Q9JKF1

SS
Gene name
Iqgap1
Protein name
Ras GTPase-activating-like protein IQGAP1
Names
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:29875
EC number
Protein Class

Descriptions

Ras GTPase-activating-like protein IQGAP1 plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. IQGAP1 binds directly nucleotide-depleted Cdc42 (Cdc42-ND) and may serve as a negative effector or sequester nucleotide-free Cdc42 to prevent signaling. The regions C1 (956-1274) and C2 (1276-1657) of IQGAP1 can either interact with nucleotide-free Cdc42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free Cdc42 and promotes binding of GTP-bound Cdc42. The phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free Cdc42 on both C1 and C2.

Autoinhibitory domains (AIDs)

1276-1657 (C2 fragments) SS

Target domain

956-1274 (C1 fragments)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9JKF1

Entry ID Method Resolution Chain Position Source
AF-Q9JKF1-F1 Predicted AlphaFoldDB

7 variants for Q9JKF1

Variant ID(s) Position Change Description Diseaes Association Provenance
rs232712612 92 L>V No Ensembl
rs216296629 322 V>G No Ensembl
rs33220621 373 A>V No Ensembl
rs13471640 1006 I>M No Ensembl
rs233736618 1268 V>A No Ensembl
rs13471636 1268 V>L No Ensembl
rs244359156 1551 M>I No Ensembl

No associated diseases with Q9JKF1

9 regional properties for Q9JKF1

Type Name Position InterPro Accession
binding_site IQ motif, EF-hand binding site 744 - 774 IPR000048-1
binding_site IQ motif, EF-hand binding site 774 - 804 IPR000048-2
binding_site IQ motif, EF-hand binding site 804 - 834 IPR000048-3
binding_site IQ motif, EF-hand binding site 834 - 864 IPR000048-4
domain RasGAP protein, C-terminal 1452 - 1580 IPR000593
domain WW domain 685 - 712 IPR001202
domain Calponin homology domain 44 - 159 IPR001715
domain Ras GTPase-activating domain 992 - 1345 IPR001936
conserved_site Ras GTPase-activating protein, conserved site 1189 - 1203 IPR023152

Functions

Description
EC Number
Subcellular Localization
  • Cell membrane
  • Nucleus
  • Cytoplasm
  • Apical cell membrane
  • Basolateral cell membrane
  • Subcellular distribution is regulated by the cell cycle, nuclear levels increase at G1/S phase (PubMed:20883816)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

20 GO annotations of cellular component

Name Definition
axon The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter.
cell leading edge The area of a motile cell closest to the direction of movement.
cell-cell junction A cell junction that forms a connection between two or more cells of an organism; excludes direct cytoplasmic intercellular bridges, such as ring canals in insects.
cortical actin cytoskeleton The portion of the actin cytoskeleton, comprising filamentous actin and associated proteins, that lies just beneath the plasma membrane.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic ribonucleoprotein granule A ribonucleoprotein granule located in the cytoplasm.
extrinsic component of cytoplasmic side of plasma membrane The component of a plasma membrane consisting of gene products and protein complexes that are loosely bound to its cytoplasmic surface, but not integrated into the hydrophobic region.
focal adhesion A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
growth cone The migrating motile tip of a growing neuron projection, where actin accumulates, and the actin cytoskeleton is the most dynamic.
lateral plasma membrane The portion of the plasma membrane at the lateral side of the cell. In epithelial cells, lateral plasma membranes are on the sides of cells which lie at the interface of adjacent cells.
membrane raft Any of the small (10-200 nm), heterogeneous, highly dynamic, sterol- and sphingolipid-enriched membrane domains that compartmentalize cellular processes. Small rafts can sometimes be stabilized to form larger platforms through protein-protein and protein-lipid interactions.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
neuron projection A prolongation or process extending from a nerve cell, e.g. an axon or dendrite.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
ribonucleoprotein complex A macromolecular complex that contains both RNA and protein molecules.
ruffle Projection at the leading edge of a crawling cell; the protrusions are supported by a microfilament meshwork.
slit diaphragm A specialized cell-cell junction found between the interdigitating foot processes of the glomerular epithelium (the podocytes) in the vertebrate kidney, which is adapted for facilitating glomerular filtration.

