Q8VEJ9

PR
Gene name
Vps4a
Protein name
Vacuolar protein sorting-associated protein 4A
Names
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:116733
EC number
3.6.4.6: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

(Annotation based on sequence homology with Q9UN37)
VPS4 proteins are AAA+ ATPases required to form multivesicular bodies, release viral particles, and complete cytokinesis. VPS4 proteins act by disassembling ESCRT-III heteropolymers during or after their proposed function in membrane scission. Deleting the N-terminal MIT domain and adjacent linker from VPS4A increases both basal and liposome-enhanced ATPase activity, indicating that these elements play a role in autoinhibiting VPS4A until it encounters ESCRT-III polymers. The interactions between acidic ESCRT-III residues and sequences in VPS4A, in particular in the linker connecting the MIT and AAA+ domains, are involved in regulating the intrinsic autoinhibition of the enzyme.

Autoinhibitory domains (AIDs)

34-140 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q8VEJ9

Entry ID Method Resolution Chain Position Source
AF-Q8VEJ9-F1 Predicted AlphaFoldDB

13 variants for Q8VEJ9

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3388989463 30 R>S No EVA
rs3388959905 58 A>S No EVA
rs3389007134 61 M>V No EVA
rs3388985493 80 H>Q No EVA
rs3389013969 83 K>R No EVA
rs3389014841 137 E>D No EVA
rs3399812697 192 V>A No EVA
rs3389013912 217 R>W No EVA
rs3412885672 255 M>I No EVA
rs3388996960 256 Q>R No EVA
rs3388959928 267 V>I No EVA
rs3389013906 268 L>I No EVA
rs3389011123 283 R>S No EVA

No associated diseases with Q8VEJ9

3 regional properties for Q8VEJ9

Type Name Position InterPro Accession
domain Protein kinase domain 4 - 260 IPR000719
active_site Serine/threonine-protein kinase, active site 119 - 131 IPR008271
binding_site Protein kinase, ATP binding site 10 - 33 IPR017441

Functions

Description
EC Number 3.6.4.6 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Late endosome membrane ; Peripheral membrane protein
  • Midbody
  • Cytoplasm, cytoskeleton, spindle
  • Membrane-associated in the prevacuolar endosomal compartment
  • Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody
  • Localized in two distinct rings on either side of the Flemming body (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

18 GO annotations of cellular component

Name Definition
ATPase complex A protein complex which is capable of ATPase activity.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
early endosome A membrane-bounded organelle that receives incoming material from primary endocytic vesicles that have been generated by clathrin-dependent and clathrin-independent endocytosis; vesicles fuse with the early endosome to deliver cargo for sorting into recycling or degradation pathways.
endosome A vacuole to which materials ingested by endocytosis are delivered.
endosome membrane The lipid bilayer surrounding an endosome.
Flemming body A cell part that is the central region of the midbody characterized by a gap in alpha-tubulin staining. It is a dense structure of antiparallel microtubules from the central spindle in the middle of the intercellular bridge.
late endosome A prelysosomal endocytic organelle differentiated from early endosomes by lower lumenal pH and different protein composition. Late endosomes are more spherical than early endosomes and are mostly juxtanuclear, being concentrated near the microtubule organizing center.
late endosome membrane The lipid bilayer surrounding a late endosome.
lysosome A small lytic vacuole that has cell cycle-independent morphology found in most animal cells and that contains a variety of hydrolases, most of which have their maximal activities in the pH range 5-6. The contained enzymes display latency if properly isolated. About 40 different lysosomal hydrolases are known and lysosomes have a great variety of morphologies and functions.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
nuclear pore A protein complex providing a discrete opening in the nuclear envelope of a eukaryotic cell, where the inner and outer nuclear membranes are joined.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.
vacuolar membrane The lipid bilayer surrounding the vacuole and separating its contents from the cytoplasm of the cell.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
protein C-terminus binding Binding to a protein C-terminus, the end of a peptide chain at which the 1-carboxyl function of a constituent amino acid is not attached in peptide linkage to another amino-acid residue.
protein domain specific binding Binding to a specific domain of a protein.
protein-containing complex binding Binding to a macromolecular complex.
small GTPase binding Binding to a small monomeric GTPase.

