AiPD: Autoinhibited Protein Database

Q96FZ7

Gene name	CHMP6 (VPS20)
Protein name	Charged multivesicular body protein 6
Names	Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20
Species	Homo sapiens (Human)
KEGG Pathway	hsa:79643
EC number
Protein Class

Descriptions

Charged multivesicular body protein 4b (Chmp4b) is a core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. In a study with human CHMP2A, removing 42 amino acids from the C-terminal of the protein unmasks a common ability to associate with endosomal membranes and assemble into large polymeric complexes, and the C-terminal sequences are also important for interaction of ESCRT-III protein with VPS4. Thus, ESCRT-III proteins cycle between a closed state and an activated open state is under control of sequences at the C-terminus and associated factors.

Autoinhibitory domains (AIDs)

168-201 (C-terminal autoinhibitory region) SS

Target domain	1-144 (N-terminal α1-α4 domains)
Relief mechanism	Partner binding
Assay

AID

168-201 (C-terminal autoinhibitory region)

Target domain

1-144 (N-terminal α1-α4 domains)

Relief mechanism

Partner binding (Binding of Vps4 to C-terminal α6 domain)

Assay

Accessory elements

No accessory elements

References

Shim S et al. (2007) "Structure/function analysis of four core ESCRT-III proteins reveals common regulatory role for extreme C-terminal domain", Traffic (Copenhagen, Denmark), 8, 1068-79

Autoinhibited structure

Activated structure

3 structures for Q96FZ7

Entry ID	Method	Resolution	Chain	Position	Source
2K3W	NMR	-	B	166-181	PDB
3HTU	X-ray	200 A	B/D/F/H	11-48	PDB
AF-Q96FZ7-F1	Predicted				AlphaFoldDB

