Q9FJ09
SS
Gene name |
IMPA5 (At5g49310, K21P3.21) |
Protein name |
Importin subunit alpha-5 |
Names |
IMPa-5 , Importin subunit alpha |
Species |
Arabidopsis thaliana (Mouse-ear cress) |
KEGG Pathway |
ath:AT5G49310 |
EC number |
|
Protein Class |
|
Descriptions
Importin-α is a nuclear import receptor that facilitates the transport of proteins with nuclear localization sequences (NLSs) into the nucleus. It is autoinhibited by its N-terminal region, which occupies the NLS-binding site, preventing unnecessary binding and import activity. Importin-α is autoinhibited in the absence of importin-β and the binding of importin-β alleviates this autoinhibition, which displaces the autoinhibitory sequence.
Autoinhibitory domains (AIDs)
Target domain |
136-228 (Major NLS-binding site);304-393 (Minor NLS-binding site) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Fontes MR et al. (2000) "Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha", Journal of molecular biology, 297, 1183-94
- Fontes MR et al. (2003) "Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha", The Journal of biological chemistry, 278, 27981-7
- Catimel B et al. (2001) "Biophysical characterization of interactions involving importin-alpha during nuclear import", The Journal of biological chemistry, 276, 34189-98
- Pumroy RA et al. (2015) "Molecular determinants for nuclear import of influenza A PB2 by importin α isoforms 3 and 7", Structure (London, England : 1993), 23, 374-84
Autoinhibited structure
Activated structure
1 structures for Q9FJ09
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9FJ09-F1 | Predicted | AlphaFoldDB |
74 variants for Q9FJ09
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| tmp_5_19994491_G_A | 9 | T>I | No | 1000Genomes | |
| tmp_5_19994489_C_T | 10 | E>K | No | 1000Genomes | |
| ENSVATH00721771 | 14 | I>S | No | 1000Genomes | |
| ENSVATH07380235 | 31 | D>E | No | 1000Genomes | |
| ENSVATH00721767 | 59 | K>Q | No | 1000Genomes | |
| ENSVATH07380225 | 83 | D>Y | No | 1000Genomes | |
| ENSVATH00721763 | 88 | L>F | No | 1000Genomes | |
| tmp_5_19994125_T_G | 89 | E>A | No | 1000Genomes | |
| ENSVATH00721762 | 92 | T>S | No | 1000Genomes | |
| ENSVATH07380224 | 97 | V>I | No | 1000Genomes | |
| ENSVATH07380219 | 106 | T>I | No | 1000Genomes | |
| ENSVATH07380218 | 107 | D>E | No | 1000Genomes | |
| ENSVATH07380217 | 112 | S>C | No | 1000Genomes | |
| ENSVATH07380212 | 137 | A>V | No | 1000Genomes | |
| ENSVATH07380211 | 142 | A>V | No | 1000Genomes | |
| tmp_5_19993773_T_C | 149 | T>A | No | 1000Genomes | |
| ENSVATH07380208 | 157 | V>A | No | 1000Genomes | |
| ENSVATH00721760 | 159 | P>S | No | 1000Genomes | |
| ENSVATH07380207 | 160 | L>I | No | 1000Genomes | |
| tmp_5_19993734_C_T | 162 | V>I | No | 1000Genomes | |
| ENSVATH00721759 | 168 | P>T | No | 1000Genomes | |
| ENSVATH07380198 | 200 | F>L | No | 1000Genomes | |
| ENSVATH07380197 | 213 | L>* | No | 1000Genomes | |
| ENSVATH07380196 | 214 | S>L | No | 1000Genomes | |
| ENSVATH00721757 | 217 | R>K | No | 1000Genomes | |
| ENSVATH07380195 | 228 | R>C | No | 1000Genomes | |
| ENSVATH12655302 | 228 | R>H | No | 1000Genomes | |
| ENSVATH07380194 | 229 | G>S | No | 1000Genomes | |
| ENSVATH07380193 | 230 | K>* | No | 1000Genomes | |
| ENSVATH07380191 | 233 | P>L | No | 1000Genomes | |
| ENSVATH07380191 | 233 | P>Q | No | 1000Genomes | |
| ENSVATH00721756 | 241 | V>A | No | 1000Genomes | |
| ENSVATH07380186 | 241 | V>F | No | 1000Genomes | |
| tmp_5_19993334_A_C | 245 | L>R | No | 1000Genomes | |
| ENSVATH07380184 | 250 | Y>N | No | 1000Genomes | |
