to nucleus. |
| neuron migration | The characteristic movement of an immature neuron from germinal zones to specific positions where they will reside as they mature. |
| neuron projection morphogenesis | The process in which the anatomical structures of a neuron projection are generated and organized. A neuron projection is any process extending from a neural cell, such as axons or dendrites. |
| phosphorylation | The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. |
| protein localization to nucleus | A process in which a protein transports or maintains the localization of another protein to the nucleus. |
| response to virus | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a virus. |
103 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| A4IFM7 | MYLK2 | Myosin light chain kinase 2, skeletal/cardiac muscle | Bos taurus (Bovine) | SS |
| Q0V7M1 | PSKH1 | Serine/threonine-protein kinase H1 | Bos taurus (Bovine) | SS |
| Q0VD22 | STK33 | Serine/threonine-protein kinase 33 | Bos taurus (Bovine) | PR |
| Q9VUI3 | DCX-EMAP | Echinoderm microtubule-associated protein-like CG42247 | Drosophila melanogaster (Fruit fly) | PR |
| Q96NX5 | CAMK1G | Calcium/calmodulin-dependent protein kinase type 1G | Homo sapiens (Human) | SS |
| Q14012 | CAMK1 | Calcium/calmodulin-dependent protein kinase type 1 | Homo sapiens (Human) | EV |
| Q9UIK4 | DAPK2 | Death-associated protein kinase 2 | Homo sapiens (Human) | EV |
| O94768 | STK17B | Serine/threonine-protein kinase 17B | Homo sapiens (Human) | PR |
| Q86YV6 | MYLK4 | Myosin light chain kinase family member 4 | Homo sapiens (Human) | SS |
| Q16566 | CAMK4 | Calcium/calmodulin-dependent protein kinase type IV | Homo sapiens (Human) | SS |
| P11801 | PSKH1 | Serine/threonine-protein kinase H1 | Homo sapiens (Human) | SS |
| Q9UEE5 | STK17A | Serine/threonine-protein kinase 17A | Homo sapiens (Human) | SS |
| O75962 | TRIO | Triple functional domain protein | Homo sapiens (Human) | EV |
| Q9H1R3 | MYLK2 | Myosin light chain kinase 2, skeletal/cardiac muscle | Homo sapiens (Human) | EV |
| Q32MK0 | MYLK3 | Myosin light chain kinase 3 | Homo sapiens (Human) | SS |
| Q8WWF8 | CAPSL | Calcyphosin-like protein | Homo sapiens (Human) | PR |
| P53355 | DAPK1 | Death-associated protein kinase 1 | Homo sapiens (Human) | EV |
| Q6P2M8 | PNCK | Calcium/calmodulin-dependent protein kinase type 1B | Homo sapiens (Human) | SS |
| Q9BYT3 | STK33 | Serine/threonine-protein kinase 33 | Homo sapiens (Human) | PR |
| Q8NCB2 | CAMKV | CaM kinase-like vesicle-associated protein | Homo sapiens (Human) | SS |
| O43293 | DAPK3 | Death-associated protein kinase 3 | Homo sapiens (Human) | PR |
| Q8IU85 | CAMK1D | Calcium/calmodulin-dependent protein kinase type 1D | Homo sapiens (Human) | SS |
| Q9UHG0 | DCDC2 | Doublecortin domain-containing protein 2 | Homo sapiens (Human) | PR |
| Q8N568 | DCLK2 | Serine/threonine-protein kinase DCLK2 | Homo sapiens (Human) | PR |
| O15075 | DCLK1 | Serine/threonine-protein kinase DCLK1 | Homo sapiens (Human) | EV |
| Q9QYK9 | Pnck | Calcium/calmodulin-dependent protein kinase type 1B | Mus musculus (Mouse) | PR |
| Q91VB2 | Camk1g | Calcium/calmodulin-dependent protein kinase type 1G | Mus musculus (Mouse) | SS |
| Q8BG48 | Stk17b | Serine/threonine-protein kinase 17B | Mus musculus (Mouse) | PR |
| Q8VCR8 | Mylk2 | Myosin light chain kinase 2, skeletal/cardiac muscle | Mus musculus (Mouse) | SS |
| Q91YS8 | Camk1 | Calcium/calmodulin-dependent protein kinase type 1 | Mus musculus (Mouse) | SS |
| Q3UIZ8 | Mylk3 | Myosin light chain kinase 3 | Mus musculus (Mouse) | SS |
| Q8VDF3 | Dapk2 | Death-associated protein kinase 2 | Mus musculus (Mouse) | EV |
| Q6P8Y1 | Capsl | Calcyphosin-like protein | Mus musculus (Mouse) | PR |
| Q8BW96 | Camk1d | Calcium/calmodulin-dependent protein kinase type 1D | Mus musculus (Mouse) | SS |
| Q3UHL1 | Camkv | CaM kinase-like vesicle-associated protein | Mus musculus (Mouse) | SS |
| P08414 | Camk4 | Calcium/calmodulin-dependent protein kinase type IV | Mus musculus (Mouse) | SS |
