AiPD: Autoinhibited Protein Database

Q9HBU1

Gene name	BARX1
Protein name	Homeobox protein BarH-like 1
Names
Species	Homo sapiens (Human)
KEGG Pathway	hsa:56033
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

60-254 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

60-254 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

2 structures for Q9HBU1

Entry ID	Method	Resolution	Chain	Position	Source
2DMT	NMR	-	A	133-199	PDB
AF-Q9HBU1-F1	Predicted				AlphaFoldDB

209 variants for Q9HBU1

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs1587810156 CA374334954	3	R>W		No	ClinGen Ensembl
rs895896348 CA196447569	4	P>S		No	ClinGen TOPMed gnomAD
CA374334940 rs1304665974	5	G>V		No	ClinGen gnomAD
rs1483686725 CA374334939	6	E>K		No	ClinGen TOPMed
rs774263956 CA5133429	7	P>L		No	ClinGen ExAC TOPMed gnomAD
COSM3395936 CA374334931 rs1190383513	7	P>S	pancreas [Cosmic]	No	ClinGen cosmic curated gnomAD
CA374334911 rs1262291713	10	A>E		No	ClinGen gnomAD
TCGA novel	10	A>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA374334892 rs1356935133	13	G>C		No	ClinGen gnomAD
rs1356935133 CA374334894	13	G>S		No	ClinGen gnomAD
rs770854053 CA5133428	14	P>T		No	ClinGen ExAC gnomAD
CA374334879 rs1246522511	15	P>R		No	ClinGen gnomAD
rs867917015 CA196447556	16	E>*		No	ClinGen Ensembl
CA374334867 rs1338964571	17	G>D		No	ClinGen gnomAD
rs1412540917 CA374334860	18	C>S		No	ClinGen TOPMed
CA196447550 rs934796935	19	A>P		No	ClinGen TOPMed gnomAD
CA374334856 rs934796935	19	A>T		No	ClinGen TOPMed gnomAD
TCGA novel	19	A>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA374334848 rs1448262683	20	D>A		No	ClinGen TOPMed
CA196447546 rs924748384	22	R>Q		No	ClinGen TOPMed gnomAD
CA374334834 rs1314290662	22	R>W		No	ClinGen TOPMed
CA374334821 rs1288504701	24	H>R		No	ClinGen TOPMed
CA374334817 rs1353455167	25	R>C		No	ClinGen TOPMed
CA374334814 rs1218028254	25	R>H		No	ClinGen TOPMed
rs1327136481 CA374334800	27	R>L		No	ClinGen TOPMed gnomAD
CA196447542 rs866099103	28	S>I		No	ClinGen Ensembl
rs1303027019 CA374334782	30	M>L		No	ClinGen gnomAD
rs1253384506 CA374334772	31	I>V		No	ClinGen TOPMed gnomAD
rs1201657492 CA374334732	36	T>M	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
TCGA novel	37	E>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1182986365 CA374334713	39	P>L		No	ClinGen TOPMed
rs1418324655 CA374334709	40	G>E		No	ClinGen TOPMed gnomAD
rs781194809 CA5133426	40	G>R		No	ClinGen ExAC gnomAD
rs1476079637 CA374334695	42	K>M		No	ClinGen TOPMed gnomAD
CA374334693 rs1373164993	42	K>N		No	ClinGen gnomAD
rs1476079637 CA374334696	42	K>R		No	ClinGen TOPMed gnomAD
CA374334683 rs1489837185	44	A>G		No	ClinGen TOPMed gnomAD
CA196447525 rs560200241	44	A>T		No	ClinGen 1000Genomes gnomAD
rs1489837185 CA374334682	44	A>V		No	ClinGen TOPMed gnomAD
rs1175286609 CA374334678	45	A>E		No	ClinGen TOPMed
rs1271031550 CA374334681	45	A>T		No	ClinGen TOPMed gnomAD
CA374334671 rs1563990316	46	P>H		No	ClinGen Ensembl
rs1437054497 CA374334669	47	A>T		No	ClinGen gnomAD
rs191789925 VAR_010927 CA5133425	48	A>T		No	ClinGen UniProt 1000Genomes ExAC TOPMed dbSNP gnomAD
CA374334656 rs1216574487	49	A>T		No	ClinGen gnomAD
rs1340093500 CA374334652	49	A>V		No	ClinGen gnomAD
