Q9DBC7
SS
Gene name |
Prkar1a |
Protein name |
cAMP-dependent protein kinase type I-alpha regulatory subunit [Cleaved into: cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed] |
Names |
|
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:19084 |
EC number |
|
Protein Class |
|
Descriptions
PKA is a major intracellular receptor of cAMP and modulates diverse cellular signalings. The autoinhibitory sequence of the regulatory subunit may interact with the catalytic kinase domain and keeping the catalytic subunit inactive by blocking the active site in the absence of cAMP.
Autoinhibitory domains (AIDs)
Target domain |
137-253 (Cyclic nucleotide-binding domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
No accessory elements
References
- Turnham RE et al. (2016) "Protein kinase A catalytic subunit isoform PRKACA; History, function and physiology", Gene, 577, 101-8
- Scott JD (1991) "Cyclic nucleotide-dependent protein kinases", Pharmacology & therapeutics, 50, 123-45
- Diskar M et al. (2007) "Molecular basis for isoform-specific autoregulation of protein kinase A", Cellular signalling, 19, 2024-34
- Diller TC et al. (2000) "Type II beta regulatory subunit of cAMP-dependent protein kinase: purification strategies to optimize crystallization", Protein expression and purification, 20, 357-64
Autoinhibited structure
Activated structure
1 structures for Q9DBC7
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9DBC7-F1 | Predicted | AlphaFoldDB |
10 variants for Q9DBC7
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs253413028 | 41 | T>A | No | EVA | |
| rs3389222439 | 60 | E>V | No | EVA | |
| rs3389148477 | 120 | K>* | No | EVA | |
| rs3389211005 | 124 | T>K | No | EVA | |
| rs3389210295 | 127 | A>D | No | EVA | |
| rs3389148437 | 134 | K>R | No | EVA | |
| rs3389183895 | 144 | N>S | No | EVA | |
| rs3389183839 | 250 | L>I | No | EVA | |
| rs3389213396 | 251 | S>T | No | EVA | |
| rs3402902640 | 344 | P>R | No | EVA |
No associated diseases with Q9DBC7
9 regional properties for Q9DBC7
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 1120 - 1396 | IPR000719 |
| domain | MAM domain | 268 - 431 | IPR000998-1 |
| domain | MAM domain | 482 - 640 | IPR000998-2 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 1120 - 1386 | IPR001245 |
| conserved_site | Tyrosine-protein kinase, receptor class II, conserved site | 1280 - 1288 | IPR002011 |
| repeat | Low-density lipoprotein (LDL) receptor class A repeat | 441 - 477 | IPR002172 |
| active_site | Tyrosine-protein kinase, active site | 1249 - 1261 | IPR008266 |
| binding_site | Protein kinase, ATP binding site | 1126 - 1154 | IPR017441 |
| domain | Tyrosine-protein kinase, catalytic domain | 1120 - 1387 | IPR020635 |
13 GO annotations of cellular component
| Name | Definition |
|---|---|
| axoneme | The bundle of microtubules and associated proteins that forms the core of cilia (also called flagella) in eukaryotic cells and is responsible for their movements. |
| cAMP-dependent protein kinase complex | An enzyme complex, composed of regulatory and catalytic subunits, that catalyzes protein phosphorylation. Inactive forms of the enzyme have two regulatory chains and two catalytic chains; activation by cAMP produces two active catalytic monomers and a regulatory dimer. |
| centrosome | A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| glutamatergic synapse | A synapse that uses glutamate as a neurotransmitter. |
| immunological synapse | An area of close contact between a lymphocyte (T-, B-, or natural killer cell) and a target cell formed through the clustering of particular signaling and adhesion molecules and their associated membrane rafts on both the lymphocyte and the target cell and facilitating activation of the lymphocyte, transfer of membrane from the target cell to the lymphocyte, and in some situations killing of the target cell through release of secretory granules and/or death-pathway ligand-receptor interaction. |
