Q99JD4

SS
Gene name
Clasp2
Protein name
CLIP-associating protein 2
Names
Cytoplasmic linker-associated protein 2
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:114514
EC number
Protein Class

Descriptions

CLASPs, major microtubule-stabilizing factors in interphase and mitosis, prevent microtubule from switching form growth to shortening by stabilizing growing microtubule ends, and thus suppress microtubule catastrophes. CLASP2a consists of three TOG-like domains (termed TOG1, 2, and 3) and a C-terminal domain, CLIP-interacting domain (CLIP-ID) responsible for interaction with CLIP-170 and other partners. CLASP2a TOG2 is necessary and sufficient for catastrophe inhibition. The inhibition function is suppressed by the C-terminal domain CLIP-ID while the TOG1 domain or the CLIP-ID partner binding to CLIP-ID can release the autoinhibition.

Autoinhibitory domains (AIDs)

1169-1278 (C-terminal domain CLIP-ID) SS

Target domain

640-877 (TOG2 domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q99JD4

Entry ID Method Resolution Chain Position Source
AF-Q99JD4-F1 Predicted AlphaFoldDB

No variants for Q99JD4

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q99JD4

No associated diseases with Q99JD4

4 regional properties for Q99JD4

Type Name Position InterPro Accession
domain CLASP N-terminal domain 94 - 311 IPR024395
domain TOG domain 90 - 323 IPR034085-1
domain TOG domain 640 - 877 IPR034085-2
domain TOG domain 1043 - 1274 IPR034085-3

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Chromosome, centromere, kinetochore
  • Cytoplasm, cytoskeleton, spindle
  • Golgi apparatus
  • Golgi apparatus, trans-Golgi network
  • Cell membrane
  • Cell projection, ruffle membrane
  • Localizes to microtubule plus ends
  • Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase
  • Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase
  • In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella
  • Localizes to the cell cortex and this requires ERC1 and PHLDB2
  • The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

15 GO annotations of cellular component

Name Definition
basal cortex The region that lies just beneath the plasma membrane on the basal edge of a cell.
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cell leading edge The area of a motile cell closest to the direction of movement.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasmic microtubule Any microtubule in the cytoplasm of a cell.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.
microtubule cytoskeleton The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
mitotic spindle A spindle that forms as part of mitosis. Mitotic and meiotic spindles contain distinctive complements of proteins associated with microtubules.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
ruffle membrane The portion of the plasma membrane surrounding a ruffle.
spindle microtubule Any microtubule that is part of a mitotic or meiotic spindle; anchored at one spindle pole.
trans-Golgi network The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side distal to the endoplasmic reticulum, from which secretory vesicles emerge. The trans-Golgi network is important in the later stages of protein secretion where it is thought to play a key role in the sorting and targeting of secreted proteins to the correct destination.

4 GO annotations of molecular function

Name Definition
dystroglycan binding Binding to dystroglycan, a glycoprotein found in non-muscle tissues as well as in muscle tissues, often in association with dystrophin. The native dystroglycan cleaved into two non-covalently associated subunits, alpha (N-terminal) and beta (C-terminal).
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule plus-end binding Binding to the plus end of a microtubule.
protein tyrosine kinase binding Binding to protein tyrosine kinase.