15 GO annotations of molecular function

Name Definition
actin filament binding Binding to an actin filament, also known as F-actin, a helical filamentous polymer of globular G-actin subunits.
calcium ion binding Binding to a calcium ion (Ca2+).
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
GTPase activator activity Binds to and increases the activity of a GTPase, an enzyme that catalyzes the hydrolysis of GTP.
MAP-kinase scaffold activity The binding activity of a molecule that functions as a physical support for the assembly of a multiprotein mitogen-activated protein kinase (MAPK) complex. Binds multiple kinases of the MAPKKK cascade, and also upstream signaling proteins, permitting those molecules to function in a coordinated way. Bringing together multiple enzymes and their substrates enables the signal to be transduced quickly and efficiently.
mitogen-activated protein kinase binding Binding to a mitogen-activated protein kinase.
molecular adaptor activity The binding activity of a molecule that brings together two or more molecules through a selective, non-covalent, often stoichiometric interaction, permitting those molecules to function in a coordinated way.
phosphatidylinositol-3,4,5-trisphosphate binding Binding to phosphatidylinositol-3,4,5-trisphosphate, a derivative of phosphatidylinositol in which the inositol ring is phosphorylated at the 3', 4' and 5' positions.
protein domain specific binding Binding to a specific domain of a protein.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein phosphatase binding Binding to a protein phosphatase.
protein serine/threonine kinase activator activity Binds to and increases the activity of a protein serine/threonine kinase.
protein-containing complex binding Binding to a macromolecular complex.
S100 protein binding Binding to a S100 protein. S100 is a small calcium and zinc binding protein produced in astrocytes that is implicated in Alzheimer's disease, Down Syndrome and ALS.
small GTPase binding Binding to a small monomeric GTPase.

25 GO annotations of biological process

Name Definition
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues. Cell migration is a central process in the development and maintenance of multicellular organisms.
cellular response to calcium ion Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a calcium ion stimulus.
cellular response to epidermal growth factor stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an epidermal growth factor stimulus.
cellular response to fibroblast growth factor stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an fibroblast growth factor stimulus.
cellular response to platelet-derived growth factor stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a platelet-derived growth factor stimulus.
epidermal growth factor receptor signaling pathway The series of molecular signals initiated by binding of a ligand to the tyrosine kinase receptor EGFR (ERBB1) on the surface of a cell. The pathway ends with regulation of a downstream cellular process, e.g. transcription.
fibroblast growth factor receptor signaling pathway The series of molecular signals generated as a consequence of a fibroblast growth factor receptor binding to one of its physiological ligands.
fibroblast migration Cell migration that is accomplished by extension and retraction of a fibroblast pseudopodium. A fibroblast is a connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules.
glomerular visceral epithelial cell development The process whose specific outcome is the progression of a glomerular visceral epithelial cell over time, from its formation to the mature structure. A glomerular visceral epithelial cell is a specialized epithelial cell that contains 'feet' that interdigitate with the 'feet' of other glomerular epithelial cells.
negative regulation of dephosphorylation Any process the stops, prevents, or reduces the frequency, rate or extent of removal of phosphate groups from a molecule.
neuron projection extension Long distance growth of a single neuron projection involved in cellular development. A neuron projection is a prolongation or process extending from a nerve cell, e.g. an axon or dendrite.
platelet-derived growth factor receptor signaling pathway The series of molecular signals initiated by a ligand binding to a platelet-derived growth factor receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
positive regulation of dendrite development Any process that activates or increases the frequency, rate or extent of dendrite development.
positive regulation of focal adhesion assembly Any process that activates or increases the frequency, rate or extent of focal adhesion assembly, the establishment and maturation of focal adhesions.
positive regulation of MAP kinase activity Any process that activates or increases the frequency, rate or extent of MAP kinase activity.
positive regulation of MAPK cascade Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the MAPK cascade.
positive regulation of peptidyl-tyrosine autophosphorylation Any process that activates or increases the frequency, rate or extent of peptidyl-tyrosine autophosphorylation.
positive regulation of protein kinase activity Any process that activates or increases the frequency, rate or extent of protein kinase activity.
positive regulation of protein localization Any process that activates or increases the frequency, rate or extent of a protein localization.
positive regulation of vascular associated smooth muscle cell migration Any process that activates or increases the frequency, rate or extent of vascular associated smooth muscle cell migration.
regulation of actin cytoskeleton organization Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins.
regulation of cytokine production Any process that modulates the frequency, rate, or extent of production of a cytokine.
regulation of GTPase activity Any process that modulates the rate of GTP hydrolysis by a GTPase.
regulation of mitotic cell cycle Any process that modulates the rate or extent of progress through the mitotic cell cycle.
response to angiotensin Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an angiotensin stimulus. Angiotensin is any of three physiologically active peptides (angiotensin II, III, or IV) processed from angiotensinogen.

