Q95UN8
Gene name |
slpr (Mlk2, CG2272) |
Protein name |
Mitogen-activated protein kinase kinase kinase |
Names |
Mixed lineage kinase, Protein slipper, dMLK |
Species |
Drosophila melanogaster (Fruit fly) |
KEGG Pathway |
dme:Dmel_CG2272 |
EC number |
2.7.11.25: Protein-serine/threonine kinases |
Protein Class |
SERINE-THREONINE PROTEIN KINASE (PTHR23257) |
Descriptions
Drosophila Slpr, a mixed-lineage kinase (MLK), is required for Jun-N-terminal kinase (JNK) signaling. The N-terminal half of Slpr is involved in regulated signaling whereas the C-terminal half promotes cortical protein localization. The SH3 domain negatively regulates Slpr activity consistent with autoinhibition via a conserved proline motif. Slpr activation requires dual input from the MAP4K Misshapen (Msn) and the GTPase Rac, which both bind to the LZ-CRIB region of Slpr.
Autoinhibitory domains (AIDs)
Target domain |
485-487 (PVP motif within the LZ-CRIB linker) |
Relief mechanism |
Partner binding |
Assay |
Deletion assay, Mutagenesis experiment |
Accessory elements
289-310 (Activation loop from InterPro)
Target domain |
485-487 (PVP motif within the LZ-CRIB linker) |
Relief mechanism |
|
Assay |
|
Autoinhibited structure
Activated structure
1 structures for Q95UN8
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q95UN8-F1 | Predicted | AlphaFoldDB |
No variants for Q95UN8
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q95UN8 | |||||
No associated diseases with Q95UN8
No regional properties for Q95UN8
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| No domain, repeats, and functional sites for Q95UN8 | |||
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.25 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | PTHR23257 | SERINE-THREONINE PROTEIN KINASE |
| PANTHER Subfamily | PTHR23257:SF947 | PROTEIN KINASE DOMAIN-CONTAINING PROTEIN |
| PANTHER Protein Class |
non-receptor serine/threonine protein kinase
protein modifying enzyme |
|
| PANTHER Pathway Category | No pathway information available | |
1 GO annotations of cellular component
| Name | Definition |
|---|---|
| cell cortex | The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| JUN kinase kinase kinase activity | Catalysis of the reaction |
| MAP kinase kinase kinase activity | Catalysis of the phosphorylation and activation of a MAP kinase kinase; each MAP kinase kinase can be phosphorylated by any of several MAP kinase kinase kinases. |
| protein homodimerization activity | Binding to an identical protein to form a homodimer. |
| protein serine kinase activity | Catalysis of the reactions |
13 GO annotations of biological process
| Name | Definition |
|---|---|
| cellular response to ethanol | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ethanol stimulus. |
| dorsal appendage formation | Establishment of the dorsal filaments, elaborate specializations of the chorion that protrude from the anterior end of the egg and facilitate embryonic respiration. |
| dorsal closure | The process during Drosophila embryogenesis whereby the ectodermal cells of the lateral epithelium stretch in a coordinated fashion to internalize the amnioserosa cells and close the embryo dorsally. |
| dorsal closure, elongation of leading edge cells | The change in shape of cells at the dorsal-most (leading) edge of the epidermis from being polygonal to being elongated in the dorsal/ventral axis. |
| imaginal disc fusion, thorax closure | The joining of the parts of the wing imaginal discs, giving rise to the adult thorax. |
| imaginal disc-derived female genitalia morphogenesis | The process in which the anatomical structures of female genitalia are generated and organized from the genital disc. |
