Q99JE6
SS
Gene name |
Plcb3 |
Protein name |
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 |
Names |
EC 3.1.4.11 , Phosphoinositide phospholipase C-beta-3 , Phospholipase C-beta-3 , PLC-beta-3 |
Species |
Rattus norvegicus (Rat) |
KEGG Pathway |
|
EC number |
3.1.4.11: Phosphoric diester hydrolases |
Protein Class |
|
Descriptions
PLCB1 catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), and mediates intracellular signaling downstream of G protein-coupled receptors. Autoinhibitory region is a portion of the linker that separates the conserved X and Y boxes comprising the catalytic TIM barrel, at residues 468-539, and occludes the active site of PLCB1. The active site of a PLC isozyme toward phospholipid membranes should force the negatively charged X/Y linker away from the active site, therein relieving autoinhibition of the enzyme.
Autoinhibitory domains (AIDs)
Target domain |
315-705 (TIM barrel) |
Relief mechanism |
Partner binding |
Assay |
|
Target domain |
710-718 (Cleft between the TIM barrel and C2 domains of the catalytic core) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Hicks SN et al. (2008) "General and versatile autoinhibition of PLC isozymes", Molecular cell, 31, 383-94
- Lyon AM et al. (2014) "Molecular mechanisms of phospholipase C β3 autoinhibition", Structure (London, England : 1993), 22, 1844-1854
- Muralidharan K et al. (2021) "Structure and regulation of phospholipase Cβ and ε at the membrane", Chemistry and physics of lipids, 235, 105050
- Charpentier TH et al. (2014) "Membrane-induced allosteric control of phospholipase C-β isozymes", The Journal of biological chemistry, 289, 29545-57
- Lyon AM et al. (2011) "An autoinhibitory helix in the C-terminal region of phospholipase C-β mediates Gαq activation", Nature structural & molecular biology, 18, 999-1005
Autoinhibited structure
Activated structure
1 structures for Q99JE6
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q99JE6-F1 | Predicted | AlphaFoldDB |
No variants for Q99JE6
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q99JE6 | |||||
No associated diseases with Q99JE6
6 regional properties for Q99JE6
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | C2 domain | 706 - 834 | IPR000008 |
| domain | Phosphatidylinositol-specific phospholipase C, X domain | 315 - 467 | IPR000909 |
| domain | Phospholipase C, phosphatidylinositol-specific, Y domain | 589 - 705 | IPR001711 |
| domain | Phospholipase C-beta, C-terminal domain | 943 - 1200 | IPR014815 |
| domain | PLC-beta, PH domain | 18 - 147 | IPR037862 |
| domain | Phosphoinositide phospholipase C beta 1-4-like, EF-hand domain | 152 - 219 | IPR053945 |
Functions
| Description | ||
|---|---|---|
| EC Number | 3.1.4.11 | Phosphoric diester hydrolases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| postsynaptic cytosol | The region of the cytosol consisting of all cytosol that is part of the postsynapse. |
| protein-containing complex | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together. |
| sarcolemma | The outer membrane of a muscle cell, consisting of the plasma membrane, a covering basement membrane (about 100 nm thick and sometimes common to more than one fiber), and the associated loose network of collagen fibers. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| calcium ion binding | Binding to a calcium ion (Ca2+). |
| calmodulin binding | Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states. |
| molecular adaptor activity | The binding activity of a molecule that brings together two or more molecules through a selective, non-covalent, often stoichiometric interaction, permitting those molecules to function in a coordinated way. |
| molecular function activator activity | A molecular function regulator that activates or increases the activity of its target via non-covalent binding that does not result in covalent modification to the target. |
