Q9D8B3
SS
Gene name |
Chmp4b |
Protein name |
Charged multivesicular body protein 4b |
Names |
Chromatin-modifying protein 4b, CHMP4b |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:75608 |
EC number |
|
Protein Class |
|
Descriptions
Charged multivesicular body protein 4b (Chmp4b) is a core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. In a study with human CHMP2A, removing 42 amino acids from the C-terminal of the protein unmasks a common ability to associate with endosomal membranes and assemble into large polymeric complexes, and the C-terminal sequences are also important for interaction of ESCRT-III protein with VPS4. Thus, ESCRT-III proteins cycle between a closed state and an activated open state is under control of sequences at the C-terminus and associated factors.
Autoinhibitory domains (AIDs)
Target domain |
1-144 (N-terminal α1-α4 domains) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q9D8B3
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9D8B3-F1 | Predicted | AlphaFoldDB |
1 variants for Q9D8B3
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs13472651 | 103 | T>M | No | Ensembl |
No associated diseases with Q9D8B3
No regional properties for Q9D8B3
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| No domain, repeats, and functional sites for Q9D8B3 | |||
Functions
20 GO annotations of cellular component
| Name | Definition |
|---|---|
| amphisome membrane | Any membrane that is part of an amphisome. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytoplasmic side of plasma membrane | The leaflet the plasma membrane that faces the cytoplasm and any proteins embedded or anchored in it or attached to its surface. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| endosome | A vacuole to which materials ingested by endocytosis are delivered. |
| ESCRT III complex | A complex with membrane scission activity that plays a major role in many processes where membranes are remodelled - including endosomal transport (vesicle budding), nuclear envelope organisation (membrane closure, mitotic bridge cleavage), and cytokinesis (abscission). |
| glutamatergic synapse | A synapse that uses glutamate as a neurotransmitter. |
| kinetochore | A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules. |
| kinetochore microtubule | Any of the spindle microtubules that attach to the kinetochores of chromosomes by their plus ends, and maneuver the chromosomes during mitotic or meiotic chromosome segregation. |
| lysosomal membrane | The lipid bilayer surrounding the lysosome and separating its contents from the cell cytoplasm. |
| membrane | A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it. |
| membrane coat | Any of several different proteinaceous coats that can associate with membranes. Membrane coats include those formed by clathrin plus an adaptor complex, the COPI and COPII complexes, and possibly others. They are found associated with membranes on many vesicles as well as other membrane features such as pits and perhaps tubules. |
| midbody | A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis. |
| multivesicular body | A type of endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm. |
| multivesicular body membrane | The lipid bilayer surrounding a multivesicular body. |
| nuclear envelope | The double lipid bilayer enclosing the nucleus and separating its contents from the rest of the cytoplasm; includes the intermembrane space, a gap of width 20-40 nm (also called the perinuclear space). |
| nuclear pore | A protein complex providing a discrete opening in the nuclear envelope of a eukaryotic cell, where the inner and outer nuclear membranes are joined. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| postsynaptic density | An electron dense network of proteins within and adjacent to the postsynaptic membrane of an asymmetric, neuron-neuron synapse. Its major components include neurotransmitter receptors and the proteins that spatially and functionally organize them such as anchoring and scaffolding molecules, signaling enzymes and cytoskeletal components. |
| vesicle | Any small, fluid-filled, spherical organelle enclosed by membrane. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| identical protein binding | Binding to an identical protein or proteins. |
| protein homodimerization activity | Binding to an identical protein to form a homodimer. |
28 GO annotations of biological process
| Name | Definition |
|---|---|
| autophagosome maturation | Removal of PI3P and Atg8/LC3 after the closure of the phagophore and before the fusion with the endosome/lysosome (e.g. mammals and insects) or vacuole (yeast), and that very likely destabilizes other Atg proteins and thus enables their efficient dissociation and recycling. |
| autophagy | The cellular catabolic process in which cells digest parts of their own cytoplasm; allows for both recycling of macromolecular constituents under conditions of cellular stress and remodeling the intracellular structure for cell differentiation. |
| exit from mitosis | The cell cycle transition where a cell leaves M phase and enters a new G1 phase. M phase is the part of the mitotic cell cycle during which mitosis and cytokinesis take place. |
| late endosome to lysosome transport | The directed movement of substances from late endosome to lysosome. |
| late endosome to vacuole transport via multivesicular body sorting pathway | The directed movement of substances from endosomes to vacuoles by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the vacuole. |
