AiPD: Autoinhibited Protein Database

Q96RU8

Gene name	TRIB1
Protein name	Tribbles homolog 1
Names	TRB-1, G-protein-coupled receptor-induced gene 2 protein, GIG-2, SKIP1
Species	Homo sapiens (Human)
KEGG Pathway	hsa:10221
EC number
Protein Class	SER/THR PROTEIN KINASE-TRB (PTHR22961)

Descriptions

TRIB1 controls the levels of C/EBPs involved in the differentiation of myeloid cells and adipocytes by recruiting the proteins to COP1 ubiquitin ligase. TRIB1 recognizes a conserved stretch within a transactivation domain of C/EBP proteins, and suggests a model by which TRIB1 acts as a dynamic adapter for recruiting C/EBPs to COP1. TRIB1 contains a pseudokinase domain and this pseudokinase interacts with the COP1-binding motif and prevents the recruitment of COP1 ubiquitin ligase.

Autoinhibitory domains (AIDs)

Target domain	355-360 (COP1-binding motif)
Relief mechanism	PTM
Assay	Deletion assay, Mutagenesis experiment, Structural analysis

AID

1-338 (Pseudokinase domain)

Target domain

355-360 (COP1-binding motif)

Relief mechanism

PTM (RIPK3-mediated phosphorylation of pseudokinase domain)

Assay

Deletion assay, Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Murphy JM et al. (2015) "Molecular Mechanism of CCAAT-Enhancer Binding Protein Recruitment by the TRIB1 Pseudokinase", Structure (London, England : 1993), 23, 2111-21

Jamieson SA et al. (2018) "Substrate binding allosterically relieves autoinhibition of the pseudokinase TRIB1", Science signaling, 11,

Autoinhibited structure

Activated structure

6 structures for Q96RU8

Entry ID	Method	Resolution	Chain	Position	Source
5CEK	X-ray	280 A	A	83-343	PDB
5CEM	X-ray	210 A	A	83-371	PDB
5IGO	X-ray	160 A	U/V/W/X	354-361	PDB
5IGQ	X-ray	390 A			PDB
6DC0	X-ray	280 A	A/B	90-343	PDB
AF-Q96RU8-F1	Predicted				AlphaFoldDB

