Q9FJJ5
Gene name |
EB1B |
Protein name |
Microtubule-associated protein RP/EB family member 1B |
Names |
APC-binding protein EB1B, End-binding protein 1, AtEB1, End-binding protein 1B, AtEB1B, Protein ATEB1 homolog 2, ATEB1H2 |
Species |
Arabidopsis thaliana (Mouse-ear cress) |
KEGG Pathway |
ath:AT5G62500 |
EC number |
|
Protein Class |
MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER (PTHR10623) |
Descriptions
End-binding 1 (EB1) proteins are plus-end-tracking proteins that localize to growing microtubule (MT) plus ends. In Arabidopsis thaliana, EB1 proteins contain an N-terminal calponin homology (CH) domain and a composite C-terminal (CC) domain that mediate MT binding and dimerization, respectively. Furthermore, the C-terminal tail of the EB1B subgroup autoinhibits EB1’s ability to assemble MTs, possibly by interacting with the N-terminal MT-binding CH domain.
Autoinhibitory domains (AIDs)
Target domain |
1-120 (CH domain) |
Relief mechanism |
Partner binding |
Assay |
Deletion assay, Mutagenesis experiment |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q9FJJ5
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9FJJ5-F1 | Predicted | AlphaFoldDB |
9 variants for Q9FJJ5
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| tmp_5_25094052_C_A | 100 | P>Q | No | 1000Genomes | |
| tmp_5_25094259_A_G | 139 | K>R | No | 1000Genomes | |
| tmp_5_25094268_A_C | 142 | K>T | No | 1000Genomes | |
| ENSVATH12907296 | 168 | A>T | No | 1000Genomes | |
| ENSVATH03464680 | 188 | V>M | No | 1000Genomes | |
| ENSVATH07467977 | 228 | L>I | No | 1000Genomes | |
| ENSVATH03464686 | 244 | T>I | No | 1000Genomes | |
| ENSVATH00747957 | 266 | G>S | No | 1000Genomes | |
| ENSVATH03464687 | 285 | A>E | No | 1000Genomes |
No associated diseases with Q9FJJ5
Functions
| Description | ||
|---|---|---|
| EC Number | ||
| Subcellular Localization |
|
|
| PANTHER Family | PTHR10623 | MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER |
| PANTHER Subfamily | PTHR10623:SF29 | MICROTUBULE-ASSOCIATED PROTEIN RP_EB FAMILY MEMBER 1B |
| PANTHER Protein Class | non-motor microtubule binding protein | |
| PANTHER Pathway Category | No pathway information available | |
10 GO annotations of cellular component
| Name | Definition |
|---|---|
| cortical microtubule, transverse to long axis | Arrays of microtubules underlying and connected to the plasma membrane, in the cortical cytosol, oriented mainly with their axes transverse to the long axis of the cell (and root in plants). In plants it influences the direction of cellulose microfibril deposition. |
| cytoplasmic microtubule | Any microtubule in the cytoplasm of a cell. |
| microtubule | Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle. |
| microtubule organizing center | An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides. |
| microtubule plus-end | The growing (plus) end of a microtubule. In vitro, microtubules polymerize more quickly at the plus end than at the minus end. In vivo, microtubule growth occurs only at the plus end, and the plus end switches between periods of growth and shortening, a behavior known as dynamic instability. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| phragmoplast | Fibrous structure (light microscope view) that arises between the daughter nuclei at telophase and within which the initial partition (cell plate), dividing the mother cell in two (cytokinesis), is formed. Appears at first as a spindle connected to the two nuclei, but later spreads laterally in the form of a ring. Consists of microtubules. |
| spindle | The array of microtubules and associated molecules that forms between opposite poles of a eukaryotic cell during mitosis or meiosis and serves to move the duplicated chromosomes apart. |
| spindle midzone | The area in the center of the spindle where the spindle microtubules from opposite poles overlap. |
| spindle pole | Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules. |
1 GO annotations of molecular function
| Name | Definition |
|---|---|
| microtubule plus-end binding | Binding to the plus end of a microtubule. |
7 GO annotations of biological process
| Name | Definition |
|---|---|
| cell division | The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells. |
| microtubule bundle formation | A process that results in a parallel arrangement of microtubules. |
| positive gravitropism | The orientation of plant parts towards gravity. |
| protein localization to microtubule plus-end | A process in which a protein is transported to, or maintained in, a location at a microtubule plus-end. |
| regulation of microtubule polymerization or depolymerization | Any process that modulates the frequency, rate or extent of microtubule polymerization or depolymerization by the addition or removal of tubulin heterodimers from a microtubule. |
| spindle assembly | The aggregation, arrangement and bonding together of a set of components to form the spindle, the array of microtubules and associated molecules that serves to move duplicated chromosomes apart. |
| thigmotropism | The movement of an organism, or part of an organism, such as leaves or tendrils, in response to a touch stimulus, usually toward or away from it. |
12 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q3ZBD9 | MAPRE1 | Microtubule-associated protein RP/EB family member 1 | Bos taurus (Bovine) | SS |
| Q5ZLC7 | MAPRE1 | Microtubule-associated protein RP/EB family member 1 | Gallus gallus (Chicken) | SS |
| Q15691 | MAPRE1 | Microtubule-associated protein RP/EB family member 1 | Homo sapiens (Human) | EV |
| Q15555 | MAPRE2 | Microtubule-associated protein RP/EB family member 2 | Homo sapiens (Human) | PR |
| Q9UPY8 | MAPRE3 | Microtubule-associated protein RP/EB family member 3 | Homo sapiens (Human) | SS |
| Q61166 | Mapre1 | Microtubule-associated protein RP/EB family member 1 | Mus musculus (Mouse) | SS |
| Q6PER3 | Mapre3 | Microtubule-associated protein RP/EB family member 3 | Mus musculus (Mouse) | SS |
| Q8R001 | Mapre2 | Microtubule-associated protein RP/EB family member 2 | Mus musculus (Mouse) | PR |
| Q5XIT1 | Mapre3 | Microtubule-associated protein RP/EB family member 3 | Rattus norvegicus (Rat) | SS |
| Q66HR2 | Mapre1 | Microtubule-associated protein RP/EB family member 1 | Rattus norvegicus (Rat) | SS |
| Q7XJ60 | EB1A | Microtubule-associated protein RP/EB family member 1A | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q6P848 | mapre1 | Microtubule-associated protein RP/EB family member 1 | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MATNIGMMDS | AYFVGRNEIL | SWINDRLHLN | LSRIEEAASG | AVQCQMLDMT | FPGVVPMHKV |
| 70 | 80 | 90 | 100 | 110 | 120 |
| NFEAKNEYEM | IQNYKVMQEV | FTKLKITKPL | EVNRLVKGRP | LDNLEFLQWL | KRFCDSINGG |
| 130 | 140 | 150 | 160 | 170 | 180 |
| IMNENYNPVE | RRSRGGREKS | VKGSSKISKS | LQTNNMHHPP | VATSNKPAGP | KQAKSHGIGG |
| 190 | 200 | 210 | 220 | 230 | 240 |
| GSNSSAEVQA | LSKEVEDLKV | SVDLLEKERD | FYFSKLRDIE | ILCQTPELDD | LPIVVAVKKI |
| 250 | 260 | 270 | 280 | 290 | |
| LYATDANESV | LEEAQECLNQ | SLGLEGYEEE | GKEEEEEEEE | EEEEAAAAAE | TQT |