Q95ZQ4

SS
Gene name
aak-2 (T01C8.1)
Protein name
5'-AMP-activated protein kinase catalytic subunit alpha-2
Names
AMPK subunit alpha-2 , EC 2.7.11.1
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_T01C8.1
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

The AMP-activated protein kinase (AMPK) is characterized by its ability to bind to AMP, which enables it to adjust enzymatic activity by sensing the cellular energy status and maintain the balance between ATP production and consumption in eukaryotic cells. Autoinhibitory domain (AID) binding might constrain the mobility of helix αC, hence resulting in an autoinhibited KD-AID with much lower kinase activity than that of the kinase domain alone.

Autoinhibitory domains (AIDs)

356-422 (Autoinhibitory region) SS

Target domain

96-370 (Catalytic kinase domain)

Relief mechanism

Ligand binding, PTM

Assay

Accessory elements

227-249 (Activation loop from InterPro)

Target domain

87-339 (Protein kinase domain)

Relief mechanism

Assay

227-249 (Activation loop from InterPro)

Target domain

87-339 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q95ZQ4

Entry ID Method Resolution Chain Position Source
AF-Q95ZQ4-F1 Predicted AlphaFoldDB

No variants for Q95ZQ4

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q95ZQ4

No associated diseases with Q95ZQ4

5 regional properties for Q95ZQ4

Type Name Position InterPro Accession
domain Protein kinase domain 87 - 339 IPR000719
active_site Serine/threonine-protein kinase, active site 206 - 218 IPR008271
binding_site Protein kinase, ATP binding site 93 - 116 IPR017441
domain AMPK, C-terminal adenylate sensor domain 464 - 554 IPR032270
domain PRKAA1/2, autoinhibitory domain 359 - 406 IPR049020

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
axon The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
nucleotide-activated protein kinase complex A protein complex that possesses nucleotide-dependent protein kinase activity. The nucleotide can be AMP (in S. pombe and human) or ADP (in S. cerevisiae).
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name Definition
AMP-activated protein kinase activity Catalysis of the reaction
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

10 GO annotations of biological process

Name Definition
cellular response to glucose starvation Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of glucose.
determination of adult lifespan The pathways that regulate the duration of the adult phase of the life-cycle of an animal.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
negative regulation of protein secretion Any process that stops, prevents, or reduces the frequency, rate or extent of the controlled release of a protein from a cell.
negative regulation of TOR signaling Any process that stops, prevents, or reduces the frequency, rate or extent of TOR signaling.
nematode pharyngeal pumping The contraction and relaxation movements of the pharyngeal muscle that mediate feeding in nematodes.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
positive regulation of dauer larval development Any process that increases the rate, frequency, or extent of dauer larval development, the process whose specific outcome is the progression of the dauer larva over time, through the facultative diapause of the dauer (enduring) larval stage, with specialized traits adapted for dispersal and long-term survival, with elevated stress resistance and without feeding.
positive regulation of protein secretion Any process that activates or increases the frequency, rate or extent of the controlled release of a protein from a cell.
serotonin receptor signaling pathway The series of molecular signals generated as a consequence of a serotonin receptor binding to one of its physiological ligands.