271 variants for Q96RU8

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA372271167 rs988507358	2	R>L		No	ClinGen TOPMed gnomAD
CA372271166 rs988507358	2	R>P		No	ClinGen TOPMed gnomAD
CA185505449 rs988507358	2	R>Q		No	ClinGen TOPMed gnomAD
CA372271165 rs1320383987	2	R>W		No	ClinGen gnomAD
CA372271186 rs1214319185	6	V>M		No	ClinGen TOPMed
CA372271195 rs1266069049	7	R>H		No	ClinGen TOPMed gnomAD
CA4874503 rs778095820	8	S>A		No	ClinGen ExAC gnomAD
rs1300381744 CA372271202	8	S>F		No	ClinGen TOPMed
rs1176986805 CA372271208	9	A>V		No	ClinGen TOPMed gnomAD
CA372271209 rs914165832	10	M>L		No	ClinGen TOPMed
rs914165832 CA185505450	10	M>V		No	ClinGen TOPMed
rs1440081687 CA372271225	12	G>S		No	ClinGen gnomAD
rs1392498906 CA372271239	14	S>P		No	ClinGen TOPMed gnomAD
rs1180210350 CA372271255	16	P>R		No	ClinGen TOPMed gnomAD
CA185505451 rs968674221	18	G>S		No	ClinGen TOPMed
rs747946279 CA4874504	18	G>V		No	ClinGen ExAC TOPMed gnomAD
CA372271272 rs1318985802	19	P>L		No	ClinGen TOPMed gnomAD
rs1390586417 CA372271269	19	P>S		No	ClinGen TOPMed
rs771096580 CA4874505	20	A>S		No	ClinGen ExAC TOPMed gnomAD
CA372271281 rs1351095091	21	L>P		No	ClinGen gnomAD
CA372271285 rs1181912608	22	L>F		No	ClinGen TOPMed
rs1483969996 CA372271287	22	L>P		No	ClinGen TOPMed
CA372271299 rs1448000354	24	P>S		No	ClinGen TOPMed gnomAD
rs1277221139 CA372271316	27	R>*		No	ClinGen TOPMed
CA372271318 rs1039513360	27	R>L		No	ClinGen TOPMed
CA185505452 rs1039513360	27	R>P		No	ClinGen TOPMed
CA372271317 rs1039513360	27	R>Q		No	ClinGen TOPMed
rs1314130016 CA372271327	29	V>F		No	ClinGen TOPMed
rs1297777136 CA372271339	31	A>S		No	ClinGen TOPMed
rs1014314547 CA185505455	33	R>S		No	ClinGen TOPMed
CA185505457 rs1047143776	37	A>D		No	ClinGen gnomAD
rs1023068171 CA185505458	38	D>H		No	ClinGen TOPMed
rs1294779641 CA372271405	41	A>V		No	ClinGen TOPMed gnomAD
rs770314650 CA4874508	42	A>S		No	ClinGen ExAC TOPMed gnomAD
rs770314650 CA185505459	42	A>T		No	ClinGen ExAC TOPMed gnomAD
rs775786371 CA4874509	42	A>V		No	ClinGen ExAC gnomAD
CA372271418 rs1235489441	44	A>E		No	ClinGen gnomAD
rs1208735184 CA372271428	46	K>E		No	ClinGen TOPMed gnomAD
CA372271427 rs1208735184	46	K>Q		No	ClinGen TOPMed gnomAD
rs1182365768 CA372271438	47	C>Y		No	ClinGen gnomAD
rs1563823407 CA372271445	48	P>S		No	ClinGen Ensembl
rs1415420971 CA372271449	49	R>C		No	ClinGen TOPMed gnomAD
rs1169272251 CA372271459	50	L>P		No	ClinGen gnomAD
rs1214324206 CA372271465	51	S>C		No	ClinGen TOPMed gnomAD
rs763427037 CA4874510	56	P>S		No	ClinGen ExAC gnomAD
rs1220690805 CA372271526	60	L>F		No	ClinGen TOPMed
rs767050256 CA4874511	62	P>A		No	ClinGen ExAC TOPMed gnomAD
CA185505466 rs965024488	62	P>L		No	ClinGen TOPMed
rs767050256 CA185505465	62	P>S		No	ClinGen ExAC TOPMed gnomAD
rs767050256 CA185505464	62	P>T		No	ClinGen ExAC TOPMed gnomAD
CA372271545 rs1448026346	63	P>H		No	ClinGen TOPMed gnomAD
CA372271544 rs1448026346	63	P>L		No	ClinGen TOPMed gnomAD
CA372271549 rs1381901155	64	G>D		No	ClinGen gnomAD
rs1402829800 CA372271547	64	G>R		No	ClinGen TOPMed
CA372271546 rs1402829800	64	G>S		No	ClinGen TOPMed
CA372271557 rs1216895020	65	S>L		No	ClinGen TOPMed gnomAD
CA372271565 rs1318918952	67	C>R		No	ClinGen gnomAD
CA185505469 rs976444542	67	C>Y		No	ClinGen TOPMed gnomAD
CA372271578 rs2385113	68	S>R		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1001989436 CA185505470	69	P>L		No	ClinGen TOPMed gnomAD