31 GO annotations of biological process

Name Definition
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues.
cellular response to insulin stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms.
establishment of cell polarity The specification and formation of anisotropic intracellular organization or cell growth patterns.
establishment of mitotic spindle localization The cell cycle process in which the directed movement of the mitotic spindle to a specific location in the cell occurs.
establishment or maintenance of cell polarity Any cellular process that results in the specification, formation or maintenance of anisotropic intracellular organization or cell growth patterns.
exit from mitosis The cell cycle transition where a cell leaves M phase and enters a new G1 phase. M phase is the part of the mitotic cell cycle during which mitosis and cytokinesis take place.
fibroblast migration Cell migration that is accomplished by extension and retraction of a fibroblast pseudopodium. A fibroblast is a connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules.
Golgi organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the Golgi apparatus.
microtubule anchoring Any process in which a microtubule is maintained in a specific location in a cell.
microtubule cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins.
microtubule depolymerization The removal of tubulin heterodimers from one or both ends of a microtubule.
microtubule nucleation The process in which tubulin alpha-beta heterodimers begin aggregation to form an oligomeric tubulin structure (a microtubule seed). Microtubule nucleation is the initiating step in the formation of a microtubule in the absence of any existing microtubules ('de novo' microtubule formation).
microtubule organizing center organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a microtubule organizing center, a structure from which microtubules grow.
mitotic spindle assembly Mitotic bipolar spindle assembly begins with spindle microtubule nucleation from the separated spindle pole body, includes spindle elongation during prometaphase, and is complete when all kinetochores are stably attached the spindle, and the spindle assembly checkpoint is satisfied.
mitotic spindle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
negative regulation of focal adhesion assembly Any process that stops, prevents, or reduces the frequency, rate or extent of focal adhesion assembly, the establishment and maturation of focal adhesions.
negative regulation of microtubule depolymerization Any process that stops, prevents, or reduces the frequency, rate or extent of microtubule depolymerization; prevention of depolymerization of a microtubule can result from binding by 'capping' at the plus end (e.g. by interaction with another cellular protein of structure) or by exposing microtubules to a stabilizing drug such as taxol.
negative regulation of stress fiber assembly Any process that stops, prevents, or reduces the frequency, rate or extent of the assembly a stress fiber, a bundle of microfilaments and other proteins found in fibroblasts.
negative regulation of wound healing, spreading of epidermal cells Any process that stops, prevents or reduces the frequency, rate or extent of wound healing, spreading of epidermal cells.
positive regulation of basement membrane assembly involved in embryonic body morphogenesis Any process that activates or increases the frequency, rate or extent of basement membrane assembly involved in embryonic body morphogenesis.
positive regulation of epithelial cell migration Any process that activates or increases the frequency, rate or extent of epithelial cell migration.
positive regulation of exocytosis Any process that activates or increases the frequency, rate or extent of exocytosis.
positive regulation of extracellular matrix disassembly Any process that increases the rate, frequency or extent of extracellular matrix disassembly. Extracellular matrix disassembly is a process that results in the breakdown of the extracellular matrix.