| imaginal disc-derived male genitalia morphogenesis | The process in which the anatomical structures of male genitalia are generated and organized from the genital imaginal disc. |
| JNK cascade | An intracellular protein kinase cascade containing at least a JNK (a MAPK), a JNKK (a MAPKK) and a JUN3K (a MAP3K). The cascade can also contain an additional tier |
| positive regulation of JNK cascade | Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the JNK cascade. |
| protein autophosphorylation | The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation). |
| protein phosphorylation | The process of introducing a phosphate group on to a protein. |
| response to heat | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism. |
| vascular endothelial growth factor receptor signaling pathway | The series of molecular signals initiated by a ligand binding to a vascular endothelial growth factor receptor (VEGFR) on the surface of the target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. |
39 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| A2VDU3 | MAP3K7 | Mitogen-activated protein kinase kinase kinase 7 | Bos taurus (Bovine) | SS |
| Q4TVR5 | DSTYK | Dual serine/threonine and tyrosine protein kinase | Bos taurus (Bovine) | PR |
| Q3SZJ2 | RIPK2 | Receptor-interacting serine/threonine-protein kinase 2 | Bos taurus (Bovine) | PR |
| Q6XUX0 | DSTYK | Dual serine/threonine and tyrosine protein kinase | Gallus gallus (Chicken) | PR |
| P83104 | Takl1 | Putative mitogen-activated protein kinase kinase kinase 7-like | Drosophila melanogaster (Fruit fly) | PR |
| P11346 | Raf | Raf homolog serine/threonine-protein kinase Raf | Drosophila melanogaster (Fruit fly) | PR |
| Q8NB16 | MLKL | Mixed lineage kinase domain-like protein | Homo sapiens (Human) | EV |
| P00540 | MOS | Proto-oncogene serine/threonine-protein kinase mos | Homo sapiens (Human) | PR |
| Q5TCX8 | MAP3K21 | Mitogen-activated protein kinase kinase kinase 21 | Homo sapiens (Human) | PR |
| Q6XUX3 | DSTYK | Dual serine/threonine and tyrosine protein kinase | Homo sapiens (Human) | PR |
| O43318 | MAP3K7 | Mitogen-activated protein kinase kinase kinase 7 | Homo sapiens (Human) | SS |
| Q9NYL2 | MAP3K20 | Mitogen-activated protein kinase kinase kinase 20 | Homo sapiens (Human) | PR |
| Q38SD2 | LRRK1 | Leucine-rich repeat serine/threonine-protein kinase 1 | Homo sapiens (Human) | EV |
| O43353 | RIPK2 | Receptor-interacting serine/threonine-protein kinase 2 | Homo sapiens (Human) | PR |
| P80192 | MAP3K9 | Mitogen-activated protein kinase kinase kinase 9 | Homo sapiens (Human) | SS |
| Q02779 | MAP3K10 | Mitogen-activated protein kinase kinase kinase 10 | Homo sapiens (Human) | SS |
| Q16584 | MAP3K11 | Mitogen-activated protein kinase kinase kinase 11 | Homo sapiens (Human) | EV |
| Q62073 | Map3k7 | Mitogen-activated protein kinase kinase kinase 7 | Mus musculus (Mouse) | EV |
| Q9D2Y4 | Mlkl | Mixed lineage kinase domain-like protein | Mus musculus (Mouse) | SS |
| Q9ESL4 | Map3k20 | Mitogen-activated protein kinase kinase kinase 20 | Mus musculus (Mouse) | PR |
| P00536 | Mos | Proto-oncogene serine/threonine-protein kinase mos | Mus musculus (Mouse) | PR |
| P58801 | Ripk2 | Receptor-interacting serine/threonine-protein kinase 2 | Mus musculus (Mouse) | PR |
| Q3U1V8 | Map3k9 | Mitogen-activated protein kinase kinase kinase 9 | Mus musculus (Mouse) | SS |
| Q66L42 | Map3k10 | Mitogen-activated protein kinase kinase kinase 10 | Mus musculus (Mouse) | SS |
| Q80XI6 | Map3k11 | Mitogen-activated protein kinase kinase kinase 11 | Mus musculus (Mouse) | PR |
| Q8VDG6 | Map3k21 | Mitogen-activated protein kinase kinase kinase 21 | Mus musculus (Mouse) | PR |