34 GO annotations of biological process

Name Definition
abscission The controlled shedding of a body part.
autophagosome maturation Removal of PI3P and Atg8/LC3 after the closure of the phagophore and before the fusion with the endosome/lysosome (e.g. mammals and insects) or vacuole (yeast), and that very likely destabilizes other Atg proteins and thus enables their efficient dissociation and recycling.
autophagy The cellular catabolic process in which cells digest parts of their own cytoplasm; allows for both recycling of macromolecular constituents under conditions of cellular stress and remodeling the intracellular structure for cell differentiation.
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
endosomal transport The directed movement of substances mediated by an endosome, a membrane-bounded organelle that carries materials enclosed in the lumen or located in the endosomal membrane.
endosomal vesicle fusion The homotypic fusion of endocytic vesicles to form or add to an early endosome.
intracellular cholesterol transport The directed movement of cholesterol, cholest-5-en-3-beta-ol, within cells.
late endosomal microautophagy The autophagy process by which cytosolic proteins targeted for degradation are tagged with a chaperone and are directly transferred into and degraded in a late endosomal compartment.
late endosome to lysosome transport via multivesicular body sorting pathway The directed movement of substances from late endosomes to lysosomes by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the lysosome.
membrane fission A process that is carried out at the cellular level which results in the separation of a single continuous membrane into two membranes.
midbody abscission The process by which the midbody, the cytoplasmic bridge that connects the two prospective daughter cells, is severed at the end of mitotic cytokinesis, resulting in two separate daughter cells.
mitotic cytokinesis checkpoint signaling A signaling process that contributes to a mitotic cell cycle checkpoint that detects a defect in cytokinesis and prevents further rounds of nuclear division until cytokinesis is completed.
mitotic metaphase plate congression The cell cycle process in which chromosomes are aligned at the metaphase plate, a plane halfway between the poles of the mitotic spindle, during mitosis.
multivesicular body assembly The aggregation, arrangement and bonding together of a set of components to form a multivesicular body, a type of late endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.
multivesicular body sorting pathway A vesicle-mediated transport process in which transmembrane proteins are ubiquitylated to facilitate their entry into luminal vesicles of multivesicular bodies (MVBs); upon subsequent fusion of MVBs with lysosomes or vacuoles, the cargo proteins are degraded.
negative regulation of cell death Any process that decreases the rate or frequency of cell death. Cell death is the specific activation or halting of processes within a cell so that its vital functions markedly cease, rather than simply deteriorating gradually over time, which culminates in cell death.
negative regulation of cytokinesis Any process that stops, prevents, or reduces the frequency, rate or extent of the division of the cytoplasm of a cell, and its separation into two daughter cells.
nuclear membrane reassembly The reformation of the nuclear membranes following their breakdown in the context of a normal process.
nucleus organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the nucleus.
plasma membrane repair The resealing of a cell plasma membrane after cellular wounding due to, for instance, mechanical stress.
positive regulation of exosomal secretion Any process that activates or increases the frequency, rate or extent of exosomal secretion.
positive regulation of viral budding via host ESCRT complex Any process that activates or increases the frequency, rate or extent of viral budding via host ESCRT complex.
protein targeting to lysosome The process of directing proteins towards the lysosome using signals contained within the protein.
regulation of protein localization Any process that modulates the frequency, rate or extent of any process in which a protein is transported to, or maintained in, a specific location.
regulation of protein localization to plasma membrane Any process that modulates the frequency, rate or extent of protein localization to plasma membrane.
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway The chemical reactions and pathways resulting in the breakdown of a protein or peptide covalently tagged with ubiquitin, via the multivesicular body (MVB) sorting pathway; ubiquitin-tagged proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation.
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway The chemical reactions and pathways resulting in the breakdown of a protein or peptide, via the multivesicular body (MVB) sorting pathway; proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation. This process is independent of ubiquitination.
vacuole organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a vacuole.
vesicle budding from membrane The evagination of a membrane, resulting in formation of a vesicle.
vesicle uncoating A protein depolymerization process that results in the disassembly of vesicle coat proteins.
vesicle-mediated transport A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane.
viral budding from plasma membrane A viral budding that starts with formation of a membrane curvature in the host plasma membrane.
viral budding via host ESCRT complex Viral budding which uses a host ESCRT protein complex, or complexes, to mediate the budding process.
viral release from host cell The dissemination of mature viral particles from the host cell, e.g. by cell lysis or the budding of virus particles from the cell membrane.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P52917 VPS4 Vacuolar protein sorting-associated protein 4 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q0VD48 VPS4B Vacuolar protein sorting-associated protein 4B Bos taurus (Bovine) SS
O75351 VPS4B Vacuolar protein sorting-associated protein 4B Homo sapiens (Human) PR
Q9UN37 VPS4A Vacuolar protein sorting-associated protein 4A Homo sapiens (Human) EV
P46467 Vps4b Vacuolar protein sorting-associated protein 4B Mus musculus (Mouse) PR
Q8K0T4 Katnal1 Katanin p60 ATPase-containing subunit A-like 1 Mus musculus (Mouse) PR
Q793F9 Vps4a Vacuolar protein sorting-associated protein 4A Rattus norvegicus (Rat) PR
D0FH76 VPS4 Vacuolar protein sorting-associated protein 4 Bombyx mori (Silk moth) SS
Q9ZNT0 SKD1 Protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MTTSTLQKAI DLVTKATEED KAKNYEEALR LYQHAVEYFL HAIKYEAHSD KAKESIRAKC
70 80 90 100 110 120
MQYLDRAEKL KDYLRNKEKH GKKPVKENQS EGKGSDSDSE GDNPEKKKLQ EQLMGAVVME
130 140 150 160 170 180
KPNIRWNDVA GLEGAKEALK EAVILPIKFP HLFTGKRTPW RGILLFGPPG TGKSYLAKAV
190 200 210 220 230 240
ATEANNSTFF SVSSSDLMSK WLGESEKLVK NLFELARQHK PSIIFIDEVD SLCGSRNENE
250 260 270 280 290 300
SEAARRIKTE FLVQMQGVGN NNDGTLVLGA TNIPWVLDSA IRRRFEKRIY IPLPEEAARA
310 320 330 340 350 360
QMFRLHLGST PHNLTDANIH ELARKTEGYS GADISIIVRD SLMQPVRKVQ SATHFKKVCG
370 380 390 400 410 420
PSRTNPSVMI DDLLTPCSPG DPGAIEMTWM DVPGDKLLEP VVCMSDMLRS LATTRPTVNA
430
DDLLKVKKFS EDFGQES