Q9CWR0

SS
Gene name
Arhgef25 (D10Ertd610e, Geft)
Protein name
Rho guanine nucleotide exchange factor 25
Names
Guanine nucleotide exchange factor GEFT, Rac/Cdc42/Rho exchange factor GEFT, RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT, p63RhoGEF
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:52666
EC number
Protein Class

Descriptions

Dbl-family GEFs are the major class of exchange factors for Rho GTPases. The Dbl-family GEF p63RhoGEF, called ARHGEF25, is a mediator of Gαq/11 signaling to RhoA based on its ability to synergize with Gαq/11 resulting in enhanced RhoA signaling. Activated Gαq directly engages and stimulates p63RhoGEF. In human ARHGEF25, the protein is autoinhibited by the Dbl homology (DH)-associated pleckstrin homology (PH) domain. Activated Gαq relieves this autoinhibition by interacting with a highly conserved C-terminal extension of the PH domain.

Autoinhibitory domains (AIDs)

387-505 (PH domain) SS

Target domain

199-375 (DH domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9CWR0

Entry ID Method Resolution Chain Position Source
AF-Q9CWR0-F1 Predicted AlphaFoldDB

No variants for Q9CWR0

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q9CWR0

No associated diseases with Q9CWR0

2 regional properties for Q9CWR0

Type Name Position InterPro Accession
domain Protein kinase domain 208 - 474 IPR000719
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 210 - 469 IPR001245

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, myofibril, sarcomere
  • Cell membrane
  • Cytoplasm, myofibril
  • Highly colocalizes with actin regions
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
extrinsic component of membrane The component of a membrane consisting of gene products and protein complexes that are loosely bound to one of its surfaces, but not integrated into the hydrophobic region.
myofibril The contractile element of skeletal and cardiac muscle; a long, highly organized bundle of actin, myosin, and other proteins that contracts by a sliding filament mechanism.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
sarcomere The repeating unit of a myofibril in a muscle cell, composed of an array of overlapping thick and thin filaments between two adjacent Z discs.

1 GO annotations of molecular function

Name Definition
guanyl-nucleotide exchange factor activity Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions.

2 GO annotations of biological process

Name Definition
axon guidance The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues.
regulation of Rho protein signal transduction Any process that modulates the frequency, rate or extent of Rho protein signal transduction.

5 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q86VW2 ARHGEF25 Rho guanine nucleotide exchange factor 25 Homo sapiens (Human) EV
Q0KL02 Trio Triple functional domain protein Mus musculus (Mouse) SS
A2CG49 Kalrn Kalirin Mus musculus (Mouse) SS
Q64096 Mcf2l Guanine nucleotide exchange factor DBS Mus musculus (Mouse) SS
Q6P720 Arhgef25 Rho guanine nucleotide exchange factor 25 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MKPPDRPTPG RTDRILGVMG GMLRACAVPG QEGPPERDPL GPGSTKTESD CIEEDQTGQR
70 80 90 100 110 120
EPEVLAWAPQ PESYSIAVSE GSMSASAVSG LAALSGPSSG LSSDPCSPIP PGPVTGLRRW
130 140 150 160 170 180
LDHSKHCLSV ETEAESGQTG QCENWTLGPT LTTGQELPEL TLLTTLLEGP GDKAQPAEEE
190 200 210 220 230 240
TLSQAPKNEE EQKKMALERS MFVLGELVET ERTYEDDLGQ IVEGYMATMA TQGVPESLRG
250 260 270 280 290 300
RDRIVFGNIQ QIYEWHRDYF LQELQQCLKD PDWLAQLFIK HERRLHMYVV YCQNKPKSEH
310 320 330 340 350 360
VLSEFGDSYF EELRQQLGHR LQLNDLLIKP VQRIMKYQLL LKDFLKYYRR AGKDTEELEQ
370 380 390 400 410 420
AVEVMCFVPK RCNDMMSLGR LRGFEGKLTA QGKLLGQDTF LVTEPEAGGL LSSRGRERRV
430 440 450 460 470 480
FLFEQIIIFS EALGGGGRGG AQPGYVYKNS IKVSCLGLEG NLQGNPCRFA LTSRGPEGGI
490 500 510 520 530 540
QRYVLQASDP AVSQAWIKQV AQILESQRDF LNALQSPIEY QRRESQTNSL GRPGGPWVGS
550 560 570 580 590 600
PGRMRPGDLA QASMHTPING SLPSLLLLPR GEVSRVLLPL DTQALSDTPQ TPHDSPALPT
610
VNTPPCQARL AKLDEDEL