Q8R550

SS
Gene name
Sh3kbp1 (Ruk, Seta)
Protein name
SH3 domain-containing kinase-binding protein 1
Names
Regulator of ubiquitous kinase, Ruk, SH3-containing, expressed in tumorigenic astrocytes
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:58194
EC number
Protein Class

Descriptions

The CIN85 (Cbl-interacting protein of 85 kDa) is a multifunction adapter or scaffolding protein consisting of three SH3 domains in the N-terminal half of the protein connected by unstructured linkers followed by a proline rich region, a serine rich region, and a coiled coil domain at the C-terminus and plays important roles in both receptor tyrosine kinase downregulation and phosphatidylinositol 3 kinase inhibition. Prevention of the intramolecular interaction of domain C with the proline-rich motif P1 results in the complex formation of CIN85 with specific targets such as Cbl or p85 proteins.

Autoinhibitory domains (AIDs)

381-390 (Proline rich motif P1) SS

Target domain

315-370 (SH3 domain-containing kinase-binding protein 1, third SH3 domain)

Relief mechanism

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

4 structures for Q8R550

Entry ID Method Resolution Chain Position Source
1WI7 NMR - A 101-155 PDB
2DA9 NMR - A 314-370 PDB
5XHZ X-ray 132 A A/B 98-157 PDB
AF-Q8R550-F1 Predicted AlphaFoldDB

3 variants for Q8R550

Variant ID(s) Position Change Description Diseaes Association Provenance
rs29304756 77 V>L No Ensembl
rs29307875 210 T>M No Ensembl
rs247465429 601 A>S No Ensembl

No associated diseases with Q8R550

5 regional properties for Q8R550

Type Name Position InterPro Accession
domain Cation-transporting P-type ATPase, N-terminal 116 - 191 IPR004014
domain Cation-transporting P-type ATPase, C-terminal 841 - 1015 IPR006068
ptm P-type ATPase, phosphorylation site 456 - 462 IPR018303
domain Calcium-transporting P-type ATPase, N-terminal autoinhibitory domain 5 - 50 IPR024750
domain P-type ATPase, haloacid dehalogenase domain 436 - 808 IPR044492

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Cytoplasm, cytoskeleton
  • Cytoplasmic vesicle membrane ; Peripheral membrane protein
  • Synapse, synaptosome
  • Cell junction, focal adhesion
  • Localized in endocytic vesicles containing clustered receptors
  • Colocalizes with ASAP1 in vesicular structures
  • Colocalized with actin microfilaments and focal adhesions (By similarity)
  • Colocalized with MAGI2 in synaptosomes (By similarity)
  • Translocation to EGFR containing vesicles upon EGF stimulation is inhibited in the presence of SH3KBP1 (PubMed:21830225)
  • Colocalizes with ZFP36 in the cytoplasm (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

8 GO annotations of cellular component

Name Definition
cell-cell junction A cell junction that forms a connection between two or more cells of an organism; excludes direct cytoplasmic intercellular bridges, such as ring canals in insects.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic vesicle membrane The lipid bilayer surrounding a cytoplasmic vesicle.
cytoskeleton A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
endocytic vesicle A membrane-bounded intracellular vesicle formed by invagination of the plasma membrane around an extracellular substance. Endocytic vesicles fuse with early endosomes to deliver the cargo for further sorting.
focal adhesion A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
neuron projection A prolongation or process extending from a nerve cell, e.g. an axon or dendrite.
synapse The junction between an axon of one neuron and a dendrite of another neuron, a muscle fiber or a glial cell. As the axon approaches the synapse it enlarges into a specialized structure, the presynaptic terminal bouton, which contains mitochondria and synaptic vesicles. At the tip of the terminal bouton is the presynaptic membrane; facing it, and separated from it by a minute cleft (the synaptic cleft) is a specialized area of membrane on the receiving cell, known as the postsynaptic membrane. In response to the arrival of nerve impulses, the presynaptic terminal bouton secretes molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane.

