Q9CQ10
SS
Gene name |
Chmp3 (Vps24) |
Protein name |
Charged multivesicular body protein 3 |
Names |
Chromatin-modifying protein 3 , Vacuolar protein sorting-associated protein 24 |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:66700 |
EC number |
|
Protein Class |
|
Descriptions
CHMP3 is a core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The autoinhibition of CHMP3 is regulated through the intramolecular interactions between N-terminal basic domain and C-terminal acidic domain. The binding of the endosome-associated ubiquitin isopeptidase (AMSH) to the acidic half relieves the autoinhibition of CHMP3. CHMP3 can be induced to block HIV-1 release (Anti-HIV-1 budding activity) by coexpressing AMSH or by truncating the acidic domain.
Autoinhibitory domains (AIDs)
Target domain |
1-150 (N-terminal domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Zamborlini A et al. (2006) "Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding", Proceedings of the National Academy of Sciences of the United States of America, 103, 19140-5
- Bajorek M et al. (2009) "Structural basis for ESCRT-III protein autoinhibition", Nature structural & molecular biology, 16, 754-62
Autoinhibited structure
Activated structure
1 structures for Q9CQ10
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q9CQ10-F1 | Predicted | AlphaFoldDB |
13 variants for Q9CQ10
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3388833336 | 45 | K>R | No | EVA | |
| rs3388813792 | 63 | L>M | No | EVA | |
| rs3388831049 | 83 | H>Q | No | EVA | |
| rs3388837307 | 92 | K>R | No | EVA | |
| rs3388813638 | 136 | K>T | No | EVA | |
| rs3388842330 | 137 | A>V | No | EVA | |
| rs3388833340 | 146 | D>V | No | EVA | |
| rs3388839847 | 154 | Q>K | No | EVA | |
| rs3388831137 | 166 | D>H | No | EVA | |
| rs258181404 | 194 | A>T | No | EVA | |
| rs3544445795 | 199 | A>T | No | EVA | |
| rs3544441745 | 218 | R>Q | No | EVA | |
| rs3544422222 | 225 | S>Q | No | EVA |
No associated diseases with Q9CQ10
14 GO annotations of cellular component
| Name | Definition |
|---|---|
| amphisome membrane | Any membrane that is part of an amphisome. |
| autophagosome membrane | The lipid bilayer surrounding an autophagosome, a double-membrane-bounded vesicle in which endogenous cellular material is sequestered. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| early endosome | A membrane-bounded organelle that receives incoming material from primary endocytic vesicles that have been generated by clathrin-dependent and clathrin-independent endocytosis; vesicles fuse with the early endosome to deliver cargo for sorting into recycling or degradation pathways. |
| ESCRT III complex | A complex with membrane scission activity that plays a major role in many processes where membranes are remodelled - including endosomal transport (vesicle budding), nuclear envelope organisation (membrane closure, mitotic bridge cleavage), and cytokinesis (abscission). |
| kinetochore | A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules. |
| kinetochore microtubule | Any of the spindle microtubules that attach to the kinetochores of chromosomes by their plus ends, and maneuver the chromosomes during mitotic or meiotic chromosome segregation. |
| late endosome | A prelysosomal endocytic organelle differentiated from early endosomes by lower lumenal pH and different protein composition. Late endosomes are more spherical than early endosomes and are mostly juxtanuclear, being concentrated near the microtubule organizing center. |
| lysosomal membrane | The lipid bilayer surrounding the lysosome and separating its contents from the cell cytoplasm. |
| midbody | A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis. |
| multivesicular body | A type of endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm. |
| multivesicular body membrane | The lipid bilayer surrounding a multivesicular body. |
| nuclear pore | A protein complex providing a discrete opening in the nuclear envelope of a eukaryotic cell, where the inner and outer nuclear membranes are joined. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| phosphatidylcholine binding | Binding to a phosphatidylcholine, a glycophospholipid in which a phosphatidyl group is esterified to the hydroxyl group of choline. |
| phosphatidylinositol-4,5-bisphosphate binding | Binding to phosphatidylinositol-4,5-bisphosphate, a derivative of phosphatidylinositol in which the inositol ring is phosphorylated at the 4' and 5' positions. |
| ubiquitin-specific protease binding | Binding to a ubiquitin-specific protease. |
27 GO annotations of biological process
| Name | Definition |
|---|---|
| autophagosome maturation | Removal of PI3P and Atg8/LC3 after the closure of the phagophore and before the fusion with the endosome/lysosome (e.g. mammals and insects) or vacuole (yeast), and that very likely destabilizes other Atg proteins and thus enables their efficient dissociation and recycling. |
| autophagy | The cellular catabolic process in which cells digest parts of their own cytoplasm; allows for both recycling of macromolecular constituents under conditions of cellular stress and remodeling the intracellular structure for cell differentiation. |
| endosome to lysosome transport | The directed movement of substances from endosomes to lysosomes. |
| endosome transport via multivesicular body sorting pathway | The directed movement of substances from endosomes to lysosomes or vacuoles by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the target compartment. |
