Q9ES74

SS
Gene name
Nek7
Protein name
Serine/threonine-protein kinase Nek7
Names
Never in mitosis A-related kinase 7, NimA-related protein kinase 7
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:59125
EC number
2.7.11.34: Protein-serine/threonine kinases
Protein Class

Descriptions

Nek7 is a NIMA-related kinase essential for proper mitotic spindle assembly. A human homolog Nek7 (Q8TDX7) is subject to autoinhibition by a tyrosine motif, Tyr97, like Nek6 (Tyr108). This is because the Y97A mutation is more active than wild-type Nek7. A similar tyrosine motif is also found in mouse Nek7.

Autoinhibitory domains (AIDs)

97-102 (β4 sheet containing tyrosine motif) SS

Target domain

20-30 (N-terminal extension of kinase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

178-201 (Activation loop from InterPro)

Target domain

34-299 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q9ES74

Entry ID Method Resolution Chain Position Source
AF-Q9ES74-F1 Predicted AlphaFoldDB

No variants for Q9ES74

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9ES74

2 associated diseases with Q9ES74

[MIM: 613780]: Aortic aneurysm, familial thoracic 7 (AAT7)

A disease characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. It is primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. . Note=The disease is caused by variants affecting the gene represented in this entry.

[MIM: 249210]: Megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS)

A form of megacystis-microcolon-intestinal hypoperistalsis syndrome, a congenital visceral myopathy primarily affecting females, and characterized by loss of smooth muscle contraction in the bladder and intestine. Affected individuals present at birth with functional obstruction of intestine, microcolon, dilation of bladder, and secondary hydronephrosis. The majority of cases have a fatal outcome due to malnutrition and sepsis, followed by multiorgan failure. MMIHS inheritance is autosomal recessive. . Note=The disease is caused by variants affecting the gene represented in this entry.

Without disease ID
  • A disease characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. It is primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. . Note=The disease is caused by variants affecting the gene represented in this entry.
  • A form of megacystis-microcolon-intestinal hypoperistalsis syndrome, a congenital visceral myopathy primarily affecting females, and characterized by loss of smooth muscle contraction in the bladder and intestine. Affected individuals present at birth with functional obstruction of intestine, microcolon, dilation of bladder, and secondary hydronephrosis. The majority of cases have a fatal outcome due to malnutrition and sepsis, followed by multiorgan failure. MMIHS inheritance is autosomal recessive. . Note=The disease is caused by variants affecting the gene represented in this entry.

41 regional properties for Q9ES74

Type Name Position InterPro Accession
domain Protein kinase domain 1464 - 1719 IPR000719
domain Immunoglobulin subtype 2 45 - 113 IPR003598-1
domain Immunoglobulin subtype 2 173 - 240 IPR003598-2
domain Immunoglobulin subtype 2 426 - 494 IPR003598-3
domain Immunoglobulin subtype 2 526 - 590 IPR003598-4
domain Immunoglobulin subtype 2 635 - 702 IPR003598-5
domain Immunoglobulin subtype 2 733 - 801 IPR003598-6
domain Immunoglobulin subtype 2 1110 - 1177 IPR003598-7
domain Immunoglobulin subtype 2 1250 - 1317 IPR003598-8
domain Immunoglobulin subtype 2 1821 - 1889 IPR003598-9
domain Immunoglobulin subtype 39 - 124 IPR003599-1
domain Immunoglobulin subtype 167 - 251 IPR003599-2
domain Immunoglobulin subtype 420 - 505 IPR003599-3
domain Immunoglobulin subtype 520 - 601 IPR003599-4
domain Immunoglobulin subtype 629 - 713 IPR003599-5
domain Immunoglobulin subtype 727 - 812 IPR003599-6
domain Immunoglobulin subtype 1104 - 1188 IPR003599-7
domain Immunoglobulin subtype 1244 - 1328 IPR003599-8
domain Immunoglobulin subtype 1815 - 1900 IPR003599-9
domain Fibronectin type III 1331 - 1426 IPR003961
domain Immunoglobulin-like domain 33 - 122 IPR007110-1
domain Immunoglobulin-like domain 161 - 249 IPR007110-2
domain Immunoglobulin-like domain 414 - 503 IPR007110-3
domain Immunoglobulin-like domain 514 - 599 IPR007110-4
domain Immunoglobulin-like domain 620 - 711 IPR007110-5
domain Immunoglobulin-like domain 721 - 808 IPR007110-6
domain Immunoglobulin-like domain 1098 - 1186 IPR007110-7
domain Immunoglobulin-like domain 1238 - 1326 IPR007110-8
domain Immunoglobulin-like domain 1809 - 1898 IPR007110-9
active_site Serine/threonine-protein kinase, active site 1581 - 1593 IPR008271
domain Immunoglobulin I-set 33 - 123 IPR013098-1
domain Immunoglobulin I-set 161 - 250 IPR013098-2
domain Immunoglobulin I-set 414 - 504 IPR013098-3
domain Immunoglobulin I-set 514 - 600 IPR013098-4
domain Immunoglobulin I-set 624 - 712 IPR013098-5
domain Immunoglobulin I-set 722 - 809 IPR013098-6
domain Immunoglobulin I-set 1098 - 1187 IPR013098-7
domain Immunoglobulin I-set 1238 - 1327 IPR013098-8
domain Immunoglobulin I-set 1809 - 1899 IPR013098-9
domain Myosin Light Chain Kinase 1, Kinase domain 1461 - 1719 IPR015725
binding_site Protein kinase, ATP binding site 1470 - 1493 IPR017441