67 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q02066 Abscisic acid-inducible protein kinase Triticum aestivum (Wheat) PR
P06782 SNF1 Carbon catabolite-derepressing protein kinase Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EV
Q5GLH2 TRIB2 Tribbles homolog 2 Bos taurus (Bovine) SS
Q0VCE3 TRIB3 Tribbles homolog 3 Bos taurus (Bovine) SS
Q3SZW1 TSSK1B Testis-specific serine/threonine-protein kinase 1 Bos taurus (Bovine) PR
Q92519 TRIB2 Tribbles homolog 2 Homo sapiens (Human) SS
Q96RU7 TRIB3 Tribbles homolog 3 Homo sapiens (Human) PR
Q14680 MELK Maternal embryonic leucine zipper kinase Homo sapiens (Human) EV
Q96RU8 TRIB1 Tribbles homolog 1 Homo sapiens (Human) EV
Q15831 STK11 Serine/threonine-protein kinase STK11 Homo sapiens (Human) PR
Q96RG2 PASK PAS domain-containing serine/threonine-protein kinase Homo sapiens (Human) EV
Q9BXA7 TSSK1B Testis-specific serine/threonine-protein kinase 1 Homo sapiens (Human) PR
P54646 PRKAA2 5'-AMP-activated protein kinase catalytic subunit alpha-2 Homo sapiens (Human) EV
Q13131 PRKAA1 5'-AMP-activated protein kinase catalytic subunit alpha-1 Homo sapiens (Human) EV
Q8K4K3 Trib2 Tribbles homolog 2 Mus musculus (Mouse) SS
Q61241 Tssk1b Testis-specific serine/threonine-protein kinase 1 Mus musculus (Mouse) PR
Q8K4K2 Trib3 Tribbles homolog 3 Mus musculus (Mouse) SS
Q61846 Melk Maternal embryonic leucine zipper kinase Mus musculus (Mouse) PR
O54863 Tssk2 Testis-specific serine/threonine-protein kinase 2 Mus musculus (Mouse) PR
Q8K4K4 Trib1 Tribbles homolog 1 Mus musculus (Mouse) SS
Q5EG47 Prkaa1 5'-AMP-activated protein kinase catalytic subunit alpha-1 Mus musculus (Mouse) SS
Q8BRK8 Prkaa2 5'-AMP-activated protein kinase catalytic subunit alpha-2 Mus musculus (Mouse) SS
Q28948 PRKAA2 5'-AMP-activated protein kinase catalytic subunit alpha-2 Sus scrofa (Pig) SS
Q09136 PRKAA1 5'-AMP-activated protein kinase catalytic subunit alpha-1 Sus scrofa (Pig) SS
Q9WTQ6 Trib3 Tribbles homolog 3 Rattus norvegicus (Rat) SS
P54645 Prkaa1 5'-AMP-activated protein kinase catalytic subunit alpha-1 Rattus norvegicus (Rat) EV
Q09137 Prkaa2 5'-AMP-activated protein kinase catalytic subunit alpha-2 Rattus norvegicus (Rat) EV
Q5QNM6 CIPK13 Putative CBL-interacting protein kinase 13 Oryza sativa subsp japonica (Rice) PR
Q5JLQ9 CIPK30 CBL-interacting protein kinase 30 Oryza sativa subsp japonica (Rice) PR
Q5N942 SAPK4 Serine/threonine-protein kinase SAPK4 Oryza sativa subsp japonica (Rice) PR
Q852Q0 OSK3 Serine/threonine protein kinase OSK3 Oryza sativa subsp. japonica (Rice) SS
Q75LR7 SAPK1 Serine/threonine-protein kinase SAPK1 Oryza sativa subsp japonica (Rice) PR
Q75H77 SAPK10 Serine/threonine-protein kinase SAPK10 Oryza sativa subsp japonica (Rice) PR
Q7Y0B9 SAPK8 Serine/threonine-protein kinase SAPK8 Oryza sativa subsp japonica (Rice) PR
Q7XQP4 SAPK7 Serine/threonine-protein kinase SAPK7 Oryza sativa subsp japonica (Rice) PR
Q0D4J7 SAPK2 Serine/threonine-protein kinase SAPK2 Oryza sativa subsp japonica (Rice) PR
Q6ERS4 CIPK16 CBL-interacting protein kinase 16 Oryza sativa subsp japonica (Rice) PR
P0C5D6 SAPK3 Serine/threonine-protein kinase SAPK3 Oryza sativa subsp japonica (Rice) PR
Q75V57 SAPK9 Serine/threonine-protein kinase SAPK9 Oryza sativa subsp japonica (Rice) PR
Q6ZLP5 CIPK23 CBL-interacting protein kinase 23 Oryza sativa subsp japonica (Rice) PR
Q852Q1 OSK4 Serine/threonine protein kinase OSK4 Oryza sativa subsp. japonica (Rice) SS