| ENSVATH12655297 | 263 | A>G | No | 1000Genomes | |
| ENSVATH03396701 | 268 | S>F | No | 1000Genomes | |
| tmp_5_19993236_C_T | 278 | V>M | No | 1000Genomes | |
| ENSVATH07380183 | 279 | I>N | No | 1000Genomes | |
| ENSVATH07380181 | 292 | Q>H | No | 1000Genomes | |
| ENSVATH07380180 | 296 | P>A | No | 1000Genomes | |
| tmp_5_19993090_C_T | 298 | V>I | No | 1000Genomes | |
| ENSVATH07380178 | 304 | R>L | No | 1000Genomes | |
|
ENSVATH03396695 ENSVATH03396696 |
305 | C>S | No | 1000Genomes | |
| ENSVATH07380177 | 309 | I>V | No | 1000Genomes | |
| ENSVATH00721754 | 313 | N>S | No | 1000Genomes | |
| ENSVATH03396694 | 318 | H>R | No | 1000Genomes | |
| tmp_5_19992794_C_A | 323 | C>F | No | 1000Genomes | |
| ENSVATH07380172 | 330 | A>T | No | 1000Genomes | |
| tmp_5_19992735_T_A | 343 | R>W | No | 1000Genomes | |
| tmp_5_19992679_C_A | 361 | Q>H | No | 1000Genomes | |
| ENSVATH03396686 | 371 | S>T | No | 1000Genomes | |
| tmp_5_19992468_C_A | 397 | G>V | No | 1000Genomes | |
| ENSVATH03396685 | 400 | P>L | No | 1000Genomes | |
| tmp_5_19992457_T_C | 401 | N>D | No | 1000Genomes | |
| ENSVATH14616439 | 404 | K>T | No | 1000Genomes | |
| tmp_5_19992302_C_T | 425 | R>K | No | 1000Genomes | |
| tmp_5_19992275_A_T | 434 | L>Q | No | 1000Genomes | |
| ENSVATH07380162 | 435 | E>D | No | 1000Genomes | |
| tmp_5_19992272_T_C | 435 | E>G | No | 1000Genomes | |
| ENSVATH00721747 | 439 | I>M | No | 1000Genomes | |
| ENSVATH07380161 | 443 | V>G | No | 1000Genomes | |
| tmp_5_19992242_T_A | 445 | K>M | No | 1000Genomes | |
| ENSVATH00721745 | 451 | N>K | No | 1000Genomes | |
| ENSVATH12655255 | 456 | M>I | No | 1000Genomes | |
| ENSVATH07380157 | 486 | Q>H | No | 1000Genomes | |
| ENSVATH00721744 | 489 | G>W | No | 1000Genomes | |
| tmp_5_19992094_A_T | 494 | D>E | No | 1000Genomes | |
| ENSVATH00721743 | 496 | R>G | No | 1000Genomes | |
| ENSVATH00721742 | 508 | C>R | No | 1000Genomes | |
| ENSVATH03396684 | 516 | D>A | No | 1000Genomes | |
| ENSVATH03396684 | 516 | D>G | No | 1000Genomes | |
| tmp_5_19992030_C_G | 516 | D>H | No | 1000Genomes | |
| ENSVATH00721741 | 518 | S>T | No | 1000Genomes |
No associated diseases with Q9FJ09
8 regional properties for Q9FJ09
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| repeat | Armadillo | 102 - 185 | IPR000225-1 |
| repeat | Armadillo | 186 - 228 | IPR000225-2 |
| repeat | Armadillo | 230 - 311 | IPR000225-3 |
| repeat | Armadillo | 313 - 354 | IPR000225-4 |
| repeat | Armadillo | 356 - 396 | IPR000225-5 |
| repeat | Armadillo | 399 - 439 | IPR000225-6 |
| domain | Importin-alpha, importin-beta-binding domain | 1 - 92 | IPR002652 |
| repeat | Atypical Arm repeat | 451 - 495 | IPR032413 |
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| nuclear envelope | The double lipid bilayer enclosing the nucleus and separating its contents from the rest of the cytoplasm; includes the intermembrane space, a gap of width 20-40 nm (also called the perinuclear space). |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| nuclear import signal receptor activity | Combining with a nuclear import signal (NIS) on a cargo to be transported, to mediate transport of the cargo through the nuclear pore, from the cytoplasm to the nuclear lumen. The cargo can be either a RNA or a protein. |
| nuclear localization sequence binding | Binding to a nuclear localization sequence, a specific peptide sequence that acts as a signal to localize the protein within the nucleus. |
1 GO annotations of biological process
| Name | Definition |
|---|---|
