Q9JIH7

EV
Gene name
Wnk1
Protein name
Serine/threonine-protein kinase WNK1
Names
Protein kinase lysine-deficient 1, Protein kinase with no lysine 1
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:116477
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class
SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED (PTHR13902)

Descriptions

WNK family protein kinases are large enzymes that contain the catalytic lysine in a unique position compared with all other protein kinases. The activity of the Wnk1 kinase domain is controlled by its autoinhibitory domain, which is relieved through specific mutations of key residues in the domain. Activation of Wnk1 requires autophosphorylaiton of Ser382 within its activation loop, and phosphorylation of Ser378 enhances its kinase activity.

Autoinhibitory domains (AIDs)

514-537 (Autoinhibitory region) EV

Target domain

221-479 (Protein kinase domain)

Relief mechanism

PTM

Assay

Deletion assay, Mutagenesis experiment

Accessory elements

367-388 (Activation loop from InterPro)

Target domain

221-479 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

8 structures for Q9JIH7

Entry ID Method Resolution Chain Position Source
2LRU NMR - A 480-572 PDB
4Q2A X-ray 350 A A 194-480 PDB
5DRB X-ray 165 A A 194-483 PDB
5W7T X-ray 201 A A/B 210-482 PDB
6CN9 X-ray 180 A A/B 194-483 PDB
6OL2 X-ray 210 A A 194-483 PDB
7UOS X-ray 290 A A 194-483 PDB
AF-Q9JIH7-F1 Predicted AlphaFoldDB

No variants for Q9JIH7

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9JIH7

No associated diseases with Q9JIH7

12 regional properties for Q9JIH7

Type Name Position InterPro Accession
domain Protein kinase domain 619 - 882 IPR000719
domain Ephrin receptor ligand binding domain 1 - 182 IPR001090
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 619 - 878 IPR001245
conserved_site Tyrosine-protein kinase, receptor class V, conserved site 221 - 241 IPR001426
domain Sterile alpha motif domain 908 - 975 IPR001660
domain Fibronectin type III 303 - 528 IPR003961
active_site Tyrosine-protein kinase, active site 740 - 752 IPR008266
domain Tyrosine-protein kinase ephrin type A/B receptor-like 242 - 277 IPR011641
binding_site Protein kinase, ATP binding site 625 - 651 IPR017441
domain Tyrosine-protein kinase, catalytic domain 619 - 878 IPR020635
domain Ephrin receptor, transmembrane domain 542 - 616 IPR027936
domain EphB1 , SAM domain 908 - 975 IPR042819

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Cytoplasm, cytoskeleton, spindle
  • Mediates formation and localizes to cytoplasmic membraneless compartment in response to hyperosmotic stress (PubMed:36318922)
  • Also localizes to the nucleus (By similarity)
  • Localizes to the mitotic spindle during mitosis (By similarity)
PANTHER Family PTHR13902 SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED
PANTHER Subfamily PTHR13902:SF46 SERINE_THREONINE-PROTEIN KINASE WNK1
PANTHER Protein Class non-receptor serine/threonine protein kinase
protein modifying enzyme
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

12 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
chloride channel inhibitor activity Binds to and stops, prevents, or reduces the activity of a chloride channel.
magnesium ion binding Binding to a magnesium (Mg) ion.
phosphatase binding Binding to a phosphatase.
potassium channel inhibitor activity Binds to and stops, prevents, or reduces the activity of a potassium channel.
protein kinase activator activity Binds to and increases the activity of a protein kinase, an enzyme which phosphorylates a protein.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine kinase inhibitor activity Binds to and stops, prevents or reduces the activity of a protein serine/threonine kinase.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