| multivesicular body | A type of endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm. |
| neuromuscular junction | The junction between the axon of a motor neuron and a muscle fiber. In response to the arrival of action potentials, the presynaptic button releases molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane of the muscle fiber, leading to a change in post-synaptic potential. |
| nucleotide-activated protein kinase complex | A protein complex that possesses nucleotide-dependent protein kinase activity. The nucleotide can be AMP (in S. pombe and human) or ADP (in S. cerevisiae). |
| plasma membrane raft | A membrane raft that is part of the plasma membrane. |
| sperm connecting piece | The segment of the sperm flagellum that attaches to the implantation fossa of the nucleus in the sperm head; from the remnant of the centriole at this point, the axoneme extends throughout the length of the flagellum. |
| synapse | The junction between an axon of one neuron and a dendrite of another neuron, a muscle fiber or a glial cell. As the axon approaches the synapse it enlarges into a specialized structure, the presynaptic terminal bouton, which contains mitochondria and synaptic vesicles. At the tip of the terminal bouton is the presynaptic membrane; facing it, and separated from it by a minute cleft (the synaptic cleft) is a specialized area of membrane on the receiving cell, known as the postsynaptic membrane. In response to the arrival of nerve impulses, the presynaptic terminal bouton secretes molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| cAMP binding | Binding to cAMP, the nucleotide cyclic AMP (adenosine 3',5'-cyclophosphate). |
| cAMP-dependent protein kinase inhibitor activity | Binds to and stops, prevents or reduces the activity of a cAMP-dependent protein kinase. |
| cAMP-dependent protein kinase regulator activity | Modulation of the activity of the enzyme cAMP-dependent protein kinase. |
| protein domain specific binding | Binding to a specific domain of a protein. |
| protein kinase A catalytic subunit binding | Binding to one or both of the catalytic subunits of protein kinase A. |
| ubiquitin protein ligase binding | Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins. |
11 GO annotations of biological process
| Name | Definition |
|---|---|
| adenylate cyclase-activating G protein-coupled receptor signaling pathway | A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation of adenylyl cyclase activity and a subsequent increase in the intracellular concentration of cyclic AMP (cAMP). |
| animal organ morphogenesis | Morphogenesis of an animal organ. An organ is defined as a tissue or set of tissues that work together to perform a specific function or functions. Morphogenesis is the process in which anatomical structures are generated and organized. Organs are commonly observed as visibly distinct structures, but may also exist as loosely associated clusters of cells that work together to perform a specific function or functions. |
| cardiac muscle cell proliferation | The expansion of a cardiac muscle cell population by cell division. |
| cellular response to glucagon stimulus | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucagon stimulus. |
| heart development | The process whose specific outcome is the progression of the heart over time, from its formation to the mature structure. The heart is a hollow, muscular organ, which, by contracting rhythmically, keeps up the circulation of the blood. |
| mesenchymal cell proliferation | The multiplication or reproduction of cells, resulting in the expansion of a mesenchymal cell population. A mesenchymal cell is a cell that normally gives rise to other cells that are organized as three-dimensional masses, rather than sheets. |
| mesoderm formation | The process that gives rise to the mesoderm. This process pertains to the initial formation of the structure from unspecified parts. |