rs576837358 CA5133424	51	A>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA374334643 rs1347925732	51	A>V		No	ClinGen gnomAD
rs1441897044 CA374334635	52	A>V		No	ClinGen gnomAD
CA374334624 rs1353143378	54	G>A		No	ClinGen gnomAD
rs758702906 CA5133422	56	L>Q		No	ClinGen ExAC gnomAD
rs1400661440 CA374334601	58	K>R		No	ClinGen TOPMed gnomAD
CA374334597 rs1587810002	59	F>L		No	ClinGen Ensembl
rs1273016991 CA374334589	60	G>S		No	ClinGen TOPMed
rs1479197274 CA374334580	61	V>E		No	ClinGen TOPMed gnomAD
CA374334578 rs1479197274	61	V>G		No	ClinGen TOPMed gnomAD
CA196447495 rs964462092	62	Q>R		No	ClinGen TOPMed
rs1200103921 CA374334566	63	A>G		No	ClinGen gnomAD
rs941962960 CA374334551	66	A>E		No	ClinGen gnomAD
CA196447492 rs941962960	66	A>G		No	ClinGen gnomAD
CA374334544 rs1449899122	67	A>V		No	ClinGen TOPMed
CA374334541 rs1563990263	68	R>Q		No	ClinGen Ensembl
TCGA novel	69	P>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1198280925 CA374334510	72	S>R		No	ClinGen TOPMed
rs773164189 CA5133406	75	A>D		No	ClinGen ExAC gnomAD
rs773164189 CA374334479	75	A>V		No	ClinGen ExAC gnomAD
CA374334477 rs1313862806	76	V>M		No	ClinGen TOPMed gnomAD
CA374334463 rs1302918191	78	K>R		No	ClinGen gnomAD
rs1489234016 CA374334451	80	E>*		No	ClinGen gnomAD
rs1489234016 CA374334453	80	E>K		No	ClinGen gnomAD
rs867705180 CA196446454	81	Q>*		No	ClinGen Ensembl
rs865878957 CA196446450	84	V>L		No	ClinGen Ensembl
CA374334414 rs1161255040	85	F>L		No	ClinGen gnomAD
rs1171309735 CA374334412	86	K>E		No	ClinGen TOPMed
CA5133403 rs747038748	86	K>N		No	ClinGen ExAC gnomAD
CA374334397 rs1587809079	88	P>A		No	ClinGen Ensembl
CA5133402 rs780245526	88	P>Q		No	ClinGen ExAC gnomAD
rs541371621 CA374334392	89	L>V		No	ClinGen TOPMed
rs746115723 CA5133400	92	L>P		No	ClinGen ExAC TOPMed gnomAD
CA374334370 rs1185977992	93	G>C		No	ClinGen gnomAD
rs1484955097 CA374334369	93	G>D		No	ClinGen gnomAD
rs1185977992 CA374334371	93	G>R		No	ClinGen gnomAD
CA5133399 rs549526966	94	C>R		No	ClinGen ExAC TOPMed gnomAD
rs532945486 CA5133398	95	S>L		No	ClinGen 1000Genomes ExAC gnomAD
CA374334358 rs1442164135	95	S>P		No	ClinGen TOPMed
rs1343899 CA196446417	96	G>R		No	ClinGen Ensembl
CA374334346 rs1379510866	97	L>P		No	ClinGen TOPMed
CA5133396 rs778400548	98	S>I		No	ClinGen ExAC TOPMed gnomAD
CA196446406 rs778400548	98	S>N		No	ClinGen ExAC TOPMed gnomAD
rs1361139386 CA374334339	98	S>R		No	ClinGen TOPMed gnomAD
rs1313049531 CA374334333	99	S>F		No	ClinGen gnomAD
CA5133395 rs756740714	100	A>G		No	ClinGen ExAC TOPMed gnomAD
rs1213446917 CA374334330	100	A>T		No	ClinGen gnomAD
TCGA novel	101	L>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs45527632 CA196446393	103	A>T		No	ClinGen Ensembl
CA374334308 rs1245867728	104	A>S		No	ClinGen TOPMed gnomAD
rs767061973 CA5133393	105	G>R		No	ClinGen ExAC TOPMed gnomAD
rs544075964 CA5133392	106	P>A		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA5133391 rs544075964	106	P>S		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA5133389 CA374334295 rs531602020	107	G>R		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs531602020 CA5133390	107	G>W		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1421759690 CA374334280	110	G>S		No	ClinGen TOPMed gnomAD