rs1472661390 CA372271594	71	P>S		No	ClinGen TOPMed
rs1586846836 CA372271603	72	P>Q		No	ClinGen Ensembl
CA372271623 rs1413675271	76	P>A		No	ClinGen TOPMed
rs746665161 CA185505472	76	P>L		No	ClinGen TOPMed
rs746665161 CA185505471	76	P>Q		No	ClinGen TOPMed
rs1268515750 CA372271629	77	G>E		No	ClinGen TOPMed
CA185505473 rs1035112785	77	G>R		No	ClinGen TOPMed
CA372271634 rs1423948480	78	A>D		No	ClinGen gnomAD
CA372271633 rs1170439720	78	A>S		No	ClinGen gnomAD
CA372271645 rs1159281309	80	G>R		No	ClinGen gnomAD
rs1410248021 CA372271652	81	G>D		No	ClinGen gnomAD
rs934968372 CA185505475	82	S>F		No	ClinGen TOPMed
CA372271662 rs1399514169	83	G>R		No	ClinGen TOPMed gnomAD
CA4874514 rs765931219 COSM4162667 COSM4162666	84	S>I	thyroid [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs765931219 CA372271669	84	S>N		No	ClinGen ExAC TOPMed gnomAD
rs1281558024 CA372271676	85	A>G		No	ClinGen TOPMed
CA185505477 rs968020124	85	A>T		No	ClinGen Ensembl
CA185505479 rs895790586	87	G>E		No	ClinGen TOPMed
rs1319856589 CA372271704	90	R>C		No	ClinGen TOPMed
rs1297709886 CA372271706	90	R>H		No	ClinGen gnomAD
CA185505481 rs766476837	92	A>S		No	ClinGen Ensembl
CA372271724 rs1586846890	93	D>G		No	ClinGen Ensembl
CA185505482 rs868370890	96	L>M		No	ClinGen Ensembl
CA372271795 rs1402157810	100	A>T		No	ClinGen gnomAD
CA185505483 rs751760974	100	A>V		No	ClinGen gnomAD
rs976624217 CA372271845	103	E>D		No	ClinGen TOPMed gnomAD
CA372271832 COSM1187610 rs1586846901 COSM1187611	103	E>K	lung [Cosmic]	No	ClinGen cosmic curated Ensembl
CA4874515 rs373667881	107	R>L		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA372271890 rs373667881	107	R>Q		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA372271899 rs1427147515	108	A>S		No	ClinGen TOPMed
rs905975357 CA185505487	110	C>G		No	ClinGen TOPMed
CA185505488 rs200584467	112	H>D		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA4874516 rs200584467	112	H>Y		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1466264526 CA372272121	113	T>P		No	ClinGen TOPMed
rs1200012221 CA372272130	114	G>V		No	ClinGen TOPMed
CA185505489 rs770510208	117	L>P		No	ClinGen Ensembl
CA372272154 rs1276883708	118	R>P		No	ClinGen TOPMed
CA185505490 rs1026856398	118	R>S		No	ClinGen TOPMed
CA372272171 rs1219608959	120	K>N		No	ClinGen gnomAD
rs1406233688 CA372272197	122	F>L		No	ClinGen gnomAD
CA372272194 rs1236402495	122	F>S		No	ClinGen TOPMed gnomAD
rs1182459126 CA372272203	123	P>L		No	ClinGen TOPMed
rs1398303413 CA372272210	124	I>S		No	ClinGen gnomAD
rs1445798491 CA372272227	126	H>Q		No	ClinGen gnomAD
CA4874545 rs201548983	127	Y>H		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA4874546 rs750474120	134	Y>C		No	ClinGen ExAC TOPMed gnomAD
rs1242488491 CA372272281	134	Y>H		No	ClinGen gnomAD
CA4874547 rs756257872	136	Q>R		No	ClinGen ExAC gnomAD
CA372272301 rs1211040690	137	L>V		No	ClinGen gnomAD
rs1457004284 CA372272316	139	S>L		No	ClinGen gnomAD
CA4874549 rs749750278	142	N>K		No	ClinGen ExAC gnomAD
CA372272343 rs1440108532	143	I>N		No	ClinGen TOPMed gnomAD
CA372272345 rs1440108532	143	I>T		No	ClinGen TOPMed gnomAD
CA372272342 rs1239143332	143	I>V		No	ClinGen gnomAD
CA185505741 rs1053517987	144	T>I		No	ClinGen Ensembl
CA4874550 rs200241998	149	V>E		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA372272388 rs1280588367	150	I>S		No	ClinGen TOPMed
CA372272415 rs1473173183	154	T>S		No	ClinGen gnomAD