28 GO annotations of biological process

Name Definition
cation homeostasis Any process involved in the maintenance of an internal steady state of cations within an organism or cell.
cellular response to calcium ion Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a calcium ion stimulus.
cellular response to chemokine Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a chemokine stimulus.
chemokine (C-C motif) ligand 21 signaling pathway The series of molecular signals initiated by the binding of the C-C chemokine CCL21 to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
ion homeostasis Any process involved in the maintenance of an internal steady state of ions within an organism or cell.
ion transport The directed movement of charged atoms or small charged molecules into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
lymphocyte migration into lymph node The movement of a lymphocyte within the lymphatic system into a lymph node, and its subsequent positioning within defined functional compartments such as sites of cell activation by antigen.
negative regulation of cell-cell adhesion mediated by integrin Any process that stops, prevents, or reduces the frequency, rate, or extent of cell-cell adhesion mediated by integrin.
negative regulation of GTPase activity Any process that stops or reduces the rate of GTP hydrolysis by a GTPase.
negative regulation of heterotypic cell-cell adhesion Any process that stops, prevents, or reduces the frequency, rate, or extent of heterotypic cell-cell adhesion.
negative regulation of kinase activity Any process that stops, prevents, or reduces the frequency, rate or extent of kinase activity, the catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
negative regulation of leukocyte cell-cell adhesion Any process that stops, prevents or reduces the frequency, rate or extent of leukocyte cell-cell adhesion.
negative regulation of pancreatic juice secretion Any process that decreases the rate, frequency or extent of pancreatic juice secretion, the regulated release of pancreatic juice by the exocrine pancreas into the upper part of the intestine.
negative regulation of sodium ion transport Any process that decreases the frequency, rate or extent of the directed movement of sodium ions (Na+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
peptidyl-threonine phosphorylation The phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
positive regulation of canonical Wnt signaling pathway Any process that increases the rate, frequency, or extent of the Wnt signaling pathway through beta-catenin, the series of molecular signals initiated by binding of a Wnt protein to a frizzled family receptor on the surface of the target cell, followed by propagation of the signal via beta-catenin, and ending with a change in transcription of target genes.
positive regulation of potassium ion import across plasma membrane Any process that activates or increases the frequency, rate or extent of potassium ion import across the plasma membrane.
positive regulation of sodium ion transmembrane transporter activity Any process that activates or increases the frequency, rate or extent of sodium ion transmembrane transporter activity.
positive regulation of systemic arterial blood pressure The process that increases the force with which blood travels through the systemic arterial circulatory system.
positive regulation of T cell chemotaxis Any process that increases the rate, frequency or extent of T cell chemotaxis. T cell chemotaxis is the directed movement of a T cell in response to an external stimulus.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of cation transmembrane transport Any process that modulates the frequency, rate or extent of cation transmembrane transport.
regulation of sodium ion transport Any process that modulates the frequency, rate or extent of the directed movement of sodium ions (Na+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
signal transduction The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
T cell receptor signaling pathway The series of molecular signals initiated by the cross-linking of an antigen receptor on a T cell.

