Q9D7F7

SS
Gene name
Chmp4c
Protein name
Charged multivesicular body protein 4c
Names
Chromatin-modifying protein 4c, CHMP4c
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:66371
EC number
Protein Class

Descriptions

Charged multivesicular body protein 4c (Chmp4c) is a core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. In a study with human CHMP2A, removing 42 amino acids from the C-terminal of the protein unmasks a common ability to associate with endosomal membranes and assemble into large polymeric complexes, and the C-terminal sequences are also important for interaction of ESCRT-III protein with VPS4. Thus, ESCRT-III proteins cycle between a closed state and an activated open state is under control of sequences at the C-terminus and associated factors.

Autoinhibitory domains (AIDs)

187-232 (C-terminal autoinhibitory region) SS

Target domain

1-144 (N-terminal α1-α4 domains)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9D7F7

Entry ID Method Resolution Chain Position Source
AF-Q9D7F7-F1 Predicted AlphaFoldDB

5 variants for Q9D7F7

Variant ID(s) Position Change Description Diseaes Association Provenance
rs1134848785 18 A>V No Ensembl
rs29707553 21 S>R No Ensembl
rs29709942 121 D>E No Ensembl
rs244653197 122 N>S No Ensembl
rs253408595 219 E>D No Ensembl

No associated diseases with Q9D7F7

No regional properties for Q9D7F7

Type Name Position InterPro Accession
No domain, repeats, and functional sites for Q9D7F7

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytosol
  • Late endosome membrane ; Peripheral membrane protein
  • Midbody, Midbody ring
  • Localizes to the midbody during late cytokinesis
  • During its recruitment, localizes initially to the midbody arms, before being directed to the central region, the midbody ring, also called Flemming body
  • Phosphorylation at Ser-210 by AURKB triggers localization to midbody ring
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

12 GO annotations of cellular component

Name Definition
amphisome membrane Any membrane that is part of an amphisome.
cytoplasmic side of plasma membrane The leaflet the plasma membrane that faces the cytoplasm and any proteins embedded or anchored in it or attached to its surface.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
ESCRT III complex A complex with membrane scission activity that plays a major role in many processes where membranes are remodelled - including endosomal transport (vesicle budding), nuclear envelope organisation (membrane closure, mitotic bridge cleavage), and cytokinesis (abscission).
Flemming body A cell part that is the central region of the midbody characterized by a gap in alpha-tubulin staining. It is a dense structure of antiparallel microtubules from the central spindle in the middle of the intercellular bridge.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
kinetochore microtubule Any of the spindle microtubules that attach to the kinetochores of chromosomes by their plus ends, and maneuver the chromosomes during mitotic or meiotic chromosome segregation.
lysosomal membrane The lipid bilayer surrounding the lysosome and separating its contents from the cell cytoplasm.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
multivesicular body A type of endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.
multivesicular body membrane The lipid bilayer surrounding a multivesicular body.
nuclear pore A protein complex providing a discrete opening in the nuclear envelope of a eukaryotic cell, where the inner and outer nuclear membranes are joined.

1 GO annotations of molecular function

Name Definition
protein homodimerization activity Binding to an identical protein to form a homodimer.

20 GO annotations of biological process

Name Definition
abscission The controlled shedding of a body part.
autophagosome maturation Removal of PI3P and Atg8/LC3 after the closure of the phagophore and before the fusion with the endosome/lysosome (e.g. mammals and insects) or vacuole (yeast), and that very likely destabilizes other Atg proteins and thus enables their efficient dissociation and recycling.
late endosome to lysosome transport The directed movement of substances from late endosome to lysosome.
late endosome to vacuole transport via multivesicular body sorting pathway The directed movement of substances from endosomes to vacuoles by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the vacuole.
midbody abscission The process by which the midbody, the cytoplasmic bridge that connects the two prospective daughter cells, is severed at the end of mitotic cytokinesis, resulting in two separate daughter cells.
mitotic cytokinesis checkpoint signaling A signaling process that contributes to a mitotic cell cycle checkpoint that detects a defect in cytokinesis and prevents further rounds of nuclear division until cytokinesis is completed.
mitotic metaphase plate congression The cell cycle process in which chromosomes are aligned at the metaphase plate, a plane halfway between the poles of the mitotic spindle, during mitosis.
negative regulation of cell death Any process that decreases the rate or frequency of cell death. Cell death is the specific activation or halting of processes within a cell so that its vital functions markedly cease, rather than simply deteriorating gradually over time, which culminates in cell death.
negative regulation of cytokinesis Any process that stops, prevents, or reduces the frequency, rate or extent of the division of the cytoplasm of a cell, and its separation into two daughter cells.
nuclear membrane reassembly The reformation of the nuclear membranes following their breakdown in the context of a normal process.
nucleus organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the nucleus.
plasma membrane repair The resealing of a cell plasma membrane after cellular wounding due to, for instance, mechanical stress.
protein transport The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of centrosome duplication Any process that modulates the frequency, rate or extent of centrosome duplication. Centrosome duplication is the replication of a centrosome, a structure comprised of a pair of centrioles and peri-centriolar material from which a microtubule spindle apparatus is organized.
regulation of mitotic spindle assembly Any process that modulates the frequency, rate or extent of mitotic spindle assembly.
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway The chemical reactions and pathways resulting in the breakdown of a protein or peptide covalently tagged with ubiquitin, via the multivesicular body (MVB) sorting pathway; ubiquitin-tagged proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation.
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway The chemical reactions and pathways resulting in the breakdown of a protein or peptide, via the multivesicular body (MVB) sorting pathway; proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation. This process is independent of ubiquitination.
vesicle budding from membrane The evagination of a membrane, resulting in formation of a vesicle.
viral budding from plasma membrane A viral budding that starts with formation of a membrane curvature in the host plasma membrane.
viral budding via host ESCRT complex Viral budding which uses a host ESCRT protein complex, or complexes, to mediate the budding process.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9BY43 CHMP4A Charged multivesicular body protein 4a Homo sapiens (Human) SS
Q9D8B3 Chmp4b Charged multivesicular body protein 4b Mus musculus (Mouse) SS
Q569C1 Chmp4c Charged multivesicular body protein 4c Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MSKLGKFFKG TRSSRARAAP SAQEALARLR ETEEMLAKKQ EYLENRIQRE LALAKKHGSQ
70 80 90 100 110 120
NKRAALQALK RKKRFEKQLT QVDGTLSTIE FQREALENSH TNTEVLRNMG FAAKAMKAVH
130 140 150 160 170 180
DNMDLNKIDD LMQDITEQQD IAQEISEAFS QRVQFADGFD EAELLAELEE LEQEELNKKM
190 200 210 220 230
TSLELPNVPS SSLPAQPSRK ASMPSSVHRS RAASSRRAEE DDDFKQLAAW AT