CA4874551 rs779298762	157	Y>C		No	ClinGen ExAC gnomAD
CA4874555 rs369764683	161	E>K		No	ClinGen ESP ExAC TOPMed gnomAD
rs769371321 CA4874556	162	K>N		No	ClinGen ExAC TOPMed gnomAD
rs774867059 CA4874557	164	F>S		No	ClinGen ExAC gnomAD
rs1441792790 CA372272492	165	G>E		No	ClinGen TOPMed
rs1398682969 CA372272506	167	M>I		No	ClinGen TOPMed
rs751349356 CA372272504	167	M>K		No	ClinGen ExAC TOPMed gnomAD
CA4874559 rs762906722	167	M>L		No	ClinGen ExAC TOPMed gnomAD
CA4874560 rs751349356	167	M>T		No	ClinGen ExAC TOPMed gnomAD
rs762906722 CA4874558	167	M>V		No	ClinGen ExAC TOPMed gnomAD
CA372272511 rs1406966110	168	H>N		No	ClinGen TOPMed
rs761638692 CA185505743	168	H>P		No	ClinGen ExAC gnomAD
rs761638692 CA4874561	168	H>R		No	ClinGen ExAC gnomAD
rs750702470 CA4874563	170	Y>H		No	ClinGen ExAC gnomAD
rs1586848261 CA372272531	171	V>E		No	ClinGen Ensembl
rs1199031481 CA372272538	172	R>Q		No	ClinGen gnomAD
rs56285697 CA4874565 VAR_042364	173	S>R		No	ClinGen UniProt ExAC dbSNP gnomAD
rs755466534 CA4874567	174	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA4874566 rs758099027	174	R>W		No	ClinGen ExAC TOPMed gnomAD
CA372272559 rs1468563216	176	R>K		No	ClinGen TOPMed
rs1477651120 CA372272567	177	L>P		No	ClinGen gnomAD
CA4874569 rs748516687	178	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA4874568 rs779326105 COSM1095978	178	R>W	ovary endometrium bone [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA372272600 rs1285389554	182	A>G		No	ClinGen TOPMed
COSM1454767 CA372272601 rs1285389554	182	A>V	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed
CA372272603 rs778292179	183	A>S		No	ClinGen ExAC TOPMed gnomAD
rs778292179 CA4874571	183	A>T		No	ClinGen ExAC TOPMed gnomAD
CA185505745 rs150043688	184	R>Q		No	ClinGen ESP TOPMed gnomAD
CA4874572 rs371769788	184	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
rs267601768 CA185505746	186	F>L		No	ClinGen Ensembl
rs754492794 CA185505747	187	K>E		No	ClinGen Ensembl
rs1295867266 CA372272646	190	V>I		No	ClinGen gnomAD
rs149162682 CA4874574	192	A>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs140863521 CA185505748	193	V>I		No	ClinGen ESP gnomAD
rs796974086 CA185505749	194	A>S		No	ClinGen TOPMed gnomAD
CA372272668 COSM1095980 rs796974086	194	A>T	endometrium [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
rs761583643 CA4874578	195	H>R		No	ClinGen ExAC TOPMed gnomAD
CA372272712 rs1184326541	200	A>S		No	ClinGen TOPMed
CA4874580 rs544694807	201	I>M		No	ClinGen ExAC TOPMed gnomAD
rs767242895 CA4874579	201	I>S		No	ClinGen ExAC TOPMed gnomAD
CA4874582 rs199695896	202	V>M		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA372272742 rs1377970517	205	D>G		No	ClinGen gnomAD
CA372272737 COSM1095981 rs1197954090	205	D>N	endometrium [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
CA372272760 rs1252836124	208	L>F		No	ClinGen gnomAD
CA185505750 rs144721529	209	R>S		No	ClinGen ESP gnomAD
rs1273942367 CA372272786 COSM1095983	212	V>I	endometrium [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
rs1273942367 CA372272787	212	V>L		No	ClinGen TOPMed gnomAD
rs34349706 CA4874588 VAR_042365	215	T>M		No	ClinGen UniProt ESP ExAC TOPMed dbSNP gnomAD
rs958687549 CA185505752	216	E>K		No	ClinGen TOPMed
CA4874592 rs748828188	217	E>A		No	ClinGen ExAC gnomAD
rs531999672 CA4874591	217	E>K		No	ClinGen 1000Genomes ExAC gnomAD