| negative regulation of activated T cell proliferation | Any process that stops, prevents or reduces the rate or extent of activated T cell proliferation. |
| negative regulation of gene expression | Any process that decreases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA). |
| positive regulation of insulin secretion | Any process that activates or increases the frequency, rate or extent of the regulated release of insulin. |
| sarcomere organization | The myofibril assembly process that results in the organization of muscle actomyosin into sarcomeres. The sarcomere is the repeating unit of a myofibril in a muscle cell, composed of an array of overlapping thick and thin filaments between two adjacent Z discs. |
22 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P31322 | PRKAR2B | cAMP-dependent protein kinase type II-beta regulatory subunit | Bos taurus (Bovine) | EV |
| P00515 | PRKAR2A | cAMP-dependent protein kinase type II-alpha regulatory subunit | Bos taurus (Bovine) | EV |
| P00514 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Bos taurus (Bovine) | EV |
| Q5ZM91 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Gallus gallus (Chicken) | SS |
| P81900 | Pka-R2 | cAMP-dependent protein kinase type II regulatory subunit | Drosophila melanogaster (Fruit fly) | PR |
| P16905 | Pka-R1 | cAMP-dependent protein kinase type I regulatory subunit | Drosophila melanogaster (Fruit fly) | PR |
| Q96M20 | CNBD2 | Cyclic nucleotide-binding domain-containing protein 2 | Homo sapiens (Human) | PR |
| P13861 | PRKAR2A | cAMP-dependent protein kinase type II-alpha regulatory subunit | Homo sapiens (Human) | PR |
| P31321 | PRKAR1B | cAMP-dependent protein kinase type I-beta regulatory subunit | Homo sapiens (Human) | EV |
| P31323 | PRKAR2B | cAMP-dependent protein kinase type II-beta regulatory subunit | Homo sapiens (Human) | PR |
| P10644 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Homo sapiens (Human) | EV |
| P12367 | Prkar2a | cAMP-dependent protein kinase type II-alpha regulatory subunit | Mus musculus (Mouse) | PR |
| Q9D5U8 | Cnbd2 | Cyclic nucleotide-binding domain-containing protein 2 | Mus musculus (Mouse) | PR |
| P31324 | Prkar2b | cAMP-dependent protein kinase type II-beta regulatory subunit | Mus musculus (Mouse) | PR |
| P12849 | Prkar1b | cAMP-dependent protein kinase type I-beta regulatory subunit | Mus musculus (Mouse) | PR |
| P05207 | PRKAR2A | cAMP-dependent protein kinase type II-alpha regulatory subunit | Sus scrofa (Pig) | SS |
| P07802 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Sus scrofa (Pig) | SS |
| P81377 | Prkar1b | cAMP-dependent protein kinase type I-beta regulatory subunit | Rattus norvegicus (Rat) | PR |
| P12368 | Prkar2a | cAMP-dependent protein kinase type II-alpha regulatory subunit | Rattus norvegicus (Rat) | PR |
| P12369 | Prkar2b | cAMP-dependent protein kinase type II-beta regulatory subunit | Rattus norvegicus (Rat) | PR |
| P09456 | Prkar1a | cAMP-dependent protein kinase type I-alpha regulatory subunit | Rattus norvegicus (Rat) | SS |
| P30625 | kin-2 | cAMP-dependent protein kinase regulatory subunit | Caenorhabditis elegans | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MASGSMATSE | EERSLRECEL | YVQKHNIQAL | LKDSIVQLCT | TRPERPMAFL | REYFERLEKE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| EARQIQCLQK | TGIRTDSRED | EISPPPPNPV | VKGRRRRGAI | SAEVYTEEDA | ASYVRKVIPK |
| 130 | 140 | 150 | 160 | 170 | 180 |
| DYKTMAALAK | AIEKNVLFSH | LDDNERSDIF | DAMFPVSFIA | GETVIQQGDE | GDNFYVIDQG |
| 190 | 200 | 210 | 220 | 230 | 240 |
| EMDVYVNNEW | ATSVGEGGSF | GELALIYGTP | RAATVKAKTN | VKLWGIDRDS | YRRILMGSTL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| RKRKMYEEFL | SKVSILESLD | KWERLTVADA | LEPVQFEDGQ | KIVVQGEPGD | EFFIILEGTA |
| 310 | 320 | 330 | 340 | 350 | 360 |
| AVLQRRSENE | EFVEVGRLGP | SDYFGEIALL | MNRPRAATVV | ARGPLKCVKL | DRPRFERVLG |
| 370 | 380 | ||||
| PCSDILKRNI | QQYNSFVSLS | V |