166 variants for Q96FZ7

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs1168062579 CA401413658	4	L>Q		No	ClinGen gnomAD
CA401413668 rs1428130137	5	F>L		No	ClinGen TOPMed gnomAD
rs753720858 CA8825888	6	G>D		No	ClinGen ExAC gnomAD
CA401413676 rs1178176355	7	R>H		No	ClinGen TOPMed gnomAD
CA401413677 rs1178176355	7	R>P		No	ClinGen TOPMed gnomAD
rs866041940 CA294979601	7	R>S		No	ClinGen Ensembl
CA294979605 rs866935455	8	K>R		No	ClinGen Ensembl
CA401413714 rs1316115445	10	Q>R		No	ClinGen gnomAD
CA294979616 rs531055555	11	S>N		No	ClinGen 1000Genomes gnomAD
CA401413738 rs1278973196	12	R>G		No	ClinGen gnomAD
rs757131739 CA401413747	12	R>L		No	ClinGen ExAC gnomAD
rs757131739 CA8825890	12	R>P		No	ClinGen ExAC gnomAD
CA401413777 rs1215925486	15	E>K		No	ClinGen TOPMed gnomAD
CA294979625 rs549254852	18	K>R		No	ClinGen 1000Genomes TOPMed gnomAD
CA8825905 rs759794169	22	Q>H		No	ClinGen ExAC gnomAD
rs1418196604 CA401414018	23	L>M		No	ClinGen gnomAD
CA8825907 rs752854065	27	R>Q		No	ClinGen ExAC gnomAD
rs375030925 COSM1387178 CA294981387	27	R>W	large_intestine [Cosmic]	No	ClinGen cosmic curated ESP gnomAD
rs760760031 CA8825908	31	R>K		No	ClinGen ExAC TOPMed gnomAD
rs1391699315 CA401414084	32	Q>H		No	ClinGen gnomAD
rs1305079582 CA401414080	32	Q>P		No	ClinGen TOPMed gnomAD
CA401414100 rs1390997215	34	Q>H		No	ClinGen gnomAD
CA401414124 rs913344986	38	A>S		No	ClinGen TOPMed gnomAD
rs913344986 CA294981409 COSM1387179	38	A>T	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
CA401414135 rs1354158007	39	Q>H		No	ClinGen TOPMed
rs1241396666 CA401414141	40	Q>L		No	ClinGen TOPMed gnomAD
rs1328933018 CA401414147	41	L>P		No	ClinGen TOPMed gnomAD
rs1314960544 CA401414149	42	E>K		No	ClinGen TOPMed gnomAD
CA8825910 rs753912872	43	R>C		No	ClinGen ExAC TOPMed gnomAD
CA294981414 rs753912872	43	R>G		No	ClinGen ExAC TOPMed gnomAD
CA8825911 rs757260155	43	R>H		No	ClinGen ExAC TOPMed gnomAD
rs757260155 CA401414159	43	R>L		No	ClinGen ExAC TOPMed gnomAD
CA401414158 rs757260155	43	R>P		No	ClinGen ExAC TOPMed gnomAD
CA294981429 rs946123498	44	E>K		No	ClinGen TOPMed gnomAD
CA8825913 rs367935751	45	R>C		No	ClinGen ESP ExAC TOPMed gnomAD
CA8825914 rs370737009	45	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
rs768409056 CA8825917	46	A>D		No	ClinGen ExAC gnomAD
rs746599881 COSM1201075 CA8825916	46	A>T	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs1446425080 CA401414498	48	A>T		No	ClinGen gnomAD
CA8825919 rs747578129	49	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA8825918 rs375540118	49	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
rs774668805 CA8825921	53	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA8825924 rs143663975	54	D>E		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs572398921 CA8825922	54	D>N		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA401414574 rs1336541479	55	G>D		No	ClinGen gnomAD
CA8825925 RCV000957714 VAR_061807 rs61037507	55	G>S		No	ClinGen ClinVar UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA294981480 rs531696317	57	K>R		No	ClinGen 1000Genomes
CA8825948 rs773327335	59	R>Q		No	ClinGen ExAC TOPMed gnomAD
rs545758125 CA8825947	59	R>W		No	ClinGen ExAC TOPMed gnomAD
rs762864286 CA8825949	60	A>T		No	ClinGen ExAC gnomAD
CA8825950 rs766385072	61	K>E		No	ClinGen ExAC gnomAD
CA401414709 rs1484880586	62	L>V		No	ClinGen gnomAD
rs751446580 CA8825951	63	L>P		No	ClinGen ExAC gnomAD
CA401414729 rs1277431773	64	L>H		No	ClinGen TOPMed
rs1478192729 CA401414741	65	K>R		No	ClinGen gnomAD
rs1396866207 CA401414750	66	K>E		No	ClinGen gnomAD
rs1198901998 CA401414765	67	K>Q		No	ClinGen gnomAD
rs1427533149 CA401414789	68	R>*		No	ClinGen gnomAD
rs202074825 CA8825954	68	R>Q		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA401414813 rs1345096674	70	Q>*		No	ClinGen gnomAD
CA401414815 rs1375422835	70	Q>R		No	ClinGen TOPMed
CA401414873 rs1393861415	74	L>P		No	ClinGen gnomAD
CA401414906 rs1340532521	77	T>A		No	ClinGen gnomAD
rs777217739 CA8825956	77	T>M		No	ClinGen ExAC TOPMed gnomAD
rs758954913 CA8825958	78	E>G		No	ClinGen ExAC TOPMed gnomAD
rs1219563645 CA401414933	79	N>D		No	ClinGen gnomAD
CA401414940 rs1283236782	79	N>T		No	ClinGen gnomAD
rs1314939752 CA401414995	83	S>C		No	ClinGen gnomAD
rs780491368 CA8825959	86	A>T		No	ClinGen ExAC TOPMed gnomAD
COSM1387180 CA8825960 rs146664368	87	M>T	large_intestine [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA401415043 rs1598439049	87	M>V		No	ClinGen Ensembl
rs1187324321 CA401415144	88	V>A		No	ClinGen TOPMed gnomAD
CA401415135 rs1329035184	88	V>I		No	ClinGen TOPMed
rs771064246 CA8825984	90	S>N		No	ClinGen ExAC TOPMed gnomAD
rs774563156 CA8825985	92	E>D		No	ClinGen ExAC gnomAD
CA401415253 rs148861093	96	I>M		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs759617929 CA8825986	96	I>V		No	ClinGen ExAC gnomAD
COSM3712596 rs775200526 CA8825988	97	E>K	upper_aerodigestive_tract [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs1168163552 CA401415294	99	K>R		No	ClinGen gnomAD
CA294982133 rs960947086	100	V>M		No	ClinGen Ensembl