CA372272857 rs1237313025	220	Q>H		No	ClinGen gnomAD
rs1586849487 CA372272867	222	R>K		No	ClinGen Ensembl
CA4874613 rs747683346	224	E>G		No	ClinGen ExAC gnomAD
rs771978463 CA4874614	225	S>G		No	ClinGen ExAC gnomAD
rs746804477 CA4874616	227	E>D		No	ClinGen ExAC TOPMed gnomAD
rs1199942652 CA372272914	229	T>I		No	ClinGen TOPMed
rs1382703413 CA372272939	232	M>I		No	ClinGen TOPMed gnomAD
rs1443651516 CA372272934	232	M>L		No	ClinGen gnomAD
CA372272937 rs1260980798	232	M>R		No	ClinGen TOPMed
CA4874621 rs147892039	234	G>A		No	ClinGen ESP ExAC TOPMed gnomAD
rs147892039 CA4874620	234	G>E		No	ClinGen ESP ExAC TOPMed gnomAD
rs1563825149 CA372272955	235	E>Q		No	ClinGen Ensembl
rs1293948324 CA372273017	243	H>R		No	ClinGen gnomAD
rs1257256547 CA372273054	249	V>M		No	ClinGen gnomAD
rs1230576914 CA372273063	250	S>N		No	ClinGen TOPMed
CA372273081 rs1563825172	252	E>D		No	ClinGen Ensembl
CA372273100 rs1284269862	255	N>K		No	ClinGen TOPMed
CA4874626 rs370525824	255	N>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA4874627 rs767708343	258	G>R		No	ClinGen ExAC gnomAD
rs1294173353 CA372273139	262	G>R		No	ClinGen TOPMed gnomAD
rs1258979472 CA372273164	265	A>V		No	ClinGen gnomAD
VAR_042366 CA4874632 rs56056430	267	V>I		No	ClinGen UniProt 1000Genomes ExAC TOPMed dbSNP gnomAD
rs373747069 CA185506008	273	M>V		No	ClinGen ESP TOPMed
CA4874634 rs746850790	274	L>F		No	ClinGen ExAC TOPMed gnomAD
CA372273237 rs1586849594	276	T>S		No	ClinGen Ensembl
rs555283941 CA4874636	277	L>F		No	ClinGen 1000Genomes ExAC gnomAD
rs770107901 CA4874638	279	V>A		No	ClinGen ExAC gnomAD
CA4874637 rs745723498	279	V>I		No	ClinGen ExAC gnomAD
rs775918973 COSM1095987 CA4874639 COSM1095986	281	R>*	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC
rs200818822 CA185506009	286	D>G		No	ClinGen 1000Genomes
rs1216140089 CA372273333	291	A>V		No	ClinGen TOPMed gnomAD
CA4874643 COSM216807 rs200217434 COSM216806	297	R>W	pancreas endometrium [Cosmic]	No	ClinGen cosmic curated 1000Genomes ExAC TOPMed gnomAD
VAR_042367 rs55953723 CA4874645	298	R>C		No	ClinGen UniProt ExAC TOPMed dbSNP gnomAD
rs55953723 CA4874644	298	R>G		No	ClinGen ExAC TOPMed gnomAD
CA4874646 COSM1095989 COSM1095988 rs151229265	298	R>H	endometrium [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
rs55953723 CA372273374	298	R>S		No	ClinGen ExAC TOPMed gnomAD
rs752285927 CA372273384	300	Q>*		No	ClinGen ExAC TOPMed gnomAD
CA4874648 rs752285927	300	Q>E		No	ClinGen ExAC TOPMed gnomAD
CA372273397 rs151032622	301	F>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs751098238 CA4874651 COSM3382111 COSM3382112	303	I>V	pancreas [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA372273415 rs1423484794	304	P>L		No	ClinGen gnomAD
rs757119456 CA4874652	306	H>Q		No	ClinGen ExAC TOPMed gnomAD
rs756032753 COSM270388 COSM270389 CA4874655	308	S>F	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA372273450 rs1162161939	309	P>L		No	ClinGen gnomAD
rs1346056913 CA372273464	311	A>V		No	ClinGen gnomAD
CA4874659 rs774250787	312	R>G		No	ClinGen ExAC TOPMed gnomAD
CA4874660 rs748313132	313	C>G		No	ClinGen ExAC gnomAD
rs1234672719 CA372273477	313	C>W		No	ClinGen gnomAD
rs772430089 CA4874661	316	R>C		No	ClinGen ExAC TOPMed gnomAD
COSM3432105 CA4874662 rs138022510 COSM3432104	316	R>H	large_intestine [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA4874663 COSM236892 COSM236891 rs761181000	317	S>N	prostate [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA4874666 rs367629272 COSM247971 COSM247972	321	R>Q	prostate [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