| Q91YA2 | Pskh1 | Serine/threonine-protein kinase H1 | Mus musculus (Mouse) | SS |
| Q80YE7 | Dapk1 | Death-associated protein kinase 1 | Mus musculus (Mouse) | SS |
| Q62407 | Speg | Striated muscle-specific serine/threonine-protein kinase | Mus musculus (Mouse) | SS |
| Q0KL02 | Trio | Triple functional domain protein | Mus musculus (Mouse) | SS |
| Q924X7 | Stk33 | Serine/threonine-protein kinase 33 | Mus musculus (Mouse) | PR |
| O54784 | Dapk3 | Death-associated protein kinase 3 | Mus musculus (Mouse) | PR |
| Q5DU00 | Dcdc2 | Doublecortin domain-containing protein 2 | Mus musculus (Mouse) | PR |
| Q6PGN3 | Dclk2 | Serine/threonine-protein kinase DCLK2 | Mus musculus (Mouse) | PR |
| Q7TNJ7 | Camk1g | Calcium/calmodulin-dependent protein kinase type 1G | Rattus norvegicus (Rat) | SS |
| P20689 | Mylk2 | Myosin light chain kinase 2, skeletal/cardiac muscle | Rattus norvegicus (Rat) | SS |
| F1M0Z1 | Trio | Triple functional domain protein | Rattus norvegicus (Rat) | SS |
| Q91XS8 | Stk17b | Serine/threonine-protein kinase 17B | Rattus norvegicus (Rat) | PR |
| E9PT87 | Mylk3 | Myosin light chain kinase 3 | Rattus norvegicus (Rat) | SS |
| O70150 | Pnck | Calcium/calmodulin-dependent protein kinase type 1B | Rattus norvegicus (Rat) | PR |
| Q63092 | Camkv | CaM kinase-like vesicle-associated protein | Rattus norvegicus (Rat) | SS |
| Q63638 | Speg | Striated muscle-specific serine/threonine-protein kinase | Rattus norvegicus (Rat) | SS |
| O88764 | Dapk3 | Death-associated protein kinase 3 | Rattus norvegicus (Rat) | PR |
| P13234 | Camk4 | Calcium/calmodulin-dependent protein kinase type IV | Rattus norvegicus (Rat) | SS |
| Q63450 | Camk1 | Calcium/calmodulin-dependent protein kinase type 1 | Rattus norvegicus (Rat) | EV |
| P97924 | Kalrn | Kalirin | Rattus norvegicus (Rat) | SS |
| Q5MPA9 | Dclk2 | Serine/threonine-protein kinase DCLK2 | Rattus norvegicus (Rat) | PR |
| O08875 | Dclk1 | Serine/threonine-protein kinase DCLK1 | Rattus norvegicus (Rat) | PR |
| Q5VQQ5 | CPK2 | Calcium-dependent protein kinase 2 | Oryza sativa subsp. japonica (Rice) | SS |
| Q8LPZ7 | CPK3 | Calcium-dependent protein kinase 3 | Oryza sativa subsp. japonica (Rice) | SS |
| Q6K968 | CPK6 | Calcium-dependent protein kinase 6 | Oryza sativa subsp. japonica (Rice) | SS |
| P53684 | CPK7 | Calcium-dependent protein kinase 7 | Oryza sativa subsp japonica (Rice) | PR |
| Q75GE8 | CPK8 | Calcium-dependent protein kinase 8 | Oryza sativa subsp. japonica (Rice) | SS |
| Q6I5I8 | CPK16 | Calcium-dependent protein kinase 16 | Oryza sativa subsp. japonica (Rice) | SS |
| Q6AVM3 | CCAMK | Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase | Oryza sativa subsp japonica (Rice) | PR |
| Q84SL0 | CPK20 | Calcium-dependent protein kinase 20 | Oryza sativa subsp. japonica (Rice) | SS |
| P53682 | CPK23 | Calcium-dependent protein kinase 23 | Oryza sativa subsp japonica (Rice) | PR |
| Q9TXJ0 | cmk-1 | Calcium/calmodulin-dependent protein kinase type 1 | Caenorhabditis elegans | PR |
| O44997 | dapk-1 | Death-associated protein kinase dapk-1 | Caenorhabditis elegans | SS |
| Q95QC4 | zyg-8 | Serine/threonine-protein kinase zyg-8 | Caenorhabditis elegans | PR |
| P28583 | Calcium-dependent protein kinase SK5 | Glycine max (Soybean) (Glycine hispida) | PR | |
| Q9M9V8 | CPK10 | Calcium-dependent protein kinase 10 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q39016 | CPK11 | Calcium-dependent protein kinase 11 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FX86 | CRK8 | CDPK-related kinase 8 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SSF8 | CPK30 | Calcium-dependent protein kinase 30 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38872 | CPK6 | Calcium-dependent protein kinase 6 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q7XJR9 | CPK16 | Calcium-dependent protein kinase 16 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SIQ7 | CPK24 | Calcium-dependent protein kinase 24 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SJ61 | CPK25 | Calcium-dependent protein kinase 25 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9ZV15 | CPK20 | Calcium-dependent protein kinase 20 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| P93759 | GK-1 | Calcium-dependent protein kinase 14 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q8S8S2 | LPEAT2 | Lysophospholipid acyltransferase LPEAT2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38870 | CPK2 | Calcium-dependent protein kinase 2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9LJL9 | CRK2 | CDPK-related kinase 2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SG12 | CRK6 | CDPK-related kinase 6 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SCS2 | CRK5 | CDPK-related kinase 5 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q8W4I7 | CPK13 | Calcium-dependent protein kinase 13 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q6NLQ6 | CPK32 | Calcium-dependent protein kinase 32 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9ZSA4 | CPK27 | Calcium-dependent protein kinase 27 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38869 | CPK4 | Calcium-dependent protein kinase 4 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q42479 | CPK3 | Calcium-dependent protein kinase 3 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q1PE17 | CPK18 | Calcium-dependent protein kinase 18 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q06850 | CPK1 | Calcium-dependent protein kinase 1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9FMP5 | CPK17 | Calcium-dependent protein kinase 17 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38873 | CPK7 | Calcium-dependent protein kinase 7 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q3E9C0 | CPK34 | Calcium-dependent protein kinase 34 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q42438 | CPK8 | Calcium-dependent protein kinase 8 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q42396 | CPK12 | Calcium-dependent protein kinase 12 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FKW4 | CPK28 | Calcium-dependent protein kinase 28 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9SUA3 | D6PKL1 | Serine/threonine-protein kinase D6PKL1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q1LUA6 | trio | Triple functional domain protein | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| A8C984 | mylk3 | Myosin light chain kinase 3 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| Q7SY49 | camkv | CaM kinase-like vesicle-associated protein | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSFGRDMELE | HFDERDKAQR | YSRGSRVNGL | PSPTHSAHCS | FYRTRTLQTL | SSEKKAKKVR |
| 70 | 80 | 90 | 100 | 110 | 120 |
| FYRNGDRYFK | GIVYAISPDR | FRSFEALLAD | LTRTLSDNVN | LPQGVRTIYT | IDGLKKISSL |
| 130 | 140 | 150 | 160 | 170 | 180 |
| DQLVEGESYV | CGSIEPFKKL | EYTKNVNPNW | SVNVKTTSAS | RAVSSLATAK | GGPSEVRENK |
| 190 | 200 | 210 | 220 | 230 | 240 |
| DFIRPKLVTI | IRSGVKPRKA | VRILLNKKTA | HSFEQVLTDI | TDAIKLDSGV | VKRLYTLDGK |
| 250 | 260 | 270 | 280 | 290 | 300 |
| QVMCLQDFFG | DDDIFIACGP | EKFRYQDDFL | LDESECRVVK | STSYTKIASA | SRRGTTKSPG |
| 310 | 320 | 330 | 340 | 350 | 360 |
| PSRRSKSPAS | TSSVNGTPGS | QLSTPRSGKS | PSPSPTSPGS | LRKQRISQHG | GSSTSLSSTK |
| 370 | 380 | 390 | 400 | 410 | 420 |
| VCSSMDENDG | PGEGDELGRR | HSLQRGWRRE | ESEEGFQIPA | TITERYKVGR | TIGDGNFAVV |
| 430 | 440 | 450 | 460 | 470 | 480 |
| KECIERSTAR | EYALKIIKKS | KCRGKEHMIQ | NEVSILRRVK | HPNIVLLIEE | MDVPTELYLV |
| 490 | 500 | 510 | 520 | 530 | 540 |
| MELVKGGDLF | DAITSTSKYT | ERDASGMLYN | LASAIKYLHS | LNIVHRDIKP | ENLLVYEHQD |
| 550 | 560 | 570 | 580 | 590 | 600 |
| GSKSLKLGDF | GLATIVDGPL | YTVCGTPTYV | APEIIAETGY | GLKVDIWAAG | VITYILLCGF |
| 610 | 620 | 630 | 640 | 650 | 660 |
| PPFRGSGDDQ | EVLFDQILMG | QVDFPSPYWD | NVSDSAKELI | NMMLLVNVDQ | RFSAVQVLEH |
| 670 | 680 | 690 | 700 | 710 | 720 |
| PWVNDDGLPE | NEHQLSVAGK | IKKHFNTGPK | PSSTAAGVSV | IATTALDKER | QVFRRRRNQD |
| 730 | 740 | 750 | |||
| VRSRYKAQPA | PPELNSESED | YSPSSSETVR | SPNSPF |