Functions

Description
EC Number 2.7.11.34 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
  • Cytoplasm
  • Cytoplasm, cytoskeleton, spindle pole
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Present at centrosome throughout the cell cycle (By similarity)
  • Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (By similarity)
  • Interaction with ANKS3 prevents its translocation to the nucleus (PubMed:26188091)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
metal ion binding Binding to a metal ion.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

6 GO annotations of biological process

Name Definition
cellular response to potassium ion Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a potassium ion stimulus.
positive regulation of NLRP3 inflammasome complex assembly Any process that activates or increases the frequency, rate or extent of NLRP3 inflammasome complex assembly.
positive regulation of telomerase activity Any process that activates or increases the frequency, rate or extent of telomerase activity, the catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
positive regulation of telomere capping Any process that activates or increases the frequency, rate or extent of telomere capping.
positive regulation of telomere maintenance via telomerase Any process that activates or increases the frequency, rate or extent of the addition of telomeric repeats by telomerase.
protein phosphorylation The process of introducing a phosphate group on to a protein.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9HC98 NEK6 Serine/threonine-protein kinase Nek6 Homo sapiens (Human) EV
Q8TDX7 NEK7 Serine/threonine-protein kinase Nek7 Homo sapiens (Human) EV
Q9ES70 Nek6 Serine/threonine-protein kinase Nek6 Mus musculus (Mouse) SS
A2BD05 NEK6 Serine/threonine-protein kinase Nek6 Sus scrofa (Pig) SS
P59895 Nek6 Serine/threonine-protein kinase Nek6 Rattus norvegicus (Rat) SS
D3ZBE5 Nek7 Serine/threonine-protein kinase Nek7 Rattus norvegicus (Rat) SS
G5EFM9 nekl-3 Serine/threonine-protein kinase nekl-3 Caenorhabditis elegans PR
10 20 30 40 50 60
MDEQSQGMQG PPVTQFQPQK ALRPDMGYNT LANFRIEKKI GRGQFSEVYR ASCLLDGVPV
70 80 90 100 110 120
ALKKVQIFDL MDAKARADCI KEIDLLKQLN HPNVIKYYAS FIEDNELNIV LELADAGDLS
130 140 150 160 170 180
RMIKHFKKQK RLIPERTVWK YFVQLCSALD HMHSRRVMHR DIKPANVFIT ATGVVKLGDL
190 200 210 220 230 240
GLGRFFSSKT TAAHSLVGTP YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK
250 260 270 280 290 300
MNLYSLCKKI EQCDYPPLPS DHYSEELRQL VNICINPDPE KRPDIAYVYD VAKRMHACTA
ST