Q9I8D1
SS
Gene name |
MYO6 (CMY6) |
Protein name |
Unconventional myosin-VI |
Names |
Unconventional myosin-6 |
Species |
Gallus gallus (Chicken) |
KEGG Pathway |
gga:395487 |
EC number |
|
Protein Class |
|
Descriptions
Myosin VI (Myo6) is the only minus-end directed nanomotor on actin and is essential for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. The N-terminal region of the SAH domain folds back on the 3HB domain, thus adopting Myo6 to an elongated conformation that inhibits its activity. Notably, not all partners are capable of relieving the autoinhibition of Myo6. TOM1 and Dab2 are unable to bind the off-state of Myo6, while GIPC1 binds Myo6, releases its autoinhibition, and triggers proximal dimerization. The L926Q deafness mutation also disrupts the autoinhibition of Myo6 and indirectly impairs its proximal dimerization.
Autoinhibitory domains (AIDs)
Target domain |
834-917 (3HB domain) |
Relief mechanism |
Partner binding, Others |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q9I8D1
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9I8D1-F1 | Predicted | AlphaFoldDB |
No variants for Q9I8D1
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q9I8D1 | |||||
No associated diseases with Q9I8D1
6 regional properties for Q9I8D1
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Forkhead-associated (FHA) domain | 470 - 534 | IPR000253 |
| domain | Kinesin motor domain | 3 - 360 | IPR001752 |
| conserved_site | Kinesin motor domain, conserved site | 246 - 257 | IPR019821 |
| domain | Kinesin-like KIF1-type | 748 - 792 | IPR022140 |
| domain | Kinesin-like | 1209 - 1268 | IPR022164 |
| domain | Kinesin-associated | 391 - 468 | IPR032405 |
Functions
22 GO annotations of cellular component
| Name | Definition |
|---|---|
| actin cytoskeleton | The part of the cytoskeleton (the internal framework of a cell) composed of actin and associated proteins. Includes actin cytoskeleton-associated complexes. |
| actin filament | A filamentous structure formed of a two-stranded helical polymer of the protein actin and associated proteins. Actin filaments are a major component of the contractile apparatus of skeletal muscle and the microfilaments of the cytoskeleton of eukaryotic cells. The filaments, comprising polymerized globular actin molecules, appear as flexible structures with a diameter of 5-9 nm. They are organized into a variety of linear bundles, two-dimensional networks, and three dimensional gels. In the cytoskeleton they are most highly concentrated in the cortex of the cell just beneath the plasma membrane. |
| cell cortex | The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins. |
| clathrin-coated pit | A part of the endomembrane system in the form of an invagination of a membrane upon which a clathrin coat forms, and that can be converted by vesicle budding into a clathrin-coated vesicle. Coated pits form on the plasma membrane, where they are involved in receptor-mediated selective transport of many proteins and other macromolecules across the cell membrane, in the trans-Golgi network, and on some endosomes. |
| clathrin-coated vesicle | A vesicle with a coat formed of clathrin connected to the membrane via one of the clathrin adaptor complexes. |
| clathrin-coated vesicle membrane | The lipid bilayer surrounding a clathrin-coated vesicle. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytoplasmic vesicle | A vesicle found in the cytoplasm of a cell. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| endocytic vesicle | A membrane-bounded intracellular vesicle formed by invagination of the plasma membrane around an extracellular substance. Endocytic vesicles fuse with early endosomes to deliver the cargo for further sorting. |
| filamentous actin | A two-stranded helical polymer of the protein actin. |
| filopodium | Thin, stiff, actin-based protrusion extended by the leading edge of a motile cell such as a crawling fibroblast or amoeba, or an axonal or dendritic growth cone, or a dendritic shaft. |