| phosphatidylinositol phospholipase C activity | Catalysis of the reaction |
| phospholipase C activity | Catalysis of the reaction |
9 GO annotations of biological process
| Name | Definition |
|---|---|
| G protein-coupled receptor signaling pathway | The series of molecular signals initiated by a ligand binding to its receptor, in which the activated receptor promotes the exchange of GDP for GTP on the alpha-subunit of an associated heterotrimeric G-protein complex. The GTP-bound activated alpha-G-protein then dissociates from the beta- and gamma-subunits to further transmit the signal within the cell. The pathway begins with receptor-ligand interaction, and ends with regulation of a downstream cellular process. The pathway can start from the plasma membrane, Golgi or nuclear membrane. |
| inositol trisphosphate metabolic process | The chemical reactions and pathways involving myo-inositol phosphate, 1,2,3,4,5,6-cyclohexanehexol, with three phosphate groups attached. |
| phosphatidylinositol catabolic process | The chemical reactions and pathways resulting in the breakdown of phosphatidylinositol, any glycophospholipid with its sn-glycerol 3-phosphate residue is esterified to the 1-hydroxyl group of 1D-myo-inositol. |
| phosphatidylinositol metabolic process | The chemical reactions and pathways involving phosphatidylinositol, any glycophospholipid in which a sn-glycerol 3-phosphate residue is esterified to the 1-hydroxyl group of 1D-myo-inositol. |
| phosphatidylinositol-mediated signaling | The series of molecular signals in which a cell uses a phosphatidylinositol-mediated signaling to convert a signal into a response. Phosphatidylinositols include phosphatidylinositol (PtdIns) and its phosphorylated derivatives. |
| phospholipase C-activating G protein-coupled receptor signaling pathway | A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation of phospholipase C (PLC) and a subsequent increase in the intracellular concentration of inositol trisphosphate (IP3) and diacylglycerol (DAG). |
| post-Golgi vesicle-mediated transport | The directed movement of substances from the Golgi to other parts of the cell, including organelles and the plasma membrane, mediated by small transport vesicles. |
| regulation of systemic arterial blood pressure | The process that modulates the force with which blood travels through the systemic arterial circulatory system. The process is controlled by a balance of processes that increase pressure and decrease pressure. |
| release of sequestered calcium ion into cytosol | The process in which calcium ions sequestered in the endoplasmic reticulum, Golgi apparatus or mitochondria are released into the cytosolic compartment. |
23 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P32383 | PLC1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P10894 | PLCB1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 | Bos taurus (Bovine) | SS |
| Q00722 | PLCB2 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 | Homo sapiens (Human) | EV |
| Q9NQ66 | PLCB1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 | Homo sapiens (Human) | EV |
| Q15147 | PLCB4 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 | Homo sapiens (Human) | PR |
| Q01970 | PLCB3 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 | Homo sapiens (Human) | EV |
| A3KGF7 | Plcb2 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 | Mus musculus (Mouse) | PR |
| Q9Z1B3 | Plcb1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 | Mus musculus (Mouse) | SS |
| P51432 | Plcb3 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 | Mus musculus (Mouse) | SS |
| P10687 | Plcb1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 | Rattus norvegicus (Rat) | SS |