COSM1674172 rs777006839 COSM1674171 CA4874665	321	R>W	haematopoietic_and_lymphoid_tissue [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA4874667 rs763855292	323	P>H		No	ClinGen ExAC gnomAD
CA4874668 rs763855292	323	P>L		No	ClinGen ExAC gnomAD
CA185506010 rs865953650	323	P>S		No	ClinGen Ensembl
CA185506011 rs756273869	325	E>*		No	ClinGen ExAC gnomAD
CA4874671 rs756273869	325	E>K		No	ClinGen ExAC gnomAD
rs1018546661 CA185506012	326	R>S		No	ClinGen Ensembl
CA4874672 rs756127642	327	L>P		No	ClinGen ExAC gnomAD
rs1407023166 CA372273565	328	T>I		No	ClinGen TOPMed
CA372273571 rs1586849740	329	A>V		No	ClinGen Ensembl
rs371719876 CA372273573	330	P>A		No	ClinGen ESP ExAC TOPMed gnomAD
rs371719876 CA4874673	330	P>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA372273579 rs1184690907	331	E>A		No	ClinGen TOPMed
CA372273582 rs1422745008	331	E>D		No	ClinGen TOPMed
rs755232835 CA4874675 COSM1733067 COSM1733068	331	E>K	pancreas [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs372024937 CA372273610	336	P>A		No	ClinGen ESP ExAC TOPMed gnomAD
rs1030948982 CA185506014	336	P>R		No	ClinGen TOPMed gnomAD
rs372024937 CA4874677	336	P>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA372273623 rs1399173270	338	F>L		No	ClinGen TOPMed gnomAD
rs142512119 CA4874679	341	V>I		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs908077460 CA185506015	344	P>T		No	ClinGen Ensembl
rs1025680568 CA185506016	345	G>R		No	ClinGen Ensembl
CA4874682 rs776955393	347	I>F		No	ClinGen ExAC gnomAD
COSM1454772 rs776955393 CA4874683 COSM1454773	347	I>V	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs1255258708 CA372273687	348	D>N		No	ClinGen TOPMed gnomAD
rs376927073 CA4874686	349	S>L		No	ClinGen ESP ExAC TOPMed gnomAD
CA185506017 rs770958333	349	S>T		No	ClinGen Ensembl
rs12547645 CA185506018	350	E>K		No	ClinGen Ensembl
CA372273716 rs1410324601	352	G>E		No	ClinGen TOPMed
CA4874687 rs761386608	353	T>I		No	ClinGen ExAC TOPMed gnomAD
rs1161382409 CA372273729	354	S>*		No	ClinGen TOPMed
COSM174413 CA4874688 rs766988397 COSM174412	356	Q>H	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA185506019 rs1017204805	356	Q>L		No	ClinGen Ensembl
VAR_042368 rs35454769 CA4874689	360	E>A		No	ClinGen UniProt ExAC TOPMed dbSNP gnomAD
RCV000962340 CA4874690 VAR_042369 rs16900603	360	E>D		No	ClinGen ClinVar UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA372273764 rs1563825399	360	E>Q		No	ClinGen Ensembl
rs1437386589 CA372273776	361	Y>*		No	ClinGen TOPMed
CA372273778 rs766477822	362	Q>*		No	ClinGen ExAC TOPMed gnomAD
rs766477822 CA4874691	362	Q>E		No	ClinGen ExAC TOPMed gnomAD
rs764074112 CA185506022	362	Q>H		No	ClinGen gnomAD
CA185506021 rs138424670	362	Q>R		No	ClinGen ESP
rs754870885 CA4874693	364	D>E		No	ClinGen ExAC TOPMed gnomAD
CA372273801 rs1353542169	365	S>N		No	ClinGen gnomAD
CA372273805 rs1390535508	365	S>R		No	ClinGen gnomAD
rs1337409497 CA372273809	366	D>G		No	ClinGen gnomAD
rs939499874 CA372273806	366	D>N		No	ClinGen gnomAD
rs939499874 CA185506023	366	D>Y		No	ClinGen gnomAD
rs752894629 CA4874695	368	S>G		No	ClinGen ExAC TOPMed gnomAD
CA185506024 rs992582240	370	F>S		No	ClinGen TOPMed gnomAD
rs1353168487 CA372273859	373	C>S		No	ClinGen TOPMed