| late endosome to lysosome transport | The directed movement of substances from late endosome to lysosome. |
| late endosome to vacuole transport | The directed movement of substances from late endosomes to the vacuole. In yeast, after transport to the prevacuolar compartment, endocytic content is delivered to the late endosome and on to the vacuole. This pathway is analogous to endosome to lysosome transport. |
| membrane fission | A process that is carried out at the cellular level which results in the separation of a single continuous membrane into two membranes. |
| midbody abscission | The process by which the midbody, the cytoplasmic bridge that connects the two prospective daughter cells, is severed at the end of mitotic cytokinesis, resulting in two separate daughter cells. |
| mitotic metaphase chromosome alignment | The cell cycle process in which chromosomes are aligned at the metaphase plate, a plane halfway between the poles of the mitotic spindle, during mitosis. |
| multivesicular body assembly | The aggregation, arrangement and bonding together of a set of components to form a multivesicular body, a type of late endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm. |
| multivesicular body sorting pathway | A vesicle-mediated transport process in which transmembrane proteins are ubiquitylated to facilitate their entry into luminal vesicles of multivesicular bodies (MVBs); upon subsequent fusion of MVBs with lysosomes or vacuoles, the cargo proteins are degraded. |
| multivesicular body-lysosome fusion | The organelle membrane fusion process in which the membrane of a multivesicular body fuses with a lysosome to create a hybrid organelle. |
| nuclear membrane reassembly | The reformation of the nuclear membranes following their breakdown in the context of a normal process. |
| nucleus organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the nucleus. |
| plasma membrane repair | The resealing of a cell plasma membrane after cellular wounding due to, for instance, mechanical stress. |
| positive regulation of cytokinesis | Any process that activates or increases the frequency, rate or extent of the division of the cytoplasm of a cell, and its separation into two daughter cells. |
| protein polymerization | The process of creating protein polymers, compounds composed of a large number of component monomers; polymeric proteins may be made up of different or identical monomers. Polymerization occurs by the addition of extra monomers to an existing poly- or oligomeric protein. |
| protein transport | The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. |
| regulation of centrosome duplication | Any process that modulates the frequency, rate or extent of centrosome duplication. Centrosome duplication is the replication of a centrosome, a structure comprised of a pair of centrioles and peri-centriolar material from which a microtubule spindle apparatus is organized. |
| regulation of early endosome to late endosome transport | Any process that modulates the frequency, rate or extent of early endosome to late endosome transport. |
| regulation of endosome size | Any process that modulates the volume of an endosome, a membrane-bounded organelle that carries materials newly ingested by endocytosis. |
| regulation of mitotic spindle assembly | Any process that modulates the frequency, rate or extent of mitotic spindle assembly. |
| suppression of viral release by host | A process in which a host organism stops, prevents or reduces the frequency, rate or extent of the release of a virus with which it is infected, from its cells. |
| ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | The chemical reactions and pathways resulting in the breakdown of a protein or peptide covalently tagged with ubiquitin, via the multivesicular body (MVB) sorting pathway; ubiquitin-tagged proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation. |
| vesicle fusion with vacuole | The joining of the lipid bilayer membrane around a vesicle with the lipid bilayer membrane around the vacuole. |
| viral budding from plasma membrane | A viral budding that starts with formation of a curvature in the host plasma membrane around which the virion particle assembles. |
| viral budding via host ESCRT complex | Viral budding which uses a host ESCRT protein complex, or complexes, to mediate the budding process. |
8 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q58CS7 | CHMP3 | Charged multivesicular body protein 3 | Bos taurus (Bovine) | SS |
| Q9Y3E7 | CHMP3 | Charged multivesicular body protein 3 | Homo sapiens (Human) | EV |
| Q9DB34 | Chmp2a | Charged multivesicular body protein 2a | Mus musculus (Mouse) | SS |
| Q9CQD4 | Chmp1b2 | Charged multivesicular body protein 1b-2 | Mus musculus (Mouse) | PR |
| Q99LU0 | Chmp1b1 | Charged multivesicular body protein 1b-1 | Mus musculus (Mouse) | PR |
| Q8CGS4 | Chmp3 | Charged multivesicular body protein 3 | Rattus norvegicus (Rat) | SS |
| Q5BKM3 | chmp3 | Charged multivesicular body protein 3 | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | SS |
| Q6NY88 | chmp3 | Charged multivesicular body protein 3 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MGLFGKTQEK | PPKELVNEWS | LKIRKEMRVV | DRQIRDIQRE | EEKVKRSVKD | AAKKGQKEVC |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VVLAKEMIRS | RKAVSKLYAS | KAHMNSVLMG | MKNQLAVLRV | AGSLQKSTEV | MKAMQSLVKI |
| 130 | 140 | 150 | 160 | 170 | 180 |
| PEIQATMREL | SKEMMKAGII | EEMLEDTFES | MDDQEEMEEA | AEMEIDRILF | EITAGALGKA |
| 190 | 200 | 210 | 220 | ||
| PSKVTDALPE | PEPAGAMAAS | EEGEEEEDEE | DLEAMQSRLA | TLRS |