rs763687939 CA8825990	101	M>V		No	ClinGen ExAC gnomAD
rs770316794 CA294982152	105	Q>R		No	ClinGen TOPMed gnomAD
CA294982158 rs969096884	106	F>S		No	ClinGen TOPMed
rs899231371 CA401415424	108	N>I		No	ClinGen TOPMed gnomAD
CA294982166 rs899231371	108	N>S		No	ClinGen TOPMed gnomAD
CA401415450 rs1204509310	110	C>S		No	ClinGen TOPMed
rs1324363608 CA401415477	112	N>K		No	ClinGen gnomAD
CA8825994 rs749930438	113	K>E		No	ClinGen ExAC gnomAD
CA401415504 rs1450204192	114	M>I		No	ClinGen gnomAD
CA294982173 rs1026333818	114	M>T		No	ClinGen TOPMed
CA294982171 rs993527464	114	M>V		No	ClinGen TOPMed
CA8825996 rs781707570	115	H>Q		No	ClinGen ExAC TOPMed gnomAD
CA8825995 rs755517578	115	H>Y		No	ClinGen ExAC TOPMed gnomAD
rs772807455 CA8826032	117	V>G		No	ClinGen ExAC gnomAD
rs1290343604 CA401415976	117	V>M		No	ClinGen gnomAD
rs1437011435 CA401415993	118	M>I		No	ClinGen gnomAD
rs762369762 CA8826033	118	M>V		No	ClinGen ExAC gnomAD
rs765854602 CA8826034	119	S>C		No	ClinGen ExAC gnomAD
CA8826036 rs761287804	120	I>F		No	ClinGen ExAC TOPMed gnomAD
rs764679129 CA8826037	120	I>T		No	ClinGen ExAC gnomAD
CA401416014 rs1598439912	121	E>K		No	ClinGen Ensembl
CA401416022 rs1384615521	121	E>V		No	ClinGen gnomAD
CA8826038 rs754281461	122	E>K		No	ClinGen ExAC TOPMed gnomAD
CA401416057 rs1598439921	125	R>G		No	ClinGen Ensembl
CA8826039 rs757660097	125	R>K		No	ClinGen ExAC gnomAD
CA8826041 rs750603328	126	I>T		No	ClinGen ExAC TOPMed gnomAD
rs979496082 CA294983251	128	D>N		No	ClinGen TOPMed
rs148035263 CA8826043	129	E>K		No	ClinGen ESP ExAC gnomAD
COSM985768 rs751560399 CA8826044	130	T>M	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA401416118 rs751560399	130	T>R		No	ClinGen ExAC TOPMed gnomAD
CA8826048 rs769508597	134	V>M		No	ClinGen ExAC TOPMed gnomAD
CA401416188 rs1338185676	136	Y>F		No	ClinGen TOPMed
rs1406323010 CA401416180	136	Y>H		No	ClinGen TOPMed
rs1338185676 CA401416185	136	Y>S		No	ClinGen TOPMed
CA8826049 rs777413976	137	Q>R		No	ClinGen ExAC gnomAD
rs571705472 CA8826051	138	R>Q		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA8826050 rs374165984	138	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
CA401416266 rs1467993438	140	I>T		No	ClinGen TOPMed gnomAD
rs749017305 CA8826070	140	I>V		No	ClinGen ExAC TOPMed gnomAD
CA8826073 rs778393768	142	E>K		No	ClinGen ExAC TOPMed gnomAD
CA8826072 rs778393768	142	E>Q		No	ClinGen ExAC TOPMed gnomAD
CA8826074 rs771651012	143	L>F		No	ClinGen ExAC TOPMed gnomAD
rs775129103 CA8826075	145	A>E		No	ClinGen ExAC gnomAD
rs775129103 CA401416312	145	A>V		No	ClinGen ExAC gnomAD
rs762478419 CA8826076	148	F>L		No	ClinGen ExAC gnomAD
CA8826078 rs370646614	149	T>S		No	ClinGen ESP ExAC TOPMed gnomAD
rs770391920 CA8826077	149	T>S		No	ClinGen ExAC gnomAD
CA8826080 rs763177718	151	E>G		No	ClinGen ExAC TOPMed gnomAD
CA8826079 rs763177718	151	E>V		No	ClinGen ExAC TOPMed gnomAD
rs1598440045 CA401416397	154	D>A		No	ClinGen Ensembl
rs751661361 CA401416399	154	D>E		No	ClinGen ExAC TOPMed gnomAD
rs759621441 CA401416406	155	A>S		No	ClinGen ExAC gnomAD
rs759621441 CA8826082	155	A>T		No	ClinGen ExAC gnomAD
CA8826083 rs767639574	156	I>V		No	ClinGen ExAC gnomAD
rs142871819 CA8826085	157	L>V		No	ClinGen ESP ExAC TOPMed gnomAD
CA8826086 rs763891085	158	E>K		No	ClinGen ExAC gnomAD
CA8826087 rs368586729	159	E>K		No	ClinGen ESP ExAC TOPMed gnomAD
CA401416477 rs1320711205	161	S>N		No	ClinGen TOPMed
rs199688363 CA8826088	162	A>T		No	ClinGen ExAC TOPMed gnomAD
rs778641560 CA8826089	162	A>V		No	ClinGen ExAC gnomAD
CA8826092 rs199755726	163	I>L		No	ClinGen ExAC TOPMed gnomAD
CA8826091 rs199755726	163	I>V		No	ClinGen ExAC TOPMed gnomAD
rs567503075 CA294984595	167	Q>E		No	ClinGen ExAC gnomAD
rs567503075 CA8826165	167	Q>K		No	ClinGen ExAC gnomAD
rs1568029539 CA401417008	168	I>T		No	ClinGen Ensembl
CA401417003 rs1403087858	168	I>V		No	ClinGen TOPMed
rs768892232 CA8826167	171	P>R		No	ClinGen ExAC TOPMed gnomAD
CA8826168 rs745597012	173	V>L		No	ClinGen ExAC TOPMed gnomAD
rs769881954 CA8826170	175	S>F		No	ClinGen ExAC TOPMed gnomAD
CA8826172 rs762854982	176	E>K		No	ClinGen ExAC TOPMed gnomAD
CA8826173 rs762854982	176	E>Q		No	ClinGen ExAC TOPMed gnomAD
rs751391493 CA8826174	178	L>F		No	ClinGen ExAC gnomAD
CA401417115 rs1442291974	179	P>R		No	ClinGen gnomAD
rs759148380 CA8826175	179	P>S		No	ClinGen ExAC gnomAD
CA401417123 rs1238662062	180	E>Q		No	ClinGen gnomAD
rs1021806302 CA294984725	183	P>L		No	ClinGen Ensembl
CA8826250 rs758387244	186	V>I		No	ClinGen ExAC TOPMed gnomAD
CA8826251 rs779819555	187	P>L		No	ClinGen ExAC gnomAD
CA401418243 rs1433900763	187	P>S		No	ClinGen TOPMed
CA8826252 rs149377306	190	A>D		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA8826253 rs768379998	193	R>K		No	ClinGen ExAC gnomAD
rs747860727 CA8826255	194	Q>H		No	ClinGen ExAC gnomAD
rs144716331 CA8826256	195	A>S		No	ClinGen ESP ExAC TOPMed gnomAD
rs374101426 COSM77470 CA8826257	195	A>V	ovary large_intestine [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
rs1598440835 CA401418314	198	V>G		No	ClinGen Ensembl
rs772663792 CA8826259	201	S>L		No	ClinGen ExAC gnomAD