| NLS-bearing protein import into nucleus | The directed movement of a protein bearing a nuclear localization signal (NLS) from the cytoplasm into the nucleus, across the nuclear envelope. |
30 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q02821 | SRP1 | Importin subunit alpha | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | SS |
| A2VE08 | KPNA1 | Importin subunit alpha-5 | Bos taurus (Bovine) | SS |
| C1JZ66 | KPNA7 | Importin subunit alpha-8 | Bos taurus (Bovine) | PR |
| Q0V7M0 | KPNA6 | Importin subunit alpha-7 | Bos taurus (Bovine) | SS |
| Q5ZML1 | KPNA1 | Importin subunit alpha-5 | Gallus gallus (Chicken) | SS |
| P52295 | Pen | Importin subunit alpha | Drosophila melanogaster (Fruit fly) | SS |
| A9QM74 | KPNA7 | Importin subunit alpha-8 | Homo sapiens (Human) | EV |
| O00505 | KPNA3 | Importin subunit alpha-4 | Homo sapiens (Human) | SS |
| O00629 | KPNA4 | Importin subunit alpha-3 | Homo sapiens (Human) | SS |
| O15131 | KPNA5 | Importin subunit alpha-6 | Homo sapiens (Human) | SS |
| O60684 | KPNA6 | Importin subunit alpha-7 | Homo sapiens (Human) | SS |
| P52292 | KPNA2 | Importin subunit alpha-1 | Homo sapiens (Human) | EV |
| P52294 | KPNA1 | Importin subunit alpha-5 | Homo sapiens (Human) | SS |
| P52293 | Kpna2 | Importin subunit alpha-1 | Mus musculus (Mouse) | EV |
| O35343 | Kpna4 | Importin subunit alpha-3 | Mus musculus (Mouse) | SS |
| O35344 | Kpna3 | Importin subunit alpha-4 | Mus musculus (Mouse) | SS |
| O35345 | Kpna6 | Importin subunit alpha-7 | Mus musculus (Mouse) | PR |
| Q60960 | Kpna1 | Importin subunit alpha-5 | Mus musculus (Mouse) | SS |
| C0LLJ0 | Kpna7 | Importin subunit alpha-8 | Mus musculus (Mouse) | SS |
| C6K7I2 | KPNA7 | Importin subunit alpha-8 | Sus scrofa (Pig) | SS |
| Q56R16 | Kpna5 | Importin subunit alpha-6 | Rattus norvegicus (Rat) | SS |
| P83953 | Kpna1 | Importin subunit alpha-5 | Rattus norvegicus (Rat) | SS |
| Q71VM4 | Os01g0253300 | Importin subunit alpha-1a | Oryza sativa subsp. japonica (Rice) | SS |
| P91276 | ima-2 | Importin subunit alpha-2 | Caenorhabditis elegans | SS |
| Q19969 | ima-3 | Importin subunit alpha-3 | Caenorhabditis elegans | SS |
| O04294 | IMPA3 | Importin subunit alpha-3 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9M9X7 | IMPA7 | Importin subunit alpha-7 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FWY7 | IMPA6 | Importin subunit alpha-6 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O22478 | Importin subunit alpha | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) | SS | |
| Q503E9 | kpna5 | Importin subunit alpha-6 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSLRPSTKTE | IRRIRYKVSV | DAEEGRRRRE | DFLVEIRKSK | RNENLMKKRR | VKVLPPDYKL |
| 70 | 80 | 90 | 100 | 110 | 120 |
| ISNDPFESLL | EIANMITGVF | SDDPSLQLEY | TTRFRVVLSF | DRSPPTDNVI | KSGVVPRFVE |
| 130 | 140 | 150 | 160 | 170 | 180 |
| FLKKDDNPKL | QFEAAWALTN | IASGASEHTK | VVIDHGVVPL | FVQLLASPDD | DVREQAIWGL |
| 190 | 200 | 210 | 220 | 230 | 240 |
| GNVAGDSIQC | RDFVLNSGAF | IPLLHQLNNH | ATLSILRNAT | WTLSNFFRGK | PSPPFDLVKH |
| 250 | 260 | 270 | 280 | 290 | 300 |
| VLPVLKRLVY | SDDEQVLIDA | CWALSNLSDA | SNENIQSVIE | AGVVPRLVEL | LQHASPVVLV |
| 310 | 320 | 330 | 340 | 350 | 360 |
| PALRCIGNIV | SGNSQQTHCV | INCGVLPVLA | DLLTQNHMRG | IRREACWTIS | NITAGLEEQI |
| 370 | 380 | 390 | 400 | 410 | 420 |
| QSVIDANLIP | SLVNLAQHAE | FDIKKEAIWA | ISNASVGGSP | NQIKYLVEQN | CIKALCDILV |
| 430 | 440 | 450 | 460 | 470 | 480 |
| CPDLRIILVS | LGGLEMILIA | GEVDKNLRDV | NCYSQMIEDA | EGLEKIENLQ | HHGNNEIYEK |
| 490 | 500 | 510 | |||
| AVKILQTYGL | VEEDGRLVEE | EDEGGDGCSH | PEFQFDFSR |