16 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q96J92 WNK4 Serine/threonine-protein kinase WNK4 Homo sapiens (Human) SS
Q9Y3S1 WNK2 Serine/threonine-protein kinase WNK2 Homo sapiens (Human) SS
Q9BYP7 WNK3 Serine/threonine-protein kinase WNK3 Homo sapiens (Human) SS
Q9H4A3 WNK1 Serine/threonine-protein kinase WNK1 Homo sapiens (Human) SS
Q3UH66 Wnk2 Serine/threonine-protein kinase WNK2 Mus musculus (Mouse) SS
Q80UE6 Wnk4 Serine/threonine-protein kinase WNK4 Mus musculus (Mouse) SS
Q80XP9 Wnk3 Serine/threonine-protein kinase WNK3 Mus musculus (Mouse) SS
P83741 Wnk1 Serine/threonine-protein kinase WNK1 Mus musculus (Mouse) SS
Q6R2V0 WNK1 Serine/threonine-protein kinase WNK1 Sus scrofa (Pig) SS
Q7TPK6 Wnk4 Serine/threonine-protein kinase WNK4 Rattus norvegicus (Rat) SS
X5M5N0 wnk-1 Serine/threonine-protein kinase WNK Caenorhabditis elegans SS
Q8RXE5 WNK10 Probable serine/threonine-protein kinase WNK10 Arabidopsis thaliana (Mouse-ear cress) PR
Q944Q0 WNK8 Serine/threonine-protein kinase WNK8 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LVL5 WNK4 Probable serine/threonine-protein kinase WNK4 Arabidopsis thaliana (Mouse-ear cress) PR
Q9STK6 WNK3 Probable serine/threonine-protein kinase WNK3 Arabidopsis thaliana (Mouse-ear cress) PR
Q8LST2 WNK7 Probable serine/threonine-protein kinase WNK7 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MSDGTAEKQS GTPGFLSPPA PVPKNGSSSD SSVGEKLGAA VADSGIGRTE EYRRRRHTMD
70 80 90 100 110 120
KDSRGAAATT TPTEHRFFRR SVICDSNATA LELPGLPLSI PQPSVPAVVP QSAPPEPHRE
130 140 150 160 170 180
ETLTATVASQ VSQQPSAAAS PGEQAVVGSA TATVPSSTSK DRPVSQPSLV GSKEEPPPSR
190 200 210 220 230 240
SGSGSGGASA KEPQEERNQQ QDDIEELETK AVGMSNDGRF LKFDIEIGRG SFKTVYKGLD
250 260 270 280 290 300
TETTVEVAWC ELQDRKLTKS ERQRFKEEAE MLKGLQHPNI VRFYDSWEST VKGKKCIVLV
310 320 330 340 350 360
TELMTSGTLK TYLKRFKVMK IKVLRSWCRQ ILKGLQFLHT RTPPIIHRDL KCDNIFITGP
370 380 390 400 410 420
TGSVKIGDLG LATLKRASFA KSVIGTPEFM APEMYEEKYD ESVDVYAFGM CMLEMATSEY
430 440 450 460 470 480
PYSECQNAAQ IYRRVTSGVK PASFDKVAIP EVKEIIEGCI RQNKDERYSI KDLLNHAFFQ
490 500 510 520 530 540
EETGVRVELA EEDDGEKIAI KLWLRIEDIK KLKGKYKDNE AIEFSFDLER DVPEDVAQEM
550 560 570 580 590 600
VESGYVCEGD HKTMAKAIKD RVSLIKRKRE QRQLVREEQE KRKQEESSFK QQNEQQASVS
610 620 630 640 650 660
QAGIQPLSVA STGIPTAPTT SASVSTQVEP EEPEADQHQQ LQYQQPSISV LSDGTVDSGQ
670 680 690 700 710 720
GSSVFTESRV SSQQTVSYGS QHEQAHSIGT APGHTVSSIQ AQSQPHGVYP PSSMAQGQNQ
730 740 750 760 770 780
GQPSSSLAGV LSSQPVQHPQ QQGIQPTVPP QQAVQYSLPQ AASSSEGTVQ PVSQPQVSAG
790 800 810 820 830 840
TQSSTQGVSQ AAPPEQTPIT QSQPTQPVPL VSSVDSAHSD VASGMSDGNE NAPSSSGRHE
850 860 870 880 890 900
GRTTKRHYRK SVRSRSRHEK TSRPKLRILN VSNKGDRVVE CQLETHNRKM VTFKFDLDGD
910 920 930 940 950 960
NPEEIATIMV NNDFILAIER ESFVAQVREI IEKADEMLSE DVSVEPEGDQ GLESLQGKDD
970 980 990 1000 1010 1020
YGFPGSQKLE GEFKQPIAVS SMPQQIGVPT SSLTQVVHSA GRRFIVSPVP ESRLRESKIF
1030 1040 1050 1060 1070 1080
TSEIPDPVAA STSQGPGMNL SHSASSLSLQ QAFSELKHGQ MTEGPNTAPP NFNHPGPTFS
1090 1100 1110 1120 1130 1140
PFLTSIAGVQ TVAASTPSVS VPITSSPLND ISTSVMQSEG ALPTDKGIGG VTTSTGVVAS
1150 1160 1170 1180 1190 1200
GGLTTLSVSE TPTLSSAVSS STAPAVVTVS TTSQPVQAST SGSIASSTGS FPSGTFSTTT
1210 1220 1230 1240 1250 1260
GTTVSSVAVP NAKPPTVLLQ QVAGNTAGVA IVTSVSTTTP FPAMASQPSL PLGSSTSAPT
1270 1280 1290 1300 1310 1320
LAETVVVSAH SLDKASHSST AGLGLSFCAP SSSSSSGTAV SSSVSQPGIV HPLVISSAIA
1330 1340 1350 1360 1370 1380
STPVLPQPAV PTSTPLLPQV PNIPPLVQPV ANVPAVQQTL IHSQPQPALL PNQPHTHCPE
1390 1400 1410 1420 1430 1440
MDADTQSKAP GIDDIKTLEE KLRSLFSEHS SSGTQHASVS LETPLVVETV TPGIPTTAVA
1450 1460 1470 1480 1490 1500
PSKLMTSTTS TCLPPTNLPL GTAGMPVMPV GTPGQVSTPG THASAPASTA TGAKPGTTPP
1510 1520 1530 1540 1550 1560
KPSLTKTVVP PVGTELSAGT VPCEQLPPFP GPSLIQTQQP LEDLDAQLRR TLSPETIPVT
1570 1580 1590 1600 1610 1620
PAVGPLSTMS STAVTEAGSQ PQKDGTEVHV TASSSGAGVV KMGRFQVSVT MDDAQKERKN
1630 1640 1650 1660 1670 1680
RSEDTKSVHF ESSTSESSVL SSSSPESTLV KPEPNGITVS GISLDVPDST HRTPTPEAKS
1690 1700 1710 1720 1730 1740
ETGQPTKVGR FQVTTTANKV GRFSVSRTED KVTELKKEGP VTSPFRDSEQ TVIPAAIPKK
1750 1760 1770 1780 1790 1800
EKPELAEPSH LNGPSSDLEA AFLSRGGEDG SGSPHSPPHL CSKSLPIQTL SQSLSNSFNS
1810 1820 1830 1840 1850 1860
SYMSSDNESD IEDEDLRLEL RRLREKHLKE IQDLQSRQKH EIESLYTKLG KVPPAVIIPP
1870 1880 1890 1900 1910 1920
AAPLSGRRRR PTKSKGSKSS RSSSLGNKSP QLSGNLSGQS GTSVLNPQQT LHPPGNTPET
1930 1940 1950 1960 1970 1980
GHNQLLQPLK PSPSSDNLYS AFTSDGAISV PSLSAPGQGT SSTNTVGGTV SSQAAQAQPP
1990 2000 2010 2020 2030 2040
AMTSSRKGTF TDDLHKLVDN WARDAMNLSG RRGSKGHMNY EGPGMARKFS APGQLCISMT
2050 2060 2070 2080 2090 2100
SNMGGSTPIS AASATSLGHF TKSMCPPQQY GFPAAPFGTQ WSGTGGPAPQ PLGQFQPVGT
2110 2120
TSLQNFNISN LQKSISNPPG SNLRTT