No associated diseases with Q96FZ7

No regional properties for Q96FZ7

Type	Name	Position	InterPro Accession
No domain, repeats, and functional sites for Q96FZ7

Functions

		Description
EC Number
Subcellular Localization	Endomembrane system Endosome membrane; Lipid-anchor Late endosome membrane Membrane ; Lipid-anchor	Localizes to endosomal membranes
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

15 GO annotations of cellular component

Name	Definition
amphisome membrane	Any membrane that is part of an amphisome.
autophagosome membrane	The lipid bilayer surrounding an autophagosome, a double-membrane-bounded vesicle in which endogenous cellular material is sequestered.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
endosome membrane	The lipid bilayer surrounding an endosome.
ESCRT III complex	A complex with membrane scission activity that plays a major role in many processes where membranes are remodelled - including endosomal transport (vesicle budding), nuclear envelope organisation (membrane closure, mitotic bridge cleavage), and cytokinesis (abscission).
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
kinetochore	A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
kinetochore microtubule	Any of the spindle microtubules that attach to the kinetochores of chromosomes by their plus ends, and maneuver the chromosomes during mitotic or meiotic chromosome segregation.
lysosomal membrane	The lipid bilayer surrounding the lysosome and separating its contents from the cell cytoplasm.
membrane	A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
midbody	A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
multivesicular body	A type of endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.
multivesicular body membrane	The lipid bilayer surrounding a multivesicular body.
nuclear pore	A protein complex providing a discrete opening in the nuclear envelope of a eukaryotic cell, where the inner and outer nuclear membranes are joined.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

2 GO annotations of molecular function

Name	Definition
protein N-terminus binding	Binding to a protein N-terminus, the end of any peptide chain at which the 2-amino (or 2-imino) function of a constituent amino acid is not attached in peptide linkage to another amino-acid residue.
protein-containing complex binding	Binding to a macromolecular complex.

