Q8VHJ5

SS
Gene name
Mark1 (Emk3, Kiaa1477)
Protein name
Serine/threonine-protein kinase MARK1
Names
EC 2.7.11.1 , EC 2.7.11.26 , ELKL motif serine/threonine-protein kinase 3 , MAP/microtubule affinity-regulating kinase 1 , PAR1 homolog c , Par-1c , mPar-1c
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:226778
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

MARK1 is a Ser/Thr protein kinase involved in various cellular processes. It is part of the CAMK-like kinase family and has roles in diseases like Alzheimer’s and cancer. The kinase’s autoinhibition is mediated by its C-terminal KA1 domain, which interacts with the kinase domain to prevent substrate binding and activation. The KA1 domain binds to the αD-helix, blocking the peptide substrate binding site and inhibiting the kinase. This autoinhibition is alleviated by the binding of anionic phospholipid membranes to the KA1 domain, which reverses MARK1 autoinhibition and stimulates kinase activity.

Autoinhibitory domains (AIDs)

695-795 (KA1 domain) SS

Target domain

60-311 (Protein kinase domain)

Relief mechanism

Others

Assay

Accessory elements

199-221 (Activation loop from InterPro)

Target domain

60-311 (Protein kinase domain)

Relief mechanism

Assay

199-221 (Activation loop from InterPro)

Target domain

60-311 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q8VHJ5

Entry ID Method Resolution Chain Position Source
AF-Q8VHJ5-F1 Predicted AlphaFoldDB

33 variants for Q8VHJ5

Variant ID(s) Position Change Description Diseaes Association Provenance
rs33826721 40 L>I No EVA
rs3388517671 65 T>R No EVA
rs3388517926 70 N>K No EVA
rs3388524945 109 R>Q No EVA
rs3388522282 245 V>A No EVA
rs3390996633 249 T>A No EVA
rs3388522269 265 L>H No EVA
rs3388522275 284 C>Y No EVA
rs3388521065 286 N>I No EVA
rs3388523810 301 S>I No EVA
rs48463844 318 E>D No EVA
rs3388519906 326 P>L No EVA
rs3388519841 335 R>T No EVA
rs3388524964 357 K>I No EVA
rs3388524290 364 T>I No EVA
rs3388522216 386 L>V No EVA
rs3388522236 426 A>T No EVA
rs3388517927 434 V>L No EVA
rs3388524993 455 K>* No EVA
rs3388524355 490 S>N No EVA
rs3388521596 493 V>A No EVA
rs211874876 515 A>T No EVA
rs3388524974 592 H>R No EVA
rs13476283 603 R>H No EVA
rs3390917788 633 S>P No EVA
rs3388524968 640 A>V No EVA
rs3388523983 647 G>C No EVA
rs234113235 674 A>T No EVA
rs3388521601 687 R>I No EVA
rs3388524959 704 W>* No EVA
rs3388524018 716 D>E No EVA
rs3388523425 752 L>H No EVA
rs3388522181 771 R>G No EVA

No associated diseases with Q8VHJ5

3 regional properties for Q8VHJ5

Type Name Position InterPro Accession
domain Protein kinase domain 199 - 527 IPR000719
active_site Serine/threonine-protein kinase, active site 320 - 332 IPR008271
binding_site Protein kinase, ATP binding site 205 - 228 IPR017441

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cell membrane ; Peripheral membrane protein
  • Cytoplasm, cytoskeleton
  • Cytoplasm
  • Cell projection, dendrite
  • Appears to localize to an intracellular network
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoskeleton A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

9 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
magnesium ion binding Binding to a magnesium (Mg) ion.
phosphatidic acid binding Binding to phosphatidic acid, any of a class of glycerol phosphate in which both the remaining hydroxyl groups of the glycerol moiety are esterified with fatty acids.
phosphatidylinositol-4,5-bisphosphate binding Binding to phosphatidylinositol-4,5-bisphosphate, a derivative of phosphatidylinositol in which the inositol ring is phosphorylated at the 4' and 5' positions.
phosphatidylserine binding Binding to phosphatidylserine, a class of glycophospholipids in which a phosphatidyl group is esterified to the hydroxyl group of L-serine.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions
tau-protein kinase activity Catalysis of the reaction

12 GO annotations of biological process

Name Definition
cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures.
establishment of mitochondrion localization The directed movement of the mitochondrion to a specific location.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
microtubule cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins.
negative regulation of epithelial to mesenchymal transition Any process that decreases the rate, frequency, or extent of epithelial to mesenchymal transition. Epithelial to mesenchymal transition where an epithelial cell loses apical/basolateral polarity, severs intercellular adhesive junctions, degrades basement membrane components and becomes a migratory mesenchymal cell.
negative regulation of gene expression Any process that decreases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
neuron migration The characteristic movement of an immature neuron from germinal zones to specific positions where they will reside as they mature.
positive regulation of gene expression Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of dendrite development Any process that modulates the frequency, rate or extent of dendrite development.
regulation of neuron projection development Any process that modulates the rate, frequency or extent of neuron projection development. Neuron projection development is the process whose specific outcome is the progression of a neuron projection over time, from its formation to the mature structure. A neuron projection is any process extending from a neural cell, such as axons or dendrites (collectively called neurites).
Wnt signaling pathway The series of molecular signals initiated by binding of a Wnt protein to a frizzled family receptor on the surface of the target cell and ending with a change in cell state.