| P0C8E4 | Map3k7 | Mitogen-activated protein kinase kinase kinase 7 | Rattus norvegicus (Rat) | SS |
| P00539 | Mos | Proto-oncogene serine/threonine-protein kinase mos | Rattus norvegicus (Rat) | PR |
| D3ZG83 | Map3k10 | Mitogen-activated protein kinase kinase kinase 10 | Rattus norvegicus (Rat) | SS |
| Q66HA1 | Map3k11 | Mitogen-activated protein kinase kinase kinase 11 | Rattus norvegicus (Rat) | PR |
| Q9TZM3 | lrk-1 | Leucine-rich repeat serine/threonine-protein kinase 1 | Caenorhabditis elegans | SS |
| Q9FPR3 | EDR1 | Serine/threonine-protein kinase EDR1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q2MHE4 | HT1 | Serine/threonine/tyrosine-protein kinase HT1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| O22558 | STY8 | Serine/threonine-protein kinase STY8 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q8RWL6 | STY17 | Serine/threonine-protein kinase STY17 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| F4JTP5 | STY46 | Serine/threonine-protein kinase STY46 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q05609 | CTR1 | Serine/threonine-protein kinase CTR1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| O22042 | ANP3 | Mitogen-activated protein kinase kinase kinase 3 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q67E00 | dstyk | Dual serine/threonine and tyrosine protein kinase | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MPSQVSRGLD | TTNMLPISEE | QQLQQQQQQQ | QLEQLHHPQI | PEIPIPDLEQ | VETQVGDGSL |
| 70 | 80 | 90 | 100 | 110 | 120 |
| WTALYDYDAQ | GEDELTLRRG | EIVVVLSTDS | EVSGDVGWWT | GKIGDKVGVF | PKDFVTDEDP |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LQLNVSSAIG | DIQPHEIEYN | ELDIKEVIGS | GGFCKVHRGY | YDGEEVAIKI | AHQTGEDDMQ |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RMRDNVLQEA | KLFWALKHEN | IAALRGVCLN | TKLCLVMEYA | RGGSLNRILA | GKIPPDVLVN |
| 250 | 260 | 270 | 280 | 290 | 300 |
| WAIQIARGMN | YLHNEAPMSI | IHRDLKSSNV | LIYEAIEGNH | LQQKTLKITD | FGLAREMYNT |
| 310 | 320 | 330 | 340 | 350 | 360 |
| QRMSAAGTYA | WMPPEVISVS | TYSKFSDVWS | YGVLLWELIT | GETPYKGFDP | LSVAYGVAVN |
| 370 | 380 | 390 | 400 | 410 | 420 |
| TLTLPIPKTC | PETWGALMKS | CWQTDPHKRP | GFKEILKQLE | SIACSKFTLT | PQESFHYMQE |
| 430 | 440 | 450 | 460 | 470 | 480 |
| CWRKEIAGVL | HDLREKEKEL | RNKEEQLLRV | QNEQREKANL | LKIREQNLRE | RERVLIEREL |
| 490 | 500 | 510 | 520 | 530 | 540 |
| VMLQPVPSKR | KHKKGKKNKP | LQISLPTGFR | HTITAVRDKA | EQPGSPSFSG | LRIVALTDGH |
| 550 | 560 | 570 | 580 | 590 | 600 |
| KGKTWGPSTM | HQRERSLLPS | QLSGGQPEWP | AQTSTHSSFS | KSAPNLDKKQ | QQQNQQQVAS |
| 610 | 620 | 630 | 640 | 650 | 660 |
| LTPPPGLGIL | GGSGGAGGTP | ATPLLYPGIP | IILTRPNNNN | IGNCKAITTT | ITTTTTTTTN |
| 670 | 680 | 690 | 700 | 710 | 720 |
| NNNNNNNSIS | ANNNNQLNNI | STINSNNNNN | QTNLTSQPNT | IIVLQNGRNN | SNSSTTSQSP |
| 730 | 740 | 750 | 760 | 770 | 780 |
| AKIYHRARSQ | EYGLDHPLAY | QPPPLYLVTD | DSSETDTVAS | PTGCFHFLKS | GNSSAASGAV |
| 790 | 800 | 810 | 820 | 830 | 840 |
| HLHRFGGSLG | NSPAVGRKKH | SLDSSSHHPP | ANGSNSFALP | NQLTLPSEDN | NTYDHAFYRD |
| 850 | 860 | 870 | 880 | 890 | 900 |
| VIKKMSMASS | ERVNSKSSGD | LTMYNSSTPL | TARDCDDAEE | AFEGGRFQRN | FSGSQFPRHC |
| 910 | 920 | 930 | 940 | 950 | 960 |
| FFTRQEEEGE | AEDEDAVAAE | VDTADADADD | ECQVPASQMR | QNSTTSRKSS | VTFQSVSFEE |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| PDFVATPRTT | ARSDLYTSSA | SISFATYRSA | SPSLSSSSTT | ASASPSIAST | EAVNGYHMQE |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| NSILNTRRMQ | DVQPHPDVIK | LRAQEQRQQT | KNQKKQRPKH | ITKSKSVEAP | VEGQHHEHDD |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| HNDPQHQHHS | AGSSKIRALF | NLFTRSRKKY | SKLAEHNMVG | GPEFCAIDPY | QTDLAMGGSS |
| 1150 | 1160 | ||||
| RSLKRKGKKP | QTQSCEQLER | C |