22 GO annotations of biological process

Name	Definition
autophagosome maturation	Removal of PI3P and Atg8/LC3 after the closure of the phagophore and before the fusion with the endosome/lysosome (e.g. mammals and insects) or vacuole (yeast), and that very likely destabilizes other Atg proteins and thus enables their efficient dissociation and recycling.
autophagy	The cellular catabolic process in which cells digest parts of their own cytoplasm; allows for both recycling of macromolecular constituents under conditions of cellular stress and remodeling the intracellular structure for cell differentiation.
ESCRT III complex assembly	The aggregation, arrangement and bonding together of a set of components to form an ESCRT III complex.
late endosome to lysosome transport	The directed movement of substances from late endosome to lysosome.
late endosome to vacuole transport via multivesicular body sorting pathway	The directed movement of substances from endosomes to vacuoles by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the vacuole.
macroautophagy	The major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane-bounded autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane-bounded structure. Autophagosomes then fuse with a lysosome (or vacuole) releasing single-membrane-bounded autophagic bodies that are then degraded within the lysosome (or vacuole). Some types of macroautophagy, e.g. pexophagy, mitophagy, involve selective targeting of the targets to be degraded.
midbody abscission	The process by which the midbody, the cytoplasmic bridge that connects the two prospective daughter cells, is severed at the end of mitotic cytokinesis, resulting in two separate daughter cells.
mitotic metaphase plate congression	The cell cycle process in which chromosomes are aligned at the metaphase plate, a plane halfway between the poles of the mitotic spindle, during mitosis.
multivesicular body assembly	The aggregation, arrangement and bonding together of a set of components to form a multivesicular body, a type of late endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.
multivesicular body sorting pathway	A vesicle-mediated transport process in which transmembrane proteins are ubiquitylated to facilitate their entry into luminal vesicles of multivesicular bodies (MVBs); upon subsequent fusion of MVBs with lysosomes or vacuoles, the cargo proteins are degraded.
negative regulation of cell death	Any process that decreases the rate or frequency of cell death. Cell death is the specific activation or halting of processes within a cell so that its vital functions markedly cease, rather than simply deteriorating gradually over time, which culminates in cell death.
negative regulation of epidermal growth factor-activated receptor activity	Any process that stops, prevents, or reduces the frequency, rate or extent of EGF-activated receptor activity.
nuclear membrane reassembly	The reformation of the nuclear membranes following their breakdown in the context of a normal process.
nucleus organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the nucleus.
plasma membrane repair	The resealing of a cell plasma membrane after cellular wounding due to, for instance, mechanical stress.
protein transport	The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of mitotic spindle assembly	Any process that modulates the frequency, rate or extent of mitotic spindle assembly.
regulation of protein catabolic process	Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the breakdown of a protein by the destruction of the native, active configuration, with or without the hydrolysis of peptide bonds.
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	The chemical reactions and pathways resulting in the breakdown of a protein or peptide covalently tagged with ubiquitin, via the multivesicular body (MVB) sorting pathway; ubiquitin-tagged proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation.
vesicle budding from membrane	The evagination of a membrane, resulting in formation of a vesicle.
viral budding from plasma membrane	A viral budding that starts with formation of a membrane curvature in the host plasma membrane.
viral budding via host ESCRT complex	Viral budding which uses a host ESCRT protein complex, or complexes, to mediate the budding process.

1 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q9BY43	CHMP4A	Charged multivesicular body protein 4a	Homo sapiens (Human)	SS

10	20	30	40	50	60
MGNLFGRKKQ	SRVTEQDKAI	LQLKQQRDKL	RQYQKRIAQQ	LERERALARQ	LLRDGRKERA
70	80	90	100	110	120
KLLLKKKRYQ	EQLLDRTENQ	ISSLEAMVQS	IEFTQIEMKV	MEGLQFGNEC	LNKMHQVMSI
130	140	150	160	170	180
EEVERILDET	QEAVEYQRQI	DELLAGSFTQ	EDEDAILEEL	SAITQEQIEL	PEVPSEPLPE
190	200
KIPENVPVKA	RPRQAELVAA	S