Q852Q2 OSK1 Serine/threonine protein kinase OSK1 Oryza sativa subsp. japonica (Rice) SS
Q8LIG4 CIPK3 CBL-interacting protein kinase 3 Oryza sativa subsp japonica (Rice) PR
Q2RAX3 CIPK33 CBL-interacting protein kinase 33 Oryza sativa subsp japonica (Rice) PR
Q2QY53 CIPK32 CBL-interacting protein kinase 32 Oryza sativa subsp japonica (Rice) PR
P45894 aak-1 5'-AMP-activated protein kinase catalytic subunit alpha-1 Caenorhabditis elegans SS
Q21017 kin-29 Serine/threonine-protein kinase kin-29 Caenorhabditis elegans SS
Q19469 sad-1 Serine/threonine kinase SAD-1 Caenorhabditis elegans SS
P43291 SRK2A Serine/threonine-protein kinase SRK2A Arabidopsis thaliana (Mouse-ear cress) PR
Q9C958 SRK2B Serine/threonine-protein kinase SRK2B Arabidopsis thaliana (Mouse-ear cress) PR
Q9M9E9 SRK2C Serine/threonine-protein kinase SRK2C Arabidopsis thaliana (Mouse-ear cress) PR
O64812 SRK2J Serine/threonine-protein kinase SRK2J Arabidopsis thaliana (Mouse-ear cress) PR
Q2V452 CIPK3 CBL-interacting serine/threonine-protein kinase 3 Arabidopsis thaliana (Mouse-ear cress) SS
O22971 CIPK13 CBL-interacting serine/threonine-protein kinase 13 Arabidopsis thaliana (Mouse-ear cress) PR
Q39192 SRK2D Serine/threonine-protein kinase SRK2D Arabidopsis thaliana (Mouse-ear cress) PR
O65554 CIPK6 CBL-interacting serine/threonine-protein kinase 6 Arabidopsis thaliana (Mouse-ear cress) PR
Q940H6 SRK2E Serine/threonine-protein kinase SRK2E Arabidopsis thaliana (Mouse-ear cress) PR
P43292 SRK2G Serine/threonine-protein kinase SRK2G Arabidopsis thaliana (Mouse-ear cress) PR
Q9FJ54 CIPK20 CBL-interacting serine/threonine-protein kinase 20 Arabidopsis thaliana (Mouse-ear cress) PR
Q9FFP9 SRK2H Serine/threonine-protein kinase SRK2H Arabidopsis thaliana (Mouse-ear cress) PR
Q39193 SRK2I Serine/threonine-protein kinase SRK2I Arabidopsis thaliana (Mouse-ear cress) PR
Q9FLZ3 KIN12 SNF1-related protein kinase catalytic subunit alpha KIN12 Arabidopsis thaliana (Mouse-ear cress) SS
Q93VD3 CIPK23 CBL-interacting serine/threonine-protein kinase 23 Arabidopsis thaliana (Mouse-ear cress) PR
Q94CG0 CIPK21 CBL-interacting serine/threonine-protein kinase 21 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LDI3 CIPK24 CBL-interacting serine/threonine-protein kinase 24 Arabidopsis thaliana (Mouse-ear cress) PR
P92958 KIN11 SNF1-related protein kinase catalytic subunit alpha KIN11 Arabidopsis thaliana (Mouse-ear cress) PR
Q38997 KIN10 SNF1-related protein kinase catalytic subunit alpha KIN10 Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MFSHQDRDRD RKEDGGGDGT EMKSKSRSQP SGLNRVKNLS RKLSAKSRKE RKDRDSTDNS
70 80 90 100 110 120
SKMSSPGGET STKQQQELKA QIKIGHYILK ETLGVGTFGK VKVGIHETTQ YKVAVKILNR
130 140 150 160 170 180
QKIKSLDVVG KIRREIQNLS LFRHPHIIRL YQVISTPSDI FMIMEHVSGG ELFDYIVKHG
190 200 210 220 230 240
RLKTAEARRF FQQIISGVDY CHRHMVVHRD LKPENLLLDE QNNVKIADFG LSNIMTDGDF
250 260 270 280 290 300
LRTSCGSPNY AAPEVISGKL YAGPEVDVWS CGVILYALLC GTLPFDDEHV PSLFRKIKSG
310 320 330 340 350 360
VFPTPDFLER PIVNLLHHML CVDPMKRATI KDVIAHEWFQ KDLPNYLFPP INESEASIVD
370 380 390 400 410 420
IEAVREVTEF QRYHVAEEEV TSALLGDDPH HHLSIAYNLI VDNKRIADET AKLSIEEFYQ
430 440 450 460 470 480
VTPNKGPGPV HRHPERIAAS VSSKITPTLD NTEASGANRN KRAKWHLGIR SQSRPEDIMF
490 500 510 520 530 540
EVFRAMKQLD MEWKVLNPYH VIVRRKPDAP AADPPKMSLQ LYQVDQRSYL LDFKSLADEE
550 560 570 580 590 600
SGSASASSSR HASMSMPQKP AGIRGTRTSS MPQAMSMEAS IEKMEVHDFS DMSCDVTPPP
610 620
SPGGAKLSQT MQFFEICAAL IGTLAR