| Golgi apparatus | A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways. |
| microvillus | Thin cylindrical membrane-covered projections on the surface of an animal cell containing a core bundle of actin filaments. Present in especially large numbers on the absorptive surface of intestinal cells. |
| myosin complex | A protein complex, formed of one or more myosin heavy chains plus associated light chains and other proteins, that functions as a molecular motor; uses the energy of ATP hydrolysis to move actin filaments or to move vesicles or other cargo on fixed actin filaments; has magnesium-ATPase activity and binds actin. Myosin classes are distinguished based on sequence features of the motor, or head, domain, but also have distinct tail regions that are believed to bind specific cargoes. |
| nuclear membrane | Either of the lipid bilayers that surround the nucleus and form the nuclear envelope; excludes the intermembrane space. |
| nucleoplasm | That part of the nuclear content other than the chromosomes or the nucleolus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| perinuclear region of cytoplasm | Cytoplasm situated near, or occurring around, the nucleus. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| ruffle | Projection at the leading edge of a crawling cell; the protrusions are supported by a microfilament meshwork. |
| ruffle membrane | The portion of the plasma membrane surrounding a ruffle. |
8 GO annotations of molecular function
| Name | Definition |
|---|---|
| actin binding | Binding to monomeric or multimeric forms of actin, including actin filaments. |
| actin filament binding | Binding to an actin filament, also known as F-actin, a helical filamentous polymer of globular G-actin subunits. |
| ADP binding | Binding to ADP, adenosine 5'-diphosphate. |
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| calmodulin binding | Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states. |
| cytoskeletal motor activity | Generation of force resulting in movement, for example along a microfilament or microtubule, or in torque resulting in membrane scission or rotation of a flagellum. The energy required is obtained either from the hydrolysis of a nucleoside triphosphate or by an electrochemical proton gradient (proton-motive force). |
| microfilament motor activity | A motor activity that generates movement along a microfilament, driven by ATP hydrolysis. |
| plus-end directed microfilament motor activity | A motor activity that generates movement along a microfilament towards the plus end, driven by ATP hydrolysis. The minus end of an actin filament is the end that does not preferentially add actin monomers. |
10 GO annotations of biological process
| Name | Definition |
|---|---|
| actin filament organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments. Includes processes that control the spatial distribution of actin filaments, such as organizing filaments into meshworks, bundles, or other structures, as by cross-linking. |
| actin filament-based movement | Movement of organelles or other particles along actin filaments, or sliding of actin filaments past each other, mediated by motor proteins. |
| DNA damage response, signal transduction by p53 class mediator | A cascade of processes induced by the cell cycle regulator phosphoprotein p53, or an equivalent protein, in response to the detection of DNA damage. |
| endocytosis | A vesicle-mediated transport process in which cells take up external materials or membrane constituents by the invagination of a part of the plasma membrane to form a new membrane-bounded vesicle. |
| inner ear auditory receptor cell differentiation | The process in which a relatively unspecialized inner cell acquires specialized features of an auditory hair cell. |