CA374334273 rs201946673	111	A>S		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA5133387 rs201946673	111	A>T		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1044700919 CA196446345	112	A>T		No	ClinGen TOPMed
CA374334266 rs1267457561	112	A>V		No	ClinGen gnomAD
rs1403090266 CA374334257	114	A>T		No	ClinGen TOPMed
CA5133386 rs761812989	116	H>P		No	ClinGen ExAC gnomAD
rs772214631 CA5133384	117	L>M		No	ClinGen ExAC gnomAD
rs1218772762 CA374334229	118	P>L		No	ClinGen gnomAD
CA5133381 rs771325755	121	L>F		No	ClinGen ExAC gnomAD
rs1391213209 CA374334202	122	Q>H		No	ClinGen gnomAD
rs373384950 CA196446326	123	L>F		No	ClinGen ESP TOPMed gnomAD
rs1460666524 CA374334192 COSM1184445	124	R>H	large_intestine [Cosmic]	No	ClinGen cosmic curated gnomAD
CA5133380 rs369928804	129	A>S		No	ClinGen ESP ExAC gnomAD
rs1427777963 CA374334154	130	A>E		No	ClinGen gnomAD
rs868608877 CA374334148	131	G>D		No	ClinGen gnomAD
CA196446306 rs868608877	131	G>V		No	ClinGen gnomAD
rs778351778 CA5133379	132	P>R		No	ClinGen ExAC gnomAD
rs770498173 CA196446301	134	E>D		No	ClinGen Ensembl
rs748707341 CA5133377	136	G>S		No	ClinGen ExAC gnomAD
rs780522907 CA5133376	137	T>I		No	ClinGen ExAC TOPMed gnomAD
rs780522907 CA374334114	137	T>S		No	ClinGen ExAC TOPMed gnomAD
rs1198689451 CA374334099	139	A>V		No	ClinGen gnomAD
CA5133375 rs754547962	140	K>R		No	ClinGen ExAC gnomAD
CA374334080 rs1261122752	142	G>E		No	ClinGen gnomAD
TCGA novel	142	G>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA374334074 rs1331406485	143	R>H		No	ClinGen TOPMed gnomAD
rs866954519 CA196446272	145	S>I		No	ClinGen gnomAD
rs866954519 CA374334063	145	S>N		No	ClinGen gnomAD
rs1389519411 CA374334055	146	R>H		No	ClinGen gnomAD
CA374334027 rs1332350470	151	E>K		No	ClinGen gnomAD
CA374334012 rs1179141312	153	Q>*		No	ClinGen gnomAD
rs1466998124 CA374334007	153	Q>H		No	ClinGen gnomAD
TCGA novel	158	E>D	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs765995311 CA5133373	162	E>A		No	ClinGen ExAC TOPMed gnomAD
CA374333929 rs142710643	164	Q>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1239576202 CA374333936	164	Q>K		No	ClinGen gnomAD
rs750149824 CA5133371	169	T>K		No	ClinGen ExAC gnomAD
rs750149824 COSM1111311 CA374333896	169	T>M	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ExAC NCI-TCGA gnomAD
rs531210852 CA196446241	170	P>L		No	ClinGen gnomAD
rs778532537 CA5133352	173	I>T		No	ClinGen ExAC gnomAD
rs1231334745 CA374333848	175	L>I		No	ClinGen TOPMed
CA5133349 rs556916619	181	L>M		No	ClinGen 1000Genomes ExAC gnomAD
CA5133348 rs201922522	184	L>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA374333784 rs1263813289	185	Q>*		No	ClinGen gnomAD
TCGA novel	187	K>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA5133347 rs751625001	193	R>W	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
CA196446152 rs904775426	194	R>M		No	ClinGen Ensembl
CA374333713 rs1198131040	195	M>L		No	ClinGen gnomAD
rs1382974696 CA374333672	200	I>V		No	ClinGen gnomAD
rs745365151 CA5133318	201	V>M		No	ClinGen ExAC gnomAD
rs557180708 CA196445848	203	Q>R		No	ClinGen gnomAD
CA374333635 rs1587808587	204	G>C		No	ClinGen Ensembl
rs770548524 CA5133316	204	G>D		No	ClinGen ExAC TOPMed gnomAD