| maintenance of lens transparency | A homeostatic process in which the lens is maintained in a highly refractive, transparent state to allow for optimal focusing of light on the retina. |
| membrane fission | A process that is carried out at the cellular level which results in the separation of a single continuous membrane into two membranes. |
| midbody abscission | The process by which the midbody, the cytoplasmic bridge that connects the two prospective daughter cells, is severed at the end of mitotic cytokinesis, resulting in two separate daughter cells. |
| mitotic cytokinesis | A cell cycle process that results in the division of the cytoplasm of a cell after mitosis, resulting in the separation of the original cell into two daughter cells. |
| mitotic metaphase plate congression | The cell cycle process in which chromosomes are aligned at the metaphase plate, a plane halfway between the poles of the mitotic spindle, during mitosis. |
| negative regulation of autophagosome assembly | Any process that stops, prevents or reduces the frequency, rate or extent of autophagosome assembly. |
| negative regulation of cell death | Any process that decreases the rate or frequency of cell death. Cell death is the specific activation or halting of processes within a cell so that its vital functions markedly cease, rather than simply deteriorating gradually over time, which culminates in cell death. |
| negative regulation of neuron death | Any process that stops, prevents or reduces the frequency, rate or extent of neuron death. |
| nuclear membrane reassembly | The reformation of the nuclear membranes following their breakdown in the context of a normal process. |
| nucleus organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the nucleus. |
| plasma membrane repair | The resealing of a cell plasma membrane after cellular wounding due to, for instance, mechanical stress. |
| posttranslational protein targeting to endoplasmic reticulum membrane | The targeting of proteins to a membrane that occurs after their translation. Some secretory proteins exhibit posttranslational transport into the endoplasmic reticulum (ER) lumen: they are synthesized in their entirety on free cytosolic ribosomes and then released into the cytosol, where they are bound by chaperones which keep them in an unfolded state, and subsequently are translocated across the ER membrane. |
| protein polymerization | The process of creating protein polymers, compounds composed of a large number of component monomers; polymeric proteins may be made up of different or identical monomers. Polymerization occurs by the addition of extra monomers to an existing poly- or oligomeric protein. |
| regulation of autophagy | Any process that modulates the frequency, rate or extent of autophagy. Autophagy is the process in which cells digest parts of their own cytoplasm. |
| regulation of centrosome duplication | Any process that modulates the frequency, rate or extent of centrosome duplication. Centrosome duplication is the replication of a centrosome, a structure comprised of a pair of centrioles and peri-centriolar material from which a microtubule spindle apparatus is organized. |
| regulation of mitotic spindle assembly | Any process that modulates the frequency, rate or extent of mitotic spindle assembly. |
| regulation of postsynapse organization | Any process that modulates the physical form of a postsynapse. |
| ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | The chemical reactions and pathways resulting in the breakdown of a protein or peptide covalently tagged with ubiquitin, via the multivesicular body (MVB) sorting pathway; ubiquitin-tagged proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation. |
| ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway | The chemical reactions and pathways resulting in the breakdown of a protein or peptide, via the multivesicular body (MVB) sorting pathway; proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation. This process is independent of ubiquitination. |
| vesicle budding from membrane | The evagination of a membrane, resulting in formation of a vesicle. |
| viral budding | A viral process by which enveloped viruses acquire a host-derived membrane enriched in viral proteins to form their external envelope. The process starts when nucleocapsids, assembled or in the process of being built, induce formation of a membrane curvature in the host plasma or organelle membrane and wrap up in the forming bud. The process ends when the bud is eventually pinched off by membrane scission to release the enveloped particle into the lumenal or extracellular space. |
| viral budding from plasma membrane | A viral budding that starts with formation of a membrane curvature in the host plasma membrane. |
| viral budding via host ESCRT complex | Viral budding which uses a host ESCRT protein complex, or complexes, to mediate the budding process. |
3 homologous proteins in AiPD
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSVFGKLFGA | GGGKAGKGGP | TPQEAIQRLR | DTEEMLSKKQ | EFLEKKIEQE | LTAAKKHGTK |
| 70 | 80 | 90 | 100 | 110 | 120 |
| NKRAALQALK | RKKRYEKQLA | QIDGTLSTIE | FQREALENAN | TNTEVLKNMG | YAAKAMKAAH |
| 130 | 140 | 150 | 160 | 170 | 180 |
| DNMDIDKVDE | LMQDIADQQE | LAEEISTAIS | KPVGFGEEFD | EDELMAELEE | LEQEELDKNL |
| 190 | 200 | 210 | 220 | ||
| LEISGPETVP | LPNVPSVALP | SKPAKKKEEE | DDDMKELENW | AGSM |