| P24135 | Plcg2 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 | Rattus norvegicus (Rat) | SS |
| P10686 | Plcg1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 | Rattus norvegicus (Rat) | EV |
| P10688 | Plcd1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 | Rattus norvegicus (Rat) | SS |
| Q99P84 | Plce1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 | Rattus norvegicus (Rat) | EV |
| O89040 | Plcb2 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 | Rattus norvegicus (Rat) | PR |
| G5EBH0 | egl-8 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 | Caenorhabditis elegans | SS |
| Q8GV43 | PLC6 | Phosphoinositide phospholipase C 6 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q6NMA7 | PLC9 | Phosphoinositide phospholipase C 9 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9STZ3 | PLC8 | Phosphoinositide phospholipase C 8 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q56W08 | PLC3 | Phosphoinositide phospholipase C 3 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q39032 | PLC1 | Phosphoinositide phospholipase C 1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q944C2 | PLC5 | Phosphoinositide phospholipase C 5 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q944C1 | PLC4 | Phosphoinositide phospholipase C 4 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAGARPGVHA | LQLEPPTVVE | TLRRGSKFIK | WDEEASSRNL | VTLRLDPNGF | FLYWTGPNME |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VDTLDISSIR | DTRTGRYARL | PKDPKIREVL | GFGGPDTRLE | EKLMTVVAGP | DPVNTTFLNF |
| 130 | 140 | 150 | 160 | 170 | 180 |
| MAVQDDTVKV | WSEELFKLAM | NILAQNAPEH | VLRKAYTKLK | LQVNQDGRIP | VKNILKMFSA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| DKKRVETALI | CGLNFNRSES | IRPDEFSLEI | FERFLNKLLL | RPDIDKILLE | IGAKGKPYLT |
| 250 | 260 | 270 | 280 | 290 | 300 |
| LEQLMDFINQ | KQRDPRLNEV | LYPPLRSSQA | RLLIEKYEPN | KQFLERDQMS | MEGFSRYLGG |
| 310 | 320 | 330 | 340 | 350 | 360 |
| EENGILPLEA | LDLSMDMTQP | LSAYFINSSH | NTYLTAGQLA | GTSSVEMYRQ | ALLWGCRCVE |
| 370 | 380 | 390 | 400 | 410 | 420 |
| LDVWKGRPPE | EEPFITHGFT | MTTEVPLRDV | LEAIAETAFK | TSPYPVILSF | ENHVDSAKQQ |
| 430 | 440 | 450 | 460 | 470 | 480 |
| AKMAEYCRSI | FGEALLIDPL | DKYPLSAGTP | LPSPQDLMGR | ILVKNKKRHR | PSTGVPDSSV |
| 490 | 500 | 510 | 520 | 530 | 540 |
| AKRPLEQSNS | ALSESSAATE | PSSPQLGSPS | SDSCPGLSNG | EEVGLEKTSL | EPQKSLGEEG |
| 550 | 560 | 570 | 580 | 590 | 600 |
| LNRGPNVLMP | DRDREDEEED | EEEEETTDPK | KPTTDEGTAS | SEVNATEEMS | TLVNYVEPVK |
| 610 | 620 | 630 | 640 | 650 | 660 |
| FKSFEASRKR | NKCFEMSSFV | ETKAMEQLTK | SPMEFVEYNK | QQLSRIYPKG | TRVDSSNYMP |
| 670 | 680 | 690 | 700 | 710 | 720 |
| QLFWNVGCQL | VALNFQTLDL | PMQLNAGVFE | YNGRSGYLLK | PEFMRRPDKS | FDPFTEVIVD |
| 730 | 740 | 750 | 760 | 770 | 780 |
| GIVANALRVK | VISGQFLSDR | KVGIYVEVDM | FGLPVDTRRK | YRTRTSQGNS | FNPVWDEEPF |
| 790 | 800 | 810 | 820 | 830 | 840 |
| DFPKVVLPTL | ASLRIAAFEE | GGRFVGHRIL | PVSAIRSGYH | YVCLRNEANQ | PLCLPALLIY |
| 850 | 860 | 870 | 880 | 890 | 900 |
| TEASDYIPDD | HQDYAEALIN | PIKHVSLMDQ | RAKQLAALIG | ESEAQASTEM | CQETPSQQQG |
| 910 | 920 | 930 | 940 | 950 | 960 |
| SQLSSNPVPN | PLDDSPRWPP | GPTTSPTSTS | LSSPGQRDDL | IASILSEVTP | TPLEELRSHK |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| AMVKLRSRQD | RDLRELHKKH | QRKAVALTRR | LLDGLAQARA | EGKCRPSSSA | LSRATNVEDV |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| KEEEKEAARQ | YREFQNRQVQ | SLLELREAQA | DAETERRLEH | LKQAQQRLRE | VVLDAHTTQF |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| KRLKELNERE | KKELQKILDR | KRNNSISEAK | TREKHKKEVE | LTEINRRHIT | ESVNSIRRLE |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| EAQKQRHERL | LAGQQQVLQQ | LVEEEPKLVA | QLTQECQEQR | ERLPQEIRRC | LLGETSEGLG |
| 1210 | 1220 | 1230 | |||
| DGPLVACASN | GHAAGSGGHQ | SGADSESQEE | NTQL |