Q95M30

SS
Gene name
HCK
Protein name
Tyrosine-protein kinase HCK
Names
Hematopoietic cell kinase, Hemopoietic cell kinase, p56-HCK
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
KEGG Pathway
mcf:102115729
EC number
2.7.10.2: Protein-tyrosine kinases
Protein Class

Descriptions

Tyrosine-protein kinase Hck is a member of the Src family kinases, and the control of Hck activity is essential for normal cellular function. It contains modular SH3 and SH2 domains, a regulatory linker (SH2-CD linker), the catalytic or kinase domain (SH1 domain), and a C-terminal negative regulatory tail. The SH3 and SH2-CD linker domains work together to downregulate kinase activity by engaging their intramolecular ligands (SH2 binds to the linker and SH2 binds to the tail). Kinase activity is also regulated by phosphorylation at regulatory tyrosine residues in C-terminal regulatory tail.

Autoinhibitory domains (AIDs)

56-116 (SH3 domain) SS

Target domain

240-493 (Protein kinase domain)

Relief mechanism

Partner binding, Ligand binding

Assay

122-219 (SH2 domain) SS

Target domain

240-493 (Protein kinase domain)

Relief mechanism

PTM

Assay

220-239 (SH2-CD linker) SS

Target domain

240-493 (Protein kinase domain)

Relief mechanism

Ligand binding

Assay

494-504 (C-terminal regulatory tail) SS

Target domain

240-493 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q95M30

Entry ID Method Resolution Chain Position Source
AF-Q95M30-F1 Predicted AlphaFoldDB

No variants for Q95M30

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q95M30

No associated diseases with Q95M30

8 regional properties for Q95M30

Type Name Position InterPro Accession
domain C2 domain 662 - 790 IPR000008
domain Phosphatidylinositol-specific phospholipase C, X domain 312 - 464 IPR000909
domain Phospholipase C, phosphatidylinositol-specific, Y domain 546 - 662 IPR001711
domain Phospholipase C-beta, C-terminal domain 979 - 1153 IPR014815
domain Phosphoinositide-specific phospholipase C, EF-hand-like domain 214 - 304 IPR015359
domain 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2, catalytic domain 311 - 649 IPR028403
domain PLC-beta, PH domain 12 - 144 IPR037862
domain 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2, EF-hand domain 149 - 299 IPR046969

Functions

Description
EC Number 2.7.10.2 Protein-tyrosine kinases
Subcellular Localization
  • Cytoplasmic vesicle, secretory vesicle
  • Cytoplasm, cytosol
  • Cell membrane; Lipid-anchor
  • Membrane, caveola ; Lipid-anchor
  • Cell junction, focal adhesion
  • Cytoplasm, cytoskeleton
  • Golgi apparatus
  • Cytoplasmic vesicle
  • Lysosome
  • Nucleus
  • A small fraction is associated with caveolae
  • Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3
  • Colocalizes with the actin cytoskeleton at focal adhesions (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

9 GO annotations of cellular component

Name Definition
caveola A membrane raft that forms small pit, depression, or invagination that communicates with the outside of a cell and extends inward, indenting the cytoplasm and the cell membrane. Examples include flask-shaped invaginations of the plasma membrane in adipocytes associated with caveolin proteins, and minute pits or incuppings of the cell membrane formed during pinocytosis. Caveolae may be pinched off to form free vesicles within the cytoplasm.
cytoskeleton A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
extrinsic component of cytoplasmic side of plasma membrane The component of a plasma membrane consisting of gene products and protein complexes that are loosely bound to its cytoplasmic surface, but not integrated into the hydrophobic region.
focal adhesion A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
lysosome A small lytic vacuole that has cell cycle-independent morphology found in most animal cells and that contains a variety of hydrolases, most of which have their maximal activities in the pH range 5-6. The contained enzymes display latency if properly isolated. About 40 different lysosomal hydrolases are known and lysosomes have a great variety of morphologies and functions.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
transport vesicle Any of the vesicles of the constitutive secretory pathway, which carry cargo from the endoplasmic reticulum to the Golgi, between Golgi cisternae, from the Golgi to the ER (retrograde transport) or to destinations within or outside the cell.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
non-membrane spanning protein tyrosine kinase activity Catalysis of the reaction: ATP + protein L-tyrosine = ADP + protein L-tyrosine phosphate by a non-membrane spanning protein.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.
protein tyrosine kinase activity Catalysis of the reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

11 GO annotations of biological process

Name Definition
exocytosis A process of secretion by a cell that results in the release of intracellular molecules (e.g. hormones, matrix proteins) contained within a membrane-bounded vesicle. Exocytosis can occur either by full fusion, when the vesicle collapses into the plasma membrane, or by a kiss-and-run mechanism that involves the formation of a transient contact, a pore, between a granule (for exemple of chromaffin cells) and the plasma membrane. The latter process most of the time leads to only partial secretion of the granule content. Exocytosis begins with steps that prepare vesicles for fusion with the membrane (tethering and docking) and ends when molecules are secreted from the cell.
inflammatory response The immediate defensive reaction (by vertebrate tissue) to infection or injury caused by chemical or physical agents. The process is characterized by local vasodilation, extravasation of plasma into intercellular spaces and accumulation of white blood cells and macrophages.
innate immune response Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens.
peptidyl-tyrosine phosphorylation The phosphorylation of peptidyl-tyrosine to form peptidyl-O4'-phospho-L-tyrosine.
phagocytosis A vesicle-mediated transport process that results in the engulfment of external particulate material by phagocytes and their delivery to the lysosome. The particles are initially contained within phagocytic vacuoles (phagosomes), which then fuse with primary lysosomes to effect digestion of the particles.
positive regulation of actin cytoskeleton reorganization Any process that activates or increases the frequency, rate or extent of actin cytoskeleton reorganization.
positive regulation of cell population proliferation Any process that activates or increases the rate or extent of cell proliferation.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
regulation of cell shape Any process that modulates the surface configuration of a cell.
regulation of phagocytosis Any process that modulates the frequency, rate or extent of phagocytosis, the process in which phagocytes engulf external particulate material.
regulation of podosome assembly Any process that modulates the frequency, rate or extent of podosome assembly.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MGCMKSKFLQ AGGNTFSKTE TSANPHCPVY VPDPTSTIKP GPNSNNRNTP GIGEGSEDII
70 80 90 100 110 120
VVALYDYEAI HHEDLSFQKG DQMVVLEESG EWWKARSLAT RKEGYIPSNY VARVDSLETE
130 140 150 160 170 180
EWFFKGISRK DAERQLLAPG NMLGSFMIRD SETTKGSYSL SVRDYDPRQG DTVKHYKIRT
190 200 210 220 230 240
LDNGGFYISP RSTFSTLQEL VDHYKKGSDG LCQKLSVPCV SSKPQKPWEK DAWEIPRESL
250 260 270 280 290 300
KLEKKLGAGQ FGEVWMATYN KHTKVAVKTM KPGSMSVEAF LAEANLMKTL QHDKLVKLHA
310 320 330 340 350 360
VVTKEPIYII TEFMAKGSLL DFLKSDEGSK QPLPKLIDFS AQIAEGMAFI EQRNYIHRDL
370 380 390 400 410 420
RAANILVSAS LVCKIADFGL ARIIEDNEYT AREGAKFPIK WTAPEAINFG SSTIKSDVWS
430 440 450 460 470 480
FGILLMEIVT YGRIPYPGMS NPEVIRALER GYRMPRPENC PEELYNIMMR CWKNRPEERP
490 500
TFEYIQSVLD DFYTATESQY QQQP