No associated diseases with Q96RU8

8 regional properties for Q96RU8

Type	Name	Position	InterPro Accession
domain	Protein kinase domain	489 - 774	IPR000719
domain	EGF-like domain	273 - 309	IPR000742
domain	S-locus glycoprotein domain	200 - 306	IPR000858
domain	Serine-threonine/tyrosine-protein kinase, catalytic domain	491 - 758	IPR001245
domain	Bulb-type lectin domain	18 - 168	IPR001480
domain	PAN/Apple domain	328 - 410	IPR003609
active_site	Serine/threonine-protein kinase, active site	610 - 622	IPR008271
domain	S-locus receptor kinase, C-terminal	761 - 802	IPR021820

Functions

		Description
EC Number
Subcellular Localization
PANTHER Family	PTHR22961	SER/THR PROTEIN KINASE-TRB
PANTHER Subfamily	PTHR22961:SF17	TRIBBLES HOMOLOG 1
PANTHER Protein Class	non-receptor serine/threonine protein kinase protein modifying enzyme
PANTHER Pathway Category	No pathway information available

3 GO annotations of cellular component

Name	Definition
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name	Definition
mitogen-activated protein kinase kinase binding	Binding to a mitogen-activated protein kinase kinase, a protein that can phosphorylate a MAP kinase.
protein kinase inhibitor activity	Binds to and stops, prevents or reduces the activity of a protein kinase, an enzyme which phosphorylates a protein.
RNA polymerase II-specific DNA-binding transcription factor binding	Binding to a sequence-specific DNA binding RNA polymerase II transcription factor, any of the factors that interact selectively and non-covalently with a specific DNA sequence in order to modulate transcription.
ubiquitin protein ligase binding	Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.
ubiquitin-protein transferase regulator activity	Binds to and modulates the activity of a ubiquitin-protein transferase, an enzyme that catalyzes the covalent attachment of ubiquitin to lysine in a substrate protein.

12 GO annotations of biological process

Name	Definition
JNK cascade	An intracellular protein kinase cascade containing at least a JNK (a MAPK), a JNKK (a MAPKK) and a JUN3K (a MAP3K). The cascade can also contain an additional tier: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinases in the downstream tier to transmit a signal within a cell.
negative regulation of DNA-binding transcription factor activity	Any process that stops, prevents, or reduces the frequency, rate or extent of the activity of a transcription factor, any factor involved in the initiation or regulation of transcription.
negative regulation of lipopolysaccharide-mediated signaling pathway	Any process that stops, prevents, or reduces the frequency, rate or extent of signaling in response to detection of lipopolysaccharide.
negative regulation of neutrophil differentiation	Any process that stops, prevents, or reduces the frequency, rate or extent of neutrophil differentiation.
negative regulation of protein kinase activity	Any process that stops, prevents, or reduces the frequency, rate or extent of protein kinase activity.
negative regulation of smooth muscle cell migration	Any process that stops, prevents, or reduces the frequency, rate or extent of smooth muscle cell migration.
negative regulation of smooth muscle cell proliferation	Any process that stops, prevents or reduces the rate or extent of smooth muscle cell proliferation.
positive regulation of eosinophil differentiation	Any process that activates or increases the frequency, rate or extent of eosinophil differentiation.
positive regulation of macrophage differentiation	Any process that activates or increases the frequency, rate or extent of macrophage differentiation.
positive regulation of proteasomal ubiquitin-dependent protein catabolic process	Any process that activates or increases the frequency, rate or extent of the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome.
regulation of MAP kinase activity	Any process that modulates the frequency, rate or extent of MAP kinase activity.
response to lipopolysaccharide	Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria.