CA374333627 rs1175534327	205	G>D		No	ClinGen gnomAD
rs1400402143 CA374333630	205	G>S		No	ClinGen gnomAD
CA5133315 rs748908921	206	G>S		No	ClinGen ExAC TOPMed gnomAD
rs957172729 CA196445824	208	E>A		No	ClinGen TOPMed gnomAD
rs957172729 CA374333611	208	E>G		No	ClinGen TOPMed gnomAD
CA374333596 rs1180771426	210	P>L		No	ClinGen gnomAD
CA5133314 rs149189558	211	T>I		No	ClinGen ESP ExAC TOPMed gnomAD
rs1239629658 CA374333594	211	T>P		No	ClinGen TOPMed
TCGA novel	215	G>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA374333560 rs1235663514	216	R>Q	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
rs1279996964 CA374333561	216	R>W		No	ClinGen gnomAD
TCGA novel	217	P>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs948370232 CA196445817	218	K>R		No	ClinGen TOPMed gnomAD
CA5133310 rs755024473	220	N>I		No	ClinGen ExAC gnomAD
CA5133309 rs145379597	222	I>V		No	ClinGen ESP ExAC TOPMed gnomAD
CA374333517 rs1157606968	223	P>S		No	ClinGen TOPMed
rs992336948 CA196445787	224	T>A		No	ClinGen gnomAD
rs757450124 CA5133307	224	T>M		No	ClinGen ExAC TOPMed gnomAD
CA5133308 rs757450124	224	T>R		No	ClinGen ExAC TOPMed gnomAD
rs746558122 CA196445762	225	S>R		No	ClinGen Ensembl
CA196445760 rs961583468	226	E>Q		No	ClinGen TOPMed
CA374333493 rs1324622909	227	Q>K		No	ClinGen gnomAD
rs201122153 CA5133305	228	L>F		No	ClinGen ESP ExAC TOPMed gnomAD
rs201122153 CA5133306	228	L>V		No	ClinGen ESP ExAC TOPMed gnomAD
TCGA novel	229	T>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs560651229 CA5133304	230	E>K		No	ClinGen 1000Genomes ExAC gnomAD
CA196445750 rs972503300	233	R>C		No	ClinGen TOPMed
CA374333449 rs1378266524	233	R>L		No	ClinGen TOPMed gnomAD
CA374333453 rs972503300	233	R>S		No	ClinGen TOPMed
CA374333446 rs1446509008	234	A>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
rs1181808628 CA374333434	236	D>N		No	ClinGen gnomAD
TCGA novel	238	E>D	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA5133300 rs773850927	238	E>K		No	ClinGen ExAC TOPMed gnomAD
rs1215715737 CA374333411	239	K>*		No	ClinGen gnomAD
CA374333401 rs1288596242	240	P>L		No	ClinGen gnomAD
rs770372550 CA5133299	240	P>T		No	ClinGen ExAC gnomAD
rs1313975275 CA374333398	241	A>E		No	ClinGen TOPMed gnomAD
rs758410858 CA196445734	241	A>T		No	ClinGen TOPMed gnomAD
rs1313975275 CA374333396	241	A>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
rs1587808480 CA374333382	243	V>G		No	ClinGen Ensembl
CA374333385 rs1226080454	243	V>L		No	ClinGen gnomAD
CA374333378 rs1239139496	244	P>L		No	ClinGen TOPMed
CA374333367 rs1429948249	246	E>*		No	ClinGen gnomAD
CA374333357 rs1423783980	247	P>R		No	ClinGen gnomAD
CA374333354 rs1358624725	248	S>G		No	ClinGen gnomAD
rs772711999 CA5133297	248	S>N		No	ClinGen ExAC gnomAD
CA374333346 rs1456235522	249	D>H		No	ClinGen TOPMed
CA374333347 rs1456235522	249	D>N		No	ClinGen TOPMed
CA374333348 rs1456235522	249	D>Y		No	ClinGen TOPMed
CA374333334 rs1365144935	250	R>S		No	ClinGen gnomAD
CA374333339 rs1171882411	250	R>W		No	ClinGen gnomAD
rs374624014 CA196445726	252	R>H		No	ClinGen ESP TOPMed gnomAD
rs374624014 CA196445722	252	R>L		No	ClinGen ESP TOPMed gnomAD
rs199527359 CA5133294	253	E>K		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD

No associated diseases with Q9HBU1

5 regional properties for Q9HBU1

Type	Name	Position	InterPro Accession
conserved_site	Helix-turn-helix motif	171 - 196	IPR000047
domain	Homeobox domain	140 - 204	IPR001356
conserved_site	Homeobox, conserved site	175 - 198	IPR017970
domain	Homeobox domain, metazoa	164 - 175	IPR020479-1
domain	Homeobox domain, metazoa	179 - 198	IPR020479-2

Functions

		Description
EC Number
Subcellular Localization	Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

2 GO annotations of cellular component

Name	Definition
chromatin	The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

3 GO annotations of molecular function

Name	Definition
DNA-binding transcription factor activity	A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
DNA-binding transcription factor activity, RNA polymerase II-specific	A DNA-binding transcription factor activity that modulates the transcription of specific gene sets transcribed by RNA polymerase II.
RNA polymerase II transcription regulatory region sequence-specific DNA binding	Binding to a specific sequence of DNA that is part of a regulatory region that controls the transcription of a gene or cistron by RNA polymerase II.

7 GO annotations of biological process

Name	Definition
anterior/posterior pattern specification	The regionalization process in which specific areas of cell differentiation are determined along the anterior-posterior axis. The anterior-posterior axis is defined by a line that runs from the head or mouth of an organism to the tail or opposite end of the organism.
digestive system development	The process whose specific outcome is the progression of the digestive system over time, from its formation to the mature structure. The digestive system is the entire structure in which digestion takes place. Digestion is all of the physical, chemical, and biochemical processes carried out by multicellular organisms to break down ingested nutrients into components that may be easily absorbed and directed into metabolism.
endothelial cell differentiation	The process in which a mesodermal, bone marrow or neural crest cell acquires specialized features of an endothelial cell, a thin flattened cell. A layer of such cells lines the inside surfaces of body cavities, blood vessels, and lymph vessels, making up the endothelium.
negative regulation of Wnt signaling pathway	Any process that stops, prevents, or reduces the frequency, rate or extent of the Wnt signaling pathway.
regulation of transcription by RNA polymerase II	Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
spleen development	The process whose specific outcome is the progression of the spleen over time, from its formation to the mature structure. The spleen is a large vascular lymphatic organ composed of white and red pulp, involved both in hemopoietic and immune system functions.
Wnt signaling pathway	The series of molecular signals initiated by binding of a Wnt protein to a frizzled family receptor on the surface of the target cell and ending with a change in cell state.

7 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q9DED6	BARX1B	Homeobox protein BarH-like 1b	Gallus gallus (Chicken)	PR
Q6RFL5	BSX	Brain-specific homeobox protein homolog	Gallus gallus (Chicken)	PR
Q9W6D8	BARX1	Homeobox protein BarH-like 1	Gallus gallus (Chicken)	PR
Q9UMQ3	BARX2	Homeobox protein BarH-like 2	Homo sapiens (Human)	PR
Q3C1V8	BSX	Brain-specific homeobox protein homolog	Homo sapiens (Human)	PR
Q9ER42	Barx1	Homeobox protein BarH-like 1	Mus musculus (Mouse)	PR
Q810B3	Bsx	Brain-specific homeobox protein homolog	Mus musculus (Mouse)	PR

10	20	30	40	50	60
MQRPGEPGAA	RFGPPEGCAD	HRPHRYRSFM	IEEILTEPPG	PKGAAPAAAA	AAAGELLKFG
70	80	90	100	110	120
VQALLAARPF	HSHLAVLKAE	QAAVFKFPLA	PLGCSGLSSA	LLAAGPGLPG	AAGAPHLPLE
130	140	150	160	170	180
LQLRGKLEAA	GPGEPGTKAK	KGRRSRTVFT	ELQLMGLEKR	FEKQKYLSTP	DRIDLAESLG
190	200	210	220	230	240
LSQLQVKTWY	QNRRMKWKKI	VLQGGGLESP	TKPKGRPKKN	SIPTSEQLTE	QERAKDAEKP
250
AEVPGEPSDR	SRED