4 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q13576 IQGAP2 Ras GTPase-activating-like protein IQGAP2 Homo sapiens (Human) SS
P46940 IQGAP1 Ras GTPase-activating-like protein IQGAP1 Homo sapiens (Human) EV
Q86VI3 IQGAP3 Ras GTPase-activating-like protein IQGAP3 Homo sapiens (Human) SS
Q3UQ44 Iqgap2 Ras GTPase-activating-like protein IQGAP2 Mus musculus (Mouse) SS
10 20 30 40 50 60
MSAAEEVDGL GVVRPHYGSV LDNERLTAEE MDERRRQNVA YEYLCHLEEA KRWMEACLGE
70 80 90 100 110 120
DLPPTTELEE GLRNGVYLAK LGNFFSPKVV SLKKIYDREQ TRYKATGLHF RHTDNVIQWL
130 140 150 160 170 180
NAMDEIGLPK IFYPETTDIY DRKNMPRCIY CIHALSLYLF KLGLAPQIQD LYGKVDFTEE
190 200 210 220 230 240
EINNMKIELE KYGIQMPAFS KIGGILANEL SVDEAALHAA VIAINEAIDR RVAADTFTAL
250 260 270 280 290 300
KNPNAMLVNL EEGLAPTYQD VLYQAKQDKM TNAKNRTENS DRERDVYEEL LTQAEIQGNV
310 320 330 340 350 360
NKVNTSSALA NISLALEQGC AVTLLKALQS LALGLRGLQT QNSDWYMKQL QSDLQQKRQS
370 380 390 400 410 420
GQTDPLQKEE VQAGVDAANS AAQQYQRRLA AVAAINAAIQ KGIAEKTVLE LMNPEAQLPQ
430 440 450 460 470 480
VYPFAADLYQ KELATLQQQS PEHSLTHPEL TVAVEMLSSV ALINRALESG DMTTVWKQLS
490 500 510 520 530 540
SSVTGLTNIE EENCQRYLDE LMKLKAQAHA ENNAFITWND IQACVDHVNL VVHEEHERIL
550 560 570 580 590 600
AIGLINEALD EGDAQKTLQA LQIPAAKLEG VLAEVAQHYQ DTLIRAKREK AQETQDESAV
610 620 630 640 650 660
LWLDEIQGGI WQSNKDTQEA QRFALGISAI NEAVDSGDVG RTLSALRSPD VGLYGVIPEC
670 680 690 700 710 720
GETYQSDLAE AKKKRLAAGD NNSKWVKHWV KGGYHYYHNL ETQAGGWAEP PDFVQNSVQL
730 740 750 760 770 780
SREEIQSSIS GVTAAYNREQ LWLANEGLIT KLQACCRGYL VRQEFRSRMN FLKKQIPAIT
790 800 810 820 830 840
CIQSQWRGYK QKKAYQDRLA YLHSHKDEVV KIQSLARMHQ ARKRYRDRLQ YFRDHINDII
850 860 870 880 890 900
KIQAFIRANK ARDDYKTLIN AEDPPMIVVR KFVHLLDQSD QDFQEELDLM KMREEVITLI
910 920 930 940 950 960
RSNQQLENDL NLMDIKIGLL VKNKITLQDV VSHSKKLTKK NKEQLSDMMM INKQKGGLKA
970 980 990 1000 1010 1020
LSKEKREKLE AYQHLFYLLQ TNPTYLAKLI FQMPQNKSTK FMDSVIFTLY NYASNQREEY
1030 1040 1050 1060 1070 1080
LLLRLFQTAL QEEIKSKVDQ IQEIVTGNPT VIKMVVSFNR GARGQNALRQ ILAPVVKEIM
1090 1100 1110 1120 1130 1140
DDKSLNIKTD PVDIYKSWVN QMESQTGEAS KLPYDVTPEQ ALSHEEVKTR LDNSIRNMRA
1150 1160 1170 1180 1190 1200
VTDKFLSAIV SSVDKIPYGM RFIAKVLKDS LHEKFPDAGE DELLKIIGNL LYYRYMNPAI
1210 1220 1230 1240 1250 1260
VAPDAFDIID LSAGGQLTTD QRRNLGSIAK MLQHAASNKM FLGDNAHLSI INEYLSQSYQ
1270 1280 1290 1300 1310 1320
KFRRFFQVAC DVPELQDKFN VDEYSDLVTL TKPVIYISIG EIINTHTLLL DHQDAIAPEH
1330 1340 1350 1360 1370 1380
NDPIHELLDD LGEVPTIESL IGESCGNSND PNKEALAKTE VSLTLTNKFD VPGDENAEMD
1390 1400 1410 1420 1430 1440
ARTILLNTKR LIVDVIRFQP GETLTEILET PATNEQEAEH QRAMQRRAIR DAKTPDKMKK
1450 1460 1470 1480 1490 1500
SKPMKEDNNL SLQEKKEKIQ TGLKKLTELG TVDPKNRYQE LINDIAKDIR NQRRYRQRRK
1510 1520 1530 1540 1550 1560
AELVKLQQTY SALNSKATFY GEQVDYYKSY IKTCLDNLAS KGKVSKKPRE MKGKKSKKIS
1570 1580 1590 1600 1610 1620
LKYTAARLHE KGVLLEIEDL QANQFKNVIF EIGPTEEVGD FEVKAKFMGV QMETFMLHYQ
1630 1640 1650
DLLQLQYEGV AVMKLFDRAK VNVNLLIFLL NKKFYGK