positive regulation of protein localization to membrane Any process that activates or increases the frequency, rate or extent of protein localization to membrane.
protein localization to plasma membrane A process in which a protein is transported to, or maintained in, a specific location in the plasma membrane.
regulation of epithelial to mesenchymal transition Any process that modulates the rate, frequency, or extent of epithelial to mesenchymal transition. Epithelial to mesenchymal transition where an epithelial cell loses apical/basolateral polarity, severs intercellular adhesive junctions, degrades basement membrane components and becomes a migratory mesenchymal cell.
regulation of gastrulation Any process that modulates the rate or extent of gastrulation. Gastrulation is the complex and coordinated series of cellular movements that occurs at the end of cleavage during embryonic development of most animals.
regulation of microtubule polymerization or depolymerization Any process that modulates the frequency, rate or extent of microtubule polymerization or depolymerization by the addition or removal of tubulin heterodimers from a microtubule.
regulation of microtubule-based process Any process that modulates the frequency, rate or extent of any cellular process that depends upon or alters the microtubule cytoskeleton.
vesicle targeting The process in which vesicles are directed to specific destination membranes. Targeting involves coordinated interactions among cytoskeletal elements (microtubules or actin filaments), motor proteins, molecules at the vesicle membrane and target membrane surfaces, and vesicle cargo.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P38198 STU1 Protein STU1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q9NBD7 chb CLIP-associating protein Drosophila melanogaster (Fruit fly) SS
Q7Z460 CLASP1 CLIP-associating protein 1 Homo sapiens (Human) SS
O75122 CLASP2 CLIP-associating protein 2 Homo sapiens (Human) EV
Q80TV8 Clasp1 CLIP-associating protein 1 Mus musculus (Mouse) SS
Q8BRT1 Clasp2 CLIP-associating protein 2 Mus musculus (Mouse) SS
Q6NYW6 clasp2 CLIP-associating protein 2 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MRRLICKRIC DYKSFDDEES VDGNRPSSAA SAFKVPAPKT PGNPVNSARK PGSAGGPKAG
70 80 90 100 110 120
GTSKEGGAGA VDEDDFIKAF TDVPSIQIYS SRELEETLNK IREILSDDKH DWDQRANALK
130 140 150 160 170 180
KIRSLLVAGA AQYDCFFQHL RLLDGALKLS AKDLRSQVVR EACITVAHLS TVLGNKFDHG
190 200 210 220 230 240
AEAIVPTLFN LVPNSAKVMA TSGCAAIRFI IRHTHVPRLI PLITSNCTSK SVPVRRRSFE
250 260 270 280 290 300
FLDLLLQEWQ THSLERHAAV LVETIKKGIH DADAEARVEA RKTYMGLRNH FPGEAETLYN
310 320 330 340 350 360
SLEPSYQKSL QTYLKSSGSV ASLPQSDRSS SSSQESLNRP FSSKWSTANP SAVAGRVSVG
370 380 390 400 410 420
GSKASPLPGS LQRSRSDIDV NAAAGAKAHH AAGQAVRSGR LGAGALNPGS YASLEDTSDK
430 440 450 460 470 480
MDGTASEDGR VRAKLSTPLV AVGNAKTDSR GRSRTKMVSQ SQPGSRSGSP GRVLTTTALS
490 500 510 520 530 540
TVSSGAQRIL VNSASAQKRS KIPRSQGCSR EASPSRLSVA RSSRIPRPSV SQGCSREASR
550 560 570 580 590 600
ESSRDTSPVR SFQPLGPGYG MSQSSRLSSS VSAMRVLNTG SDVEEAVADA LLLGDIRTKK
610 620 630 640 650 660
KPARRRYESY GMHSDDDANS DASSACSERS YSSRNGSIPT YMRQTEDVAE VLNRCASSNW
670 680 690 700 710 720
SERKEGLLGL QNLLKNQRTL SRVELKRLCE IFTRMFADPH GKVFSMFLET LVDFIQVHKD
730 740 750 760 770 780
DLQDWLFVLL TQLLKKMGAD LLGSVQAKVQ KALDVTRESF PNDLQFNILM RFTVDQTQTP
790 800 810 820 830 840
SLKVKVAILK YIETLAKQMD PGDFINSSET RLAVSRVITW TTEPKSSDVR KAAQSVLISL
850 860 870 880 890 900
FELNTPEFTM LLGALPKTFQ DGATKLLHNH LRNTGNGTQS SMGSPLTRPT PRSPANWSSP
910 920 930 940 950 960
LTSPTNTSQN TLSPSAFDYD TENMNSEDIY SSLRGVTEAI QNFSFRSQED MSEPLKRDPK
970 980 990 1000 1010 1020
KEDGDTVCSG PGMSDPRAGG DAPDSSQPAL DNKASLLHSV PLHSSPRSRD YNPYNYSDSI
1030 1040 1050 1060 1070 1080
SPFNKSALKE AMFDDDADQF PDDLSLDHSD LVAELLKELS NHNERIEERK IALYELMKLT
1090 1100 1110 1120 1130 1140
QEESFSVWDE HFKTILLLLL ETLGDKEPTI RALALKVLKE ILRHQPARFK NYAELTVMKT
1150 1160 1170 1180 1190 1200
LEAHKDPHKE VVRSAEEAAS VLATSISPEQ CIKVLCPIIQ TADYPINLAA IKMQTKVIER
1210 1220 1230 1240 1250 1260
VSKETLNLLL PEIMPGLIQG YDNSESSVRK ACVFCLVAVH AVIGDELKPH LSQLTGSKMK
1270 1280
LLNLYIKRAQ TGSAGADPTT DVSGQS