| inner ear morphogenesis | The process in which the anatomical structures of the inner ear are generated and organized. The inner ear is the structure in vertebrates that contains the organs of balance and hearing. It consists of soft hollow sensory structures (the membranous labyrinth) containing fluid (endolymph) surrounded by fluid (perilymph) and encased in a bony cavity (the bony labyrinth). It consists of two chambers, the sacculus and utriculus, from which arise the cochlea and semicircular canals respectively. |
| intracellular protein transport | The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell. |
| regulation of secretion | Any process that modulates the frequency, rate or extent of the controlled release of a substance from a cell or a tissue. |
| sensory perception of sound | The series of events required for an organism to receive an auditory stimulus, convert it to a molecular signal, and recognize and characterize the signal. Sonic stimuli are detected in the form of vibrations and are processed to form a sound. |
| vesicle transport along actin filament | Movement of a vesicle along an actin filament, mediated by motor proteins. |
6 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| E1BPK6 | MYO6 | Unconventional myosin-VI | Bos taurus (Bovine) | SS |
| Q02440 | MYO5A | Unconventional myosin-Va | Gallus gallus (Chicken) | SS |
| Q9UM54 | MYO6 | Unconventional myosin-VI | Homo sapiens (Human) | EV |
| Q64331 | Myo6 | Unconventional myosin-VI | Mus musculus (Mouse) | SS |
| Q29122 | MYO6 | Unconventional myosin-VI | Sus scrofa (Pig) | SS |
| Q9M2K0 | XI-J | Myosin-16 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MEDGKPVWAP | HPTDGFQMGM | IVDIGTDYLT | IEPLNQKGKT | FQAAINQVFP | AEEDSKKDVE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| DNCSLMYLNE | ATLLHNIKVR | YSKDRIYTYV | ANILIAVNPY | FDIPKFYSSD | AIKKYQGRSL |
| 130 | 140 | 150 | 160 | 170 | 180 |
| GTLPPHVFAI | ADKAYRDMKV | LKMSQSIIVS | GESGAGKTEN | TKFVLRYLTE | SYGTGQDIDD |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RIVEANPLLE | AFGNAKTIRN | NNSSRFGKFV | EIHFNEKNSV | VGGFVSHYLL | EKSRICVQGK |
| 250 | 260 | 270 | 280 | 290 | 300 |
| EERNYHIFYR | LCAGAPEDIR | EKLYLSSPDS | FRYLNRGCTR | YFATKETDKQ | ILQNRKSPEY |
| 310 | 320 | 330 | 340 | 350 | 360 |
| LKAGSLKDPL | LDDHGDFNRM | CTAMKKIGLD | DAEKLDLFRV | VAGVLHLGNI | DFEEAGSTSG |
| 370 | 380 | 390 | 400 | 410 | 420 |
| GCTLKAQSQP | ALECCAALLG | LDEEDLRVSL | TTRVMLTTAG | GAKGTVIKVP | LKVEQANNAR |
| 430 | 440 | 450 | 460 | 470 | 480 |
| DALAKTVYSH | LFDHVVNRVN | QCFPFETSSF | FIGVLDIAGF | EYFEHNSFEQ | FCINYCNEKL |
| 490 | 500 | 510 | 520 | 530 | 540 |
| QQFFNERILK | EEQELYQKEG | LGVNEVRYVD | NQDCIDLIEA | KLIGVLDILD | EENRLPQPSD |
| 550 | 560 | 570 | 580 | 590 | 600 |
| QHFTSVVHQK | HKDHFRLSIP | RKSKLAVHRN | VRDDEGFIIR | HFAGAVCYET | TQFVEKNNDA |
| 610 | 620 | 630 | 640 | 650 | 660 |
| LHMSLESLIC | ESKDKFVRQL | FESNTNNNKD | PKQKAGKLSF | ISVGNKFKTQ | LNLLLEKLHS |
| 670 | 680 | 690 | 700 | 710 | 720 |
| TGSSFIRCIF | PNLKMTSHHF | EGGQILSQLQ | CSGMVSVLDL | MQGGFPSRAS | FHELYNMYKK |
| 730 | 740 | 750 | 760 | 770 | 780 |
| YLPEKLARLD | PRLFCKALFK | ALGLNEIDYK | FGLTKVFFRP | GKFAEFDQIM | KSDPDHLAEL |
| 790 | 800 | 810 | 820 | 830 | 840 |
| VKRVNHWLIC | SRWKKVQWCS | LSVIKLKNKI | KYRASACIKI | QKTIRMWLCK | RKHKPRIDGL |
| 850 | 860 | 870 | 880 | 890 | 900 |
| IKVRTLKKRL | DKFNEVVSAL | KEGKAETSKQ | IKELEYSIDA | SMTKIKTTMM | TREQIMKEYD |
| 910 | 920 | 930 | 940 | 950 | 960 |
| ALVRSSEQLL | SALQKKKQQE | EEAERLRRIQ | EEMEKERKRR | EEEEKRRRKE | EEERRLKSEI |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| EAKRKQEEEE | RKKREEEEKR | IQAEIEAQLA | REREEETQHQ | AILEQERRDR | ELAMRIAQTG |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| AELSTEETKL | DVGLCRANGT | KLQMTAEQMA | KEMSEMLSRG | PAVQATKAAA | GAKKHDLSKW |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| KYAELRDTIN | TSCDIELLAA | CREEFHRRLK | VYHAWKSKNK | KRNAETEQRA | PKSVTDYAQQ |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| NPTAQLPMRQ | QEIEINRQQR | YFRIPFIRPM | DQYKDPQNKK | KGWWYAHFDG | PWIARQMELH |
| 1210 | 1220 | 1230 | 1240 | 1250 | 1260 |
| PDKAPILLVA | GKDDMDMCEL | NLEETGLTRK | RGAEILPRQF | EEIWERCGGI | QYLQNAIESR |
| 1270 | |||||
| QARPTYATAM | LQNLLK |