Q9DG15
Gene name |
|
Protein name |
p95-APP1 |
Names |
|
Species |
Gallus gallus (Chicken) |
KEGG Pathway |
gga:417584 |
EC number |
|
Protein Class |
G PROTEIN-COUPLED RECEPTOR KINASE INTERACTING ARFGAP (PTHR46097) |
Descriptions
G-protein coupled receptor kinase-interacting protein (GIT, p95-APP1) proteins include an N-terminal Arf GTPase-activating protein domain (Arf-GAP domain), three ankyrin repeats, a Spa2-homology domain (SHD), a coiled-coil domain including a leucine zipper required for dimerization, and a paxillin-binding site (PBS). To autoinhibit the C-terminal domain, the N-terminal GAP domain (1-124) interacts with the C-terminal domain. In addition, the interaction between the ankyrin repeats and SHD domain is probably the main intramolecular interface to keep the inactive conformation (GAP-C terminal domain interaction) of p95-APP1. Therefore, the SHD domain is essential in mediating the intramolecular interactions between the C-terminal half of GIT1 and the N-terminal part including GAP domain and ankyrin repeats.
Autoinhibitory domains (AIDs)
Target domain |
257-347 (C-terminal SHD domain) |
Relief mechanism |
Partner binding, PTM |
Assay |
Deletion assay, Split protein assay |
Accessory elements
No accessory elements
References
Autoinhibited structure
Activated structure
1 structures for Q9DG15
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9DG15-F1 | Predicted | AlphaFoldDB |
No variants for Q9DG15
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q9DG15 | |||||
No associated diseases with Q9DG15
4 regional properties for Q9DG15
Functions
| Description | ||
|---|---|---|
| EC Number | ||
| Subcellular Localization |
|
|
| PANTHER Family | PTHR46097 | G PROTEIN-COUPLED RECEPTOR KINASE INTERACTING ARFGAP |
| PANTHER Subfamily | PTHR46097:SF1 | ARF GTPASE-ACTIVATING PROTEIN GIT1 |
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| ruffle membrane | The portion of the plasma membrane surrounding a ruffle. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| GTPase activator activity | Binds to and increases the activity of a GTPase, an enzyme that catalyzes the hydrolysis of GTP. |
| metal ion binding | Binding to a metal ion. |
2 GO annotations of biological process
| Name | Definition |
|---|---|
| regulation of cell communication | Any process that modulates the frequency, rate or extent of cell communication. Cell communication is the process that mediates interactions between a cell and its surroundings. Encompasses interactions such as signaling or attachment between one cell and another cell, between a cell and an extracellular matrix, or between a cell and any other aspect of its environment. |
| regulation of signaling | Any process that modulates the frequency, rate or extent of a signaling process. |
7 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q95RG8 | Git | ARF GTPase-activating protein Git | Drosophila melanogaster (Fruit fly) | SS |
| Q14161 | GIT2 | ARF GTPase-activating protein GIT2 | Homo sapiens (Human) | SS |
| Q9Y2X7 | GIT1 | ARF GTPase-activating protein GIT1 | Homo sapiens (Human) | SS |
| Q68FF6 | Git1 | ARF GTPase-activating protein GIT1 | Mus musculus (Mouse) | SS |
| Q9JLQ2 | Git2 | ARF GTPase-activating protein GIT2 | Mus musculus (Mouse) | SS |
| Q66H91 | Git2 | ARF GTPase-activating protein GIT2 | Rattus norvegicus (Rat) | SS |
| Q9Z272 | Git1 | ARF GTPase-activating protein GIT1 | Rattus norvegicus (Rat) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSRKAQRAEV | CADCSAADPG | WASINRGVLI | CDECCSVHRS | LGRHISIVKH | LRHSPWPATL |
| 70 | 80 | 90 | 100 | 110 | 120 |
| LQMVHTLASN | GANSIWEHSL | LDPAQVQSGR | RKANPQDKVH | PTKSEFIRAK | YQMLAFVHKL |
| 130 | 140 | 150 | 160 | 170 | 180 |
| PCRDDDGVTA | KDLSKQLHSS | VRTGNLETCL | RLLSLGAQAN | FFHPEKGTTP | LHVAAKAGQI |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LQAELLVVYG | ADPGAPDVNG | RTPIDYARQA | AQHELAERLV | ECQYELTDRL | AFYLCGRKPD |
| 250 | 260 | 270 | 280 | 290 | 300 |
| HKNGHYIIPQ | MADSLDLSEL | AKAAKKKLQA | LSNRLFEELA | MDVYDEVDRR | ENDAVWLTTQ |
| 310 | 320 | 330 | 340 | 350 | 360 |
| NHSTLVTERS | AVPFLPVNPE | YSATRNQGRQ | KLARFNAREF | ATLLIDILGE | AKRRQQGKSP |
| 370 | 380 | 390 | 400 | 410 | 420 |
| LSPTDALDYS | LRSQSDLDDQ | HDYDSVASDE | DTDQELLRNA | SRNNRARSMD | SSDLSDGPIT |
| 430 | 440 | 450 | 460 | 470 | 480 |
| LQEYLEVKKA | LAASEAKVQQ | LMKVNNSLSD | ELRRLQREIH | KLQSENTQIR | QQAVPPHPTP |
| 490 | 500 | 510 | 520 | 530 | 540 |
| APSERPEHGH | PPGTAPLHRR | DRQAFSMYEP | GSVLKPFGQP | VEELVTRLQP | FGGGMRKGPS |
| 550 | 560 | 570 | 580 | 590 | 600 |
| VSSMPFPPSS | PLLSCPPDGA | RHMSKLDRHG | SGTDSDYDNT | QTGEILLGVE | GKRFVELSKD |
| 610 | 620 | 630 | 640 | 650 | 660 |
| EDFPHELDPL | DGELDPGLPS | TEDVILKTEQ | VTKNIQELLR | AAQESKHDSF | VPCSEKIHSA |
| 670 | 680 | 690 | 700 | 710 | 720 |
| VTEMASLFPK | KPALETVRSS | LRLLNASAYR | LQSECRKTVP | PEPGAAVDYQ | LLTQQVIQCA |
| 730 | |||||
| YDIAKAAKQL | VTITTREKKQ |