65 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q02066		Abscisic acid-inducible protein kinase	Triticum aestivum (Wheat)	PR
Q3SZW1	TSSK1B	Testis-specific serine/threonine-protein kinase 1	Bos taurus (Bovine)	PR
Q0VCE3	TRIB3	Tribbles homolog 3	Bos taurus (Bovine)	SS
Q5GLH2	TRIB2	Tribbles homolog 2	Bos taurus (Bovine)	SS
Q28283	TRIB2	Tribbles homolog 2	Canis lupus familiaris (Dog) (Canis familiaris)	SS
Q14680	MELK	Maternal embryonic leucine zipper kinase	Homo sapiens (Human)	EV
Q15831	STK11	Serine/threonine-protein kinase STK11	Homo sapiens (Human)	PR
Q13131	PRKAA1	5'-AMP-activated protein kinase catalytic subunit alpha-1	Homo sapiens (Human)	EV
Q96RG2	PASK	PAS domain-containing serine/threonine-protein kinase	Homo sapiens (Human)	EV
P54646	PRKAA2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Homo sapiens (Human)	EV
Q9BXA7	TSSK1B	Testis-specific serine/threonine-protein kinase 1	Homo sapiens (Human)	PR
Q96RU7	TRIB3	Tribbles homolog 3	Homo sapiens (Human)	PR
Q92519	TRIB2	Tribbles homolog 2	Homo sapiens (Human)	SS
Q8BRK8	Prkaa2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Mus musculus (Mouse)	SS
Q61241	Tssk1b	Testis-specific serine/threonine-protein kinase 1	Mus musculus (Mouse)	PR
Q61846	Melk	Maternal embryonic leucine zipper kinase	Mus musculus (Mouse)	PR
O54863	Tssk2	Testis-specific serine/threonine-protein kinase 2	Mus musculus (Mouse)	PR
Q5EG47	Prkaa1	5'-AMP-activated protein kinase catalytic subunit alpha-1	Mus musculus (Mouse)	SS
Q8K4K2	Trib3	Tribbles homolog 3	Mus musculus (Mouse)	SS
Q8K4K3	Trib2	Tribbles homolog 2	Mus musculus (Mouse)	SS
Q8K4K4	Trib1	Tribbles homolog 1	Mus musculus (Mouse)	SS
Q28948	PRKAA2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Sus scrofa (Pig)	SS
Q09137	Prkaa2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Rattus norvegicus (Rat)	EV
P54645	Prkaa1	5'-AMP-activated protein kinase catalytic subunit alpha-1	Rattus norvegicus (Rat)	EV
Q9WTQ6	Trib3	Tribbles homolog 3	Rattus norvegicus (Rat)	SS
Q5QNM6	CIPK13	Putative CBL-interacting protein kinase 13	Oryza sativa subsp japonica (Rice)	PR
Q5JLQ9	CIPK30	CBL-interacting protein kinase 30	Oryza sativa subsp japonica (Rice)	PR
Q5N942	SAPK4	Serine/threonine-protein kinase SAPK4	Oryza sativa subsp japonica (Rice)	PR
Q852Q0	OSK3	Serine/threonine protein kinase OSK3	Oryza sativa subsp. japonica (Rice)	SS
Q75LR7	SAPK1	Serine/threonine-protein kinase SAPK1	Oryza sativa subsp japonica (Rice)	PR
Q75H77	SAPK10	Serine/threonine-protein kinase SAPK10	Oryza sativa subsp japonica (Rice)	PR
Q7Y0B9	SAPK8	Serine/threonine-protein kinase SAPK8	Oryza sativa subsp japonica (Rice)	PR
Q7XQP4	SAPK7	Serine/threonine-protein kinase SAPK7	Oryza sativa subsp japonica (Rice)	PR
Q852Q2	OSK1	Serine/threonine protein kinase OSK1	Oryza sativa subsp. japonica (Rice)	SS
Q6ZLP5	CIPK23	CBL-interacting protein kinase 23	Oryza sativa subsp japonica (Rice)	PR
Q0D4J7	SAPK2	Serine/threonine-protein kinase SAPK2	Oryza sativa subsp japonica (Rice)	PR
Q8LIG4	CIPK3	CBL-interacting protein kinase 3	Oryza sativa subsp japonica (Rice)	PR
Q852Q1	OSK4	Serine/threonine protein kinase OSK4	Oryza sativa subsp. japonica (Rice)	SS
Q6ERS4	CIPK16	CBL-interacting protein kinase 16	Oryza sativa subsp japonica (Rice)	PR
P0C5D6	SAPK3	Serine/threonine-protein kinase SAPK3	Oryza sativa subsp japonica (Rice)	PR
Q2RAX3	CIPK33	CBL-interacting protein kinase 33	Oryza sativa subsp japonica (Rice)	PR
Q75V57	SAPK9	Serine/threonine-protein kinase SAPK9	Oryza sativa subsp japonica (Rice)	PR
Q21017	kin-29	Serine/threonine-protein kinase kin-29	Caenorhabditis elegans	SS
P45894	aak-1	5'-AMP-activated protein kinase catalytic subunit alpha-1	Caenorhabditis elegans	SS
Q95ZQ4	aak-2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Caenorhabditis elegans	SS
G5EED4	nipi-3	Protein nipi-3	Caenorhabditis elegans	PR
P43291	SRK2A	Serine/threonine-protein kinase SRK2A	Arabidopsis thaliana (Mouse-ear cress)	PR
Q93VD3	CIPK23	CBL-interacting serine/threonine-protein kinase 23	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9C958	SRK2B	Serine/threonine-protein kinase SRK2B	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9M9E9	SRK2C	Serine/threonine-protein kinase SRK2C	Arabidopsis thaliana (Mouse-ear cress)	PR
O64812	SRK2J	Serine/threonine-protein kinase SRK2J	Arabidopsis thaliana (Mouse-ear cress)	PR
Q2V452	CIPK3	CBL-interacting serine/threonine-protein kinase 3	Arabidopsis thaliana (Mouse-ear cress)	SS
O22971	CIPK13	CBL-interacting serine/threonine-protein kinase 13	Arabidopsis thaliana (Mouse-ear cress)	PR
Q38997	KIN10	SNF1-related protein kinase catalytic subunit alpha KIN10	Arabidopsis thaliana (Mouse-ear cress)	SS
P92958	KIN11	SNF1-related protein kinase catalytic subunit alpha KIN11	Arabidopsis thaliana (Mouse-ear cress)	PR
Q39192	SRK2D	Serine/threonine-protein kinase SRK2D	Arabidopsis thaliana (Mouse-ear cress)	PR
O65554	CIPK6	CBL-interacting serine/threonine-protein kinase 6	Arabidopsis thaliana (Mouse-ear cress)	PR
Q940H6	SRK2E	Serine/threonine-protein kinase SRK2E	Arabidopsis thaliana (Mouse-ear cress)	PR
P43292	SRK2G	Serine/threonine-protein kinase SRK2G	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9LDI3	CIPK24	CBL-interacting serine/threonine-protein kinase 24	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9FLZ3	KIN12	SNF1-related protein kinase catalytic subunit alpha KIN12	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9FJ54	CIPK20	CBL-interacting serine/threonine-protein kinase 20	Arabidopsis thaliana (Mouse-ear cress)	PR
Q94CG0	CIPK21	CBL-interacting serine/threonine-protein kinase 21	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9FFP9	SRK2H	Serine/threonine-protein kinase SRK2H	Arabidopsis thaliana (Mouse-ear cress)	PR
Q39193	SRK2I	Serine/threonine-protein kinase SRK2I	Arabidopsis thaliana (Mouse-ear cress)	PR

10	20	30	40	50	60
MRVGPVRSAM	SGASQPRGPA	LLFPATRGVP	AKRLLDADDA	AAVAAKCPRL	SECSSPPDYL
70	80	90	100	110	120
SPPGSPCSPQ	PPPAAPGAGG	GSGSAPGPSR	IADYLLLPLA	EREHVSRALC	IHTGRELRCK
130	140	150	160	170	180
VFPIKHYQDK	IRPYIQLPSH	SNITGIVEVI	LGETKAYVFF	EKDFGDMHSY	VRSRKRLREE
190	200	210	220	230	240
EAARLFKQIV	SAVAHCHQSA	IVLGDLKLRK	FVFSTEERTQ	LRLESLEDTH	IMKGEDDALS
250	260	270	280	290	300
DKHGCPAYVS	PEILNTTGTY	SGKAADVWSL	GVMLYTLLVG	RYPFHDSDPS	ALFSKIRRGQ
310	320	330	340	350	360
FCIPEHISPK	ARCLIRSLLR	REPSERLTAP	EILLHPWFES	VLEPGYIDSE	IGTSDQIVPE
370
YQEDSDISSF	FC