2 GO annotations of molecular function

Name Definition
SH3 domain binding Binding to a SH3 domain (Src homology 3) of a protein, small protein modules containing approximately 50 amino acid residues found in a great variety of intracellular or membrane-associated proteins.
ubiquitin protein ligase binding Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.

7 GO annotations of biological process

Name Definition
actin filament organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments. Includes processes that control the spatial distribution of actin filaments, such as organizing filaments into meshworks, bundles, or other structures, as by cross-linking.
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues. Cell migration is a central process in the development and maintenance of multicellular organisms.
cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures.
endocytosis A vesicle-mediated transport process in which cells take up external materials or membrane constituents by the invagination of a small region of the plasma membrane to form a new membrane-bounded vesicle.
positive regulation of B cell activation Any process that activates or increases the frequency, rate or extent of B cell activation.
regulation of cell shape Any process that modulates the surface configuration of a cell.

10 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9Y5K6 CD2AP CD2-associated protein Homo sapiens (Human) SS
Q96B97 SH3KBP1 SH3 domain-containing kinase-binding protein 1 Homo sapiens (Human) EV
Q8BZT2 Sh3rf2 E3 ubiquitin-protein ligase SH3RF2 Mus musculus (Mouse) PR
Q9JK48 Sh3glb1 Endophilin-B1 Mus musculus (Mouse) PR
Q62420 Sh3gl2 Endophilin-A1 Mus musculus (Mouse) PR
Q62419 Sh3gl1 Endophilin-A2 Mus musculus (Mouse) PR
Q62421 Sh3gl3 Endophilin-A3 Mus musculus (Mouse) PR
Q9JLQ0 Cd2ap CD2-associated protein Mus musculus (Mouse) SS
F1LRS8 Cd2ap CD2-associated protein Rattus norvegicus (Rat) SS
Q925Q9 Sh3kbp1 SH3 domain-containing kinase-binding protein 1 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MVEAIVEFDY QAQHDDELTI SVGEVITNIR KEDGGWWEGQ INGRRGLFPD NFVREIKKDM
70 80 90 100 110 120
KKDLLSNKAP EKPMHDVSSG NALLSSETIL RTNKRGERRR RRCQVAFSYL PQNDDELELK
130 140 150 160 170 180
VGDIIEVVGE VEEGWWEGVL NGKTGMFPSN FIKELSGESD ELGISQDEQL SKSRPEGFLP
190 200 210 220 230 240
ASLLPFPAHG AKGKTTFEGT ILYRAAPGKT EGHRRYYSLR ETTGSESDGG DSSSTKSEGA
250 260 270 280 290 300
NGTMATAAIQ PKKVKGVGFG DIFKDKPIKL RPRSIEVEND FLPVEKTIGK KLPPATSTPD
310 320 330 340 350 360
PSKTEMDSRT KTKDYCKVIF PYEAQNDDEL TIKEGDIVTL INKDCIDVGW WEGELNGRRG
370 380 390 400 410 420
VFPDNFVKLL PSDFDKEGNR PKKPPPPSAP VVKQGAGTTE RKHEIKKIPP ERPETLPNRT
430 440 450 460 470 480
EEKERPEREP KLDLQKPSVP AIPPKKPRPP KTNSLNRPGA LPPRRPERPV GPLTHTRGDS
490 500 510 520 530 540
PKIDLAGSAL SGILDKDLSD RSNDIDLEGF DSVISSTEKL SHPTTSRPKA TGRRPPSQSL
550 560 570 580 590 600
TSSSLSSPDI FDSPSPEEDK EEHISLAHRG IDVSKKTSKT VTISQVSDNK TSLPPKPGTM
610 620 630 640 650 660
AAASSGPASL SSVASSPMSS SLGTAGQRAS SPSLFSTEGK PKMEPAVSSQ AAIEELKMQV
670 680 690 700
RELRTIIETM KDQQKREIKQ LLSELDEEKK IRLRLQMEVN DIKKALQSK