20 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q96L34 MARK4 MAP/microtubule affinity-regulating kinase 4 Homo sapiens (Human) SS
P57059 SIK1 Serine/threonine-protein kinase SIK1 Homo sapiens (Human) PR
Q7KZI7 MARK2 Serine/threonine-protein kinase MARK2 Homo sapiens (Human) SS
P27448 MARK3 MAP/microtubule affinity-regulating kinase 3 Homo sapiens (Human) SS
Q9P0L2 MARK1 Serine/threonine-protein kinase MARK1 Homo sapiens (Human) EV
Q8C0N0 Gm4922 Sperm motility kinase Z Mus musculus (Mouse) PR
Q8C0X8 Sperm motility kinase X Mus musculus (Mouse) PR
A0AUV4 Gm7168 Sperm motility kinase Y Mus musculus (Mouse) PR
Q8VBY2 Camkk1 Calcium/calmodulin-dependent protein kinase kinase 1 Mus musculus (Mouse) PR
Q8CIP4 Mark4 MAP/microtubule affinity-regulating kinase 4 Mus musculus (Mouse) SS
Q05512 Mark2 Serine/threonine-protein kinase MARK2 Mus musculus (Mouse) SS
Q8CEE6 Pask PAS domain-containing serine/threonine-protein kinase Mus musculus (Mouse) SS
Q03141 Mark3 MAP/microtubule affinity-regulating kinase 3 Mus musculus (Mouse) SS
Q8VHF0 Mark3 MAP/microtubule affinity-regulating kinase 3 Rattus norvegicus (Rat) SS
O08679 Mark2 Serine/threonine-protein kinase MARK2 Rattus norvegicus (Rat) SS
O08678 Mark1 Serine/threonine-protein kinase MARK1 Rattus norvegicus (Rat) SS
Q852Q1 OSK4 Serine/threonine protein kinase OSK4 Oryza sativa subsp. japonica (Rice) SS
Q852Q2 OSK1 Serine/threonine protein kinase OSK1 Oryza sativa subsp. japonica (Rice) SS
Q852Q0 OSK3 Serine/threonine protein kinase OSK3 Oryza sativa subsp. japonica (Rice) SS
Q9TW45 par-1 Serine/threonine-protein kinase par-1 Caenorhabditis elegans SS
10 20 30 40 50 60
MSARTPLPTV NERDTENHTS VDGYTETHIP PAKSSSRQNL PRCRNSITSA TDEQPHIGNY
70 80 90 100 110 120
RLQKTIGKGN FAKVKLARHV LTGREVAVKI IDKTQLNPTS LQKLFREVRI MKILNHPNIV
130 140 150 160 170 180
KLFEVIETEK TLYLVMEYAS GGEVFDYLVA HGRMKEKEAR AKFRQIVSAV QYCHQKCIVH
190 200 210 220 230 240
RDLKAENLLL DADMNIKIAD FGFSNEFTVG NKLDTFCGSP PYAAPELFQG KKYDGPEVDV
250 260 270 280 290 300
WSLGVILYTL VSGSLPFDGQ NLKELRERVL RGKYRIPFYM STDCENLLKK LLVLNPIKRG
310 320 330 340 350 360
SLEQIMKDRW MNVGHEEEEL KPYSEPELDL SDAKRIDIMV TMGFARDEIN DALVSQKYDE
370 380 390 400 410 420
VMATYILLGR KPPEFEGGES LSSGSLCQRS RPSSDLNNST LQSPAHLKVQ RSISANQKQR
430 440 450 460 470 480
RFSDHAGPSI PPAVSYTKRP QANSVESEQK EEWGKDTARR LGSTTVGSKS EVTASPLVGP
490 500 510 520 530 540
DRKKSTASPS NNVYSGGSMA RRNTYVCERS TDRYAALQNG RDSSLTEMSA SSMSSAGSTV
550 560 570 580 590 600
ASAGPSARPR HQKSMSTSGH PIKVTLPTIK DGSEAYRPGA AQRVPAASPS AHSISASTPD
610 620 630 640 650 660
RTRFPRGSSS RSTFHGEQLR ERRSAAYNGP PASPSHETGA FAHARRGTST GIISKITSKF
670 680 690 700 710 720
VRRDPSEGEA SGRADTARGS SGDPKERDKD EGKEAKPRSL RFTWSMKTTS SMDPNDMLRE
730 740 750 760 770 780
IRKVLDANTC DYEQKERFLL FCVHGDARQD SLVQWEMEVC KLPRLSLNGV RFKRISGTSI
790
AFKNIASKIA NELKL